Inhibition of miR ‐124 improves neonatal necrotizing enterocolitis via an MYPT1 and TLR9 signal regulation mechanism
ConclusionmiR ‐124 was a promoter of NEC, which promotes the intestine cell apoptosis and inflammatory cell infiltration through the inhibition of TLR9 expression by targeting ROCK1. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yiyu Yin, Zhenfang Qin, Xiaobing Xu, Xu Liu, Huaxin Zou, Xiaole Wu, Junhua Cao Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

MicroRNA ‐99b suppresses human cervical cancer cell activity by inhibiting the PI3K/AKT/mTOR signaling pathway
This study aimed to explore the effect of miR ‐99b‐5p (miR‐99b) on invasion and migration in cervical cancer through the phosphatidylinositol 3‐kinase (PI3K)/protein kinase B (AKT)/mechanistic target of rapamycin (mTOR) signaling pathway. The microarray‐based analysis was used to screen out differentially expressed miRNAs. Expression of miR‐99b, PI3K, AKT, mTOR, and ribosomal protein S6 kinase (p70S6K) was determined in both cervical cancer tissues and paracancerous tissues. Next, alteration of miR‐99b expression in cervical cancer was conducted to evaluate levels of PI3K, AKT, mTOR, p70S6K matrix metallopept...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yong ‐Jie Li, Yue Wang, Yi‐Ying Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Altered plasma proteins released from platelets and endothelial cells are associated with human patent ductus arteriosus
Ductus arteriosus closure involves functional and structural remodeling, controlled by many factors and we for the first time demonstrate six key differential plasma proteins in human patent ductus arteriosus patients using proteomic technology and present a model to illustrate the constriction and closure of ductus arteriosus. Those proteins are closely related to platelet activation and coagulation cascades, complement mannan ‐binding‐lectin, and other systemic signaling pathways. Our findings for the first time indicate that the differential proteins involved in different pathways may play key roles in the nonclosur...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Hai ‐Tao Hou, Xi‐Zhang, Jun Wang, Li‐Xin Liu, Jian‐Feng Zhang, Qin Yang, Guo‐Wei He Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The possible role of bacteria, viruses, and parasites in initiation and exacerbation of irritable bowel syndrome
Irritable bowel syndrome (IBS) is a prolonged and disabling functional gastrointestinal disorder with the incidence rate of 18% in the world. The pathophysiology of the IBS is hardly understood, whereas several possible mechanisms, such as visceral hypersensitivity, irregular gut motility, abnormal brain –gut relations, and the role of infectious agents, are implicated in initiation and development this syndrome. It appears that the main obligatory pathogens correspond with the IBS disease. AbstractIrritable bowel syndrome (IBS) is a prolonged and disabling functional gastrointestinal disorder with the incidence rate...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Aref Shariati, Fateme Fallah, Ali Pormohammad, Ali Taghipour, Hossein Safari, Alireza salami chirani, Sahar Sabour, Mahmood Alizadeh ‐Sani, Taher Azimi Tags: REVIEW ARTICLE Source Type: research

Cell fate regulation by reticulon ‐4 in human prostate cancers
Our results showed that reticulon ‐4 (RTN4) was downregulated and targeted by miR‐148a‐3p, and inhibited prostate cancer cell proliferation by inducing cell cycle arrest and senescence, suggesting that RTN4 may be an important molecular event linked to prostate cancer. AbstractReticulon ‐4 (RTN4), a reticulon family protein localized in the endoplasmic reticulum, is reported to be involved in multiple physiological processes like neuroendocrine secretion and membrane trafficking in neuroendocrine cells. Previous studies have presented a great potential of RTN4 for the treatment o f autoimmune‐mediated demyelinati...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Hu Zhao, Weipeng Su, Changyan Zhu, Tengyue Zeng, Shunliang Yang, Weizhen Wu, Dong Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Effects of nebulized N ­‐acetylcystein on the expression of HMGB1 and RAGE in rats with hyperoxia‐­induced lung injury
High mobility group box 1 (HMGB1) and receptor for advanced glycation end product (RAGE) were involved in the pathogenesis of hyperoxia ‐induced lung injury, inhalation ofN‐acetylcysteine might alleviate hyperoxia‐induced lung injury by regulating the expression of HMGB1 and RAGE. AbstractObjectiveTo investigate the role of high mobility group box 1 (HMGB1) and receptor for advanced glycation end product (RAGE) in the lungs of hyperoxia ‐induced rats and the effect ofN‐­acetlycystein (NAC).MethodsA model of hyperoxic lung injury was established, rats in the NAC intervention, and control, hyperoxia group were ...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Junying Qiao, Lixia Chen, Xianjie Huang, Feifei Guo Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Cholinergic activity regulates the secretome of epicardial adipose tissue: Association with atrial fibrillation
Muscarinic receptor type 3 is upregulated after adipogenesis.Epicardial fat from atrium or ventricles express this receptor and contain cholinergic activity.Epicardial fat ‐released proteins after cholinergic activity as possible predictors of postoperative AF AbstractBotulinum toxin injection on epicardial fat, which inhibits acetylcholine (ACh) release, reduced the presence of atrial fibrillation (AF) in patients after heart surgery. Thus, we wanted to study the profile of the released proteins of epicardial adipose tissue (EAT) under cholinergic activity (ACh treatment) and their value as AF predictors. Biopsies, expl...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Marinela Couselo ‐Seijas, Jose N. López‐Canoa, Rosa M. Agra‐Bermejo, Esther Díaz‐Rodriguez, Angel L. Fernandez, Jose M. Martinez‐Cereijo, Dario Durán‐Muñoz, Susana B. Bravo, Alba Velo, Laila González‐Melchor, Xesus A. Fernánd Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Knockdown of NEAT1 repressed the malignant progression of glioma through sponging miR ‐107 and inhibiting CDK14
We found that inhibition of nuclear paraspeckle assembly transcript 1 (NEAT1) repressed glioma development via sponging miR ‐107. The correlation between NEAT1 and miR‐107 was validated in our study, and cyclin dependent kinase 14 (CDK14) was targeted by miR‐107 by using bioinformatics analysis. All these data revealed that NEAT1/miR‐107/CDK14 axis was involved in glioma development AbstractAberrant expressions of long noncoding RNAs (lncRNAs) contribute to carcinogenesis via regulating tumor suppressors or oncogenes. LncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) has been recognized as an oncogene to pro...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yingwei Zhen, Yang Nan, Shewei Guo, Longzhou Zhang, Ge Li, Saichao Yue, Xianzhi Liu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

c ‐Met inhibition is required for the celecoxib‐attenuated stemness property of human colorectal cancer cells
In this study, we found that among these NSAIDs, celecoxib has t he most potency for CSC inhibition of CRC cells, largely correlating to inhibition of c‐Met, not COX‐2. Further analysis reveals that c‐Met activity was required for basal CSC property. Silence of c‐Met blocked whereas overexpression of c‐Met enhanced the celecoxib‐inhibited CSC propert y. Collectively, these results not only first elucidate the mechanism underlying celecoxib‐inhibited CSC but also indicate c‐Met as a critical factor for the CSC property of CRC cells. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yueh ‐Ming Lin, Chien‐Chang Lu, Yi‐Ping Hsiang, Shu‐Chuan Pi, Chih‐I Chen, Kung‐Chuan Cheng, Hsiao‐Lin Pan, Pei‐Hsuan Chien, Yun‐Ju Chen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Preparation of adriamycin gelatin microsphere ‐loaded decellularized periosteum that is cytotoxic to human osteosarcoma cells
The effectiveness of these two scaffolds in killing human osteosarcoma cells was tested by evaluating cell viability overtime of the cancer cells cultured with the scaffolds. In summary, a gelatin/decellularized periosteum ‐based biologic scaffold material was designed aiming for local delivery of chemotherapy drugs for osteosarcoma, with the results showing ability of the scaffolds in sustaining release of the cancer drug and in suppressing growth of the cancer cells in vitro AbstractThe purpose of this study was to develop a novel approach to treat bone osteosarcoma using a multipurpose scaffold aiming for local drug d...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Chuan Chen, Jianghui Dong, Hong Chen, Xin Wang, Jin Mei, Liping Wang, Cory J Xian Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Redefining the signaling pathways from pluripotency to pancreas development: In vitro β‐cell differentiation
We summarized the current knowledge on the developmental biology of the pancreas to understand the mechanism underlying the insulin ‐producing cell (IPC) protocols, and discussed the appropriate signaling involved in the early body axes and endoderm patterning up to endocrine precursor (EP) and β‐cell specification. In addition, we showed that transplantation of differentiated embryonic stem (dES) cells in early differentia tion stage, leads to a better outcome, compared with full differentiation of mature β‐cells’ graft. AbstractPancreatic β‐cells are destroyed by the immune system, in type 1 ...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Mahmoud Hashemitabar, Elham Heidari Tags: MINI ‐REVIEW Source Type: research

Embryo quality, and not chromosome nondiploidy, affects mitochondrial DNA content in mouse blastocysts
This study constructed haploid, diploid, triploid, and tetraploid blastocysts by parthenogenetic activation, intracytoplasmic sperm injection with one or two sperm heads, blastomere electrofusion, respectively, and generated reduced cytoplasm quality embryos from diabetic mouse and in vitro fertilization of aged oocytes, and examined whether nondiploidy or reduced cytoplasm quality causes premature mitochondrial biosynthesis. MtDNA numbers of each blastocyst from different models were tested by absolute quantitative real ‐time polymerase chain reaction. It was found that mtDNA content in preimplantation embryos was not a...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Ying Jing, Li Li, Yuan ‐Yuan Li, Ying‐Chun Ouyang, Qing‐Yuan Sun, Cui‐Lian Zhang, Rong Li Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

miR ‐142‐3p as tumor suppressor miRNA in the regulation of tumorigenicity, invasion and migration of human breast cancer by targeting Bach‐1 expression
ConclusionFor the first time, our results revealed that miR ‐142‐3p could target Bach‐1in breast cancer cells leading to the reduction of EMT‐related proteins and reduced cell proliferation, invasion, and migration. The results also demonstrated that miR‐142‐3p could regulate important tumor suppressor miRNAs in breast cancer cells. In conclusion , our results suggest that miR‐142‐3p could be a good candidate for the targeted therapy of breast cancer, especially for the invasive type. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Behzad Mansoori, Ali Mohammadi, Mehri Ghasabi, Solmaz Shirjang, Razeieh Dehghan, Vahid Montazeri, Uffe Holmskov, Tohid Kazemi, Pascal Duijf, Morten Gjerstorff, Behzad Baradaran Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A novel multidimensional signature predicts prognosis in hepatocellular carcinoma patients
Using bioinformatics analysis methods including Cox ’s proportional hazards regression analysis, the random survival forest algorithm, Kaplan–Meier and receiver operating characteristic curve analysis, we mined the gene expression profiles of 469 hepatocellular carcinoma (HCC) patients from The Cancer Genome Atlas (n = 379) and Gene Expression Omnibus (GSE14520;n = 90) public database and found a prognostic protein‐coding gene‐microRNA signature that predicts survival in patients with HCC patients. The signature has a bright clinical significance to be a potential prognostic biom...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Song Wang, Jian ‐Hua Zhang, Huan Wang, Lu Yang, Shuai Hong, Bo Yu, Jin‐Cheng Guo, Jing Liu, Yan‐Bing Zhu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Long noncoding RNA NEAT1 promotes the growth of human retinoblastoma cells via regulation of miR ‐204/CXCR4 axis
In conclusion, NEAT1 promotes the development of RB via miR‐204/CXCR4 axis, which provides a new target for the treatment of RB disease. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Wei Zhong, Jingpu Yang, Miyang Li, Lin Li, Aipeng Li Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Aflatoxin G1 induced TNF ‐α‐dependent lung inflammation to enhance DNA damage in alveolar epithelial cells
In this study, we found increased DNA damage and cytochrome P450 (CYP2A13) expression in AFG1‐induced inflamed lung tissues. Furthermore, we treated the mice with a soluble tumor necrosis factor (TNF)‐α receptor and AFG1 and found that TNF ‐α neutralization inhibited the AFG1‐induced chronic lung inflammation in vivo, and then reversed the CYP2A13 expression and DNA damage in AT‐II cells. The results suggest that AFG1 induces TNF ‐α‐dependent lung inflammation to regulate 2A13 expression and enhance DNA damage in AT‐II cells. Then, we treated the primary mice AT‐II cells and human AT‐I...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Peilu Shao, Ningfei Guo, Can Wang, Mei Zhao, Li Yi, Chunping Liu, Lifei Kang, Lei Cao, Ping Lv, Lingxiao Xing, Xianghong Zhang, Haitao Shen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Association between miRNAs expression and signaling pathways of oxidative stress in diabetic retinopathy
AbstractDiabetic retinopathy (DR) is a major cause of vision reduction in diabetic patients. Hyperglycemia is a known instigator for the development of DR, even though the role of oxidative stress pathways in the pathogenesis of DR is established. The studies indicate that microRNAs (miRNAs) are significant to the etiology of DR; changes in miRNAs expression levels may be associated with onset and progression of DR. In addition, miRNAs have emerged as a useful disease marker due to their availability and stability in detecting the severity of DR. The relationship between miRNAs expression levels and oxidative stress pathwa...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Mahbobeh Satari, Esmat Aghadavod, Moein Mobini, Zatollah Asemi Tags: REVIEW ARTICLE Source Type: research

MicroRNA ‐145 induces the senescence of activated hepatic stellate cells through the activation of p53 pathway by ZEB2
In this study, we report that microRNA ‐145 (miR‐145) and p53 were downregulated in vivo and in vitro, concomitant with the enhanced expression of zinc finger E‐box binding homeobox 2 (ZEB2). In addition, overexpression of miR‐145 and p53 led to upregulation of the number of senescence‐associated β‐galactosidase‐positive H SCs and the expression of senescence markers p16 and p21, along with the reduced abundance of HSC activation markers α‐smooth muscle actin and type I collagen in activated HSCs. Furthermore, silencing of ZEB2 promoted senescence of activated HSCs. Moreover, we also demonstrated...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Junfa Yang, Yuchen Lu, Peipei Yang, Qingfeng Chen, Yang Wang, Qi Ding, Tao Xu, Xiaofeng Li, Changyao Li, Cheng Huang, Xiaoming Meng, Jun Li, Lei Zhang, Xiao Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The effects of Nutlin ‐3 on morphology, cellular proliferation, and apoptosis in rat primary mesenchymal stem cells
AbstractIntroductionNutlin ‐3 is a powerful antagonist of murine double minute 2/p53 interaction demonstrating promising therapeutic anticancer activity, which has not been clinically approved yet. Mesenchymal stem cells (MSCs) are an important part of the bone marrow niche and support regeneration and proliferation of hema topoietic stem cells after exposure to myelotoxic anticancer agents; however, the effect of Nutlin‐3 compounds on MSCs themselves remains to be elucidated.Materials and MethodsRat ‐derived bone marrow‐derived MSCs (BMSCs) were cultured and treated with different concentrations (5, 10, 25, 50, an...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Babak Bajelan, Majid Zaki ‐Dizaji, Shahram Darabi, Farzad Rajaei Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Human breast cancer decellularized scaffolds promote epithelial ‐to‐mesenchymal transitions and stemness of breast cancer cells in vitro
In our study, human breast cancer biopsies were decellularized by the detergent technique with sodium lauryl ether sulfate (SLES) solution for the first time. Scaffolds seeded with MCF ‐7 cells demonstrated the process of cell recellularization in vitro, with increased cell migration, proliferation, and epithelial‐to‐mesenchymal transition (EMT) process. When treated with 5‐fluorouracil (5‐Fu), the expressions of stem cell markers, including Oct4, Sox2, and CD49F, were m aximally maintained in the recellularized scaffold with decreased apoptosis rates compared with monolayer cells. AbstractBreast cancer, with uns...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Gang Liu, Biao Wang, Shubin Li, Qin Jin, Yanfeng Dai Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Expression and regulatory networks of Mycobacterium tuberculosis PE/PPE family antigens
Multiple ‐scale integrative analysis revealed the expression and regulatory networks of PE/PPE family antigens underlying the virulence and pathogenesis of Mtb, providing important clues for the discovery of new anti‐TB measures AbstractPE/PPE family antigens are distributed mainly in pathogenic mycobacteria and serve as potential antituberculosis (TB) vaccine components. Some PE/PPE family antigens can regulate the host innate immune response, interfere with macrophage activation and phagolysosome fusion, and serve as major sources of antigenic variation. PE/PPE antigens have been associated with mycobacteria pathogen...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Wu Li, Wanyan Deng, Jianping Xie Tags: MINI ‐REVIEW Source Type: research

Identification of hsa ‐mir‐34a, hsa‐mir‐124, and hsa‐mir‐204 as signatures for cataract
This study aims to explore the key miRNAs underlying the pathogenesis of cataract and find prevention and treatment methods. First, six representative cataract miRNA datasets are selected and the key miRNAs are determined. The target genes of these key miRNAs are predicted using the TargetS can software, and then, the functional enrichment and transcription factor binding analysis of these target genes are completed, respectively. Three signature miRNAs associated with cataract are finally identified. Among them, hsa‐mir‐34a seems to be significantly upregulated, while hsa‐mir‐ 124 and hsa‐mir‐204 are significa...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Ying ‐Li Wei, Hao Sun Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Comparative study of laparoscopic ‐assisted and open total gastrectomy for Siewert Types II and III adenocarcinoma of the esophagogastric junction
AbstractBackgroundThe potential advantages of laparoscopic ‐assisted total gastrectomy (LATG) compared with open total gastrectomy (OTG) for Siewert Types II and III adenocarcinoma of the esophagogastric junction (AEJ) are not very clear. Thus, the aim of this study was to investigate the surgical outcomes and potential advantages of LATG for Siewert Type s II and III AEJ.MethodsThe clinical data of 75 patients (32 for LATG and 43 for OTG) with Siewert II or III AEJ from August 2009 to February 2014 were analyzed retrospectively. Patients were followed up by telephone or out ‐patient examination till August 2015.Result...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Jianchu Wang, Jin ‐Cheng Wang, Bin Song, Xu‐Dong Dai, Xiao‐Yu Zhang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

SP1, MYC, CTNNB1, CREB1, JUN genes as potential therapy targets for neuropathic pain of brain
In conclusion, the pathways of immune resp onse and cytokine‐cytokine receptor interaction were determined as the main metabolic pathways of NP affecting the brain, andSP1, MYC, CTNNB1, CREB1, JUN genes were recognized as the most enriched genes in this process, which may provide evidence for the diagnosis and treatment research of neuropathic pain. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Xue ‐Tao Yan, Yong Xu, Xiao‐Li Cheng, Xiang‐Hu He, Yu Wang, Wen‐Zhong Zheng, Ying Zhao, Hu Chen, Yan‐Lin Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Association between microRNAs expression and signaling pathways of inflammatory markers in diabetic retinopathy
AbstractDiabetic retinopathy is one of the common and serious microvascular complications of diabetes mellitus, as hyperglycemia has metabolic effects on the retina. Hyperglycemia induces increased oxidative stress, which stimulates inflammation pathways and promotes vascular dysfunction of the retina that leads to increased capillary permeability and vascular leakage. One of the main factors involving diabetic retinopathy is the inflammation signaling pathways. In contemporary times, microRNAs (miRNAs) are identified as functional biomarkers for early detection and treatment of numerous diseases specifically diabetic reti...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Rana Shafabakhsh, Esmat Aghadavod, Moein Mobini, Reza Heidari ‐Soureshjani, Zatollah Asemi Tags: MINI ‐REVIEW Source Type: research

Synergistic anticancer action of quercetin and curcumin against triple ‐negative breast cancer cell lines
Women with the breast cancer type 1 susceptibility protein (BRCA1) mutation and loss of BRCA1 expression are reported to have an increased risk of triple ‐negative breast cancer (TNBC). We detected that quercetin and curcumin dose‐dependently enhanced BRCA1 expression. Quercetin and curcumin appeared to induce BRCA1 promoter histone acetylation. Our present study concluded that the combination treatment of quercetin and curcumin acts synergistica lly to induce anticancer activity against TNBC cells by modulating tumor suppressor genes. AbstractWomen with the breast cancer type 1 susceptibility protein (BRCA1) mutation ...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Sai Kundur, Amrita Prayag, Priyanga Selvakumar, Hung Nguyen, Lloyd McKee, Clairissa Cruz, Asha Srinivasan, Sunday Shoyele, Ashakumary Lakshmikuttyamma Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Induced pluripotent stem cell ‐derived extracellular vesicles: A novel approach for cell‐free regenerative medicine
This study reviews the physiological functions of extracellular vesicles and discusses their potential therapeutic benefit in regenerative me dicine. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Behnaz Taheri, Masoud Soleimani, Sedigheh Fekri Aval, Elahe Esmaeili, Zahra Bazi, Nosratollah Zarghami Tags: REVIEW ARTICLE Source Type: research

Glucagon attenuates lipid accumulation in cow hepatocytes through AMPK signaling pathway activation
The ketotic cows displayed hepatic lipid metabolic disorder and high blood concentration of glucagon. The present study indicates that glucagon activates the AMPK signalling pathway to increase lipid oxidation and VLDL assembly and decrease lipid synthesis in cow hepatocytes, thereby reducing liver fat accumulation. AbstractThe ketotic cows displayed hepatic lipid metabolic disorder and high blood concentration of glucagon. Importantly, adenosine monophosphate ‐activated protein kinase (AMPK) signaling pathway plays an important role in the hepatic lipid homeostasis. Therefore, the aim of this study was to investigate th...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yu Li, Hongyan Ding, Jihong Dong, Sajid Ur Rahman, Shibin Feng, Xichun Wang, Jinjie Wu, Zhe Wang, Guowen Liu, Xiaobing Li, Xinwei Li Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Comparative transcriptome analysis reveals PERP upregulated during Salmonella Enteritidis challenge in laying ducks
In this study, the ducks were orally inoculated with SE, and the ovarian follicles and stroma were collected to detect SE infection. The immune responses were triggered and the innate and adaptive immune genes (TLR4, NOD1, Av βD7, and IL‐1β) were upregulated significantly during the SE challenge. Moreover, the ovary tissues (small follicle and stroma) of susceptible and resistant–laying ducks were performed by RNA sequencing. We obtained and identified 23 differentially expressed genes (DEGs) between susceptible an d resistant–laying ducks in both small follicle and stroma tissues (p 
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yu Zhang, Tian ‐tian Gu, Yang Chen, Yu Huang, Jinping Du, Lizhi Lu, Guo‐qiang Zhu, Qi Xu, Guo‐hong Chen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

The role of nitric oxide signaling in renoprotective effects of hydrogen sulfide against chronic kidney disease in rats: Involvement of oxidative stress, autophagy and apoptosis
AbstractThe interplay between H2S and nitric oxide (NO) is thought to contribute to renal functions. The current study was designed to assess the role of NO in mediating the renoprotective effects of hydrogen sulfide in the 5/6 nephrectomy (5/6 Nx) animal model. Forty rats were randomly assigned to 5 experimental groups: (a) Sham; (b) 5/6 Nx; (c) 5/6Nx+sodium hydrosulfide ‐a donor of H2S, (5/6Nx+sodium hydrosulfide [NaHS]); (d) 5/6Nx+NaHS+L‐NAME (a nonspecific nitric oxide synthase [NOS] inhibitor); (e) 5/6Nx+NaHS+aminoguanidine (a selective inhibitor of inducible NOS [iNOS]). Twelve weeks after 5/6 Nx, we assessed the...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Mohammad Khabbaz Shirazi, Asaad Azarnezhad, Mohammad Foad Abazari, Mansour Poorebrahim, Pegah Ghoraeian, Nima Sanadgol, Hanieh Bokharaie, Sahar Heydari, Amin Abbasi, Sahra Kabiri, Maryam Nouri Aleagha, Seyed Ehsan Enderami, Amir Savar Dashtaki Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

EW ‐7197 prevents ulcerative colitis‐associated fibrosis and inflammation
Here, we investigate the potential therapeutic effects of EW ‐7197 in a murine model of ulcerative colitis. Our results suggest that EW‐7197 has potentially useful therapeutic properties against colitis, with clinically translational potential of inhibiting key pathological responses of inflammation and fibrosis in patients with colitis. AbstractEW ‐7197 is a transforming growth factor‐β type I receptor kinase inhibitor with potential anti‐inflammatory and antifibrotic properties. Here, we investigate the potential therapeutic effects of EW‐7197 in a murine model of ulcerative colitis. EW‐7197 attenuated...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Maryam M. Binabaj, Fereshteh Asgharzadeh, Amir Avan, Farzad Rahmani, Atena Soleimani, Mohammad R. Parizadeh, Gordon A. Ferns, Mikhail Ryzhikov, Majid Khazaei, Seyed M. Hassanian Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Poly (3 ‐hydroxybutyrate‐co‐3‐hydroxyvalerate) improved osteogenic differentiation of the human induced pluripotent stem cells while considered as an artificial extracellular matrix
In this study, electrospun PHBV nanofibers were fabricated and characterized and then osteogenic differentiation of the human iPSCs was investigated while cultured on PHBV scaffold.‐MTT, alkaline phosphatase activity, calcium content, gene expression, and western blot evaluations were confirmed osteogenic differentiation of the human iPSCs increased significantly when grown on PHBV nanofibers. AbstractCocell polymers can be the best implants for replacing bone defects in patients. The pluripotent stem cells produced from the patient and the nanofibrous polymeric scaffold that can be completely degraded in the body and it...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Fatemeh Sadat Hosseini, Fatemeh Soleimanifar, Amir Aidun, Seyedeh Elnaz Enderami, Ehsan Saburi, Hadi Zare Marzouni, Mohammad ‐Mehdi Khani, Arash Khojasteh, Abdolreza Ardeshirylajimi Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Antibody –drug conjugates (ADCs) for cancer therapy: Strategies, challenges, and successes
Third ‐generation antibody–drug conjugates are more effective, showing lower levels of unconjugated monoclonal antibodies, more‐stable linkers, improved pharmacokinetic properties, and safety profiles. AbstractTargeted delivery of therapeutic molecules into cancer cells is considered as a promising strategy to tackle cancer. Antibody –drug conjugates (ADCs), in which a monoclonal antibody (mAb) is conjugated to biologically active drugs through chemical linkers, have emerged as a promising class of anticancer treatment agents, being one of the fastest growing fields in cancer therapy. The failure of early A...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Meghdad Abdollahpour ‐Alitappeh, Majid Lotfinia, Tohid Gharibi, Jalal Mardaneh, Behrouz Farhadihosseinabadi, Pegah Larki, Babak Faghfourian, Koushan Sineh Sepehr, Kazem Abbaszadeh‐Goudarzi, Ghasem Abbaszadeh‐Goudarzi, Behrooz Johari, Moha Tags: REVIEW ARTICLE Source Type: research

CtIP promotes G2/M arrest in etoposide ‐treated HCT116 cells in a p53‐independent manner
In this study, we show that knockdown of CtIP, a corepressor of CtBP, promotes cell proliferation and alleviates G2/M phase arrest in etoposide (Eto) ‐treated HCT116 cells. Although the expression of p21 and growth arrest and DNA damage inducible α (GADD45a), which are important targets of p53, was downregulated in CtIP‐deficient HCT116 cells, p53 deletion did not affect G2/M arrest after Eto treatment. In addition, the phosphorylation level s of Ser317 and Ser345 in Chk1 and of Ser216 in CDC25C were lower in CtIP‐deficient HCT116 cells than in control cells after Eto treatment. Our results indicate that CtIP m...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Hongyu Chen, Jin Shan, Dandan Chen, Ruoxi Wang, Wenjing Qi, Hailong Wang, Yueshuang Ke, Wenguang Liu, Xianlu Zeng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Long noncoding RNA LINC00460 promotes carcinogenesis via sponging miR ‐613 in papillary thyroid carcinoma
This study was aimed to ascertain the biological role and molecular mechanism of LINC00460 in PTC progression. Our findings revealed that the level of LINC00460 was significantly upregulated in PTC tissues and cell lines, which was positively correlated with advanced tumor –node–metastasis (TNM) stage and lymph node metastasis. Cellular experiments exhibited that knockdown of LINC00460 decreased proliferative, migratory, and invasive abilities of PTC cells. Mechanism assays noted that knockdown of LINC00460 suppressed cell proliferation, migration, and invasion, a nd inhibited expression of sphingosine kinase 2...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Li Feng, Bin Yang, Xiao ‐Di Tang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Intersectin ‐Cdc42 interaction is required for orderly meiosis in porcine oocytes
Our findings support a model where intersectins, through the interaction with Cdc42, modulates the assembly of meiotic apparatus and actin polymerization, consequently ensuring the orderly meiotic progression during porcine oocyte maturation. AbstractIntersectins (ITSNs) have been shown to act as adaptor proteins that govern multiple cellular events via regulating Cdc42 activity. However, it remains to be determined whether the ITSN ‐Cdc42 pathway is functional in porcine oocytes. To address this question, we used a small molecule, ZCL278, to selectively disrupt the ITSN2‐Cdc42 interaction. In the present study, w...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Xiaoyan Li, Min Gao, Yongfu He, Bo Xiong, Honglin Liu, Ling Gu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

PCNA and JNK1 ‐Stat3 pathways respectively promotes and inhibits diabetes‐associated centrosome amplification by targeting at the ROCK1/14‐3‐3σ complex in human colon cancer HCT116 cells
In conclusion, our results suggest that PCNA and JNK1‐Stat3 pathways respectively promotes and feedback inhibits the centrosome amplificati on by targeting at the ROCK1/14‐3‐3σ complex, and NPM serves as an independent signal for the centrosome amplification. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yu Cheng Lu, Pu Wang, Jie Wang, Ronald Ma, Shao Chin Lee Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

CircRNA ZNF609 functions as a competitive endogenous RNA to regulate FOXP4 expression by sponging miR ‐138‐5p in renal carcinoma
In this study, we investigated whether circ‐ZNF609 may affect cell invasion and proliferation in renal carci noma. Quantitative reverse transcription polymerase chain reaction was performed to detect the expression of circ‐ZNF609 in renal carcinoma cell lines and renal epithelial cells. The direct interaction between microRNA‐138‐5p (miR‐138‐5p) and forkhead box P4 (FOXP4) or circ‐ZNF609 was co nfirmed by luciferase reporter assay and RNA immunoprecipitation assay. We use Cell Counting Kit‐8, 5‐ethynyl‐2′‐deoxyuridine, and Matrigel assays to assess the effect of miR‐138‐5p or circ‐ZNF609 o...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Yunhe Xiong, Jiabin Zhang, Chao Song Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Invasiveness ‐triggered state transition in malignant melanoma cells
AbstractCancer cells are considered to have high morphological heterogeneity in human melanoma tissue. Here, we report that epithelial cancer cells are dominant in different development stages of human melanoma tissues. The cellular and molecular mechanisms that maintain melanoma cells in the epithelial state are further investigated in the A2058 cell line. We find that micropore (8  µm) transwell invasion, but not superficial migration in the scratch assay, can induce remarkable morphological changes between epithelial and mesenchymal melanoma cells within 4 days. The morphological switch is associated with d...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Huan Wang, Yan ‐Guo Zhang, Jing Ma, Jun‐Chang Li, Jian Zhang, Yao‐Qing Yu Tags: RAPID COMMUNICATION Source Type: research

Active vitamin D regulates macrophage M1/M2 phenotypes via the STAT ‐1‐TREM‐1 pathway in diabetic nephropathy
ConclusionVD can inhibit macrophage transition to the M1 phenotype through the STAT ‐1/TREM‐1 pathway. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Xiaoliang Zhang, Yu Zhao, Xiaodong Zhu, Yinfeng Guo, Ying Yang, Yuteng Jiang, Bicheng Liu Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Molecular interaction of NF κB and NICD in monocyte–macrophage differentiation is a target for intervention in atherosclerosis
Inhibition of NF κB regulated notch intracellular domain (NICD), which, in turn, downturned macrophage differentiation. Inhibition of both NFκB–NICD is a potential target for intervention in early stage of atherosclerosis (monocyte to macrophage differentiation), to prevent the furtherance of disease. AbstractThe activation of two transcription factors, NF κB and NICD (notch intracellular domain), plays a crucial role in different stages of atherosclerotic disease progression, from early endothelial activation by modified lipids like oxidized low‐density lipoprotein (oxyLDL) to the imminent ruptur...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Ambika Binesh, Sivasithamparam Niranjali Devaraj, Devaraj Halagowder Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

G protein ‐coupled receptor 119 is involved in RANKL‐induced osteoclast differentiation and fusion
In this study, we i nvestigated the functional role of GPR119 in receptor activator of nuclear factor‐κB ligand (RANKL)‐induced osteoclast formation. We found that the GPR119 expression was markedly increased in preosteoclasts and then downregulated in mature osteoclasts. Activation of GPR119 with AS1269574, a pot ent selective agonist for GPR119, inhibited the generation of multinuclear osteoclasts from bone marrow‐derived macrophages. Confirming this observation, targeted silencing ofGPR119 using short hairpin RNA abrogated the AS1269574 ‐mediated suppressive effect on osteoclast formation. GPR119 activatio...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Hyun ‐Ju Kim, Hye‐Jin Yoon, Ji‐Wan Park, Xiangguo Che, Xian Jin, Je‐Yong Choi Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

MicroRNA: A new player in response to therapy for colorectal cancer
AbstractColorectal cancer (CRC) is one of the important malignancies that result in cancer ‐related deaths worldwide. Multiple lines of evidence have indicated that different responses to therapy in CRC cells led to the failure of the current therapies. Hence, identification of the underlying cellular and molecular pathways involved in the emergence of different responses from CRC cells could contribute to finding and designing new therapeutic platforms to overcome the present limitations. Among the various targets involved in CRC pathogenesis, microRNAs (miRNAs) have key roles in many signaling pathways that are associa...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Mostafa Sarvizadeh, Ziba V. Malekshahi, Ebrahim Razi, Hossein Sharifi, Nousin Moussavi, Mohsen Taghizadeh Tags: REVIEW ARTICLE Source Type: research

Isolation and culture of vascular smooth muscle cells from rat placenta
In this study, we described a simple and efficient method for isolating placental vascular smooth muscle cells (PVSMCs) from rat. The method used the magnetic force between a magnet and ferrous ferric oxide (Fe304) to make the separation and extraction of VSMC. The cells isolated using this protocol were identified as smooth muscle cells (SMCs) by immunoreactivity to the SMC markers and by the “hill and valley” morphology. AbstractWe developed a new separation method for isolating placental vascular smooth muscle cells (PVSMCs) from a rat in this study. Our method used the magnetic force between a magnet and fe...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Chunyu He, Meili Wang, Zi Yan, Suli Zhang, Huirong Liu Tags: FROM THE BENCH Source Type: research

Downregulation of microRNA ‐592 protects mice from hypoplastic heart and congenital heart disease by inhibition of the Notch signaling pathway through upregulating KCTD10
This study demonstrates that downregulation of miR ‐592 prevents CHD and hypoplastic heart by inhibition of the Notch signaling pathway via negatively binding to KCTD10. AbstractEvidence has demonstrated that the microRNA (miR) may play a significant role in the development of congenital heart disease (CHD). Here, we explore the mechanism of microRNA ‐592 (miR‐592) in heart development and CHD with the involvement of KCTD10 and Notch signaling pathway in a CHD mouse model. Cardiac tissues were extracted from CHD and normal mice. Immunohistochemistry staining was performed to detect positive expression rate of KCTD10....
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Xue ‐Feng Pang, Xue Lin, Jian‐Jun Du, Ding‐Yin Zeng Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

α‐2‐Macroglobulin induces the shedding of microvesicles from cutaneous wound myofibroblasts
α‐2‐Macroglobulin positively regulates microvesicle shedding through the activation of low‐density lipoprotein receptor‐related protein‐1 on myofibroblasts. AbstractMicrovesicles (MVs) are recognized as an important class of cell ‐to‐cell messengers. Although the properties of MVs are increasingly documented, the mechanisms regulating MV biogenesis remain debated. Myofibroblasts are a key cellular component of wound healing and have been shown to produce MVs upon stimulation with serum. However, the mediator(s) responsib le for the observed effect of serum on MV release have yet to be identified. To iso...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Alexandra Laberge, Akram Ayoub, Syrine Arif, S ébastien Larochelle, Alain Garnier, Véronique J. Moulin Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Central adiponectin induces trabecular bone mass partly through epigenetic downregulation of cannabinoid receptor CB1
1.Globular  adiponectin (gAPN) enhanced expression levels of various histone deacetylases (HDACs), especially HDAC5. 2.Chromatin immunoprecipitation assays revealed HDAC5 bound to the transcriptional start site 2 (TSS2) region of the CB1 promoter. 3.Our study identified a possible novel central APN‐HDAC5‐ CB1 signaling mechanism that promotes peripheral bone formation through epigenetic regulation of hypothalamic CB1 expression. AbstractCentral adiponectin (APN) in either the globular (gAPN) or full ‐length forms decreases sympathetic tone and increases trabecular bone mass in mice through the hypothalamus. It i...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Hua Jiang, Yuwei Wu, Paloma Valverde, Dana Murray, Jin Tang, Qi Yao, Qianqian Han, Jin Zhang, Lan Zhang, Lei Sui, Yin Tang, Qisheng Tu, Jake Chen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

A novel infram deletion in MSH6 gene in glioma: Conversation on MSH6 mutations in brain tumors
ConclusionPatients should be evaluated for MMR mutation before chemo and radiotherapy, and it is valuable to follow ‐up these mutations during the treatment too. In temozolomide (TMZ)‐resitance cases, it is suggested to use complementary strategies such as using HDACis and a combination of a STAT3 Inhibitor and an mTOR inhibitor, BER inhibition mechanism, and PARP‐1 inhibitor. (Source: Journal of Cellular Physiology)
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Zeinab Deris Zayeri, Maryam Tahmasebi Birgani, Javad Mohammadi Asl, Davood Kashipazha, Mohammadreza Hajjari Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Identification of functional lncRNAs in atrial fibrillation by integrative analysis of the lncRNA ‐mRNA network based on competing endogenous RNAs hypothesis
In this study, we identified eight functional long noncoding RNAs (lncRNAs) for atrial fibrillation based on competing endogenous RNA (ceRNA) hypothesis. These eight lncRNAs showed a good diagnostic power for atrial fibrillation (AF) and could be used as potential biomarkers AbstractA mounting body of evidence has suggested that long noncoding RNAs (lncRNAs) play critical roles in human diseases by acting as competing endogenous RNAs (ceRNAs). However, the functions and ceRNA mechanisms of lncRNAs in atrial fibrillation (AF) remain to date unclear. In this study, we constructed an AF ‐related lncRNA‐mRNA network (AFLMN...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Cheng Qian, Hang Li, Danqi Chang, Baozhu Wei, Yanggan Wang Tags: ORIGINAL RESEARCH ARTICLE Source Type: research

Cardiovascular complications and related risk factors underlying opium consumption
Opium might have deleterious influences on cardiovascular events. As a result, people need to be trained with regard to the adverse effects of opium on cardiovascular events. It may cause hypertension, dyslipidemia, and hormonal abnormalities. AbstractOpium is considered as the second most abused addictive compound in worldwide. It seems that one of the causes for common consumption of opium in many countries is a traditional belief, even among medical personnel, through which opium might have advantageous influences on cardiovascular events and be beneficial in controlling hypertension, dyslipidemia, and diabetes. Accordi...
Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Reyhaneh T. Ebdali, Seyyedeh Samaneh Tabaee, Samira Tabaei Tags: REVIEW ARTICLE Source Type: research