Editorial Board
Publication date: 1 December 2019Source: Molecular and Cellular Endocrinology, Volume 498Author(s): (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - October 23, 2019 Category: Endocrinology Source Type: research

Metformin alleviates oxidative stress and enhances autophagy in diabetic kidney disease via AMPK/SIRT1-FoxO1 pathway
Publication date: Available online 21 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Huiwen Ren, Ying Shao, Can Wu, Xiaoyu Ma, Chuan Lv, Qiuyue WangAbstractMetformin, as the basic pharmacological therapy and the first preventive drug in type 2 diabetes mellitus (T2DM), is proved to have potential protection in diabetic kidney disease (DKD). Here, we established a diabetic rat model induced by high-fat diet and low dose streptozotocin, and high glucose cultured rat mesangial cells (RMCs) pre-treated with metformin or transfected with AMPK, SIRT1 and FoxO1 small interfering RNA, and detected oxidative str...
Source: Molecular and Cellular Endocrinology - October 21, 2019 Category: Endocrinology Source Type: research

Type 1 diabetes affects zona pellucida and genome methylation in oocytes and granulosa cells
In this study, we generated a type 1 diabetes (T1D) mouse model by STZ injection to study the effects of T1D on zona pellucida and genomic DNA methylation of oocytes and granulosa cells. T1D mice showed fewer ovulated eggs, reduced ovarian reserve, disrupted estrus cycle, and significantly ruptured zona pellucida in 2-cell in vivo embryos compared to controls. Notably, diabetic oocytes displayed thinner zona pellucida and treatment of oocytes with high concentration glucose reduced the zona pellucida thickness. Differential methylation genes in oocytes and granulosa cells were analyzed by methylation sequencing. These gene...
Source: Molecular and Cellular Endocrinology - October 20, 2019 Category: Endocrinology Source Type: research

High-fat diet-induced dysregulation of ovarian gene expression is restored with chronic Omega-3 fatty acid supplementation
In conclusion our data suggest some benefit of DHA supplementation after HFD, particularly in regards to ovarian gene expression, however complete restoration of ovarian function was not achieved. (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - October 17, 2019 Category: Endocrinology Source Type: research

Perinatal exposure to bisphenol A (BPA) impairs neuroendocrine mechanisms regulating food intake and kisspetin system in adult male rats. Evidences of metabolic disruptor hypothesis
Publication date: Available online 10 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Cora Stoker, M. Florencia Andreoli, Laura Kass, Verónica L. Bosquiazzo, M. Florencia Rossetti, G. Canesini, Enrique H. Luque, Jorge G. RamosAbstractBisphenol A (BPA) is a compound used in the polymerization of plastic polycarbonates. It is an endocrine disruptor and it has been postulated to be an obesogen. Our objective was to determine the influence of perinatal exposure to BPA on body weight, hormone levels, metabolic parameters and hypothalamic signals that regulate food intake and kisspeptin system in adult ...
Source: Molecular and Cellular Endocrinology - October 12, 2019 Category: Endocrinology Source Type: research

Lessons from animal models of endocrine disorders caused by defects of protein folding in the secretory pathway
Publication date: Available online 9 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Yoshiaki Morishita, Peter ArvanAbstractMost peptide hormones originate from secretory protein precursors synthesized within the endoplasmic reticulum (ER). In this specialized organelle, the newly-made prohormones must fold to their native state. Completion of prohormone folding usually occurs prior to migration through the secretory pathway, as unfolded/misfolded prohormones are retained by mechanisms collectively known as ER quality control. Not only do most monomeric prohormones fold properly, but many also dimerize o...
Source: Molecular and Cellular Endocrinology - October 11, 2019 Category: Endocrinology Source Type: research

Genome-wide methylation profiling in granulosa lutein cells of women with polycystic ovary syndrome (PCOS)
Publication date: Available online 7 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): E. Makrinou, A.W. Drong, G. Christopoulos, A. Lerner, I. Chapa-Chorda, T. Karaderi, S. Lavery, K. Hardy, C.M. Lindgren, S. FranksAbstractPolycystic Ovary Syndrome (PCOS) is the most common endocrine disorder amongst women of reproductive age, whose aetiology remains unclear. To improve our understanding of the molecular mechanisms underlying the disease, we conducted a genome-wide DNA methylation profiling in granulosa lutein cells collected from 16 women suffering from PCOS, in comparison to 16 healthy controls. Samples...
Source: Molecular and Cellular Endocrinology - October 9, 2019 Category: Endocrinology Source Type: research

Involvement of CREB-regulated transcription coactivators (CRTC) in transcriptional activation of steroidogenic acute regulatory protein (Star) by ACTH
Publication date: Available online 8 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Lorna I.F. Smith, Victoria Huang, Mark Olah, Loc Trinh, Ying Liu, Georgina Hazell, Becky Conway-Campbell, Zidong Zhao, Antoine Martinez, Anne-Marie Lefrançois-Martinez, Stafford Lightman, Francesca Spiga, Greti AguileraAbstractStudies in vivo have suggested the involvement of CREB-regulated transcription coactivator (CRTC)2 on ACTH-induced transcription of the key steroidogenic protein, Steroidogenic Acute Regulatory (StAR). The present study uses two ACTH-responsive adrenocortical cell-lines, to examine the role ...
Source: Molecular and Cellular Endocrinology - October 9, 2019 Category: Endocrinology Source Type: research

Prenatally androgenized female rats develop uterine hyperplasia when adult
In this study, we aimed to investigate the impact of prenatal exposure to androgen excess on the uterus when animals reach their adulthood. We found that PH altered the morphology of the uteri that show a hyperplastic morphology with increased total uterine thickness as well as luminal epithelium thickness, with both enhanced and altered distribution of glands as compared with controls. Morphological alterations were associated with an unbalanced homeostasis as assessed by the expression of regulators of cell cycle progression and cell death dynamics. PH also causes disturbances in the cell cycle of the uterine tissue and ...
Source: Molecular and Cellular Endocrinology - October 5, 2019 Category: Endocrinology Source Type: research

Chaperones, somatotroph tumors and the cyclic AMP (cAMP)-dependent protein kinase (PKA) pathway
Publication date: Available online 3 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Marie Helene Schernthaner-Reiter, Giampaolo Trivellin, Constantine A. StratakisAbstractThe cAMP-PKA pathway plays an essential role in the pituitary gland, governing cell differentiation and survival, and maintenance of endocrine function. Somatotroph growth hormone transcription and release as well as cell proliferation are regulated by the cAMP-PKA pathway; cAMP-PKA pathway abnormalities are frequently detected in sporadic as well as in hereditary somatotroph tumors and more rarely in other pituitary tumors. Inactivati...
Source: Molecular and Cellular Endocrinology - October 4, 2019 Category: Endocrinology Source Type: research

Homeostasis disrupted: eating disorders as a paradigm of psychosomatic disorders
Publication date: Available online 3 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Janet Treasure, Palmiero Monteleone, Fernando Fernandez-Aranda (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - October 4, 2019 Category: Endocrinology Source Type: research

Upregulation of adamts9 by gonadotropin in preovulatory follicles of zebrafish
Publication date: Available online 2 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Dong Teng Liu, Wan Shu Hong, Shi Xi Chen, Yong ZhuAbstractPreviously we had identified adamts9 as a downstream target of Pgr, which is essential for ovulation in zebrafish. The primary goal of this study is to determine whether human chorionic gonadotropin (hCG, LH analog) also regulate adamts9 expression prior to ovulation. The expression of adamts9 was induced by hCG in a dose and time dependent manner in zebrafish preovulatory follicles in vitro. Interestingly, the stimulatory effect of hCG on adamts9 expression was n...
Source: Molecular and Cellular Endocrinology - October 3, 2019 Category: Endocrinology Source Type: research

The challenging use and interpretation of blood biomarkers of exposure related to lipophilic endocrine disrupting chemicals in environmental health studies
Publication date: Available online 1 October 2019Source: Molecular and Cellular EndocrinologyAuthor(s): German Cano-Sancho, Philippe Marchand, Bruno Le Bizec, Jean-Philippe AntignacAbstractThe use of exposure biomarkers has been growing during the last decades, being considered the ‘gold-standard’ approach for individual exposure assessment to environmental chemicals. However, lipophilic endocrine disrupting chemicals (LEDC) have specific physicochemical and biological properties implying particular analytical challenges and interpretative caveats. The epidemiological literature is therefore afflicted by method...
Source: Molecular and Cellular Endocrinology - October 2, 2019 Category: Endocrinology Source Type: research

Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity
Publication date: Available online 30 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Davide Gnocchi, Ewa C.S. Ellis, Helene Johansson, Mats Eriksson, Giovannella Bruscalupi, Knut R. Steffensen, Paolo PariniAbstractUntil three decades, ago 3,5-diiodothyronine (3,5-T2) and 3,3′-diiodothyronine (3,3′-T2) were considered products of thyroid hormone catabolism without biological activity. Some metabolic effects have been described in rodents, but the physiological relevance in humans and the mechanisms of action are unknown. Aim of this work was to investigate the role and the mechanisms of act...
Source: Molecular and Cellular Endocrinology - October 2, 2019 Category: Endocrinology Source Type: research

Generation of a lentiviral vector system to efficiently express bioactive recombinant human prolactin hormones
Publication date: Available online 30 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Hernán Alarcón, Elena Bonzon-Kulichenko, Rocío Peinado, Filip Lim, Jesús Vázquez, Antonio RodríguezAbstractThe contribution of the pleiotropic hormone Prolactin (PRL) to several physiological and pathological processes is still unknown. To clarify the role of PRL in these processes during the last decade, different human PRL antagonists have been produced to either partially or fully block the wild type hormone activity. In this work, we have cloned these wild type and antagon...
Source: Molecular and Cellular Endocrinology - October 1, 2019 Category: Endocrinology Source Type: research

miR-181a promotes porcine granulosa cell apoptosis by targeting TGFBR1 via the activin signaling pathway
Publication date: Available online 28 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Jia-Qing Zhang, Bin-Wen Gao, Hong-Xia Guo, Qiao-Ling Ren, Xian-Wei Wang, Jun-Feng Chen, Jing Wang, Zi-Jing Zhang, Qiang Ma, Bao-Song XingAbstractActivin/Smad3 signaling plays a pivotal role in follicle development and atresia. However, the precise mechanisms underlying this process are not yet fully understood. Herein, we identified miR-181a as a central component of activin/Smad3-mediated follicle atresia. miR-181a was strikingly upregulated in porcine atretic follicles, which induced the apoptosis of porcine granulo...
Source: Molecular and Cellular Endocrinology - September 29, 2019 Category: Endocrinology Source Type: research

Targeting the pentose phosphate pathway increases reactive oxygen species and induces apoptosis in thyroid cancer cells
Publication date: Available online 26 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Chien-Liang Liu, Yi-Chiung Hsu, Jie-Jen Lee, Ming-Jen Chen, Chi-Hsin Lin, Shih-Yuan Huang, Shih-Ping ChengAbstractThe pentose phosphate pathway (PPP) plays an important role in the biosynthesis of ribonucleotide precursor and NADPH. Cancer cells frequently increase the flux of glucose into the PPP to support the anabolic demands and regulate oxidative stress. Consistently, metabolomic analyses indicate an upregulation of the PPP in thyroid cancer. In the present study, we found that the combination of glucose-6-phosph...
Source: Molecular and Cellular Endocrinology - September 28, 2019 Category: Endocrinology Source Type: research

Amh regulate female folliculogenesis and fertility in a dose-dependent manner through Amhr2 in Nile tilapia
Publication date: Available online 24 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Xingyong Liu, Hesheng Xiao, Mimi Jie, Shengfei Dai, Xin Wu, Minghui Li, Deshou WangAbstractIn the present study, Amh was found to be abundantly expressed in the granulosa cells of the primary growth follicles, and Amhr2 in the granulosa cells, oogonia and phase I oocytes in tilapia by immunohistochemistry. In addition, Amh and Amhr2 were also found to be expressed in the brain and pituitary. Heterozygous mutation of either amh or amhr2 resulted in increased primary growth follicles and decreased fertility, and homozyg...
Source: Molecular and Cellular Endocrinology - September 25, 2019 Category: Endocrinology Source Type: research

MALAT1 is involved in the pathophysiological process of PCOS by modulating TGFβ signaling in granulosa cells
In conclusion, MALAT1 reduction was identified in GCs, which may contribute to the pathophysiological processes of PCOS by regulating TGFβ signaling through sponging miR-125b and miR-203a. (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - September 24, 2019 Category: Endocrinology Source Type: research

Increased cytokines production and oxidative stress are related with purinergic signaling and cell survival in post-thyroidectomy hypothyroidism
This study demonstrates that the hypothyroidism is related to changes in purinergic system, increased cytokines production and oxidative stress, which interfere in the cell life and signaling. (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - September 24, 2019 Category: Endocrinology Source Type: research

Adenoviral transduction of EGFR into pregnancy-adapted uterine artery endothelial cells remaps growth factor induction of endothelial dysfunction
Publication date: Available online 21 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Luca Clemente, Derek S. Boeldt, Mary A. Grummer, Mayu Morita, Terry K. Morgan, Greg J. Wiepz, Paul J. Bertics, Ian M. BirdAbstractDuring pregnancy, uterine vascular vasodilation is enhanced through adapted Ca2+ signaling, facilitated through increased endothelial connexin 43 (Cx43) gap junctional communication (GJC). In preeclampsia (PE), this adaptive response is missing. Of note, the angiogenic factor VEGF can also act via Src and ERK to close Cx43 gap junctions. While VEGFR2 is necessary for such closure, a role VE...
Source: Molecular and Cellular Endocrinology - September 22, 2019 Category: Endocrinology Source Type: research

Orphan G-protein coupled receptor 183 (GPR183) potentiates insulin secretion and prevents glucotoxicity-induced β-cell dysfunction
In conclusion, the beneficial impact of GPR183 activation on β-cell function makes it a potential therapeutic target to prevent or reverse β-cell dysfunction. (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - September 22, 2019 Category: Endocrinology Source Type: research

Metformin has a direct effect on ovarian cells that is dependent on organic cation transporters
Publication date: Available online 20 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Mariana Di Pietro, Candela Velazquez, María Eugenia Matzkin, Mónica Beatriz Frungieri, Mariana Gómez Peña, Ignacio de Zúñiga, Natalia Pascuali, Griselda Irusta, María Silvia Bianchi, Fernanda Parborell, Dalhia AbramovichAbstractMetformin (MET) is the most widely prescribed hypoglycemic drug in type 2 diabetes and Polycystic Ovary Syndrome. Besides its effects on glucose metabolism, MET exerts beneficial effects on these patients’ fertility. However, the exact mech...
Source: Molecular and Cellular Endocrinology - September 22, 2019 Category: Endocrinology Source Type: research

Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation
Publication date: Available online 17 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): C. Fraichard, F. Bonnet, A. Garnier, M. Hébert-Schuster, A. Bouzerara, P. Gerbaud, I. Ferecatu, T. Fournier, I. Hernandez, S. Trabado, J. GuibourdencheAbstractPlacental syncytiotrophoblast (ST) is considered as the main placental endocrine tissue secreting progesterone, a steroid essential for maintenance of pregnancy. However, each step of progestins production has been poorly investigated in villous cytotrophoblast (VCT) regarding ST formation. We aimed to characterize progestins production during human diffe...
Source: Molecular and Cellular Endocrinology - September 19, 2019 Category: Endocrinology Source Type: research

Mice lacking angiotensin type 2 receptor exhibit a sex-specific attenuation of insulin sensitivity
In this study we further explored the role of the AT2R on insulin action and glucose homeostasis by investigating the glycemic profile and in vivo insulin signaling status in insulin-target tissues from both male and female AT2R knockout (KO) mice. When compared to the respective wild-type (WT) group, glycemia and insulinemia was unaltered in AT2RKO mice regardless of sex. However, female AT2RKO mice displayed decreased insulin sensitivity compared to their WT littermates. This was accompanied by a compensatory increase in adiponectinemia and with a specific attenuation of the activity of main insulin signaling components ...
Source: Molecular and Cellular Endocrinology - September 19, 2019 Category: Endocrinology Source Type: research

A long-acting, dual-agonist analogue of lamprey GLP-1 shows potent insulinotropic, β-cell protective, and anorexic activities and improves glucose homeostasis in high fat-fed mice
Publication date: Available online 17 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Galyna V. Graham, Andrew McCloskey, Yasser H. Abdel-Wahab, J. Michael Conlon, Peter R. FlattAbstractPeptidase-resistant analogues of GLP-1 peptides from sea lamprey and paddlefish ([D-Ala2]palmitoyl-lamprey GLP-1 and [D-Ala2]palmitoyl-paddlefish GLP-1) produced significant (P ≤ 0.05) and concentration-dependent increases in insulin release from BRIN-BD11 clonal β-cells and from isolated mouse islets. Both analogues retained the ability of the native peptides to activate both the GLP-1 receptor (GLP1R) and ...
Source: Molecular and Cellular Endocrinology - September 19, 2019 Category: Endocrinology Source Type: research

Postnatal knockout of beta cell insulin receptor impaired insulin secretion in male mice exposed to high-fat diet stress
Publication date: Available online 18 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Amanda Oakie, Liangyi Zhou, Sydney Rivers, Christy Cheung, Jinming Li, Rennian WangAbstractThe presence of insulin receptor (IR) on insulin-secreting beta cells suggests an autocrine regulatory role for insulin in its own signalling. Congenital beta cell-specific IR knockout (βIRKO) mouse studies have demonstrated the development of age-dependent glucose intolerance. We investigated the role of beta cell IR signalling specifically during postnatal life following undisturbed prenatal pancreatic development and mat...
Source: Molecular and Cellular Endocrinology - September 19, 2019 Category: Endocrinology Source Type: research

Evaluating the effects on steroidogenesis of estragole and trans-anethole in a feto-placental co-culture model
In this study, we determined whether estragole and its isomer trans-anethole interfered with feto-placental steroidogenesis in a human co-culture model composed of fetal-like adrenocortical (H295R) and placental trophoblast-like (BeWo) cells. Estragole and trans-anethole are considered the biologically active compounds within basil and fennel seed essential oils, respectively. After a 24 h exposure of the co-culture to 2.5, 5.2 and 25 μM estragole or trans-anethole, hormone concentrations of estradiol, estrone, dehydroepiandrosterone, androstenedione, progesterone and estriol were significantly increased. Using RT-q...
Source: Molecular and Cellular Endocrinology - September 17, 2019 Category: Endocrinology Source Type: research

In vitro cytotoxicity of cabazitaxel in adrenocortical carcinoma cell lines and human adrenocortical carcinoma primary cell cultures
Publication date: Available online 16 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Martina Fragni, Lilian Patricia Palma Lopez, Elisa Rossini, Andrea Abate, Deborah Cosentini, Valentina Salvi, Sara Vezzoli, Pietro Luigi Poliani, Daniela Bosisio, Constanze Hantel, Guido A.M. Tiberio, Salvatore Grisanti, Maurizio Memo, Massimo Terzolo, Alfredo Berruti, Sandra SigalaAbstractAdrenocortical cancer (ACC) is a rare and aggressive malignancy with a poor prognosis. The overall 5-year survival rate of patients with ENS@T stage IV ACC is less than 15%. Systemic antineoplastic therapies have a limited efficacy ...
Source: Molecular and Cellular Endocrinology - September 17, 2019 Category: Endocrinology Source Type: research

The effects of acute BPA exposure on skeletal muscle mitochondrial function and glucose metabolism
In this study, we investigated the effects of BPA on skeletal muscle mitochondrial function and insulin sensitivity. L6 myotubes were treated with BPA (1 nM–105 nM) during the last 24 h of differentiation. Following exposure to 105 nM of BPA, resting and maximal oxygen consumption rates were decreased, whereas mitochondrial proton leak was increased. Overall metabolic activity, measured by redox ability, was decreased in L6 myotubes exposed to 105 nM of BPA. At this concentration, insulin-stimulated glucose uptake was increased, which corresponded to an increased phosphorylation of the insulin signaling pro...
Source: Molecular and Cellular Endocrinology - September 17, 2019 Category: Endocrinology Source Type: research

Evolution of the androgen receptor: Perspectives from human health to dancing birds
Publication date: Available online 13 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Eric R. Schuppe, Meredith C. Miles, Matthew J. FuxjagerAbstractAndrogenic hormones orchestrate the development and activation of diverse reproductive phenotypes across vertebrates. Although extensive work investigates how selection for these traits modifies individual elements of this signaling system (e.g., hormone or androgen receptor [AR] levels), we know less about natural variation in the AR sequence across vertebrates. Our knowledge of AR sequence mutations is largely limited to work in human patients or cell-li...
Source: Molecular and Cellular Endocrinology - September 14, 2019 Category: Endocrinology Source Type: research

Role of estrone on the regulation of osteoblastogenesis
Publication date: Available online 13 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): M. Carla Crescitelli, M. Belén Rauschemberger, Sabrina Cepeda, Marisa Sandoval, Virginia L. MassheimerAbstractAlthough estradiol bone contribution has been deeply studied, little is known about the action of estrone. We investigated the direct action of estrone on osteoblasts growth and differentiation, with focus on the biochemical mechanism displayed by the estrogen. Murine calvarial osteoblast cultures in vitro exposed to 10 nM estrone were employed. Estrone enhanced gene expression of the osteogenic diffe...
Source: Molecular and Cellular Endocrinology - September 14, 2019 Category: Endocrinology Source Type: research

Neural and endocrine mechanisms underlying stress-induced suppression of pulsatile LH secretion
Publication date: Available online 12 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Richard B. McCosh, Kellie M. Breen, Alexander S. KauffmanAbstractStress is well-known to inhibit a variety of reproductive processes, including the suppression of episodic Gonadotropin releasing hormone (GnRH) secretion, typically measured via downstream luteinizing hormone (LH) secretion. Since pulsatile secretion of GnRH and LH are necessary for proper reproductive function in both males and females, and stress is common for both human and animals, understanding the fundamental mechanisms by which stress impairs LH ...
Source: Molecular and Cellular Endocrinology - September 14, 2019 Category: Endocrinology Source Type: research

Which chemicals should be grouped together for mixture risk assessments of male reproductive disorders?
Publication date: Available online 13 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Andreas KortenkampAbstractThere is concern about cumulative exposures to compounds that disrupt male sexual differentiation in foetal life, leading to irreversible effects in adulthood, including declines in semen quality, testes non-descent, malformations of the penis and testis cancer. Traditional chemical-by-chemical risk assessment approaches cannot capture the likely cumulative health risks. Past efforts of focusing on combinations of phthalates, a subgroup of chemicals suspected of contributing to these risks, d...
Source: Molecular and Cellular Endocrinology - September 14, 2019 Category: Endocrinology Source Type: research

Angiotensin II type 2 receptor mediates high fat diet-induced cardiomyocyte hypertrophy and hypercholesterolemia
Publication date: Available online 11 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Vanessa M. Lima, Caroline A. Lino, Nathalia Senger, Tábatha de Oliveira Silva, Renata I.B. Fonseca, Michael Bader, Robson A.S. Santos, Jose Donato Júnior, Maria Luiza M. Barreto-Chaves, Gabriela P. DinizAbstractObesity is the major risk factor for several cardiovascular and metabolic disorders. Previous studies reported that deletion of Angiotensin II type 2 receptor (AT2R) protects against metabolic dysfunctions induced by high fat (HF) diet. However, the role of AT2R in obesity-induced cardiac hypertro...
Source: Molecular and Cellular Endocrinology - September 12, 2019 Category: Endocrinology Source Type: research

Mechanism of pulsatile GnRH release in primates: Unresolved questions
This article reviews some old works and discusses unresolved issues on the mechanism of GnRH pulse generation. (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - September 11, 2019 Category: Endocrinology Source Type: research

Rhythmic growth hormone secretion in physiological and pathological conditions: Lessons from rodent studies
Publication date: Available online 6 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Lili Huang, Zhengxiang Huang, Chen ChenAbstractEvolutionally conserved in all mammalians, the release of GH occurs in a rhythmic pattern, characterized by several dominant surges (pulsatile GH) with tonic low inter-pulse levels (tonic GH). Such pulsatile secretion pattern is essential for many physiological actions of GH on different tissues with defined gender dimorphism. Rhythmic release of pulsatile GH is tightly controlled by hypothalamic neurons as well as peripheral metabolic factors. Changes of GH pattern occurs...
Source: Molecular and Cellular Endocrinology - September 8, 2019 Category: Endocrinology Source Type: research

Development of a novel fluorescent ligand of growth hormone secretagogue receptor based on the N-Terminal Leap2 region
Publication date: Available online 6 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Franco Barrile, Céline M'Kadmi, Pablo N. De Francesco, Agustina Cabral, Guadalupe García Romero, Emilio R. Mustafá, Sonia Cantel, Marjorie Damian, Sophie Mary, Séverine Denoyelle, Jean-Louis Banères, Jacky Marie, Jesica Raingo, Jean-Alain Fehrentz, Mario PerellóAbstractLiver-expressed antimicrobial peptide 2 (LEAP2) was recently recognized as an endogenous ligand for the growth hormone secretagogue receptor (GHSR), which also is a receptor for the hormone ghrelin. LEAP2 blocks ...
Source: Molecular and Cellular Endocrinology - September 8, 2019 Category: Endocrinology Source Type: research

Growth hormone/STAT5 signaling in proopiomelanocortin neurons regulates glucoprivic hyperphagia
Publication date: Available online 5 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Paula G.F. Quaresma, Pryscila D.S. Teixeira, Isadora C. Furigo, Frederick Wasinski, Gisele C. Couto, Renata Frazão, Edward O. List, John J. Kopchick, Jose DonatoAbstractSeveral hypothalamic neuronal populations are directly responsive to growth hormone (GH) and central GH action regulates glucose and energy homeostasis. However, the potential role of GH signaling in proopiomelanocortin (POMC) neurons has not been studied yet. Thus, we investigated whether POMC neurons are responsive to GH and if ablation of GH r...
Source: Molecular and Cellular Endocrinology - September 6, 2019 Category: Endocrinology Source Type: research

Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells
Publication date: Available online 4 September 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Breanne C. Morrell, Lingna Zhang, Luis F. Schütz, M. Chiara Perego, Excel Rio S. Maylem, Leon J. SpicerAbstractOverexpression of the transcription factor, E2F8, has been associated with ovarian cancer. Objectives of this study were to determine: 1) if E2F8 gene expression in granulosa cells (GC) and theca cells (TC) change with follicular development, and 2) if E2F8 mRNA abundance in TC and GC is hormonally regulated. Using real-time PCR, E2F8 mRNA abundance in GC and TC was greater (P 
Source: Molecular and Cellular Endocrinology - September 5, 2019 Category: Endocrinology Source Type: research

Editorial Board
Publication date: 1 October 2019Source: Molecular and Cellular Endocrinology, Volume 496Author(s): (Source: Molecular and Cellular Endocrinology)
Source: Molecular and Cellular Endocrinology - August 29, 2019 Category: Endocrinology Source Type: research

Pathological pulses in PCOS
Publication date: Available online 25 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Christopher Coyle, Rebecca E. CampbellAbstractPolycystic ovary syndrome (PCOS) is a highly prevalent endocrine disorder associated with hyperandrogenism and anovulation. Although a spectrum disorder, many women with PCOS exhibit elevated luteinizing hormone (LH) pulse frequency and an elevated LH to follicle stimulating hormone ratio. This aberrant pattern of gonadotrophin signalling drives many of the downstream ovarian features of PCOS, including increased androgen synthesis, and indicates neuroendocrine impairments up...
Source: Molecular and Cellular Endocrinology - August 26, 2019 Category: Endocrinology Source Type: research

A new perspective of lactatogenesis by isolated adipocytes
Publication date: Available online 20 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Filipy Borghi, Camila L. Morais, Carolina Silva, Priscila C. da Silva, Larissa Y. Ishizu, Gustavo T. Costa, Dora M. Grassi-KassisseAbstractIncreased adipose tissue mass exhibited greater capacity of glucose transformation in lactate, highlighting lactatogenesis as a crucial factor in body size. Classically, lactate produced by isolated adipocytes are expressed per million of cells and were never correlated with their size. Spontaneously hypertensive rats (SHR) have a lower body weight and smaller adipocytes when compared...
Source: Molecular and Cellular Endocrinology - August 21, 2019 Category: Endocrinology Source Type: research

Endoplasmic reticulum quality control in lipoprotein metabolism
Publication date: Available online 20 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Cari M. Koerner, Benjamin S. Roberts, Saskia B. NeherAbstractLipids play a critical role in energy metabolism, and a suite of proteins is required to deliver lipids to tissues. Several of these proteins require an intricate endoplasmic reticulum (ER) quality control (QC) system and unique secondary chaperones for folding. Key examples include apolipoprotein B (apoB), which is the primary scaffold for many lipoproteins, dimeric lipases, which hydrolyze triglycerides from circulating lipoproteins, and the low-density lipop...
Source: Molecular and Cellular Endocrinology - August 21, 2019 Category: Endocrinology Source Type: research

Cre/lox generation of a novel whole-body Kiss1r KO mouse line recapitulates a hypogonadal, obese, and metabolically-impaired phenotype
Publication date: Available online 20 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Kristen P. Tolson, Nuha Marooki, Andrew Wolfe, Jeremy T. Smith, Alexander S. KauffmanAbstractKisspeptin and its receptor, Kiss1r, act in centrally to stimulate reproduction. Recent evidence indicates that kisspeptin is also important for body weight and metabolism, as whole-body Kiss1r KO mice, developed with gene trap technology, display obesity and reduced metabolism. Kiss1r is expressed in brain and multiple peripheral tissues, but it is unknown which is responsible for the metabolic phenotype. Here, we sought to conf...
Source: Molecular and Cellular Endocrinology - August 20, 2019 Category: Endocrinology Source Type: research

Bone morphogenetic protein 6 affects cell-cell communication by altering the expression of Connexin43 in human granulosa-lutein cells
In this study, we demonstrate that BMP6 treatment significantly suppressed the expression of Cx43 in both primary and immortalized (SVOG) human granulosa-lutein cells. Using both pharmacological inhibitors and small interfering RNA-mediated knockdown approaches, we demonstrate that ALK2 and ALK3 BMP type I receptors are involved in BMP6-induced suppressive effects on Cx43 expression and GJIC activity in SVOG cells. Furthermore, these cellular activities are most likely mediated by the SMAD1/SMAD5-SMAD4-dependent signaling pathway. Notably, the ChIP analyses demonstrated that phosphorylated SMADs could bind to human Cx43 pr...
Source: Molecular and Cellular Endocrinology - August 19, 2019 Category: Endocrinology Source Type: research

Cafeteria diet induces progressive changes in hypothalamic mechanisms involved in food intake control at different feeding periods in female rats
Publication date: Available online 17 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Lazzarino Gisela Paola, Acutain María Florencia, Canesini Guillermina, Andreoli María Florencia, Ramos Jorge GuillermoAbstractWe studied the effects of cafeteria diet (CAF) intake from weaning on mRNA levels and DNA methylation state of feeding-related neuropeptides and hormone receptors in individual hypothalamic nuclei at different feeding periods. Four weeks of CAF (short-term) increased energy intake and adiposity, without affecting neuropeptides’ expression. Eleven weeks of CAF (medium-term) incr...
Source: Molecular and Cellular Endocrinology - August 18, 2019 Category: Endocrinology Source Type: research

Acute heat-treatment disrupts inhibin-related protein production and gene expression in the adult rat testis
Publication date: Available online 15 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Rashid A. Aldahhan, Peter G. Stanton, Helen Ludlow, David M. de Kretser, Mark P. HedgerAbstractHeat reversibly disrupts spermatogenesis, but the effects on Sertoli cell (SC) function and inhibin/activin-related proteins are less well-defined. Adult rat testis weights decreased by 40% within 2 weeks after heat-treatment (43 °C, 15 min), due to loss of pachytene spermatocytes and round spermatids. Coincident effects were reduced SC nuclear volume at one week and>50% reduction in expression of several critical SC...
Source: Molecular and Cellular Endocrinology - August 17, 2019 Category: Endocrinology Source Type: research

β-adrenergic receptors in the up-regulation of COX2 expression and prostaglandin production in testicular macrophages: Possible relevance to male idiopathic infertility
Publication date: Available online 16 August 2019Source: Molecular and Cellular EndocrinologyAuthor(s): María Eugenia Matzkin, Eugenia Riviere, Soledad Paola Rossi, Roberto Ponzio, Elisa Puigdomenech, Oscar Levalle, Claudio Terradas, Ricardo Saúl Calandra, Artur Mayerhofer, Mónica Beatriz FrungieriAbstractaCatecholaminergic neuronal elements (CNE) and macrophages (MACs) are increased in testes of patients with idiopathic infertility. Now, we describe an anatomical proximity between CNE and MACs, expression of specific α- and β-adrenergic receptors (ADRs) subtypes in MACs, and a positive corr...
Source: Molecular and Cellular Endocrinology - August 17, 2019 Category: Endocrinology Source Type: research

MiR-29a regulates the proliferation, aromatase expression, and estradiol biosynthesis of human granulosa cells in polycystic ovary syndrome
This study aimed to reveal the role of miR-29a in PCOS. MiR-29a levels were measured in the granulosa cells (GCs) of forty-seven PCOS patients and forty-seven controls. A receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic value of miR-29a in non-hyperandrogenism PCOS. MiR-29a was overexpressed in KGN and COV434 cells to examine its roles in proliferation, cell-cycle progression, and steroidogenesis. MiR-29a was significantly down-regulated in PCOS patients, and associated with an increased antral follicle count. The ROC curve showed a major separation between PCOS patients and controls. Mi...
Source: Molecular and Cellular Endocrinology - August 15, 2019 Category: Endocrinology Source Type: research