Long-term use of etomidate disrupts the intestinal homeostasis and nervous system in mice
This study aimed to investigate the neurotoxic and gastrointestinal effects of ETO at doses of 1 mg/kg and 3 mg/kg in mice over 14 consecutive days. The results showed that long-term injection of ETO led to drug resistance in mice, affecting their innate preference for darkness and possibly inducing dependence on ETO. The levels of 5-hydroxytryptamine in the brain, serum, and colon decreased by 37%, 51%, and 42% respectively, while the levels of γ-aminobutyric acid reduced by 38%, 52%, and 41% respectively. H&E staining revealed that ETO reduced goblet cells in the colon and damaged the intestinal barrier. The express...
Source: Toxicology - April 11, 2024 Category: Toxicology Authors: Siming Ding Kan Li Xing Han Wenting Lin Yingjun Qin Renjuan Cao Yuan Ren Source Type: research
Differential susceptibility of BRL cells with/without insulin resistance and the role of endoplasmic reticulum stress signaling pathway in response to acrylamide-exposure toxicity effects in vitro
Toxicology. 2024 Apr 9:153800. doi: 10.1016/j.tox.2024.153800. Online ahead of print.ABSTRACTAcrylamide (ACR) is an endogenous food contaminant, high levels of ACR have been detected in a large number of foods, causing widespread concern. Since different organism states respond differently to the toxic effects of pollutants, this study establishes an insulin-resistant BRL cell model to explore the differential susceptibility of BRL cells with/without insulin resistance in response to acrylamide-exposure (0.0002, 0.02, or 1mM) toxicity effects and its mechanism. The results showed that ACR exposure decreased glucose uptake ...
Source: Toxicology - April 11, 2024 Category: Toxicology Authors: Yuchao Guo Houlin Mao Danni Gong Nuo Zhang Dandan Gu Emmanuel Sunday Okeke Weiwei Feng Yao Chen Guanghua Mao Ting Zhao Liuqing Yang Source Type: research
Long-term use of etomidate disrupts the intestinal homeostasis and nervous system in mice
This study aimed to investigate the neurotoxic and gastrointestinal effects of ETO at doses of 1mg/kg and 3mg/kg in mice over 14 consecutive days. The results showed that long-term injection of ETO led to drug resistance in mice, affecting their innate preference for darkness and possibly inducing dependence on ETO. The levels of 5-hydroxytryptamine in the brain, serum, and colon decreased by 37%, 51%, and 42% respectively, while the levels of γ-aminobutyric acid reduced by 38%, 52%, and 41% respectively. H&E staining revealed that ETO reduced goblet cells in the colon and damaged the intestinal barrier. The expressio...
Source: Toxicology - April 11, 2024 Category: Toxicology Authors: Siming Ding Kan Li Xing Han Wenting Lin Yingjun Qin Renjuan Cao Yuan Ren Source Type: research
Differential susceptibility of BRL cells with/without insulin resistance and the role of endoplasmic reticulum stress signaling pathway in response to acrylamide-exposure toxicity effects in vitro
Toxicology. 2024 Apr 9:153800. doi: 10.1016/j.tox.2024.153800. Online ahead of print.ABSTRACTAcrylamide (ACR) is an endogenous food contaminant, high levels of ACR have been detected in a large number of foods, causing widespread concern. Since different organism states respond differently to the toxic effects of pollutants, this study establishes an insulin-resistant BRL cell model to explore the differential susceptibility of BRL cells with/without insulin resistance in response to acrylamide-exposure (0.0002, 0.02, or 1mM) toxicity effects and its mechanism. The results showed that ACR exposure decreased glucose uptake ...
Source: Toxicology - April 11, 2024 Category: Toxicology Authors: Yuchao Guo Houlin Mao Danni Gong Nuo Zhang Dandan Gu Emmanuel Sunday Okeke Weiwei Feng Yao Chen Guanghua Mao Ting Zhao Liuqing Yang Source Type: research
Long-term Bisphenol S exposure induced gut microbiota dysbiosis, obesity, hepatic lipid accumulation, intestinal lesions and dyslipidemia in mice
This study will effectively address the deficiency in toxicological data pertaining to BPS. The novel BPS data obtained from this research can serve as a valuable reference for professionals in the field.PMID:38588857 | DOI:10.1016/j.tox.2024.153798 (Source: Toxicology)
Source: Toxicology - April 8, 2024 Category: Toxicology Authors: Yulang Chi Lin Zhu Yihui Wang Cai Peng Yi Lin Shouping Ji Jie Wei Source Type: research
Long-term Bisphenol S exposure induced gut microbiota dysbiosis, obesity, hepatic lipid accumulation, intestinal lesions and dyslipidemia in mice
This study will effectively address the deficiency in toxicological data pertaining to BPS. The novel BPS data obtained from this research can serve as a valuable reference for professionals in the field.PMID:38588857 | DOI:10.1016/j.tox.2024.153798 (Source: Toxicology)
Source: Toxicology - April 8, 2024 Category: Toxicology Authors: Yulang Chi Lin Zhu Yihui Wang Cai Peng Yi Lin Shouping Ji Jie Wei Source Type: research
Long-term Bisphenol S exposure induced gut microbiota dysbiosis, obesity, hepatic lipid accumulation, intestinal lesions and dyslipidemia in mice
This study will effectively address the deficiency in toxicological data pertaining to BPS. The novel BPS data obtained from this research can serve as a valuable reference for professionals in the field.PMID:38588857 | DOI:10.1016/j.tox.2024.153798 (Source: Toxicology)
Source: Toxicology - April 8, 2024 Category: Toxicology Authors: Yulang Chi Lin Zhu Yihui Wang Cai Peng Yi Lin Shouping Ji Jie Wei Source Type: research
Lnc-Clic5 as a sponge for miR-212-5p to inhibit cow barn PM < sub > 2.5 < /sub > -induced apoptosis in rat alveolar macrophages
In this study, we investigated the effect of exposure to cow barn PM2.5 on rat alveolar macrophages (NR8383) and found that it induced apoptosis via the miR-212-5p/RASSF1 pathway. We found that lnc-Clic5 expression was downregulated in NR8383 cells exposed to cow barn PM2.5. Lnc-Clic5 plays a competitive endogenous RNA (ceRNA) regulatory role by sponging miR-212-5p to attenuate the regulation of RASSF1. Moreover, lnc-Clic5 overexpression inhibited NR8383 apoptosis by targeting the miR-212-5p/RASSF1 pathway. Co-treatment with miR-212-5p and lnc-Clic5 in the presence of cow barn PM2.5 revealed that lnc-Clic5 reversed NR8383 ...
Source: Toxicology - April 7, 2024 Category: Toxicology Authors: Ke Sun Yize Sun Xiaohui Du Xiqing Zhang Zhenhua Ma Yunhang Gao Xiaojun Liang Source Type: research
Lnc-Clic5 as a sponge for miR-212-5p to inhibit cow barn PM < sub > 2.5 < /sub > -induced apoptosis in rat alveolar macrophages
In this study, we investigated the effect of exposure to cow barn PM2.5 on rat alveolar macrophages (NR8383) and found that it induced apoptosis via the miR-212-5p/RASSF1 pathway. We found that lnc-Clic5 expression was downregulated in NR8383 cells exposed to cow barn PM2.5. Lnc-Clic5 plays a competitive endogenous RNA (ceRNA) regulatory role by sponging miR-212-5p to attenuate the regulation of RASSF1. Moreover, lnc-Clic5 overexpression inhibited NR8383 apoptosis by targeting the miR-212-5p/RASSF1 pathway. Co-treatment with miR-212-5p and lnc-Clic5 in the presence of cow barn PM2.5 revealed that lnc-Clic5 reversed NR8383 ...
Source: Toxicology - April 7, 2024 Category: Toxicology Authors: Ke Sun Yize Sun Xiaohui Du Xiqing Zhang Zhenhua Ma Yunhang Gao Xiaojun Liang Source Type: research
Maternal exposure to beta-Cypermethrin disrupts placental development by dysfunction of trophoblast cells from oxidative stress
The objective of this study was to investigate the effect of β-CYP exposure on placental development and the function of trophoblast, as well as the underlying mechanisms through CD-1 mouse model (1, 10, 20mg/kg.bw) and in vitro HTR-8/SVneo cell model (12.5, 25, 50, 100μM). We found slower weight gain and reduced uterine wet weight in pregnant mice with maternal exposure to β-CYP during pregnancy, as well as adverse pregnancy outcomes such as uterine bleeding and embryo resorption. The abnormal placental development in response to β-CYP was noticed, including imbalanced placental structure and disrupted labyrinthine va...
Source: Toxicology - April 6, 2024 Category: Toxicology Authors: Xiaoli Tang Yanqing Geng Rufei Gao Zhuxiu Chen Xinyi Mu Yan Zhang Xin Yin Yidan Ma Xuemei Chen Fangfang Li Junlin He Source Type: research
Maternal exposure to beta-Cypermethrin disrupts placental development by dysfunction of trophoblast cells from oxidative stress
The objective of this study was to investigate the effect of β-CYP exposure on placental development and the function of trophoblast, as well as the underlying mechanisms through CD-1 mouse model (1, 10, 20mg/kg.bw) and in vitro HTR-8/SVneo cell model (12.5, 25, 50, 100μM). We found slower weight gain and reduced uterine wet weight in pregnant mice with maternal exposure to β-CYP during pregnancy, as well as adverse pregnancy outcomes such as uterine bleeding and embryo resorption. The abnormal placental development in response to β-CYP was noticed, including imbalanced placental structure and disrupted labyrinthine va...
Source: Toxicology - April 6, 2024 Category: Toxicology Authors: Xiaoli Tang Yanqing Geng Rufei Gao Zhuxiu Chen Xinyi Mu Yan Zhang Xin Yin Yidan Ma Xuemei Chen Fangfang Li Junlin He Source Type: research
Exploring a mechanism-based approach for the identification of endocrine disruptors using Adverse Outcome Pathways (AOPs) and New Approach Methodologies (NAMs) : A perfluorooctane sulfonic acid case study
Toxicology. 2024 Apr 3;504:153794. doi: 10.1016/j.tox.2024.153794. Online ahead of print.ABSTRACTEndocrine disruptors (EDs) pose a serious threat to human health and the environment and require a comprehensive evaluation to be identified. The identification of EDs require a substantial amount of data, both in vitro and in vivo, due to the current scientific criteria in the EU. At the same time, the EU strives to reduce animal testing due to concerns regarding animal welfare and sensitivity of animal studies to adequately detect adverse effects relevant for human health. Perfluorooctane sulfonic acid (PFOS) is a persistent ...
Source: Toxicology - April 5, 2024 Category: Toxicology Authors: Linus Wiklund Marek P ípal Jana Weiss Anna Beronius Source Type: research
mTORC1 - TFEB pathway was involved in sodium arsenite induced lysosomal alteration, oxidative stress and genetic damage in BEAS-2B cells
Toxicology. 2024 Apr 2:153795. doi: 10.1016/j.tox.2024.153795. Online ahead of print.ABSTRACTThe mechanistic target of rapamycin (RAPA) complex 1 (mTORC1) - transcription factor EB (TFEB) pathway plays a crucial role in response to nutritional status, energy and environmental stress for maintaining cellular homeostasis. But there is few reports on its role in the toxic effects of arsenic exposure and the related mechanisms. Here, we show that the exposure of bronchial epithelial cells (BEAS-2B) to sodium arsenite promoted the activation of mTORC1 (p-mTORC1) and the inactivation of TFEB (p-TFEB), the number and activity of ...
Source: Toxicology - April 4, 2024 Category: Toxicology Authors: Di Ouyang Yiren Xiong Zuqing Hu Jiayi He Shanshan He Renyi Liu Zhenjie Gao Dalin Hu Source Type: research
Toxicological assessment of photoactivated tetra-cationic porphyrin molecules under white light exposure in a Caenorhabditis elegans model
In this study, we used Caenorhabditis elegans, a small free-living nematode, as a model for assessing toxic effects and predicting toxicity in preclinical research. We evaluated the toxic effects of porphyrins (neutral and tetra-cationic) on nematodes under dark/light conditions. Our findings demonstrate that tetra-methylated porphyrins (3TMeP and 4TMeP) at a concentration of 3.3µg/mL (1.36 and 0.93µM) exhibit high toxicity (as evidenced by reduced survival, development, and locomotion) under dark conditions. Moreover, photoactivated tetra-methylated porphyrins induce higher ROS levels compared to neutral (3TPyP and 4TPy...
Source: Toxicology - April 4, 2024 Category: Toxicology Authors: Gabriela Vit ória de Oliveira Marcell Valandro Soares Larissa Marafiga Cordeiro Aline Franzen da Silva Luiza Venturini Larissa Ilha Fabiane Bicca Obetine Baptista T ássia Limana da Silveira F élix Alexandre Antunes Soares Bernardo Almeida Iglesias Source Type: research
mTORC1 - TFEB pathway was involved in sodium arsenite induced lysosomal alteration, oxidative stress and genetic damage in BEAS-2B cells
Toxicology. 2024 Apr 2:153795. doi: 10.1016/j.tox.2024.153795. Online ahead of print.ABSTRACTThe mechanistic target of rapamycin (RAPA) complex 1 (mTORC1) - transcription factor EB (TFEB) pathway plays a crucial role in response to nutritional status, energy and environmental stress for maintaining cellular homeostasis. But there is few reports on its role in the toxic effects of arsenic exposure and the related mechanisms. Here, we show that the exposure of bronchial epithelial cells (BEAS-2B) to sodium arsenite promoted the activation of mTORC1 (p-mTORC1) and the inactivation of TFEB (p-TFEB), the number and activity of ...
Source: Toxicology - April 4, 2024 Category: Toxicology Authors: Di Ouyang Yiren Xiong Zuqing Hu Jiayi He Shanshan He Renyi Liu Zhenjie Gao Dalin Hu Source Type: research
