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Condition: Osteoporosis

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Total 136 results found since Jan 2013.

Identification of C-terminal Hsp70-interacting protein as a mediator of tumour necrosis factor action in osteoblast differentiation by targeting osterix for degradation.
Abstract In patients with inflammatory arthritis, tumour necrosis factor (TNF)-α are overproduced in inflamed joints. This leads to local erosion of cartilage and bone, periarticular osteopenia, as well as osteoporosis. But less is known regarding the molecular mechanisms that mediate the effect of TNF-α on osteoblast function. The purpose of this study was to test that C terminus of Hsc70-interacting protein (CHIP) has a specific role in suppressing the osteogenic activity of osteoblasts under inflammatory conditions. C2C12, MC3T3-E1 and HEK293T cell lines were cultured and cotransfected with related plasmids. ...
Source: J Cell Mol Med - March 26, 2015 Category: Molecular Biology Authors: Xie J, Gu J Tags: J Cell Mol Med Source Type: research

Indole-3-carbinol as inhibitors of glucocorticoid-induced apoptosis in osteoblastic cells through blocking ROS-mediated Nrf2 pathway.
In this study, we showed that, indole-3-carbinol (I3C), a natural product found in broadly consumed plants of the Brassica genus, could block the cytotoxic effects of dexamethasone (Dex), and elucidated the underlying molecular mechanisms. Firstly, we showed that, I3C could effectively suppress Dex-induced cytotoxicity and apoptotic cell death in osteoblastic cells, as evidenced by the decrease in Sub-G1 cell population. Treatment of the cells with Dex resulted in activation of caspase-3/-8/-9 and subsequent cleavage of PARP, which was also effectively blocked by co-incubation of I3C. Moreover, exposure to Dex triggered a ...
Source: Biochemical and Biophysical Research communications - March 17, 2015 Category: Biochemistry Authors: Lin H, Gao X, Chen G, Sun J, Chu J, Jing K, Li P, Zeng R, Wei B Tags: Biochem Biophys Res Commun Source Type: research

Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia
Publication date: 15 March 2015 Source:Molecular and Cellular Endocrinology, Volume 404 Author(s): Juan-Li Yan , Jian Zhou , Hui-Ping Ma , Xiao-Ni Ma , Yu-Hai Gao , Wen-Gui Shi , Qing-Qing Fang , Qian Ren , Cory J. Xian , Ke-Ming Chen Although pulsed electromagnetic fields (PEMFs) have been approved as a therapy for osteoporosis, action mechanisms and optimal parameters are elusive. To determine the optimal intensity, exposure effects of 50 Hz PEMFs of 0.6–3.6 mT (0.6 interval at 90 min/day) were investigated on proliferation and osteogenic differentiation of cultured calvarial osteoblasts. All intensity groups sti...
Source: Molecular and Cellular Endocrinology - March 10, 2015 Category: Endocrinology Source Type: research

The Achilles’ Heel of Senescent Cells: From Transcriptome to Senolytic Drugs
This article is protected by copyright. All rights reserved.
Source: Aging Cell - March 9, 2015 Category: Cytology Authors: Yi Zhu, Tamara Tchkonia, Tamar Pirtskhalava, Adam Gower, Husheng Ding, Nino Giorgadze, Allyson K. Palmer, Yuji Ikeno, Gene Borden, Marc Lenburg, Steven P. O'Hara, Nicholas F. LaRusso, Jordan D. Miller, Carolyn M. Roos, Grace C. Verzosa, Nathan K. LeBrasse Tags: Original Article Source Type: research

Anabolic Bone Formation Via a Site‐Specific Bone‐Targeting Delivery System by Interfering With Semaphorin 4d Expression
ABSTRACT Semaphorins have been recently targeted as new molecules directly implicated in the cell‐cell communication that occurs between osteoclasts and osteoblasts. Overexpression of certain semaphorins, such as semaphorin4D (sema4D), is found in an osteoporotic phenotype and plays a key role in osteoclast activity by suppressing osteoblast maturation, thus significantly altering the bone modeling cycle. In the present study, we fabricate a site‐specific bone‐targeting drug‐delivery system from polymeric nanoparticles with the incorporation of siRNA interference molecule for sema4D and demonstrate their cellular u...
Source: Journal of Bone and Mineral Research - January 22, 2015 Category: Orthopaedics Authors: Yufeng Zhang, Lingfei Wei, Richard J Miron, Bin Shi, Zhuan Bian Tags: Original Article Source Type: research

PTH Blocks Osteoblast Apoptosis after Radiation Signal Transduction
We reported previously that daily injection of parathyroid hormone (PTH, 1–34) alleviates radiation-induced osteopenia in a preclinical radiotherapy model by improving osteoblast survival. To elucidate the molecular mechanisms, we irradiated osteoblastic UMR 106-01 cells and calvarial organ culture and demonstrated an anti-apoptosis effect of PTH1–34 on these cultures. Inhibitor assay indicated that PTH exerts its radioprotective action mainly through protein kinase A/β-catenin pathway. γ-H2AX foci staining and comet assay revealed that PTH efficiently promotes the repair of DNA double strand breaks (DSBs) in irradia...
Source: Journal of Biological Chemistry - January 2, 2015 Category: Chemistry Authors: Chandra, A., Lin, T., Zhu, J., Tong, W., Huo, Y., Jia, H., Zhang, Y., Liu, X. S., Cengel, K., Xia, B., Qin, L. Tags: Molecular Bases of Disease Source Type: research

Increased RANKL-mediated osteoclastogenesis by interleukin-1β and endoplasmic reticulum stress
Conclusions Osteoclastogenesis by IL-1β and/or ER stress is mainly associated with upregulation of eIF2α, GRP78, PERK and IRE1. These results suggest that the signaling pathway of ER stress-induced osteoclast formation might be a new therapeutic target to prevent inflammatory and destructive arthritic disease such as RA and diverse osteoporosis.
Source: Joint Bone Spine - December 12, 2014 Category: Orthopaedics Source Type: research

Protective effects of resveratrol on postmenopausal osteoporosis: regulation of SIRT1-NF-κB signaling pathway.
This study suggested the therapeutic potential of resveratrol against osteoporosis and stressed the importance of effective doses. PMID: 25377437 [PubMed - as supplied by publisher]
Source: Acta Biochimica et Biophysica Sinica - November 6, 2014 Category: Biochemistry Authors: Feng J, Liu S, Ma S, Zhao J, Zhang W, Qi W, Cao P, Wang Z, Lei W Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Functional analyses reveal the essential role of SOX6 and RUNX2 in the communication of chondrocyte and osteoblast
Conclusions Our study first reveals that SOX6 and RUNX2 play important roles in the chondrogenesis–osteogenesis coordination. This finding enriches the limited understanding about this coordination and unravels the novel function of SOX6 and RUNX2 in the endochondral ossification.
Source: Osteoporosis International - September 12, 2014 Category: Orthopaedics Source Type: research

Anabolic Bone Formation Via a Site Specific Bone Targeting Delivery System by Interfering with Semaphorin 4D Expression
Abstract Recently semaphorins have been targeted as new molecules directly implicated in the cell‐cell communication that occurs between osteoclasts and osteoblasts. Over‐expression of certain semaphorins such as semaphorin4D (sema4D) is found in an osteoporotic phenotype and plays a key role in osteoclast activity by suppressing osteoblast maturation, thus significantly altering the bone modelling cycle. In the present study, we fabricate a site‐specific bone‐targeting drug delivery system from polymeric nanoparticles with the incorporation of siRNA interference molecule for sema4D and demonstrate their cellular u...
Source: Journal of Bone and Mineral Research - August 4, 2014 Category: Orthopaedics Authors: Yufeng Zhang, Lingfei Wei, Richard J. Miron, Bin Shi, Zhuan Bian Tags: Original Article Source Type: research

Kirenol stimulates osteoblast differentiation through activation of the BMP and Wnt/β-catenin signaling pathways in MC3T3-E1 cells.
Abstract Kirenol has been reported to possess anti-oxidant, anti-inflammatory, anti-allergic, anti-adipogenic, and anti-arthritic activities; however, its effect on osteoblast differentiation has not yet been reported. The aim of the present study was to evaluate the effect of kirenol on osteoblast differentiation through activation of the bone morphogenetic protein (BMP) and Wnt/β-catenin signaling pathways in MC3T3-E1 cells. Kirenol markedly promoted alkaline phosphatase (ALP) activity and mineralization. Kirenol not only increased the expression of osteoblast differentiation markers, such as ALP, type I collag...
Source: Fitoterapia - July 23, 2014 Category: Biochemistry Authors: Kim MB, Song Y, Hwang JK Tags: Fitoterapia Source Type: research

IL-6-accelerated calcification by induction of ROR2 in human adipose tissue-derived mesenchymal stem cells is STAT3 dependent
Conclusion. IL-6/sIL-6R stimulation accelerated the ROR2/WNT5A pathway in hADSCs in a STAT3-dependent manner, resulting in augmented calcification. These results suggest that the mechanisms of ectopic calcification accelerated by IL-6 in hADSCs may be involved in chronic inflammatory tissues and that IL-6 inhibitors may be beneficial in the treatment of ectopic calcification in inflammatory diseases.
Source: Rheumatology - June 19, 2014 Category: Rheumatology Authors: Fukuyo, S., Yamaoka, K., Sonomoto, K., Oshita, K., Okada, Y., Saito, K., Yoshida, Y., Kanazawa, T., Minami, Y., Tanaka, Y. Tags: Myositis and Muscle Disease, Vasculitis, Spondylarthropathies, Osteoporosis and Metabolic Bone Disease, Systemic Lupus Erythematosus and Autoimmunity BASIC [AMP ] TRANSLATIONAL SCIENCE Source Type: research

Active vitamin D possesses beneficial effects on the interaction between muscle and bone.
In conclusion, these findings showed for the first time that active vitamin D plays important roles in myogenesis and muscle-induced osteoblastogenesis through OGN expression. Active vitamin D treatment may rescue the AGEs-induced sarcopenia as well as -suppressed osteoblastic differentiation via OGN expression in myoblasts. PMID: 24924628 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - June 9, 2014 Category: Biochemistry Authors: Tanaka KI, Kanazawa I, Yamaguchi T, Yano S, Kaji H, Sugimoto T Tags: Biochem Biophys Res Commun Source Type: research

Advanced Glycation End Product 3 (AGE3) Suppresses the Mineralization of Mouse Stromal ST2 Cells and Human Mesenchymal Stem Cells by Increasing TGF-β Expression and Secretion.
Abstract In diabetic patients, advanced glycation end products (AGEs) cause bone fragility because of deterioration of bone quality. We previously showed that AGEs suppressed the mineralization of mouse stromal ST2 cells. Transforming growth factor (TGF)-β is abundant in bone, and enhancement of its signal causes bone quality deterioration. However, whether TGF-β signaling is involved in the AGE-induced suppression of mineralization during the osteoblast lineage remains unknown. We therefore examined the roles of TGF-β in the AGE-induced suppression of mineralization of ST2 cells and human mesenchymal stem cell...
Source: Endocrinology - April 23, 2014 Category: Endocrinology Authors: Notsu M, Yamaguchi T, Okazaki K, Tanaka KI, Ogawa N, Kanazawa I, Sugimoto T Tags: Endocrinology Source Type: research

P53 Dependent Mitochondrial Permeability Transition Pore Opening Is Required for Dexamethasone‐Induced Death of Osteoblasts
Prolonged or overdose glucocorticoids (GCs) usage is the common cause of osteoporosis. In the present study, we studied the cellular mechanism of dexamethasone (Dex)‐induce osteoblast cell death by focusing on the role of mitochondrial permeability transition pore (mPTP). In cultured osteoblastic MC3T3‐E1 cells, Dex‐induced mPTP opening, which was demonstrated by mitochondrial membrane potential (MPP) decrease, cyclophilin‐D (CyPD)–adenine nucleotide translocator 1 (ANT‐1) mitochondrial complexation and cytochrome C (cyto‐C) release. The mPTP inhibitor sanglifehrin A (SfA) dramatically inhibited Dex‐induced...
Source: Journal of Cellular Physiology - February 25, 2014 Category: Cytology Authors: Yun‐fang Zhen, Guo‐dong Wang, Lun‐qing Zhu, Shi‐ping Tan, Fu‐yong Zhang, Xiao‐zhong Zhou, Xiao‐dong Wang Tags: Original Research Article Source Type: research