Family-1 UDP glycosyltransferases in pear (Pyrus bretschneideri): Molecular identification, phylogenomic characterization and expression profiling during stone cell formation.

Family-1 UDP glycosyltransferases in pear (Pyrus bretschneideri): Molecular identification, phylogenomic characterization and expression profiling during stone cell formation. Mol Biol Rep. 2019 Feb 07;: Authors: Cheng X, Muhammad A, Li G, Zhang J, Cheng J, Qiu J, Jiang T, Jin Q, Cai Y, Lin Y Abstract Stone cells are a characteristic trait of pear fruits, and excessive stone cell formation has a significant negative impact on the texture and flavour of the pulp. Lignin is one of the main components of stone cells. Family-1 uridine diphosphate-glycosyltransferases (UGTs) are responsible for the glycosylation modification of monolignols. However, information remains limited regarding the relationship between UGTs and stone cell formation. To address this problem, we identified 139 UGTs from the pear genome, which were distributed in 15 phylogenetic groups (A-M, O, and P). We also performed a collinearity analysis of UGTs among four Rosaceae plants (pear, peach, mei, and strawberry). Phylogenetic analysis suggested that 13 PbUGTs might be related to the glycosylation of monolignols. Analysis of expression patterns demonstrated that most putative monolignol glycosylation-related PbUGTs not only showed high expression levels in flowers and buds but were also induced by exogenous ABA, SA, and MeJA. In addition, the transcript level of Pbr005014.1 (named PbUGT72AJ2) was consistent with the changing trend of lignin content in pear fruit, and...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research