Dihydromyricetin alleviates Escherichia coli lipopolysaccharide-induced hepatic injury in chickens by inhibiting the NLRP3 inflammasome
AbstractDihydromyricetin (DHM), a flavonoid in vine tea, has many pharmacological activities, including anti-inflammatory and antibacterial effects. Lipopolysaccharide is the key inducer of inflammation in avian pathogenicEscherichia coli (E. coli) infection; however, the effect of DHM onE. coli lipopolysaccharide-induced hepatic injury remains unknown. The present study aimed to explore the role of the NLRP3 inflammasome in hepatic injury and the possible protective mechanisms of DHM against hepatic injury in chickens. The results showed that when chickens were administered lipopolysaccharide, liver damage was observed, a...
Source: Veterinary Research - January 24, 2022 Category: Veterinary Research Source Type: research
African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production
AbstractThe type I interferon (IFN-I) signaling pathway is an important part of the innate immune response and plays a vital role in controlling and eliminating pathogens. African swine fever virus (ASFV) encodes various proteins to evade the host ’s natural immunity. However, the molecular mechanism by which the ASFV-encoded proteins inhibit interferon production remains poorly understood. In the present study, ASFV MGF360-11L inhibited cGAS, STING, TBK1, IKKε, IRF7 and IRF3-5D mediated activation of the IFN-β and ISRE promoters, accompa nied by decreases in IFN-β, ISG15 and ISG56 mRNA expression. ASFV MGF360-11L int...
Source: Veterinary Research - January 24, 2022 Category: Veterinary Research Source Type: research
Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae
AbstractIn recent years,Klebsiella pneumoniae (KP) has caused disease outbreaks in different animals, resulting in serious economic losses and biosafety concerns. Considering the broad antibiotic resistance of KP, vaccines are the most effective tools against infection. However, there is still no KP vaccine available in the veterinary field. Our results indicate that the highly conserved outer membrane phosphoporin (PhoE) of KP is immunogenic in mice and elicits high titers of antibodies that were shown to be specific for PhoE by immunoblotting. Immunization with PhoE also induced robust cell-mediated immunity and elicited...
Source: Veterinary Research - January 21, 2022 Category: Veterinary Research Source Type: research
Bovine respiratory microbiota of feedlot cattle and its association with disease
AbstractBovine respiratory disease (BRD), as one of the most common and costly diseases in the beef cattle industry, has significant adverse impacts on global food security and the economic stability of the industry. The bovine respiratory microbiome is strongly associated with health and disease and may provide insights for alternative therapy when treating BRD. The niche-specific microbiome communities that colonize the inter-surface of the upper and the lower respiratory tract consist of a dynamic and complex ecological system. The correlation between the disequilibrium in the respiratory ecosystem and BRD has become a ...
Source: Veterinary Research - January 12, 2022 Category: Veterinary Research Source Type: research
Luteolin increases susceptibility to macrolides by inhibiting MsrA efflux pump in Trueperella pyogenes
This study aims to evaluate the inhibitory activity of luteolin, a natural flavonoid, on the MsrA efflux pump and investigate its mechanism. The results of antimicrobial susceptibility testing indicated that the susceptibility ofmsrA-positiveT. pyogenes isolates to six macrolides increased after luteolin treatment, while the susceptibility ofmsrA-negative isolates showed no change after luteolin treatment. It is suspected that luteolin may increase the susceptibility ofT. pyogenes isolates by inhibiting MsrA activity. After 1/2 MIC luteolin treatment for 36 h, the transcription level of themsrA gene and the expression le...
Source: Veterinary Research - January 10, 2022 Category: Veterinary Research Source Type: research
