Selenomethionine protects the liver from dietary deoxynivalenol exposure via Nrf2/PPAR γ-GPX4-ferroptosis pathway in mice
In conclusion, SeMet protected the liver from damage by enhancing the Nrf2/PPARγ-GPX4-ferroptosis pathway, inhibiting lipid accumulation and hepatic lipotoxicity. The findings of this study indicated that SeMet has a positive impact on liver health by improving antioxidant capacity and relieving lipotoxicity in toxin pollution.PMID:38040082 | DOI:10.1016/j.tox.2023.153689 (Source: Toxicology)
Source: Toxicology - December 1, 2023 Category: Toxicology Authors: Shijie Fan Luxi Lin Pingyang Li Huihui Tian Jialu Shen Longzhu Zhou Qingyu Zhao Junmin Zhang Yuchang Qin Chaohua Tang Source Type: research
An adverse outcome pathway-based approach to assess the neurotoxicity by combined exposure to current-use pesticides
Toxicology. 2023 Nov 29:153687. doi: 10.1016/j.tox.2023.153687. Online ahead of print.ABSTRACTExposure to multiple pesticides in daily life has become an important public health concern. However, the combined effects of pesticide mixtures have not been fully elucidated by the conventional toxicological testing used for individual chemicals. Grouping of chemicals by mode of action using common key events (KEs) in the adverse outcome pathway (AOP) as endpoints could be applied for efficient risk assessment of combined exposure to multiple chemicals. The purpose of this study was to investigate whether exposure to multiple pe...
Source: Toxicology - December 1, 2023 Category: Toxicology Authors: Tetsushi Hirano Yoshinori Ikenaka Kei Nomiyama Masato Honda Nobuo Suzuki Nobuhiko Hoshi Yoshiaki Tabuchi Source Type: research
Further development of CometChip technology to measure DNA damage in vitro and in vivo: comparison with the 2 gels/slide format of the standard and enzyme-modified comet assay
Toxicology. 2023 Nov 29:153690. doi: 10.1016/j.tox.2023.153690. Online ahead of print.ABSTRACTDNA damage plays a pivotal role in carcinogenesis and other diseases. The comet assay has been used for more than three decades to measure DNA damages. The 1-2 gels/slide format is the most used version of the assay. In 2010, a high throughput 96 macrowell format with a spatially encoded array of microwells patterned in agarose was developed, called the CometChip. The commercial version (CometChip®) has been used for the in vitro standard version of the comet assay (following the manufacturer's protocol), although it has not been...
Source: Toxicology - December 1, 2023 Category: Toxicology Authors: Miguel Collia Peter M øller Sabine Langie Ariane Vettorazzi Amaya Azqueta Source Type: research
Selenomethionine protects the liver from dietary deoxynivalenol exposure via Nrf2/PPAR γ-GPX4-ferroptosis pathway in mice
In conclusion, SeMet protected the liver from damage by enhancing the Nrf2/PPARγ-GPX4-ferroptosis pathway, inhibiting lipid accumulation and hepatic lipotoxicity. The findings of this study indicated that SeMet has a positive impact on liver health by improving antioxidant capacity and relieving lipotoxicity in toxin pollution.PMID:38040082 | DOI:10.1016/j.tox.2023.153689 (Source: Toxicology)
Source: Toxicology - December 1, 2023 Category: Toxicology Authors: Shijie Fan Luxi Lin Pingyang Li Huihui Tian Jialu Shen Longzhu Zhou Qingyu Zhao Junmin Zhang Yuchang Qin Chaohua Tang Source Type: research
An adverse outcome pathway-based approach to assess the neurotoxicity by combined exposure to current-use pesticides
Toxicology. 2023 Nov 29:153687. doi: 10.1016/j.tox.2023.153687. Online ahead of print.ABSTRACTExposure to multiple pesticides in daily life has become an important public health concern. However, the combined effects of pesticide mixtures have not been fully elucidated by the conventional toxicological testing used for individual chemicals. Grouping of chemicals by mode of action using common key events (KEs) in the adverse outcome pathway (AOP) as endpoints could be applied for efficient risk assessment of combined exposure to multiple chemicals. The purpose of this study was to investigate whether exposure to multiple pe...
Source: Toxicology - December 1, 2023 Category: Toxicology Authors: Tetsushi Hirano Yoshinori Ikenaka Kei Nomiyama Masato Honda Nobuo Suzuki Nobuhiko Hoshi Yoshiaki Tabuchi Source Type: research
Further development of CometChip technology to measure DNA damage in vitro and in vivo: comparison with the 2 gels/slide format of the standard and enzyme-modified comet assay
Toxicology. 2023 Nov 29:153690. doi: 10.1016/j.tox.2023.153690. Online ahead of print.ABSTRACTDNA damage plays a pivotal role in carcinogenesis and other diseases. The comet assay has been used for more than three decades to measure DNA damages. The 1-2 gels/slide format is the most used version of the assay. In 2010, a high throughput 96 macrowell format with a spatially encoded array of microwells patterned in agarose was developed, called the CometChip. The commercial version (CometChip®) has been used for the in vitro standard version of the comet assay (following the manufacturer's protocol), although it has not been...
Source: Toxicology - December 1, 2023 Category: Toxicology Authors: Miguel Collia Peter M øller Sabine Langie Ariane Vettorazzi Amaya Azqueta Source Type: research
A mechanistic toxicology study to grasp the mechanics of zearalenone estrogenicity: spotlighting aromatase and the effects of its genetic variability
Toxicology. 2023 Nov 28:153686. doi: 10.1016/j.tox.2023.153686. Online ahead of print.ABSTRACTZearalenone is a mycoestrogen produced by Fusarium fungi contaminating cereals and in grain-based products threatening human and animal health due to its endocrine disrupting effects. Germane to the mechanisms of action, zearalenone may activate the estrogen receptors and inhibit the estrogens-producing enzyme aromatase (CYP19A1). Both show single nucleotide variants (SNVs) among humans associated with a diverse susceptibility of being activated or inhibited. These variations might modify the endocrine disrupting action of zearale...
Source: Toxicology - November 30, 2023 Category: Toxicology Authors: Florinda Perugino Lorenzo Pedroni Gianni Galaverna Chiara Dall'Asta Luca Dellafiora Source Type: research
A new insight into fluoride induces cardiotoxicity in chickens: involving the regulation of PERK/IRE1/ATF6 pathway and heat shock proteins
This study investigated the effects of long-term exposure to sodium fluoride (0, 500, 1000, and 2000mg/kg) on the hearts of chickens were investigated. The results showed that an elevated exposure dose of sodium fluoride led to congested cardiac tissue and disrupted myofiber organisation. Sodium fluoride exposure activated the ERS pathways of PERK, IRE1, and ATF6, increasing HSP60 and HSP70 and decreasing HSP90. The NF-κB pathway and the activation of TNF-α and iNOS elicited an inflammatory response. BAX, cytc, and cleaved-caspase3 were increased, triggering apoptosis and leading to cardiac injury. The abnormal expressio...
Source: Toxicology - November 30, 2023 Category: Toxicology Authors: Lulu Hou Haiyan Dong Enyu Zhang Hongmin Lu Yue Zhang Hongjing Zhao Mingwei Xing Source Type: research
A mechanistic toxicology study to grasp the mechanics of zearalenone estrogenicity: spotlighting aromatase and the effects of its genetic variability
Toxicology. 2023 Nov 28:153686. doi: 10.1016/j.tox.2023.153686. Online ahead of print.ABSTRACTZearalenone is a mycoestrogen produced by Fusarium fungi contaminating cereals and in grain-based products threatening human and animal health due to its endocrine disrupting effects. Germane to the mechanisms of action, zearalenone may activate the estrogen receptors and inhibit the estrogens-producing enzyme aromatase (CYP19A1). Both show single nucleotide variants (SNVs) among humans associated with a diverse susceptibility of being activated or inhibited. These variations might modify the endocrine disrupting action of zearale...
Source: Toxicology - November 30, 2023 Category: Toxicology Authors: Florinda Perugino Lorenzo Pedroni Gianni Galaverna Chiara Dall'Asta Luca Dellafiora Source Type: research
A new insight into fluoride induces cardiotoxicity in chickens: involving the regulation of PERK/IRE1/ATF6 pathway and heat shock proteins
This study investigated the effects of long-term exposure to sodium fluoride (0, 500, 1000, and 2000mg/kg) on the hearts of chickens were investigated. The results showed that an elevated exposure dose of sodium fluoride led to congested cardiac tissue and disrupted myofiber organisation. Sodium fluoride exposure activated the ERS pathways of PERK, IRE1, and ATF6, increasing HSP60 and HSP70 and decreasing HSP90. The NF-κB pathway and the activation of TNF-α and iNOS elicited an inflammatory response. BAX, cytc, and cleaved-caspase3 were increased, triggering apoptosis and leading to cardiac injury. The abnormal expressio...
Source: Toxicology - November 30, 2023 Category: Toxicology Authors: Lulu Hou Haiyan Dong Enyu Zhang Hongmin Lu Yue Zhang Hongjing Zhao Mingwei Xing Source Type: research
In vitro toxicity of fine and coarse particulate matter on the skin, ocular and lung microphysiological cell-culture systems
This study demonstrates the usefulness of applying co-culture models to assess environmental pollutant toxicity, in addition to being a potential alternative method to animal testing for risk assessment.PMID:38029955 | DOI:10.1016/j.tox.2023.153685 (Source: Toxicology)
Source: Toxicology - November 29, 2023 Category: Toxicology Authors: Dayan Yelena Guerra Laura Margarita Valencia-Osorio Andr és Felipe Zapata-González M ónica Lucía Álvarez-Láinez Elizabeth Cadavid Torres Erick Alejandro Meneses Ramirez Viviana Torres Osorio Juan Sebasti án Botero Valencia Andr és Pareja López Source Type: research
Animal-free assessment of developmental toxicity: Combining PBPK modeling with the ReproTracker assay
Toxicology. 2023 Nov 27;500:153684. doi: 10.1016/j.tox.2023.153684. Online ahead of print.ABSTRACTin vitro screening platforms to assess teratogenic potential of compounds are emerging rapidly. ReproTracker is a human induced pluripotent stem cells (hiPSCs)-based biomarker assay that is shown to identify the teratogenicity potential of new pharmaceuticals and chemicals reliably. In its current state, the assay is limited to identifying the potential teratogenic effects and does not immediately quantify a clinical dose relevant to the exposure of chemicals or drugs observable in mothers or fetuses. The goal of this study wa...
Source: Toxicology - November 29, 2023 Category: Toxicology Authors: Marjory Moreau Amer Jamalpoor John Carter Hall Jeffrey Fisher Sabine Hartvelt Giel Hendriks Andy Nong Source Type: research
Toxicological assessment of E-cigarette flavored E-liquids aerosols using Calu-3 cells: A 3D lung model approach
Toxicology. 2023 Nov 25;500:153683. doi: 10.1016/j.tox.2023.153683. Online ahead of print.ABSTRACTScientific progress and ethical considerations are increasingly shifting the toxicological focus from in vivo animal models to in vitro studies utilizing physiologically relevant cell cultures. Consequently, we evaluated and validated a three-dimensional (3D) model of the human lung using Calu-3 cells cultured at an air-liquid interface (ALI) for 28 days. Assessment of seven essential genes of differentiation and transepithelial electrical resistance (TEER) measurements, in conjunction with mucin (MUC5AC) staining, validated t...
Source: Toxicology - November 28, 2023 Category: Toxicology Authors: Felix Effah John Adragna David Luglio Alexis Bailey Tim Marczylo Terry Gordon Source Type: research
Toxicological assessment of E-cigarette flavored E-liquids aerosols using Calu-3 cells: A 3D lung model approach
Toxicology. 2023 Nov 25;500:153683. doi: 10.1016/j.tox.2023.153683. Online ahead of print.ABSTRACTScientific progress and ethical considerations are increasingly shifting the toxicological focus from in vivo animal models to in vitro studies utilizing physiologically relevant cell cultures. Consequently, we evaluated and validated a three-dimensional (3D) model of the human lung using Calu-3 cells cultured at an air-liquid interface (ALI) for 28 days. Assessment of seven essential genes of differentiation and transepithelial electrical resistance (TEER) measurements, in conjunction with mucin (MUC5AC) staining, validated t...
Source: Toxicology - November 28, 2023 Category: Toxicology Authors: Felix Effah John Adragna David Luglio Alexis Bailey Tim Marczylo Terry Gordon Source Type: research
The variability in CYP3A4 activity determines the metabolic kinetic characteristics of ketamine
In conclusion, variations in CYP3A4 activity would result in the stratification of ketamine blood exposure.PMID:38006927 | DOI:10.1016/j.tox.2023.153682 (Source: Toxicology)
Source: Toxicology - November 25, 2023 Category: Toxicology Authors: Mengfang Li Qingqing Li Dan Lin Xiang Zheng Lehao Jin Jianping Cai Guoxin Hu Jianchang Qian Source Type: research
