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Source: Experimental Cell Research

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Total 221 results found since Jan 2013.

Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch
Exp Cell Res. 2021 Apr 15:112598. doi: 10.1016/j.yexcr.2021.112598. Online ahead of print.ABSTRACTDuring orthodontic treatment, mechanical stretch serves a crucial function in osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Up-regulated reactive oxygen species (ROS) level is a result of cyclic mechanical stretch in many cell types. Nuclear factor erythroid-2-related factor-2 (Nrf2) is a master regulator in various antioxidants expression. However, it is not known whether cyclic mechanical stretch could induce the ROS generation in PDLSCs and whether Nrf2 participated in this process. The present stu...
Source: Experimental Cell Research - April 18, 2021 Category: Cytology Authors: Xun Xi Yi Zhao Hong Liu Zixuan Li Shuai Chen Dongxu Liu Source Type: research

Protective Effect of SIRT6 on Cholesterol Crystal-induced Endothelial Dysfunction via Regulating ACE2 Expression
Exp Cell Res. 2021 Feb 22:112526. doi: 10.1016/j.yexcr.2021.112526. Online ahead of print.ABSTRACTSirtuins are a family of highly conserved nicotinamide adenine dinucleotide (NAD+)-dependent enzymes. Among the sirtuins, SIRT1 and SIRT6 participate in the regulation of endothelial functions and play significant roles in the physiological and pathological processes of cardiovascular diseases (CVD). Recently, our study found that minute cholesterol crystals (CC) can be endocytosed by endothelial cells and further impair endothelial functions. Since previous studies have reported that angiotensin-converting enzyme (ACE2) invol...
Source: Experimental Cell Research - February 25, 2021 Category: Cytology Authors: Zihan Zheng Wang Bo Xiaohan Lv Feng Yao Hongqian Gao Zhen Jin Yizhen Liu Ying Deng Danli Chen Xuan Ning Weirong Wang Jianyu He Rong Lin Source Type: research

Involvement of TFAP2A in the activation of GSDMD gene promoter in hyperoxia-induced ALI
Exp Cell Res. 2021 Feb 17:112521. doi: 10.1016/j.yexcr.2021.112521. Online ahead of print.ABSTRACTOxygen therapy is a common treatment in neonatal intensive care units, but long-term continuous hyperoxia ventilation may induce acute lung injury (ALI). Gasdermin D (GSDMD)-mediated pyroptosis participates in various diseases including ALI, but the role of GSDMD in hyperoxia-induced ALI is yet understood. Here, we showed a significant increase of GSDMD after exposure to high oxygen. To elucidate the molecular mechanisms involved in GSDMD regulation, we identified the core promoter of GSDMD, -98 ∼ -12 bp relative to the tran...
Source: Experimental Cell Research - February 20, 2021 Category: Cytology Authors: Qian Cao Dandan Feng Jia He Lanbo Zhou Zhidan Fan Yufei Chen Xiaoqing Chen Rui Jin Guoping Zhou Source Type: research

Involvement of TFAP2A in the activation of GSDMD gene promoter in hyperoxia-induced ALI.
Abstract Oxygen therapy is a common treatment in neonatal intensive care units, but long-term continuous hyperoxia ventilation may induce acute lung injury (ALI). Gasdermin D (GSDMD)-mediated pyroptosis participates in various diseases including ALI, but the role of GSDMD in hyperoxia-induced ALI is yet understood. Here, we showed a significant increase of GSDMD after exposure to high oxygen. To elucidate the molecular mechanisms involved in GSDMD regulation, we identified the core promoter of GSDMD, -98 ∼ -12 bp relative to the transcriptional start site (TSS). The results of mutational analysis, overexpression...
Source: Experimental Cell Research - February 17, 2021 Category: Cytology Authors: Cao Q, Feng D, He J, Zhou L, Fan Z, Chen Y, Chen X, Jin R, Zhou G Tags: Exp Cell Res Source Type: research

TMEM2 binds to CSNK2A3 to inhibit HBV infection via activation of the JAK/STAT pathway.
In conclusion, TMEM2 has been determined to bind to CSNK2A3 to inhibit HBV infection via activation of the JAK-STAT pathway. PMID: 33582094 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - February 11, 2021 Category: Cytology Authors: Zhou H, Jia X, Hu K, Mo Z, Xu W, Peng L, Wang K, Zhu X Tags: Exp Cell Res Source Type: research

High expression of Notch2 drives tongue squamous cell carcinoma carcinogenesis.
Abstract Tongue squamous cell carcinoma (TSCC) is one of the most common cancers in the oral cavity. Notch signaling is frequently dysregulated in cancer. However, the role of Notch2 in TSCC is not well understood. The aim of this study was to investigate the effect of abnormal expression of Notch2 in TSCC. The expression of Notch2 was tested in 47 pairs of tissues from tongue cancer and normal samples by using immunohistochemical staining. Tongue cancer cells were transfected with siRNA or plasmid. The proliferation of the cells was tested by the CCK8 assay and colony formation assay. Subcutaneous tumor model was...
Source: Experimental Cell Research - December 28, 2020 Category: Cytology Authors: Gan RH, Lin LS, Zheng DP, Zhao Y, Ding LC, Zheng DL, Lu YG Tags: Exp Cell Res Source Type: research

Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes.
Conclusion: PA promotes the Tyr353 phosphorylation of IP3R1 by activating the src pathway and increasing the protein stability of IP3R1, which consequently results in mitochondrial Ca2+ overload and mitochondrial dysfunction in hepatic cells. Our results also suggested that inhibition of the src/IP3R1 pathway, such as by SU6656, may be a novel potential therapeutic approach for the treatment of NAFLD. PMID: 33358861 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - December 21, 2020 Category: Cytology Authors: Yu T, Zheng E, Li Y, Li Y, Xia J, Ding Q, Hou Z, Ruan XZ, Zhao L, Chen Y Tags: Exp Cell Res Source Type: research

LncRNA embryonic stem cells expressed 1 (Lncenc1) is identified as a novel regulator in neuropathic pain by interacting with EZH2 and downregulating the expression of BAI1 in mouse microglia.
In conclusion, Lncenc1 contributes to neuropathic pain by interacting with EZH2 and downregulating the BAI1 gene in mouse microglia. PMID: 33340495 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - December 16, 2020 Category: Cytology Authors: Zhang Z, Sun X, Zhao G, Ma Y, Zeng G Tags: Exp Cell Res Source Type: research

NIMA-related kinase 7 amplifies NLRP3 inflammasome pro-inflammatory signaling in microglia/macrophages and mice models of spinal cord injury.
CONCLUSION: NEK7 amplifies NLRP3 inflammasome pro-inflammatory signaling in BV-2 microglia cells and mice models of SCI. Therefore, agents targeting the NEK7/NLRP3 signaling offers great promise in the treatment of inflammatory response post-SCI. PMID: 33309808 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - December 9, 2020 Category: Cytology Authors: Ji X, Song Z, He J, Guo S, Chen Y, Wang H, Zhang J, Xu X, Liu J Tags: Exp Cell Res Source Type: research

Activation of the CaR-CSE/H2S pathway confers cardioprotection against ischemia-reperfusion injury.
Abstract Ischemia-reperfusion (I/R) injury is a multifactorial process triggered when an organ is subjected to transiently reduced blood supply. The result is a cascade of pathological complications and organ damage due to the production of reactive oxygen species following reperfusion. The present study aims to evaluate the role of activated calcium-sensing receptor (CaR)-cystathionine γ-lyase (CSE)/hydrogen sulfide (H2S) pathway in I/R injury. Firstly, an I/R rat model with CSE knockout was constructed. Transthoracic echocardiography, TTC and HE staining were performed to determine the cardiac function of rats ...
Source: Experimental Cell Research - November 19, 2020 Category: Cytology Authors: Luo Y, Liu LM, Xie L, Zhao HL, Lu YK, Wu BQ, Wu ZY, Zhang ZL, Hao YL, Ou WH, Liu RS, Xu WM, Chen XH Tags: Exp Cell Res Source Type: research

siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.
In this study, we focused on alanine aminopeptidase (APP) and investigated the function of APP in C2C12 myoblast proliferation and differentiation. In myoblasts and myotubes, APP was mainly localized in the cell membrane as well as expressed at low levels in the cytoplasm and nucleus. The reduction of the APP enzymatic activity impaired the cell cycle progression in C2C12 myoblasts. In addition, apoptosis was induced after APP-knockdown. Finally, myogenic differentiation was also delayed in the APP-suppressed myoblasts. These findings indicate that APP is required for myoblast proliferation and differentiation. PMID: ...
Source: Experimental Cell Research - October 19, 2020 Category: Cytology Authors: Osana S, Kitajima Y, Suzuki N, Xu Y, Murayama K, Nagatomi R Tags: Exp Cell Res Source Type: research

Bleomycin induced apical-basal polarity loss in alveolar epithelial cell contributes to experimental pulmonary fibrosis.
Abstract Idiopathic pulmonary fibrosis (IPF) is a fatal fibrosing interstitial lung disease with limited therapeutic options and a median survival of 3 years after diagnosis. Dysregulated epithelial regeneration is key event involved in initiating and sustaining IPF. The type II alveolar epithelial cells (AECIIs) play a crucial role for epithelial regeneration and stabilisation of alveoli. Loss of cell apical-basal polarity contributes to fibrosis. AECII has apical-basal polarity, but it is poorly understood whether AECII apical-basal polarity loss is involved in fibrosis. Bleomycin is a traditional inducer of pul...
Source: Experimental Cell Research - September 20, 2020 Category: Cytology Authors: Lu YZ, He XL, Liu F, Cheng PP, Liang LM, Wang M, Chen SJ, Huang Y, Yu F, Xin JB, Ye H, Song LJ, Ma WL Tags: Exp Cell Res Source Type: research

Prognostic and therapeutic relevance of phosphofructokinase platelet-type (PFKP) in breast cancer.
Abstract In the present study, we have explored the prognostic value of the Phosphofructokinase Platelet-type (PFKP) expression and its therapeutic relevance in metastatic breast cancer. PFKP immunohistochemistry was performed on Invasive ductal carcinomas (IDCs; n = 87) of breast, and its association with clinicopathological parameters were evaluated. Using online meta-analysis tools, PFKP's prognostic value was investigated in overall breast cancer as well as in triple negative subtype (TNBCs). For in vitro analysis, MDA-MB-231 cells model was used in order to elucidate mechanisms behind PFKP regulated gly...
Source: Experimental Cell Research - September 9, 2020 Category: Cytology Authors: Umar SM, Kashyap A, Kahol S, Mathur S, Gogia A, Deo SVS, Prasad CP Tags: Exp Cell Res Source Type: research