Characterization of Arcobacter strains isolated from human stool samples: results from the prospective German prevalence study Arcopath
ConclusionsOur study provides evidence for the abundance of putative virulence genes inArcobacter isolates and prominent cytotoxic effects ofA. butzleri andA. lanthieriin vitro. The presence ofcdtA,cdtB,cdtC inA. lanthieri points towards CDT secretion as potential mechanism underlying cytotoxicity as opposed toA. butzleri. However, the association of theArcobacter virulence factors detected and human morbidity should be addressed in future studies. (Source: Gut Pathogens)
Source: Gut Pathogens - January 7, 2020 Category: Microbiology Source Type: research
Microbial imbalance in inflammatory bowel disease patients at different taxonomic levels
ConclusionsIBD patients exhibited a less diverse gut microbiome compared to healthy individuals. Bacterial groups which changed in IBD patients were found to be groups which do not co-exist well with common commensal gut bacteria, whereas bacterial groups which did not change in patients with IBD were found to commonly co-exist with commensal gut microbiota. This gives a potential insight into the dynamics of the gut microbiota in patients with IBD. (Source: Gut Pathogens)
Source: Gut Pathogens - January 3, 2020 Category: Microbiology Source Type: research
Comparison of different technologies for the decipherment of the whole genome sequence of Campylobacter jejuni BfR-CA-14430
ConclusionThe comparison of Illumina MiSeq, PacBio and MinION sequencing and analyses with different assembly tools enabled us to reconstruct a complete chromosome as well as a circular plasmid sequence of theC. jejuni strain BfR-CA-14430. Illumina short-read sequencing in combination with either PacBio or MinION can substantially improve the quality of the complete chromosome and epichromosomal elements on the level of mismatches and insertions/deletions, depending on the assembly program used. (Source: Gut Pathogens)
Source: Gut Pathogens - December 15, 2019 Category: Microbiology Source Type: research
The intestinal microbial community dissimilarity in hepatitis B virus-related liver cirrhosis patients with and without at alcohol consumption
ConclusionsOur study demonstrated that elevatedFirmicutes/Bacteroidetes ratio, reducedMegamonas genus level and increasedVeillonella genus level were indicators for HBV-related liver cirrhosis. Alcohol-related pathogenesis was associated with the changed microbial community. (Source: Gut Pathogens)
Source: Gut Pathogens - November 25, 2019 Category: Microbiology Source Type: research
Correction to: Involvement of gut microbiome in human health and disease: brief overview, knowledge gaps and research opportunities
Following publication of the original article in Gut Pathogens [1], it was brought to our attention that Dr. Ross Ka Kit Leung ’s affiliation was incomplete. Dr. Leung is appointed as an Adjunct Professor of Guangzhou Medical University from January 1st 2017 to December 31st 2019. He would like to correct here the affiliation and acknowledge his role at the Guangzhou Medical University when this article was published. (Source: Gut Pathogens)
Source: Gut Pathogens - November 20, 2019 Category: Microbiology Source Type: research
Investigation of molecular mechanisms underlying tetracycline resistance in thermophilic Campylobacter spp. suggests that previous reports of tet (A)-mediated resistance in these bacteria are premature
In conclusion, further work would be required to determine whether the homology betweentet(A) potentially present inCampylobacter and knowntet(A) genes would be sufficient to allow amplification using the primers designed in our study. Finally, the existence oftet(A) in thermophilicCampylobacter spp. remains to be demonstrated. (Source: Gut Pathogens)
Source: Gut Pathogens - November 8, 2019 Category: Microbiology Source Type: research
Complete genome analysis of clinical Shigella strains reveals plasmid pSS1653 with resistance determinants: a triumph of hybrid approach
AbstractShigella is ranked as the second leading cause of diarrheal disease worldwide. Though infection occurs in people of all ages, most of the disease burden constitutes among the children less than 5 years in low and middle income countries. Recent increasing incidence of drug resistant strains make this as a priority pathogen under the antimicrobial resistance surveillance by WHO. Despite this, only limited genomic studies on drug resistantShigella exists. Here we report the first complete genome of clinicalS. flexneri serotype 2a andS. sonnei strains using a hybrid approach of both long-read MinION (Oxford Nanopore...
Source: Gut Pathogens - November 5, 2019 Category: Microbiology Source Type: research
Hypervirulent clade 2, ribotype 019/sequence type 67 Clostridioides difficile strain from Japan
ConclusionOur findings contribute to the further understanding of the characteristics of hypervirulent clade 2 including RT019/ST67 lineages. (Source: Gut Pathogens)
Source: Gut Pathogens - November 3, 2019 Category: Microbiology Source Type: research
Occurrence of Escherichia coli and faecal coliforms in drinking water at source and household point-of-use in Rohingya camps, Bangladesh
ConclusionsFrom our findings, it appears that secondary contamination could be a function of very high population density and could possibly occur during collection, transportation, and storage of water due to lack of knowledge of personal and domestic hygiene. Hence, awareness campaign is necessary, and the contaminated sources should be replaced. Further, the POU water should be treated by a suitable method. (Source: Gut Pathogens)
Source: Gut Pathogens - October 31, 2019 Category: Microbiology Source Type: research
RstA, a two-component response regulator, plays important roles in multiple virulence-associated processes in enterohemorrhagic Escherichia coli O157:H7
ConclusionsIn summary, the RstAB TCS in EHEC O157 plays a major role in the regulation of virulence, acid tolerance, and biofilm formation. We clarified the regulatory function of RstA, providing an insight into mechanisms that may be potential drug targets for treatment of EHEC O157-related infections. (Source: Gut Pathogens)
Source: Gut Pathogens - October 31, 2019 Category: Microbiology Source Type: research
Evaluation of the Vibrant DNA microarray for the high-throughput multiplex detection of enteric pathogens in clinical samples
ConclusionThe Vibrant GPP is a comprehensive, high-throughput, and rapid DNA microarray to provide etiologic diagnosis of GI infections in the laboratory setting. (Source: Gut Pathogens)
Source: Gut Pathogens - October 17, 2019 Category: Microbiology Source Type: research
Metagenomics: aid to combat antimicrobial resistance in diarrhea
AbstractAntimicrobial resistance (AMR) has emerged as an obstacle in the supple administration of antimicrobial agents to critical diarrheal patients. Most diarrheal pathogens have developed resistance against the major classes of antibiotics commonly used for assuaging diarrheal symptoms. Antimicrobial resistance develops when pathogens acquire antimicrobial resistance genes (ARGs) through genetic recombination from commensals and pathogens. These are the constituents of the complex microbiota in all ecological niches. The recombination events may occur in the environment or in the gut. Containment of AMR can be achieved ...
Source: Gut Pathogens - October 13, 2019 Category: Microbiology Source Type: research
Is there any association between gut microbiota and type 1 diabetes? A systematic review
ConclusionOur study showed a significant association between alterations in intestinal microbial composition and T1D; however, in some articles, it is not clear which one happens first. Investigation of altered gut microbiota can help in the early detection of T1D before seropositivity. Targeted microbiome modulation can be a novel potential therapeutic strategy. (Source: Gut Pathogens)
Source: Gut Pathogens - October 13, 2019 Category: Microbiology Source Type: research
First complete genome sequence and comparative analysis of Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) indicates host adaptation traits to sheep
ConclusionsWith this sequence, we provide the first complete and closed genome sequence of a SASd strain. With this study, we provide an important basis for an understanding of the genetic mechanism that underlie pathogenicity and host adaptation of SASd to sheep. (Source: Gut Pathogens)
Source: Gut Pathogens - October 13, 2019 Category: Microbiology Source Type: research
Correction to: The pathogenic intestinal spirochaete Brachyspira pilosicoli forms a diverse recombinant species demonstrating some local clustering of related strains and potential for zoonotic spread
The article published in 2013 [1] described the use of the availableBrachyspira multilocus sequence typing (MLST) scheme [2] to characterize the population structure of the intestinal spirocheteBrachyspira pilosicoli. It used sequences of seven loci that were amplified from 131 strains that had been isolated from different geographical origins and species. (Source: Gut Pathogens)
Source: Gut Pathogens - October 13, 2019 Category: Microbiology Source Type: research
