[ASAP] Metabolic Alterations in Postharvest Pear Fruit As Influenced by 1-Methylcyclopropene and Controlled Atmosphere Storage
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.8b04912 (Source: Journal of Agricultural and Food Chemistry)
Source: Journal of Agricultural and Food Chemistry - December 3, 2018 Category: Food Science Authors: Edward J. Flaherty, Geoffrey B. Lum, Jennifer R. DeEll, Sanjeena Subedi, Barry J. Shelp, Gale G. Bozzo Source Type: research
Endophytic bacterial communities of Jingbai Pear trees in north China analyzed with Illumina sequencing of 16S rDNA.
In this study, the structure of endophytic bacterial communities associated with different tissues and soil of Jingbai Pear trees was analyzed with Illumina Miseq sequencing of bacterial 16S rDNA. This is the first report on bacterial microbiome associated with Jingbai pear. Our results demonstrated that different tissues harbored a unique bacterial assemblage. Interestingly, Cyanobacteria was the most abundant phylum, followed by Proteobacteria and Actinobacteria. Samples from three different sites (soils) had significant differences in microbial communities structure. Redundancy analysis (RDA) showed that the bacterial c...
Source: Archives of Microbiology - December 1, 2018 Category: Microbiology Authors: Ren F, Dong W, Yan DH Tags: Arch Microbiol Source Type: research
Chromosome level high-density integrated genetic maps improve the Pyrus bretschneideri ‘DangshanSuli’ v1.0 genome
Chromosomal level reference genomes provide a crucial foundation for genomics research such as genome-wide association studies (GWAS) and whole genome selection. The chromosomal-level sequences of both the Eur... (Source: BMC Genomics)
Source: BMC Genomics - November 21, 2018 Category: Genetics & Stem Cells Authors: Huabai Xue, Suke Wang, Jia-Long Yao, Cecilia H. Deng, Long Wang, Yanli Su, Huirong Zhang, Huangkai Zhou, Minshan Sun, Xiugen Li and Jian Yang Tags: Research article Source Type: research
ATMT transformation efficiencies with native promoters in Botryosphaeria kuwatsukai causing ring rot disease in pear
This study seeks to develop the fungal transformation ofB. kuwatsukai byAgrobacterium tumefaciens-mediated transformation (ATMT), assess the reliability of appropriate vectors and examine the infection processes in vitro using a GFP labeled strain ofB. kuwatsukai. To establish a highly effective transformation system inB. kuwatsukai, binary vectors containing various lengths of H3 promoters and TEF promoters fused with GFP and hygromycin B resistance gene cassettes were constructed. These cassettes were integrated into the genomic DNA ofB. kuwatsukai with high transformation frequency by the ATMT method. Transformants show...
Source: World Journal of Microbiology and Biotechnology - November 19, 2018 Category: Microbiology Source Type: research
Chemical components and bioactivity evaluation of extracts from pear (Pyrus Ussuriensis Maxim) fruit
AbstractIn order to discover the bioactive metabolites ofPyrus ussuriensis Maxim, the systematic chemical and pharmacological investigations were performed. The ethyl acetate extract of the plant displayed activities against ear edema in mice, with an inhibition rate of 17.1%, at a dose of 400mg/kg in vivo. Meanwhile, the ethyl acetate extract showed the best apophlegmatic (inhibition: 49.9%) and antitussive effects (inhibition: 34%) at 800mg/kg in vivo tests, and inhibition of NO production (IC50=0.06 μg/mL). Subsequently, twelve compounds were isolated from the ethyl acetate extract and identified as friedelin (1), dauc...
Source: Journal of Food Biochemistry - October 24, 2018 Category: Food Science Authors: Fei Peng,
Ying Xie,
Xiaojing Li,
Gang Li,
Yuedong Yang Tags: FULL ARTICLE Source Type: research
Dynamic transcriptome analysis of root nitrate starvation and re-supply provides insights into nitrogen metabolism in pear (Pyrus bretschneideri)
This study provides valuable resources for investigating the genetics of the nitrogen metabolic pathways and improving nitrogen utilization efficiency in pear. (Source: Plant Science)
Source: Plant Science - October 10, 2018 Category: Biology Source Type: research
Extension of life-span using a RNAi model and in vivo antioxidant effect of Opuntia fruit extracts and pure betalains in Caenorhabditis elegans
Publication date: 15 February 2019Source: Food Chemistry, Volume 274Author(s): M. Alejandra Guerrero-Rubio, Samanta Hernández-García, Francisco García-Carmona, Fernando Gandía-HerreroAbstractBetalains are nitrogenous plant pigments known for their high antioxidant capacity. For the first time, this antioxidant nature has been studied in an in vivo system using the model organism Caenorhabditis elegans. The oxidative stress caused in the fluorescent strain TJ375 (hsp-16.2::GFP) was reversed by the presence of both natural and semi-synthetic betalains, with an ED50 value around 25 μM for betacyanins and up to 10 μM...
Source: Food Chemistry - September 19, 2018 Category: Food Science Source Type: research
Modelling the growth of pear postharvest fungal isolates at different temperatures.
Abstract
The effect of temperature on the mycelium growth kinetics of four postharvest fungal isolates (i.e., Penicillium expansum, Alternaria alternata, Botrytis cinerea and Rhizopus stolonifer) was assessed. A cardinal model with inflection (CMI) was used to describe the effect of the temperature on the growth rate (μ) and the lag time (λ) of each isolate. Cardinal temperature values such as Tmin, Tmax and Topt were estimated and isolates were sorted according to their growth rate and lag time duration. Additionally, model validation was performed on a medium prepared from mashed pear pulp and on artif...
Source: Food Microbiology - September 1, 2018 Category: Food Science Authors: Sardella D, Gatt R, Valdramidis VP Tags: Food Microbiol Source Type: research
High-resolution genetic linkage map of European pear (Pyrus communis) and QTL fine-mapping of vegetative budbreak time
Genomic analysis technologies can promote efficient fruit tree breeding. Genotyping by sequencing (GBS) enables generating efficient data for high-quality genetic map construction and QTL analysis in a relativ... (Source: BMC Plant Biology)
Source: BMC Plant Biology - August 31, 2018 Category: Biology Authors: Gilad Gabay, Yardena Dahan, Yacov Izhaki, Adi Faigenboim, Giora Ben-Ari, Yonatan Elkind and Moshe A. Flaishman Tags: Research article Source Type: research
Short-term cactus pear [Opuntia ficus-indica (L.) Mill] fruit supplementation ameliorates the inflammatory profile and is associated with improved antioxidant status among healthy humans.
Conclusions: The presently observed modulation of both inflammatory markers and antioxidant balance suggests cactus pear fruit as a novel and beneficial component to be incorporated into current healthy dietary habits.
PMID: 30150921 [PubMed] (Source: Food and Nutrition Research)
Source: Food and Nutrition Research - August 29, 2018 Category: Nutrition Authors: Attanzio A, Tesoriere L, Vasto S, Pintaudi AM, Livrea MA, Allegra M Tags: Food Nutr Res Source Type: research
[ASAP] Characterization of the Genes Involved in Malic Acid Metabolism from Pear Fruit and Their Expression Profile after Postharvest 1-MCP/Ethrel Treatment
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.8b02598 (Source: Journal of Agricultural and Food Chemistry)
Source: Journal of Agricultural and Food Chemistry - August 13, 2018 Category: Food Science Authors: Libin Wang, Min Ma, Yanru Zhang, Zhangfei Wu, Lin Guo, Weiqi Luo, Li Wang, Zhen Zhang, Shaoling Zhang Source Type: research
Network analysis reveals the co-expression of sugar and aroma genes in the Chinese white pear (Pyrus bretschneideri).
This study has demonstrated the power of such network analysis for the first time in a non-model organism. Our research can lead to new discoveries that go beyond the previous focus on individual genes.
PMID: 30099022 [PubMed - as supplied by publisher] (Source: Gene)
Source: Gene - August 9, 2018 Category: Genetics & Stem Cells Authors: Li M, Dunwell JM, Zhang H, Wei S, Li Y, Wu J, Zhang S Tags: Gene Source Type: research
Genes, Vol. 9, Pages 398: Genome-Wide Characterization of DNA Demethylase Genes and Their Association with Salt Response in Pyrus
In this study, we performed genome-wide and deep analysis of putative demethylases (DMEs) in pear. Seven DME genes were found in the pear genome and were defined as PbDME1–7 based on their domain organization. Results were supported by the gene structural characteristics and phylogenetic analysis. The gene structure of the DME genes were relatively complex and the DME7 proteins didn’t contain the Perm_CXXC domain. The DME genes experienced a whole genome duplication event (WGD) that occurred in the ancestor genome of pear and apple before their divergence based on the Ks values. Expression results showed that high sali...
Source: Genes - August 6, 2018 Category: Genetics & Stem Cells Authors: Chunxiao Liu Hui Li Jing Lin Ying Wang Xiaoyang Xu Zong-Ming (Max) Cheng Yonghong Chang Tags: Article Source Type: research
Chemical components and bioactivity evaluation of extracts from pear (Pyrus Ussuriensis Maxim) fruit
Journal of Food Biochemistry, EarlyView. (Source: Journal of Food Biochemistry)
Source: Journal of Food Biochemistry - July 30, 2018 Category: Food Science Authors: Fei
Peng
,
Ying
Xie
,
Xiaojing
Li
,
Gang
Li
,
Yuedong
Yang Source Type: research
Genome-Wide Analysis of Aluminum-Activated Malate Transporter Family Genes in Six Rosaceae Species, and Expression Analysis and Functional Characterization on Malate Accumulation in Chinese White Pear
In this study, a total of 113 ALMT homologous genes were identified from six Rosaceae species (Pyrus bretschneideri, Malus × domestica, Prunus persica, Fragaria vesca, Prunus mume, and Pyrus communis), and 27 of these sequences came from Chinese white pear, designated PbrALMT. Based on the phylogenetic analysis, we divided these ALMT genes into three main clusters (A, B, and C). Conserved domain analysis indicated that all PbrALMT proteins contained the ALMT domain and the FUSC_2 domain, and fewer proteins included the FUSC domain. The results of subcellular localization experiments showed that parts of PbrALMT protei...
Source: Plant Science - July 11, 2018 Category: Biology Source Type: research
