Phenylpropanoids in Silybum marianum cultures treated with cyclodextrins coated with magnetic nanoparticles
In this study, we applied hydroxypropyl-β-CDs (HPCD) and HPCDs coated with magnetic nanoparticles (HPCD-EPI-MN) in methyl jasmonate (MJ)-treated transgenic Silybum marianum cultures ectopically expressing either a stilbene synthase gene (STS) or a chalcone synthase gene (CHS), and compared their effects on the yields of t-R and naringenin (Ng), respectively. HPCD-EPI-MN at 15 g/L stimulated the accumulation of metabolites in the culture medium of the corresponding transgenic cell lines, with up to 4 mg/L of t-R and 3 mg/L of Ng released after 3 days. Similar amounts were produced in cultures treated with HPCD. Concentrati...
Source: Applied Microbiology and Biotechnology - March 28, 2022 Category: Microbiology Authors: Purificaci ón Corchete Lorena Almagro Jose Antonio Gabald ón Mar ía Angeles Pedreño Javier Palaz ón Source Type: research
Phenylpropanoids in Silybum marianum cultures treated with cyclodextrins coated with magnetic nanoparticles
In this study, we applied hydroxypropyl-β-CDs (HPCD) and HPCDs coated with magnetic nanoparticles (HPCD-EPI-MN) in methyl jasmonate (MJ)-treated transgenic Silybum marianum cultures ectopically expressing either a stilbene synthase gene (STS) or a chalcone synthase gene (CHS), and compared their effects on the yields of t-R and naringenin (Ng), respectively. HPCD-EPI-MN at 15 g/L stimulated the accumulation of metabolites in the culture medium of the corresponding transgenic cell lines, with up to 4 mg/L of t-R and 3 mg/L of Ng released after 3 days. Similar amounts were produced in cultures treated with HPCD. Concentrati...
Source: Applied Microbiology and Biotechnology - March 28, 2022 Category: Microbiology Authors: Purificaci ón Corchete Lorena Almagro Jose Antonio Gabald ón Mar ía Angeles Pedreño Javier Palaz ón Source Type: research
Effect of Milk thistle ( < em > Silybum marianum < /em > ) supplementation on the Serum levels of oxidative stress markers in male half marathon athletes
CONCLUSION: SM supplement offered protection against exercise-induced oxidative stress.PMID:35315713 | DOI:10.1080/1354750X.2022.2056921 (Source: Biomarkers)
Source: Biomarkers - March 22, 2022 Category: Research Authors: Fouad Boukazoula Djamila Ayari Source Type: research
Effect of milk thistle ( < em > Silybum marianum < /em > ) supplementation on the serum levels of oxidative stress markers in male half marathon athletes
CONCLUSION: SM supplement offered protection against exercise-induced oxidative stress.PMID:35315713 | DOI:10.1080/1354750X.2022.2056921 (Source: Biomarkers)
Source: Biomarkers - March 22, 2022 Category: Research Authors: Fouad Boukazoula Djamila Ayari Source Type: research
Silybins inhibit human IAPP amyloid growth and toxicity through stereospecific interactions
Biochim Biophys Acta Proteins Proteom. 2022 Mar 17:140772. doi: 10.1016/j.bbapap.2022.140772. Online ahead of print.ABSTRACTType 2 Diabetes is a major public health threat, and its prevalence is increasing worldwide. The abnormal accumulation of islet amyloid polypeptide (IAPP) in pancreatic β-cells is associated with the onset of the disease. Therefore, the design of small molecules able to inhibit IAPP aggregation represents a promising strategy in the development of new therapies. Here we employ in vitro, biophysical, and computational methods to inspect the ability of Silybin A and Silybin B, two natural diastereoisom...
Source: Biochimica et Biophysica Acta - March 21, 2022 Category: Biochemistry Authors: Sara Garc ía-Viñuales Ioana M Ilie Anna Maria Santoro Valeria Romanucci Armando Zarrelli Giovanni Di Fabio Amedeo Caflisch Danilo Milardi Source Type: research
Silybins inhibit human IAPP amyloid growth and toxicity through stereospecific interactions
Biochim Biophys Acta Proteins Proteom. 2022 Mar 17:140772. doi: 10.1016/j.bbapap.2022.140772. Online ahead of print.ABSTRACTType 2 Diabetes is a major public health threat, and its prevalence is increasing worldwide. The abnormal accumulation of islet amyloid polypeptide (IAPP) in pancreatic β-cells is associated with the onset of the disease. Therefore, the design of small molecules able to inhibit IAPP aggregation represents a promising strategy in the development of new therapies. Here we employ in vitro, biophysical, and computational methods to inspect the ability of Silybin A and Silybin B, two natural diastereoisom...
Source: Biochimica et Biophysica Acta - March 21, 2022 Category: Biochemistry Authors: Sara Garc ía-Viñuales Ioana M Ilie Anna Maria Santoro Valeria Romanucci Armando Zarrelli Giovanni Di Fabio Amedeo Caflisch Danilo Milardi Source Type: research
