< em > Pyrus communis < /em > L. (Pear) and < em > Malus domestica < /em > Borkh. (apple) leaves lipoidal extracts as sources for beta-sitosterol rich formulae and their wound healing evaluation
Nat Prod Res. 2022 Apr 4:1-5. doi: 10.1080/14786419.2022.2056181. Online ahead of print.ABSTRACTPyrus communis L. (cv. Le-Conte) (pears) and Malus domestica Borkh. (cv. Anna) (apples) are economic fruit crops cultivated in Egypt. Their leaves were assessed for their beta-sitosterol content and found to have 9.4 mg/g dried leaves wt and 5 mg/g dried leaves, respectively. So we used the lipoidal leaves extracts in the formulation of eight beta-sitosterol-rich emulgels from which the most stable formulae were tested for their antimicrobial activity. Finally, the formulae which exerted antimicrobial activity were biologically ...
Source: Natural Product Research - April 4, 2022 Category: Biochemistry Authors: Walaa E Hammam Amany M Gad Mary K Gad Farid N Kirollos Nemat A Yassin Mona Elmahdy El Tantawi Seham Salah Eldin El Hawary Source Type: research
Genome Sequencing of Rahnella victoriana JZ-GX1 Provides New Insights Into Molecular and Genetic Mechanisms of Plant Growth Promotion
Genomic information for bacteria within the genus Rahnella remains limited. Rahnella sp. JZ-GX1 was previously isolated from the Pinus massoniana rhizosphere in China and shows potential as a plant growth-promoting (PGP) bacterium. In the present work, we combined the GridION Nanopore ONT and Illumina sequencing platforms to obtain the complete genome sequence of strain JZ-GX1, and the application effects of the strain in natural field environment was assessed. The whole genome of Rahnella sp. JZ-GX1 comprised a single circular chromosome (5,472,828 bp, G + C content of 53.53%) with 4,483 protein-coding sequences, 22 rRNAs...
Source: Frontiers in Microbiology - March 30, 2022 Category: Microbiology Source Type: research
Viruses, Vol. 14, Pages 599: Seed Transmission of Three Viruses in Two Pear Rootstock Species Pyrus betulifolia and P. calleryana
This study revealed the widespread infection of apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and apple stem pitting virus (ASPV) in maternal trees of P. betulifolia and P. calleryana by nested multiplex reverse transcription-polymerase chain reaction (nmRT-PCR) assays. Seeds from eight P. betulifolia and two P. calleryana trees had positive rates of 15.9&ndash;73.9%, 0&ndash;21.2%, and 40.4% for ASGV, ASPV, and ACLSV, respectively. At the cotyledon and 6&ndash;8 true leaf stages, seedlings grown from seeds of infected trees gave positive rates of 5.4% and 9.3% for ASGV,...
Source: Viruses - March 14, 2022 Category: Virology Authors: Liu Li Lihong Wen Guoping Wang Yuzhuo LYU Zuokun Yang Xiaoping Yang Qingyu Li Ni Hong Tags: Article Source Type: research
Both chitosan and chitooligosaccharide treatments accelerate wound healing of pear fruit by activating phenylpropanoid metabolism
This study aimed to compare the effects of chitosan (CTS) and chitooligosaccharide (COS) treatments on wound healing of pear fruits and to investigate the related mechanisms during postharvest storage under ambient conditions. The results revealed that CTS and COS treatments reduced the weight loss and disease index of the wounded pears (Pyrus bretschneideri cv. Dongguo), and accelerated suberin polyphenolic and lignin deposition at wounds during 7 d of investigation. Furthermore, CTS and COS elevated the level of the genes expression and activities of key enzymes and increased product contents of phenylpropanoid metabolis...
Source: International Journal of Biological Macromolecules - February 23, 2022 Category: Biochemistry Authors: Lirong Yu Yuanyuan Zong Ye Han Xuemei Zhang Yatong Zhu William Oyom Di Gong Dov Prusky Yang Bi Source Type: research
Both chitosan and chitooligosaccharide treatments accelerate wound healing of pear fruit by activating phenylpropanoid metabolism
This study aimed to compare the effects of chitosan (CTS) and chitooligosaccharide (COS) treatments on wound healing of pear fruits and to investigate the related mechanisms during postharvest storage under ambient conditions. The results revealed that CTS and COS treatments reduced the weight loss and disease index of the wounded pears (Pyrus bretschneideri cv. Dongguo), and accelerated suberin polyphenolic and lignin deposition at wounds during 7 d of investigation. Furthermore, CTS and COS elevated the level of the genes expression and activities of key enzymes and increased product contents of phenylpropanoid metabolis...
Source: International Journal of Biological Macromolecules - February 23, 2022 Category: Biochemistry Authors: Lirong Yu Yuanyuan Zong Ye Han Xuemei Zhang Yatong Zhu William Oyom Di Gong Dov Prusky Yang Bi Source Type: research
Genome-wide analysis of the CCT gene family in Chinese white pear (Pyrus bretschneideri Rehd.) and characterization of PbPRR2 in response to varying light signals
Canopy architecture is critical in determining the light environment and subsequently the photosynthetic productivity of fruit crops. Numerous CCT domain-containing genes are crucial for plant adaptive respons... (Source: BMC Plant Biology)
Source: BMC Plant Biology - February 23, 2022 Category: Biology Authors: Zheng Liu, Jia-Li Liu, Lin An, Tao Wu, Li Yang, Yin-Sheng Cheng, Xian-Shuang Nie and Zhong-Qi Qin Tags: Research Source Type: research
Multi-Omics Analysis Reveals the Dynamic Changes of RNA N6-Methyladenosine in Pear (Pyrus bretschneideri) Defense Responses to Erwinia amylovora Pathogen Infection
In this study, MeRIP-seq and RNA-seq data from healthy and inoculated plants were analyzed to assess the changes in the transcript levels and posttranscriptional modification of pear in response to the fire blight pathogen Erwinia amylovora. Following the analysis of 97,261 m6A peaks, we found that m6A preferred to modify duplicate genes rather than singleton genes and that m6A-methylated genes underwent stronger purifying selection. A total of 2,935 specific m6A sites were detected at the transcriptome level after inoculation, which may increase defense-related transcript abundance to enhance pear resistance. In addition,...
Source: Frontiers in Microbiology - February 10, 2022 Category: Microbiology Source Type: research
An ARF1-binding factor triggering programmed cell death and periderm development in pear russet fruit skin
Hortic Res. 2022 Jan 19:uhab061. doi: 10.1093/hr/uhab061. Online ahead of print.ABSTRACTPlants have a cuticular membrane (CM) and periderm membrane (PM), which act as barriers to terrestrial stresses. The CM covers primary organs with a continuous hydrophobic layer of waxes embedded in cutin, while the PM stacks with suberized cells outermost to the secondary tissues. The formation of native periderm is regulated by a postembryonic meristem phellogen that produces suberized phellem (cork) outwardly. However, the mechanism controlling phellogen differentiation to phellem remains to be clarified. Here, map-based cloning in a...
Source: Cell Research - January 19, 2022 Category: Cytology Authors: Yuezhi Wang Meisong Dai Xinyi Wu Shujun Zhang Zebin Shi Danying Cai Lixiang Miao Source Type: research
Robust dynamic experiments for the precise estimation of respiration and fermentation parameters of fruit and vegetables
by Arno Strouwen, Bart M. Nicola ï, Peter Goos
Dynamic models based on non-linear differential equations are increasingly being used in many biological applications. Highly informative dynamic experiments are valuable for the identification of these dynamic models. The storage of fresh fruit and vegetables is one such application where dynamic experimentation is gaining momentum. In this paper, we construct optimal O2 and CO2 gas input profiles to estimate the respiration and fermentation kinetics of pear fruit. The optimal input profiles, however, depend on the true values of the respiration and fermentation parameters....
Source: PLoS Computational Biology - January 12, 2022 Category: Biology Authors: Arno Strouwen Source Type: research
Genome-Wide Identification and Comparative Analysis of the ASR Gene Family in the Rosaceae and Expression Analysis of PbrASRs During Fruit Development
The members of the Abscisic Acid (ABA) Stress and Ripening gene family (ASR) encode a class of plant-specific proteins with ABA/WDS domains that play important roles in fruit ripening, abiotic stress tolerance and biotic stress resistance in plants. The ASR gene family has been widely investigated in the monocotyledons and dicotyledons. Although the genome sequence is already available for eight fruit species of the Rosaceae, there is far less information about the evolutionary characteristics and the function of the ASR genes in the Rosaceae than in other plant families. Twenty-seven ASR genes were identified from species...
Source: Frontiers in Genetics - December 22, 2021 Category: Genetics & Stem Cells Source Type: research
Genome-wide identification and expression analysis of the bZIP transcription factors, and functional analysis in response to drought and cold stresses in pear (Pyrus breschneideri)
Transcription factors (TFs) are involved in many important biological processes, including cell stretching, histological differentiation, metabolic activity, seed storage, gene regulation, and response to abio... (Source: BMC Plant Biology)
Source: BMC Plant Biology - December 9, 2021 Category: Biology Authors: Ming Ma, Qiming Chen, Huizhen Dong, Shaoling Zhang and Xiaosan Huang Tags: Research Source Type: research
Structure, immunostimulatory activity, and the effect of ameliorating airway inflammation of polysaccharides from Pyrus sinkiangensis Yu
Int J Biol Macromol. 2021 Dec 1:S0141-8130(21)02581-2. doi: 10.1016/j.ijbiomac.2021.11.176. Online ahead of print.ABSTRACTPurified acid polysaccharides PSAP-1 and PSAP-2 with apparent molecular weights of 64.6 and 38.9 kDa, respectively, were isolated from Pyrus sinkiangensis Yu. through combined techniques of ion-exchange and gel permeation chromatography. Both polysaccharides were composed of predominant amounts of GalA and small amounts of Ara, Rha, and Gal. They are deduced to be native pectin-type polysaccharides containing the HG backbone consisting of α-1,4-GalAp and methyl-esterified α-1,4-GalAp residues by IR, G...
Source: International Journal of Biological Macromolecules - December 5, 2021 Category: Biochemistry Authors: Hailiqian Taoerdahong Kai Zhou Fei Yang Cai-Xia Dong Source Type: research
Structure, immunostimulatory activity, and the effect of ameliorating airway inflammation of polysaccharides from Pyrus sinkiangensis Yu
Int J Biol Macromol. 2021 Dec 1:S0141-8130(21)02581-2. doi: 10.1016/j.ijbiomac.2021.11.176. Online ahead of print.ABSTRACTPurified acid polysaccharides PSAP-1 and PSAP-2 with apparent molecular weights of 64.6 and 38.9 kDa, respectively, were isolated from Pyrus sinkiangensis Yu. through combined techniques of ion-exchange and gel permeation chromatography. Both polysaccharides were composed of predominant amounts of GalA and small amounts of Ara, Rha, and Gal. They are deduced to be native pectin-type polysaccharides containing the HG backbone consisting of α-1,4-GalAp and methyl-esterified α-1,4-GalAp residues by IR, G...
Source: International Journal of Biological Macromolecules - December 5, 2021 Category: Biochemistry Authors: Hailiqian Taoerdahong Kai Zhou Fei Yang Cai-Xia Dong Source Type: research
Specific detection of Malus- and Pyrus-infecting viroids by real-time reverse-transcription quantitative PCR assays
J Virol Methods. 2021 Nov 30:114395. doi: 10.1016/j.jviromet.2021.114395. Online ahead of print.ABSTRACTViroids present a number of issues for their detection and diagnosis because of the absence of symptom expression in many hosts and their low titers in infected plants. However, quarantine programs rely on symptom observations and routine diagnostic testing to reduce the risk of spreading viroid-infected materials to situations where they might affect crop health and production. Sensitive, accurate, and specific assays for viroid detection from both asymptomatic and symptomatic hosts are necessary for managing viroids in...
Source: Journal of Virological Methods - December 3, 2021 Category: Virology Authors: E Beaver-Kanuya S A Szostek S J Harper Source Type: research
Specific detection of Malus- and Pyrus-infecting viroids by real-time reverse-transcription quantitative PCR assays
J Virol Methods. 2021 Nov 30:114395. doi: 10.1016/j.jviromet.2021.114395. Online ahead of print.ABSTRACTViroids present a number of issues for their detection and diagnosis because of the absence of symptom expression in many hosts and their low titers in infected plants. However, quarantine programs rely on symptom observations and routine diagnostic testing to reduce the risk of spreading viroid-infected materials to situations where they might affect crop health and production. Sensitive, accurate, and specific assays for viroid detection from both asymptomatic and symptomatic hosts are necessary for managing viroids in...
Source: Journal of Virological Methods - December 3, 2021 Category: Virology Authors: E Beaver-Kanuya S A Szostek S J Harper Source Type: research
