Comparison of different technologies for the decipherment of the whole genome sequence of Campylobacter jejuni BfR-CA-14430

ConclusionThe comparison of Illumina MiSeq, PacBio and MinION sequencing and analyses with different assembly tools enabled us to reconstruct a complete chromosome as well as a circular plasmid sequence of theC. jejuni strain BfR-CA-14430. Illumina short-read sequencing in combination with either PacBio or MinION can substantially improve the quality of the complete chromosome and epichromosomal elements on the level of mismatches and insertions/deletions, depending on the assembly program used.
Source: Gut Pathogens - Category: Microbiology Source Type: research

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(BfR Federal Institute for Risk Assessment) To make food even safer for humans, experts from scientific institutions, food regulatory authorities and the business community will discuss current developments and strategies at the 'Zoonoses and Food Safety' Symposium at the German Federal Institute for Risk Assessment (BfR) on 4 and 5 November 2019, in Berlin-Marienfelde. Because some micro-organisms in food can cause health problems. Campylobacter in raw milk, salmonella in eggs or listeria in ready-to-eat foods often lead to outbreaks affecting numerous patients.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
To better understand public health implications of waterfowl as reservoirs for zoonotic sources of Campylobacter in recreational waters, we developed a Gallus gallus (chick) model of infection to assess the pathogenicity of environmental isolates of Campylobacter. This method involved exposure of 1-day-old chicks through ingestion of water, the natural route of infection. Viable Campylobacter from laboratory-infected animals were monitored by using a modified non-invasive sampling of fresh chick excreta followed by a passive polycarbonate-filter migration culture assay. The method was used to evaluate the infectivities of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion Starter cultures are an important tool that contributes to ensure the safety of fermented meat products. Indeed, the microorganisms that constitute starter cultures may inhibit or reduce the growth of spoilage and/or pathogenic populations through mechanisms, such as production of certain metabolites or competitive exclusion. Thus, the use of starter cultures may reduce the need for chemical additives, such as nitrites and nitrates. Furthermore, the lower residual levels of nitrates and nitrites detected in fermented meat products inoculated with starter cultures are due to the ability of starters to metabolize...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, association rule mining is an effective tool for identifying patterns of multidrug resistance within antimicrobial susceptibility testing data and evaluating the statistical and biological significance of the patterns. Rule quality measures used to sort and differentiate rules should be tested using resampling procedures to minimize the false discovery rate. Rule mining identified consistent multidrug resistance patterns involving beta-lactams, sulfisoxazole, tetracycline, gentamicin, and streptomycin in E. coli isolated from chicken carcasses and meat between 2004 and 2012. The generally low rule overlap su...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Zoonoses such as Brucella, Mycobacterium bovis, Campylobacter, Salmonella, Escherichia coli, Listeria, etc. are spread by ingestion of contaminated unpasteurised milk products (from infected cattle) and result in infectious outbreaks, hospitalisations and mortality. In Southeast Asian countries, raw goat's milk is sometimes used for dengue infection-associated thrombocytopenia; however, evidence to support this sociocultural practice is lacking. A peak incidence of brucellosis cases may occur after a dengue season in communities where this practice is prevalent. PMID: 30895885 [PubMed - as supplied by publisher]
Source: Tropical Doctor - Category: Tropical Medicine Authors: Tags: Trop Doct Source Type: research
In this study, we compared C. jejuni strains isolated from broiler chickens and dairy cattle by determining their ability to adhere to and invade in vitro human colonic epithelial cells in the T84 cell line with their motility, formation of biofilms, and presence of eight virulence genes. A Wilcoxo n Rank Sum test was performed to establish the relationship between presence of the studied genes and cellular invasion and adhesion, as well as differences between the animal species of origin of the isolate. A Spearman correlation was performed to assess the relationship between invasion and motil ity, along with invasion and ...
Source: Canadian Journal of Microbiology - Category: Microbiology Authors: Source Type: research
Author Affiliations open 1Public Health Institute, Oakland, California, USA 2Department of Epidemiology, University of Michigan, Ann Arbor, Michigan, USA 3Instituto de Microbiologia, Colegio de Ciencias Biologicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador 4Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, Michigan, USA 5Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA 6Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, Minnesota, USA PDF Version (176 KB) Abstract ...
Source: EHP Research - Category: Environmental Health Authors: Tags: Brief Communication Source Type: research
Abstract Campylobacter jejuni clone SA is the major cause of sheep abortion and contributes significantly to foodborne illnesses in the United States. Clone SA is hypervirulent because of its distinct ability to produce systemic infection and its predominant role in clinical sheep abortion. Despite the importance of clone SA, little is known about its distribution and epidemiological features in cattle. Here, we describe a prospective study on C. jejuni clone SA prevalence in 35 feedlots in 5 different states in the U.S. and a retrospective analysis of clone SA in C. jejuni isolates collected by National Animal He...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
We report on a case of laboratory‐confirmed Campylobacter jejuni infection in the Commonwealth of Pennsylvania among members of a household living with a laboratory‐confirmed but non‐speciated Campylobacter‐infected puppy. We describe an outbreak of likely dog‐associated campylobacteriosis, the risk factors, potential routes of exposure and the clinical features in the exposed family members, which began shortly after exposure to the recently purchased dog. We also provide public health recommendations to prevent Campylobacter infections in veterinary care providers, pet owners and those planning to adopt pets in...
Source: Zoonoses and Public Health - Category: Infectious Diseases Authors: Tags: ORIGINAL ARTICLE Source Type: research
Conclusions: Our model-based estimates suggest that reducing pathogen prevalence and concentration in source manure would reduce the risk of AGI from exposure to manure irrigation, and that increasing the distance from irrigated manure (i.e., setbacks) and limiting irrigation to times of low wind speed may also reduce risk. https://doi.org/10.1289/EHP283 Received: 30 March 2016 Revised: 07 March 2017 Accepted: 13 March 2017 Published: 16 August 2017 Address Correspondence to M.A. Borchardt, USDA-ARS, 2615 Yellowstone Dr., Marshfield, WI 54449 USA. Telephone: 715-387-4943. Email: Mark.Borchardt@ars.usda.gov Supplement...
Source: EHP Research - Category: Environmental Health Authors: Tags: Research Source Type: research
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