Silymarin in combination with ATRA enhances apoptosis induction in human acute promyelocytic NB4 cells
This study investigated the apoptosis-inducing effect of Silymarin (SM) alone and in combination with ATRA on human acute promyelocytic NB4 cells. Examination using MTT assay indicated that SM treatment leads to growth inhibition in NB4 cells in a dose-dependent manner. The IC50 values of SM and ATRA were calculated 90 μM and 2 μM, respectively. Cell cycle analysis by flow cytometry revealed that a more increase in the sub-G1 phase (a sign of apoptosis) when cells were exposed to SM in combination with ATRA. The incidence of apoptosis was confirmed through Hoechst 33258 staining and Annexin V-FITC analysis. The results s...
Source: Toxicon - April 21, 2023 Category: Toxicology Authors: Leila Parsa Rouhollah Motafakkerazad Sarvin Taleb Soheyli Amin Haratian Morteza Kosari-Nasab Majid Mahdavi Source Type: research
Silymarin in combination with ATRA enhances apoptosis induction in human acute promyelocytic NB4 cells
This study investigated the apoptosis-inducing effect of Silymarin (SM) alone and in combination with ATRA on human acute promyelocytic NB4 cells. Examination using MTT assay indicated that SM treatment leads to growth inhibition in NB4 cells in a dose-dependent manner. The IC50 values of SM and ATRA were calculated 90 μM and 2 μM, respectively. Cell cycle analysis by flow cytometry revealed that a more increase in the sub-G1 phase (a sign of apoptosis) when cells were exposed to SM in combination with ATRA. The incidence of apoptosis was confirmed through Hoechst 33258 staining and Annexin V-FITC analysis. The results s...
Source: Toxicon - April 20, 2023 Category: Toxicology Authors: Leila Parsa Rouhollah Motafakkerazad Sarvin Taleb Soheyli Amin Haratian Morteza Kosari-Nasab Majid Mahdavi Source Type: research
Silymarin in combination with ATRA enhances apoptosis induction in human acute promyelocytic NB4 cells
This study investigated the apoptosis-inducing effect of Silymarin (SM) alone and in combination with ATRA on human acute promyelocytic NB4 cells. Examination using MTT assay indicated that SM treatment leads to growth inhibition in NB4 cells in a dose-dependent manner. The IC50 values of SM and ATRA were calculated 90 μM and 2 μM, respectively. Cell cycle analysis by flow cytometry revealed that a more increase in the sub-G1 phase (a sign of apoptosis) when cells were exposed to SM in combination with ATRA. The incidence of apoptosis was confirmed through Hoechst 33258 staining and Annexin V-FITC analysis. The results s...
Source: Toxicon - April 20, 2023 Category: Toxicology Authors: Leila Parsa Rouhollah Motafakkerazad Sarvin Taleb Soheyli Amin Haratian Morteza Kosari-Nasab Majid Mahdavi Source Type: research
Silymarin in combination with ATRA enhances apoptosis induction in human acute promyelocytic NB4 cells
This study investigated the apoptosis-inducing effect of Silymarin (SM) alone and in combination with ATRA on human acute promyelocytic NB4 cells. Examination using MTT assay indicated that SM treatment leads to growth inhibition in NB4 cells in a dose-dependent manner. The IC50 values of SM and ATRA were calculated 90 μM and 2 μM, respectively. Cell cycle analysis by flow cytometry revealed that a more increase in the sub-G1 phase (a sign of apoptosis) when cells were exposed to SM in combination with ATRA. The incidence of apoptosis was confirmed through Hoechst 33258 staining and Annexin V-FITC analysis. The results s...
Source: Toxicon - April 19, 2023 Category: Toxicology Authors: Leila Parsa Rouhollah Motafakkerazad Sarvin Taleb Soheyli Amin Haratian Morteza Kosari-Nasab Majid Mahdavi Source Type: research
990 An integrative approach to understand microcomedone ecosystem and demonstrate the pharmaco-clinical efficacy of an acne prone skin product containing Silybum Marianum Fruit Extract
This study demonstrates the efficacy of an acne prone skin product containing Silybum Marianum Fruit Extract on the restoration of the pilosebaceous follicle ecosystem. Forty subjects with mild-to moderate acne were randomly assigned in two comparative parallel groups: one receiving the tested product twice a day for 56 days, the other consisting in an acne-control group. (Source: Journal of Investigative Dermatology)
Source: Journal of Investigative Dermatology - April 17, 2023 Category: Dermatology Authors: C. Mias, T. Nocera, C. Genies, M. Maitre, C. Lauze, E. Gravier, J. Le Digabel, A. Brocario, A. Simcic Mori, C. Satge, A. Noustens, G. Josse, A. Ortiz-Brugues, S. Bessou-Touya, P. Reygagne, J. Saurat, H. Duplan Source Type: research
Molecules, Vol. 28, Pages 3275: Asteraceae Seeds as Alternative Ingredients in a Fibre-Rich Diet: Protein Quality and Metabolic Effects in Rats
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We verified whether milk thistle seeds and pot marigold seeds provided valuable components for a fibre-rich diet and how their addition affected body composition, nitrogen balance and lipid metabolism in rats. Growing rats were fed a control diet (5% fibre) or three fibre-rich diets (24% fibre), which contained cellulose as the sole source of fibre (24%; positive control), milk thistle seeds (32%) or pot marigold seeds (39%). All diets were balanced in macronutrients, including total protein content (9%), which was half of the amount recommended for rats to maximise protein absorption and utilisation, and the ratio o...
Source: Molecules - April 6, 2023 Category: Chemistry Authors: Jaros ław Koza Adam Jurgo ński Tags: Article Source Type: research
Thiamethoxam evoked neural oxido-inflammatory stress in male rats through modulation of Nrf2/NF-kB/iNOS signaling and inflammatory cytokines: Neuroprotective effect of Silymarin
This study explored the neuroprotective effects of SM against TMX-triggered cortical injury in male rats. Animals were divided into four groups and treated daily either with SM (150 mg/kg), TMX (78.15 mg/kg), or both at the aforementioned doses for 28 days. Our results revealed marked declines in cortical SOD and CAT activities with elevations in MDA, IL-1b and TNF-α levels in TMX-treated rats. Further, TMX induced down-regulation in the gene expressions of Sod, Cat, Gpx, and Nrf-2, with up-regulation in the gene expressions of IL-1b, IL-6, iNOS, TNF-α and NF-kB. Interestingly, pre-treatment with SM provided a notable ne...
Source: Neurotoxicology - March 23, 2023 Category: Neurology Authors: Ola Habotta Ahmed Ateya Rasha M Saleh Eman S El-Ashry Source Type: research
Thiamethoxam Evoked Neural Oxido-inflammatory Stress in Male Rats Through Modulation of Nrf2/NF-kB/iNOS Signaling and Inflammatory Cytokines: Neuroprotective Effect of Silymarin
This study explored the neuroprotective effects of SM against TMX-triggered cortical injury in male rats. Animals were divided into four groups and treated daily either with SM (150mg/kg), TMX (78.15mg/kg), or both at the aforementioned doses for 28 days. Our results revealed marked declines in cortical SOD and CAT activities with elevations in MDA, IL-1b and TNF-α levels in TMX-treated rats. Further, TMX induced down-regulation in the gene expressions of Sod, Cat, Gpx, and Nrf-2, with up-regulation in the gene expressions of IL-1b, IL-6, iNOS, TNF-α and NF-kB. Interestingly, pre-treatment with SM provided a notable neur...
Source: Neurotoxicology - March 23, 2023 Category: Neurology Authors: Ola Habotta Ahmed Ateya Rasha M Saleh Eman S El-Ashry Source Type: research
Molecules, Vol. 28, Pages 2591: Milk Thistle Oil Extracted by Enzyme-Mediated Assisted Solvent Extraction Compared with n-Hexane and Cold-Pressed Extraction
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Silymarin and milk thistle oil have unique biological benefits; however, applying silymarin to milk thistle oil remains a challenge. In this research, the content of silymarin in milk thistle oil conditions using enzyme-mediated solvent extraction was investigated and optimized by response surface methodology. The optimal extraction conditions using enzyme-mediated solvent extraction were as follows: the enzyme-added content was 3.06 mg/mL, the enzymatic hydrolysis temperature was 55.09 °C, and the enzymatic hydrolysis time was 66.28 min. Oil extracted by the enzyme-mediated assisted solvent was further...
Source: Molecules - March 13, 2023 Category: Chemistry Authors: Longlong Liu Hua Zhang Tags: Article Source Type: research
Efficacy of silymarin in patients with non-alcoholic fatty liver disease — the Siliver trial: a study protocol for a randomized controlled clinical trial
Non-alcoholic fatty liver disease (NAFLD) is one of the most prevalent liver diseases globally. Pharmacological treatments for NAFLD are still limited. Silymarin, a compound extracted from Silybum marianum, is an... (Source: Trials)
Source: Trials - March 10, 2023 Category: General Medicine Authors: Camila Ribeiro de Avelar, Beatriz Vieira Coelho Nunes, Betina da Silva Sassaki, Mariana dos Santos Vasconcelos, Lucivalda Pereira Magalh ães de Oliveira, André Castro Lyra, Allain Amador Bueno and Rosângela Passos de Jesus Tags: Study protocol Source Type: research
An integrated transcriptome and microbial community analysis reveals potential mechanisms for increased immune responses when replacing silybum marianum meal with soybean meal in growing lambs
Silybum marianum meal is a by-product that remains silymarin complex and is perceived as a potential-protein source. The potential and its mechanism of silybum marianum meal as a protein supplement in ruminants were evaluated by testing the growth performance, biochemical parameters, cytokine levels, gut transcriptome and microbial community profiles. Forty-two male Hulunbeier growing lambs (aged about 3-month-old; averaged body weight of 21.55 kg) were randomly divided into the CON (with 10% soybean meal) and SIL groups (with 10% silybum marianum meal). There was no significant difference in growth performance, feed int...
Source: Frontiers in Microbiology - March 2, 2023 Category: Microbiology Source Type: research
Pharmacological Interventions for Primary Psychodermatologic Disorders: An Evidence Mapping and Appraisal of Randomized Controlled Trials
CONCLUSION: Few pharmacotherapies for primary psychodermatologic disorders are assessed through controlled trials in the literature. This review serves as a roadmap for researchers and clinicians to reach informed decisions with current evidence, and to build on it to establish guidelines in the future.PMID:36802832 | DOI:10.1177/12034754231155888 (Source: Hand Surgery)
Source: Hand Surgery - February 21, 2023 Category: Surgery Authors: Tarek Turk Chaocheng Liu Esther Fujiwara Sebastian Straube Reidar Hagtvedt Liz Dennett Adam Abba-Aji Marlene Dytoc Source Type: research
Synthesis, characterization, and assessment of chitosan-nanomatrix enriched with antifungal formulation against biodeterioration of active ingredients of selected herbal raw materials
Int J Biol Macromol. 2023 Feb 13:123684. doi: 10.1016/j.ijbiomac.2023.123684. Online ahead of print.ABSTRACTAflatoxin B1 (AFB1), a potent natural group 1 carcinogen produced by Aspergillus flavus is considered an unavoidable toxic contaminant of herbal raw materials, which often deteriorates their active ingredients making them less effective and hazardous during their formulation in herbal drugs. The present investigation reports the antifungal (0.5 μl/ml) and AFB1 inhibitory (0.4 μl/ml) effects of the developed formulation CIM based on a mixture of essential oils (Carum carvi, and Illicium verum), and methyl anthranila...
Source: International Journal of Biological Macromolecules - February 15, 2023 Category: Biochemistry Authors: Vishal Gupta Prem Pratap Singh Bhanu Prakash Source Type: research
Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma
Phytother Res. 2023 Feb 14. doi: 10.1002/ptr.7716. Online ahead of print.ABSTRACTSilybum marianum seeds contain a family of flavonolignans which can regulate cancer cell proliferation and apoptosis. However, research has rarely focused on the effect of S. marianum essential oil in combination with another anticancer drug. Here, we evaluated the antitumor effect of a combination of 5-fluorouracil (5-FU) and S. marianum essential oils on some pathways in hepatocellular carcinoma (HCC) in vitro and in vivo. Gas chromatography-mass spectrography results indicated there was no significant difference between the components of es...
Source: Cancer Control - February 15, 2023 Category: Cancer & Oncology Authors: Mohammad Jaber MasodKhooy Massoumeh Farasat Mohamadreza Salehi Salmi Hamed Mirzaei Source Type: research
Synthesis, characterization, and assessment of chitosan-nanomatrix enriched with antifungal formulation against biodeterioration of active ingredients of selected herbal raw materials
Int J Biol Macromol. 2023 Feb 13:123684. doi: 10.1016/j.ijbiomac.2023.123684. Online ahead of print.ABSTRACTAflatoxin B1 (AFB1), a potent natural group 1 carcinogen produced by Aspergillus flavus is considered an unavoidable toxic contaminant of herbal raw materials, which often deteriorates their active ingredients making them less effective and hazardous during their formulation in herbal drugs. The present investigation reports the antifungal (0.5 μl/ml) and AFB1 inhibitory (0.4 μl/ml) effects of the developed formulation CIM based on a mixture of essential oils (Carum carvi, and Illicium verum), and methyl anthranila...
Source: International Journal of Biological Macromolecules - February 15, 2023 Category: Biochemistry Authors: Vishal Gupta Prem Pratap Singh Bhanu Prakash Source Type: research
