Hydroxypropyl methyl cellulose derivatives stabilize fragment antibody against aggregation in spray dried formulations at elevated temperature and resist pH changes
Eur J Pharm Biopharm. 2022 Jul 30:S0939-6411(22)00152-7. doi: 10.1016/j.ejpb.2022.07.011. Online ahead of print.ABSTRACTThe ability to deliver stable and active dried protein therapeutics from biopharmaceutical drug delivery systems is critical for solid dosage formulation development. Spray dried formulations with carefully selected excipients provide a unique opportunity in amorphous phase stabilization of the therapeutic proteins. Herein, we discuss the role of hydroxypropyl methylcellulose acetate succinate (HPMCAS) derivatives as polymeric excipients for stabilizing a model fragment antibody (Fab2) during high tempera...
Source: European Journal of Pharmaceutics and Biopharmaceutics - August 2, 2022 Category: Drugs & Pharmacology Authors: Purnendu Nayak Karthikan Rajagopal Debby Chang Source Type: research
Effect of Buffer Salts on Physical Stability of Lyophilized and Spray-Dried Protein Formulations Containing Bovine Serum Albumin and Trehalose
This study examined the effect of buffer salts on the physical stability of spray-dried and lyophilized formulations of a model protein, bovine serum albumin (BSA). BSA formulations with various buffers were dried by either lyophilization or spray drying. The protein powders were then characterized using solid-state Fourier transform infrared spectroscopy (ssFTIR), powder X-ray diffraction (PXRD), size exclusion chromatography (SEC), solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS), and solid-state nuclear magnetic resonance spectroscopy (ssNMR). Particle characterizations such as Brunauer-Emmett-T...
Source: Pharmaceutical Research - June 28, 2022 Category: Drugs & Pharmacology Source Type: research
Preparation of a boronate affinity-functionalized metal –organic framework material for selective recognition and separation of glycoproteins at physiological pH
New J. Chem., 2022, Advance Article DOI: 10.1039/D2NJ01182D, PaperBaoyue Zhang, Xue Chen, Jianghua He, Bailin Guo, Sheng Bi, Feng Zhang, Miaomiao Tian A boronate affinity functionalized metal –organic framework material was successfully prepared for the efficient and selective extraction of OVA glycoprotein from egg white samples and protein powder. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - New J. Chem. latest articles)
Source: RSC - New J. Chem. latest articles - June 23, 2022 Category: Chemistry Authors: Baoyue Zhang Source Type: research
Molecules, Vol. 27, Pages 1972: In Vitro Bioaccessibility of Bioactive Compounds from Rosehip-Enriched Corn Extrudates
a Chiş
The rosehip (Rosa canina L.) fruit has gained researchers′ attention due to its rich chemical composition in vitamin C, phenols, carotenoids, and high antioxidant activity; meanwhile, polymers such as pea protein are generally recognized as exhibiting a protection role against the extrusion process. Corn snacks extrudates obtained by replacing corn flour with 10% R. canina powder (R) and 10% R. canina with pea protein (RPP) were evaluated for the physicochemical, textural, optical, and nutritional characteristics. A sample manufactured without R. canina powder was used as a control. Hardness, cris...
Source: Molecules - March 18, 2022 Category: Chemistry Authors: Marta Igual Adriana P ăucean Dan Cristian Vodnar Purificaci ón García-Segovia Javier Mart ínez-Monzó Maria Simona Chi ş Tags: Article Source Type: research
The effect of Bacillus coagulans Unique IS-2 supplementation on plasma amino acid levels and muscle strength in resistance trained males consuming whey protein: a double-blind, placebo-controlled study
ConclusionSignificantly increased absorption of BCAA with supplementation of B. coagulans Unique IS-2 along with whey protein and improvement in leg press and vertical jump power was noted indicating the positive effect of the probiotic on muscle power in the lower body.Trial registration numberCTRI/2017/03/008117; Date:16.03.2017. (Source: European Journal of Nutrition)
Source: European Journal of Nutrition - March 6, 2022 Category: Nutrition Source Type: research
Examination of the association of muscle dysmorphia (bigorexia) and social physique anxiety in the male bodybuilders
This study aimed to examine the association between social physique anxiety and muscular dystrophy in bodybuilders.Design and MethodsThis cross-sectional study included 384 male bodybuilders.Findings5.7% of the participants reported muscular dystrophy. Younger age and use of protein powder were associated with higher scores on the muscle dysmorphic disorder Inventory. Higher muscular dystrophy was associated with higher social physique anxiety.Practice ImplicationsYoung male bodybuilders using protein powder are at increased risk to suffer from social physique anxiety and muscular dystrophy. (Source: Perspectives in Psychiatric Care)
Source: Perspectives in Psychiatric Care - December 3, 2021 Category: Psychiatry Authors: Songul Duran,
Y üksel C. Öz Tags: ORIGINAL ARTICLE Source Type: research
Label-free differentiation and quantification of ricin, abrin from their agglutinin biotoxins by surface plasmon resonance
Talanta. 2022 Feb 1;238(Pt 1):122860. doi: 10.1016/j.talanta.2021.122860. Epub 2021 Sep 9.ABSTRACTHere we describe an affinity molecule-directed surface plasmon resonance (SPR) immunosensor for a label-free, differentiation and quantification of ricin and abrin from their structural highly like agglutinin biotoxins. By an introduction of protein G as the affinity capturing molecule, we fulfilled a complete strategy contains (i) screening monoclonal antibodies to be paired in a sandwiched format, (ii) differentiate quantification from the agglutinin, (iii) ascertain of active from inactive biotoxin, and (iv) structural iden...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Li Luo Jiewei Yang Zhi Li Hua Xu Lei Guo Lili Wang Yuxia Wang Longlong Luo Jing Wang Pingping Zhang Ruifu Yang Weijun Kang Jianwei Xie Source Type: research
Label-free differentiation and quantification of ricin, abrin from their agglutinin biotoxins by surface plasmon resonance
Talanta. 2022 Feb 1;238(Pt 1):122860. doi: 10.1016/j.talanta.2021.122860. Epub 2021 Sep 9.ABSTRACTHere we describe an affinity molecule-directed surface plasmon resonance (SPR) immunosensor for a label-free, differentiation and quantification of ricin and abrin from their structural highly like agglutinin biotoxins. By an introduction of protein G as the affinity capturing molecule, we fulfilled a complete strategy contains (i) screening monoclonal antibodies to be paired in a sandwiched format, (ii) differentiate quantification from the agglutinin, (iii) ascertain of active from inactive biotoxin, and (iv) structural iden...
Source: Talanta - December 3, 2021 Category: Chemistry Authors: Li Luo Jiewei Yang Zhi Li Hua Xu Lei Guo Lili Wang Yuxia Wang Longlong Luo Jing Wang Pingping Zhang Ruifu Yang Weijun Kang Jianwei Xie Source Type: research
IJERPH, Vol. 18, Pages 11939: Comparison of Whey Versus Almond Protein Powder on Nitrogen Balance in Female College Students; The California Almond Protein Powder Project (CAlmond-P3)
. Sikalidis
Plant-based diets have become increasingly popular in the past decade, with approximately 11% of Americans self-identifying as vegan or vegetarian and many others trying to reduce meat consumption. Due to increasing interest, the plant-based food market has significantly expanded, with several innovative products serving as alternatives to animal-based products. One such example is almond protein powder, a new protein supplement created as an alternative to whey protein. Due to the novelty of almond protein products, little is known regarding how well the protein supplement supports nitrogen metabolism. The...
Source: International Journal of Environmental Research and Public Health - November 13, 2021 Category: Environmental Health Authors: Adeline Maykish Morgan M. Nishisaka Courtney K. Talbott Scott K. Reaves Aleksandra S. Kristo Angelos K. Sikalidis Tags: Article Source Type: research
Nanosecond structural dynamics of intrinsically disordered β-casein micelles by neutron spectroscopy
We report on two relaxation processes on a nano-second and a sub-nano-second time scale for β-casein in solution. Both processes are analyzed by Brownian Oscillator model, by which the spring constant can be defined in the isotropic parabolic potential. The slower process, which is analyzed by neutron spin echo, seems a characteristic feature of the unfolded structure. It requires bulk solvent and is not seen in hydrated protein powders. The faster process, which is analyzed by neutron backscattering, has a smaller amplitude and requires hydration water, which is also observed with folded proteins in the hydrated state. T...
Source: Biophysical Journal - October 31, 2021 Category: Physics Authors: Hiroshi Nakagawa Marie-Sousai Appavou Joachim Wuttke Michaela Zamponi Olaf Holderer Tobias E Schrader Dieter Richter Wolfgang Doster Source Type: research
Nanosecond structural dynamics of intrinsically disordered β-casein micelles by neutron spectroscopy
We report on two relaxation processes on a nano-second and a sub-nano-second time scale for β-casein in solution. Both processes are analyzed by Brownian Oscillator model, by which the spring constant can be defined in the isotropic parabolic potential. The slower process, which is analyzed by neutron spin echo, seems a characteristic feature of the unfolded structure. It requires bulk solvent and is not seen in hydrated protein powders. The faster process, which is analyzed by neutron backscattering, has a smaller amplitude and requires hydration water, which is also observed with folded proteins in the hydrated state. T...
Source: Biophysical Journal - October 31, 2021 Category: Physics Authors: Hiroshi Nakagawa Marie-Sousai Appavou Joachim Wuttke Michaela Zamponi Olaf Holderer Tobias E Schrader Dieter Richter Wolfgang Doster Source Type: research
Nanosecond structural dynamics of intrinsically disordered β-casein micelles by neutron spectroscopy
We report on two relaxation processes on a nano-second and a sub-nano-second time scale for β-casein in solution. Both processes are analyzed by Brownian Oscillator model, by which the spring constant can be defined in the isotropic parabolic potential. The slower process, which is analyzed by neutron spin echo, seems a characteristic feature of the unfolded structure. It requires bulk solvent and is not seen in hydrated protein powders. The faster process, which is analyzed by neutron backscattering, has a smaller amplitude and requires hydration water, which is also observed with folded proteins in the hydrated state. T...
Source: Biophysical Journal - October 31, 2021 Category: Physics Authors: Hiroshi Nakagawa Marie-Sousai Appavou Joachim Wuttke Michaela Zamponi Olaf Holderer Tobias E Schrader Dieter Richter Wolfgang Doster Source Type: research
Nanosecond structural dynamics of intrinsically disordered β-casein micelles by neutron spectroscopy
We report on two relaxation processes on a nano-second and a sub-nano-second time scale for β-casein in solution. Both processes are analyzed by Brownian Oscillator model, by which the spring constant can be defined in the isotropic parabolic potential. The slower process, which is analyzed by neutron spin echo, seems a characteristic feature of the unfolded structure. It requires bulk solvent and is not seen in hydrated protein powders. The faster process, which is analyzed by neutron backscattering, has a smaller amplitude and requires hydration water, which is also observed with folded proteins in the hydrated state. T...
Source: Biophysical Journal - October 31, 2021 Category: Physics Authors: Hiroshi Nakagawa Marie-Sousai Appavou Joachim Wuttke Michaela Zamponi Olaf Holderer Tobias E Schrader Dieter Richter Wolfgang Doster Source Type: research
Widespread Supplement Intake and Use of Poor Quality Information in Elite Adolescent Swiss Athletes
In conclusion, this study among elite adolescent Swiss athletes indicates a widespread and large-scale use of dietary supplements, which was associated with a low level of information quality.PMID:34552032 | DOI:10.1123/ijsnem.2021-0043 (Source: International Journal of Sport Nutrition and Exercise Metabolism)
Source: International Journal of Sport Nutrition and Exercise Metabolism - September 23, 2021 Category: Sports Medicine Authors: Samuel Mettler Georgette Lehner Gareth Morgan Source Type: research
