Filtered By:
Source: Experimental Cell Research

This page shows you your search results in order of date. This is page number 13.

Order by Relevance | Date

Total 221 results found since Jan 2013.

Regulation of High glucose-mediated mucin expression by matrix metalloproteinase-9 in Human airway epithelial cells.
Abstract Mucus hypersecretion is the key manifestation in patients with chronic inflammatory airway diseases and mucin 5AC (MUC5AC) is a major component of airway mucus. Matrix metalloproteinases (MMP)-9, have been found to be involved in the pathogenesis of inflammatory airway diseases. Hyperglycemia has been shown to be an independent risk factor for respiratory infections. We hypothesize that high glucose (HG)-regulates MMP-9 production and MMP-9 activity through nicotinamide adenine dinucleotide phosphate (NADPH)/ reactive oxygen species (ROS) cascades pathways, leading to mucin production in human airway epit...
Source: Experimental Cell Research - February 19, 2015 Category: Cytology Authors: Yu H, Yang J, Xiao Q, Lü Y, Zhou X, Xia L, Nie D Tags: Exp Cell Res Source Type: research

The sGC activator inhibits the proliferation and migration, promotes the apoptosis of Human pulmonary arterial smooth muscle cells via the up regulation of plasminogen activator inhibitor-2.
CONCLUSIONS: PAI-2 overexpression inhibits the proliferation and migration, promotes the apoptosis of human PASMCs. Therefore, sGC activator might alleviate or reverse vascular remodeling in PH through the up-regulation of PAI-2. PMID: 25704756 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - February 19, 2015 Category: Cytology Authors: Zhang S, Zou L, Yang T, Yang Y, Zhai Z, Xiao F, Wang C Tags: Exp Cell Res Source Type: research

The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells.
In this study we demonstrated that in PC12 cells the TrkA receptor interacts with the deubiquitinating enzyme USP8/UBPy in a NGF-dependent manner, and that it is deubiquitinated in vivo and in vitro by USP8. USP8 overexpression blocked NGF-induced neurites outgrowth while the overexpression of the catalytically inactive mutant USP8/UBPy(C748A) caused a marked increase of cell differentiation. Localization and biochemical experiments have point out that USP8 and TrkA partially co-localize in endosomes after NGF stimulation. Finally we have studied the role played by USP8 on TrkA turnover; using specific siRNA for USP8 we fo...
Source: Experimental Cell Research - February 4, 2015 Category: Cytology Authors: Ceriani M, Amigoni L, D'Aloia A, Berruti G, Martegani E Tags: Exp Cell Res Source Type: research

Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells.
In this study, MMP-3 small interfering RNA (siRNA) was transfected into odontoblast-like cells derived from induced pluripotent stem cells to investigate whether MMP-3 activity is induced by Poly(P) and/or is associated with cell proliferation and differentiation into odontoblast-like cells. Treatment with Poly(P) led to an increase in both cell proliferation and additional odontoblastic differentiation. Poly(P)-treated cells showed a small but significant increase in dentin sialophosphoprotein (DSPP) and dentin matrix protein-1 (DMP-1) mRNA expression, which are markers of mature odontoblasts. The cells also acquired addi...
Source: Experimental Cell Research - February 3, 2015 Category: Cytology Authors: Ozeki N, Hase N, Yamaguchi H, Hiyama T, Kawai R, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

Differenttially Expressed Proteins In Human Mcf-7 Breast Cancer Cells Sensitive And Resistant To Paclitaxel.
Abstract Resistance of cancer cells to chemotherapeutic agents is one of the main causes of treatment failure. In order to detect proteins potentially involved in the mechanism of resistance to taxanes, we assessed differences in protein expression in MCF-7 breast cancer cells that are sensitive to paclitaxel and in the same cells with acquired resistance to paclitaxel (established in our lab). Proteins were separated using two-dimensional electrophoresis. Changes in their expression were determined and proteins with altered expression were identified using mass spectrometry. Changes in their expression were confi...
Source: Experimental Cell Research - December 31, 2014 Category: Cytology Authors: Pavlíková N, Bartoňová I, Balušíková K, Halada P, Kovář J Tags: Exp Cell Res Source Type: research

Characterization of the sebocyte lipid droplet proteome reveals novel potential regulators of sebaceous lipogenesis.
Abstract Lipid metabolism depends on lipid droplets (LD), cytoplasmic structures surrounded by a protein-rich phospholipid monolayer. Although lipid synthesis is the hallmark of sebaceous gland cell differentiation, the LD-associated proteins of sebocytes have not been evaluated systematically. The LD fraction of SZ95 sebocytes was collected by density gradient centrifugation and associated proteins were analyzed by nanoliquid chromatography/tandem mass spectrometry. 54 proteins were significantly enriched in LD fractions, and 6 of them have not been detected previously in LDs. LD fractions contained high levels o...
Source: Experimental Cell Research - December 16, 2014 Category: Cytology Authors: Dahlhoff M, Fröhlich T, Arnold GJ, Müller U, Leonhardt H, Zouboulis CC, Schneider MR Tags: Exp Cell Res Source Type: research

BCL-2 family protein, BAD is down-regulated in breast Cancer and inhibits Cancer cell invasion.
Abstract We have previously demonstrated that the anti-apoptotic protein BAD is expressed in normal human breast tissue and shown that BAD inhibits expression of cyclin D1 to delay cell-cycle progression in breast cancer cells. Herein, expression of proteins in breast tissues was studied by immunohistochemistry and results were analyzed statistically to obtain semi-quantitative data. Biochemical and functional changes in BAD-overexpressing MCF7 breast cancer cells were evaluated using PCR, reporter assays, western blotting, ELISA and extracellular matrix invasion assays. Compared to normal tissues, Grade II breast...
Source: Experimental Cell Research - December 8, 2014 Category: Cytology Authors: Cekanova M, Fernando RI, Siriwardhana N, Sukhthankar M, Parra CD, Woraratphoka J, Malone C, Ström A, Baek SJ, Wade PA, Saxton AM, Donnell RM, Pestell RG, Dharmawardhane S, Wimalasena J Tags: Exp Cell Res Source Type: research

Ascribing novel functions to the sarcomeric protein, myosin binding protein H (MyBPH) in cardiac sarcomere contraction.
This study provides evidence for a potential role of MyBPH in HCM, which warrants further investigation. PMID: 25449695 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - November 18, 2014 Category: Cytology Authors: Mouton J, Loos B, Moolman-Smook H, Kinnear C Tags: Exp Cell Res Source Type: research

Unique proliferation response in odontoblastic cells derived from human skeletal muscle stem cells by cytokine-induced matrix metalloproteinase-3.
Abstract A pro-inflammatory cytokine mixture (CM: interleukin (IL)-1β,tumor necrosis factor-α and interferon-γ) and IL-1β-induced matrix metalloproteinase (MMP)-3 activity have been shown to increase the proliferation of rat dental pulp cells and murine stem cell-derived odontoblast-like cells. This suggests that MMP-3 may regulate wound healing and regeneration in the odontoblast-rich dental pulp. Here, we determined whether these results can be extrapolated to human dental pulp by investigating the effects of CM-induced MMP-3 up-regulation on the proliferation and apoptosis of purified odontoblast-like cells...
Source: Experimental Cell Research - September 22, 2014 Category: Cytology Authors: Ozeki N, Hase N, Kawai R, Yamaguchi H, Hiyama T, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

Lysosome-associated membrane proteins (LAMPs) regulate intracellular positioning of mitochondria in MC3T3-E1 cells.
Abstract The intracellular positioning of both lysosomes and mitochondria meets the requirements of degradation and energy supply, which are respectively the two major functions for cellular maintenance. The positioning of both lysosomes and mitochondria is apparently affected by the nutrient status of the cells. However, the mechanism coordinating the positioning of the organelles has not been sufficiently elucidated. Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) are highly glycosylated proteins that are abundant in lysosomal membranes. In the present study, we demonstrated that the siRNA-med...
Source: Experimental Cell Research - September 22, 2014 Category: Cytology Authors: Rajapakshe AR, Podyma-Inoue KA, Terasawa K, Hasegawa K, Namba T, Kumei Y, Yanagishita M, Hara-Yokoyama M Tags: Exp Cell Res Source Type: research

Suppression of autophagy augments the radiosensitizing effects of STAT3 inhibition on Human glioma cells.
In conclusion, our data revealed a protective role of autophagy in the radiosensitizing effects of STAT3 inhibition, and both inhibition of autophagy and STAT3 might be a potential therapeutic strategy to increase the radiosensitivity of glioma cells. PMID: 25220423 [PubMed - as supplied by publisher]
Source: Experimental Cell Research - September 11, 2014 Category: Cytology Authors: Yuan X, Du J, Hua S, Zhang H, Gu C, Wang J, Yang L, Huang J, Yu J, Liu F Tags: Exp Cell Res Source Type: research

α2 integrin, extracellular matrix metalloproteinase inducer, and matrix metalloproteinase-3 act sequentially to induce differentiation of mouse embryonic stem cells into odontoblast-like cells.
Abstract We previously reported that interleukin 1β acts via matrix metalloproteinase (MMP)-3 to regulate cell proliferation and suppress apoptosis in α2 integrin-positive odontoblast-like cells differentiated from mouse embryonic stem (ES) cells. Here we characterize the signal cascade underpinning odontoblastic differentiation in mouse ES cells. The expression of α2 integrin, extracellular matrix metalloproteinase inducer (Emmprin), and MMP-3 mRNA and protein were all potently increased during odontoblastic differentiation. Small interfering RNA (siRNA) disruption of the expression of these effectors potently...
Source: Experimental Cell Research - September 3, 2014 Category: Cytology Authors: Ozeki N, Kawai R, Hase N, Hiyama T, Yamaguchi H, Kondo A, Nakata K, Mogi M Tags: Exp Cell Res Source Type: research

Sphingosine-1-phosphate Mediates epidermal growth factor-induced muscle satellite cell activation.
Abstract Skeletal muscle can regenerate repeatedly due to the presence of resident stem cells, called satellite cells. Because satellite cells are usually quiescent, they must be activated before participating in muscle regeneration in response to stimuli such as injury, overloading, and stretch. Although satellite cell activation is a crucial step in muscle regeneration, little is known of the molecular mechanisms controlling this process. Recent work showed that the bioactive lipid sphingosine-1-phosphate (S1P) plays crucial roles in the activation, proliferation, and differentiation of muscle satellite cells. W...
Source: Experimental Cell Research - June 21, 2014 Category: Cytology Authors: Nagata Y, Ohashi K, Wada E, Yuasa Y, Shiozuka M, Nonomura Y, Matsuda R Tags: Exp Cell Res Source Type: research

The different Roles of myosin IIA and myosin IIB in contraction of 3D collagen matrices by Human Fibroblasts.
Abstract Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model -- all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fibers and subsequently undergo spreading. During stressed matrix contraction, cells initially are spread with stress fibers and subsequently undergo shortening. In the current studies, we used siRNA silencing of myosin IIA (MyoIIA) and myosin IIB (MyoIIB) to test the role of myosin II isoforms i...
Source: Experimental Cell Research - April 24, 2014 Category: Cytology Authors: Liu Z, Ho CH, Grinnell F Tags: Exp Cell Res Source Type: research

High glucose increases Cdk5 activity in podocytes Via transforming growth factor-β1 signaling pathway.
In this study, we showed that high glucose up-regulated the expression of Cdk5 and its co-activator p35 with a concomitant increase in Cdk5 kinase activity in conditionally immortalized mouse podocytes in vitro. When exposed to 30mM glucose, transforming growth factor-β1 (TGF-β1) was activated. Most importantly, we found that SB431542, the Tgfbr1 inhibitor, significantly decreased the expression of Cdk5 and p35 and Cdk5 kinase activity in high glucose-treated podocytes. Moreover, high glucose increased the expression of early growth response-1 (Egr-1) via TGF-β1-ERK1/2 pathway in podocytes and inhibition of Egr-1 by siR...
Source: Experimental Cell Research - April 24, 2014 Category: Cytology Authors: Zhang Y, Li H, Hao J, Zhou Y, Liu W Tags: Exp Cell Res Source Type: research