Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols.
Abstract
Theaflavins (TFs) have attracted much attention due to their various bioactivities in black tea. This paper describes the first trial for enzymatic production of TFs by recombinant polyphenol oxidases (PPOs). PPO genes were cloned from nine species and expressed in E. coli. Crude enzyme assays by LC-MS revealed that eight recombinant PPOs were active for TFs production from tea polyphenols as substrates. Much higher activities were observed for crude enzymes of Md2 from Malus domestica (apple), Pp4 from Pyrus pashia (pear), and Ej2 from Eriobotrya japonica (loquat). When immobilized on mesoporous ...
Source: International Journal of Biological Macromolecules - April 21, 2019 Category: Biochemistry Authors: Zeng J, Du G, Shao X, Feng KN, Zeng Y Tags: Int J Biol Macromol Source Type: research
[ASAP] Functional Characterization of a Cystathionine ß-Synthase Gene in Sulfur Metabolism and Pathogenicity of < italic toggle="yes" > Aspergillus niger < /italic > in Pear Fruit
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.9b00325 (Source: Journal of Agricultural and Food Chemistry)
Source: Journal of Agricultural and Food Chemistry - April 12, 2019 Category: Food Science Authors: Shang-Xuan Guo, Gai-Fang Yao, Hui-Ran Ye, Jun Tang, Zhong-Qin Huang, Feng Yang, Yan-Hong Li, Zhuo Han, Lan-Ying Hu, Hua Zhang, Kang-Di Hu Source Type: research
Enhancement of anti-inflammatory and antioxidant activities of prickly pear fruits by high hydrostatic pressure: A chemical and microstructural approach
Publication date: Available online 9 April 2019Source: Innovative Food Science & Emerging TechnologiesAuthor(s): Andrea Gómez-Maqueo, Tomás García-Cayuela, Jorge Welti-Chanes, M. Pilar CanoAbstractPrickly pears were submitted to high hydrostatic pressure (HHP) (pressure: 100, 350 and 600 MPa; temperature: 17–34 °C; time: come-up time (CUT) and 5 min) to enhance their health potential by increasing the extractability of bioactive compounds. Phenolics, betalains and ascorbic acid were analyzed by high performance liquid chromatography (HPLC) and spectrophotometry in peels and in pulps to determine their contribut...
Source: Innovative Food Science and Emerging Technologies - April 10, 2019 Category: Food Science Source Type: research
Insight on Rosaceae Family with Genome Sequencing and Functional Genomics Perspective.
Abstract
Rosaceae is one of the important families possessing a variety of diversified plant species. It includes many economically valuable crops that provide nutritional and health benefits for the human. Whole genome sequences of valuable crop plants were released in recent years. Understanding of genomics helps to decipher the plant physiology and developmental process. With the information of cultivating species and its wild relative genomes, genome sequence-based molecular markers and mapping loci for economically important traits can be used to accelerate the genome assisted breeding. Identification...
Source: Biomed Res - March 28, 2019 Category: Research Authors: Soundararajan P, Won SY, Kim JS Tags: Biomed Res Int Source Type: research
Genome-wide identification, expression and functional analysis of the phosphofructokinase gene family in Chinese white pear (Pyrus bretschneideri).
In this report, 14 phosphofructokinase genes were identified in pear. The phylogenetic tree indicated that the phosphofructokinase gene family could be grouped into two subfamilies, with 10 genes belonging to the PbPFK subfamily, and 4 genes belonging to the PbPFP subfamily. Conserved motifs and exon numbers of the phosphofructokinase were found in pear and other six species. The evolution analysis indicated that WGD/Segmental and dispersed duplications were the main duplication models for the phosphofructokinase genes expansion in pear and other six species. Analysis of cis-regulatory element sequences of all phosphofruct...
Source: Gene - March 19, 2019 Category: Genetics & Stem Cells Authors: Lü H, Li J, Huang Y, Zhang M, Zhang S, Wu J Tags: Gene Source Type: research
A Pyrus communis gene for p-hydroxystyrene biosynthesis, has a role in defense against the pear psylla Cacopsylla biden
Publication date: May 2019Source: Phytochemistry, Volume 161Author(s): Mosaab Yahyaa, Dor Rachmany, Liora Shaltiel-Harpaz, Bhagwat Nawade, Asaf Sadeh, Muhammad Ibdah, Yoram Gerchman, Doron Holland, Mwafaq IbdahAbstractStyrene analogs are known to be naturally synthesized in the leaves of pears and in other plant species, including several trees in the Styracaceae family. Styrene analogs are potential contributors to the aroma of wine, perfumes, pharmaceuticals, and other fermented foods and beverages. In addition, styrene analogs perform important ecological functions such as insecticidal and antifeedant activities against...
Source: Phytochemistry - March 1, 2019 Category: Chemistry Source Type: research
The MYB transcription factor PbMYB12b positively regulates flavonol biosynthesis in pear fruit
As a class of natural antioxidants in plants, fruit flavonol metabolites are beneficial to human health. However, the regulatory networks for flavonol biosynthesis in most fruits are largely unknown. Previousl... (Source: BMC Plant Biology)
Source: BMC Plant Biology - February 21, 2019 Category: Biology Authors: Rui Zhai, Yingxiao Zhao, Meng Wu, Jie Yang, Xieyu Li, Hanting Liu, Ting Wu, Fangfang Liang, Chengquan Yang, Zhigang Wang, Fengwang Ma and Lingfei Xu Tags: Research article Source Type: research
Genes, Vol. 10, Pages 156: Genome-Wide Identification and Analysis of High-Copy-Number LTR Retrotransposons in Asian Pears
i
Luo
A large proportion of the genome of &lsquo;Suli&rsquo; pear (Pyrus pyrifolia) contains long terminal repeat retrotransposons (LTR-RTs), which suggests that LTR-RTs have played important roles in the evolution of Pyrus. Further analysis of retrotransposons, particularly of high-copy-number LTR-RTs in different species, will provide new insights into the evolutionary history of Pyrus. A total of 4912 putative LTR-RTs classified into 198 subfamilies were identified in the &lsquo;Suli&rsquo; pear genome. Six Asian pear accessions, including cultivars and wild species, were resequenc...
Source: Genes - February 18, 2019 Category: Genetics & Stem Cells Authors: Jiang Wang Shi Luo Tags: Article Source Type: research
Family-1 UDP glycosyltransferases in pear (Pyrus bretschneideri): Molecular identification, phylogenomic characterization and expression profiling during stone cell formation.
Abstract
Stone cells are a characteristic trait of pear fruits, and excessive stone cell formation has a significant negative impact on the texture and flavour of the pulp. Lignin is one of the main components of stone cells. Family-1 uridine diphosphate-glycosyltransferases (UGTs) are responsible for the glycosylation modification of monolignols. However, information remains limited regarding the relationship between UGTs and stone cell formation. To address this problem, we identified 139 UGTs from the pear genome, which were distributed in 15 phylogenetic groups (A-M, O, and P). We also performed a coll...
Source: Molecular Biology Reports - February 7, 2019 Category: Molecular Biology Authors: Cheng X, Muhammad A, Li G, Zhang J, Cheng J, Qiu J, Jiang T, Jin Q, Cai Y, Lin Y Tags: Mol Biol Rep Source Type: research
Trehalose increases the oxidative stress tolerance and biocontrol efficacy of Candida oleophila in the microenvironment of pear wounds
Publication date: Available online 30 January 2019Source: Biological ControlAuthor(s): Xiaobao Nie, Changfeng Zhang, Changxing Jiang, Ruichang Zhang, Fengjun Guo, Xinguang FanAbstractHarvested fruit tissues produce reactive oxygen species (ROS) in response to wounding. ROS production is further elevated when biocontrol yeasts are introduced into the wounds, where they act as a bio-elicitor and this results in an environment in which the biocontrol yeast is subject to oxidative stress. In the present study, the effect of trehalose, a non-reducing disaccharide and a major reserve carbohydrate, on the level of oxidative stres...
Source: Biological Control - January 30, 2019 Category: Biology Source Type: research
PbTTG1 forms a ribonucleoprotein complex with polypyrimidine tract-binding protein PbPTB3 to facilitate the long-distance trafficking of PbWoxT1 mRNA
Publication date: Available online 16 January 2019Source: Plant ScienceAuthor(s): Shengnan Wang, Shengyuan Wang, Wenna Zhang, Qiulei Zhang, Li Hao, Yi Zhang, Chaoran Xu, Yunfei Yu, Baoan Wang, Feng Jiang, Tianzhong LiAbstractThe grafting of horticultural crops enables breeders to induce phenotypic changes in rootstocks and scions. A number of signaling molecules, including RNAs and proteins, were recently shown to underlie these changes; however, little is known about the composition of ribonucleoprotein (RNP) complexes or how these macromolecules are transported. Here, we used a polypyrimidine tract-binding protein, PbPTB...
Source: Plant Science - January 18, 2019 Category: Biology Source Type: research
Molecules, Vol. 24, Pages 159: Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS
Zhang
Large quantities of thinned young pears, a natural source of bioactive compounds, are abandoned as agricultural by-products in many orchards. Hence, ten thinned young pear varieties were systematically investigated in terms of their chemical composition and antioxidant potential. Through ultra-performance liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry (UPLC-Q TRAP-MS/MS), 102 polyphenols and 16 triterpenoids were identified and individually quantified within a short time using multiple reaction monitoring (MRM). Subsequently, the antioxidant capacities of these pear...
Source: Molecules - January 3, 2019 Category: Chemistry Authors: Liqiong Sun Shutian Tao Shaoling Zhang Tags: Article Source Type: research
Transcriptomic and evolutionary analyses of white pear (Pyrus bretschneideri) β-amylase genes reveals their importance for cold and drought stress responses.
In this study, we identified 17 PbBAMs in the pear genome. Of these, 12 PbBAM members were mapped onto 9 chromosomes and 5 PbBAM genes were located on scaffold contigs. Based on gene structure, protein motif analysis, and the topology of the phylogenetic tree of the PbBAM family, we classified member genes into 4 groups. All PbBAM genes were found to contain typical glycosyl hydrolysis 14 domain motifs. Interfamilial comparisons revealed that the phylogenetic relationships of BAM genes in other Rosaceae species were similar those found in pear. We also found that whole-genome duplication (WGD)/segmental duplication events ...
Source: Gene - December 19, 2018 Category: Genetics & Stem Cells Authors: Zhao L, Gong X, Gao J, Dong H, Zhang S, Tao S, Huang X Tags: Gene Source Type: research
Canopy attachment position influences metabolism and peel constituency of European pear fruit
Inconsistent pear fruit ripening resulting from variable harvest maturity within tree canopies can contribute to postharvest losses through senescence and spoilage that would otherwise be effectively managed u... (Source: BMC Plant Biology)
Source: BMC Plant Biology - December 18, 2018 Category: Biology Authors: Sara Serra, Nathanael Sullivan, James P. Mattheis, Stefano Musacchi and David R. Rudell Tags: Research article Source Type: research
High Antimicrobial Efficacy, Antioxidant Activity, and a Novel Approach to Phytochemical Analysis of Bioactive Polyphenols in Extracts from Leaves of Pyrus communis and Pyrus pyrifolia Collected During One Vegetative Season
Microbial Drug Resistance, Ahead of Print. (Source: Microbial Drug Resistance)
Source: Microbial Drug Resistance - December 14, 2018 Category: Microbiology Authors: Zbigniew Sroka Gra żyna Zgórka Beata Żbikowska Alina Sowa Roman Franiczek Karolina Wychowaniec Barbara Krzy żanowska Source Type: research
