Diversity of 3 ′ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population
ConclusionsIn this study, 503 CagA sequences were studied and analyzed in depth. In Chinese population, mostH. pylori strains were of the CagA-ABD subtype and its presence was associated with gastroduodenal diseases. Amino acid polymorphisms at residues 893 and 894 flanking the EPIYA motifs had a statistically significant association with gastric cancer. (Source: Gut Pathogens)
Source: Gut Pathogens - April 13, 2021 Category: Microbiology Source Type: research
Potential capacity of interferon- α to eliminate covalently closed circular DNA (cccDNA) in hepatocytes infected with hepatitis B virus
AbstractInterferon-alpha (IFN- α) and nucleot(s)ide analogs (NAs) are first-line drugs for the treatment of chronic hepatitis B virus (HBV) infections. Generally, NAs target the reverse transcription of HBV pregenomic RNA, but they cannot eliminate covalently-closed-circular DNA (cccDNA). Although effective treatment with NAs ca n dramatically decrease HBV proteins and DNA loads, and even promote serological conversion, cccDNA persists in the nucleus of hepatocytes due to the lack of effective anti-cccDNA drugs. Of the medications currently available, only IFN-α can potentially target cccDNA. However, the clinical effect...
Source: Gut Pathogens - April 12, 2021 Category: Microbiology Source Type: research
Microbiome profiling of rotavirus infected children suffering from acute gastroenteritis
ConclusionsThe observations assert significant microbial signatures of RVV, which is dose-dependent, and suggest manipulating these microbes as a novel approach for improving RVV efficacy. Further studies are warranted to investigate the immune status of these patients and mechanistic investigation to enhance RVV seroconversion. (Source: Gut Pathogens)
Source: Gut Pathogens - March 29, 2021 Category: Microbiology Source Type: research
Phylogenetic analysis of HIV-1 archived DNA in blood and gut-associated lymphoid tissue in two patients under antiretroviral therapy
AbstractOne of the approaches to cure human immunodeficiency virus (HIV) is the use of therapeutic vaccination. We have launched the Provir/Latitude 45 study to identify conserved CTL epitopes in archived HIV-1 DNA according to the HLA class I alleles in aviremic patients under antiretroviral therapy (ART). A HIV-1 polypeptidic therapeutic vaccine based on viral sequence data obtained from circulating blood was proposed; here, our aim was to compare the proviral DNA in blood and gut-associated lymphoid tissue (GALT). Peripheral blood mononuclear cells and gut biopsies were obtained from two HIV-1 infected patients under su...
Source: Gut Pathogens - March 23, 2021 Category: Microbiology Source Type: research
Mucosa ‐associated cultivable aerobic gut bacterial microbiota among colorectal cancer patients attending at the referral hospitals of Amhara Regional State, Ethiopia
ConclusionsThe overall microbial composition of normal and malignant tissues was considerably different among the study participants. Further culture independent analysis of mucosal microbiota will provide detail pictures of microbial composition differences and pathogenesis of CRC in Ethiopian settings. (Source: Gut Pathogens)
Source: Gut Pathogens - March 22, 2021 Category: Microbiology Source Type: research
Diarrhea in an infant due to Shigella flexneri 1 carrying multiple cephalosporinase-encoding genes
ConclusionsThis report highlights the emergence of extremely resistantShigella that has acquired multiple resistance genes to cephalosporins rendering these drugs ineffective. (Source: Gut Pathogens)
Source: Gut Pathogens - March 20, 2021 Category: Microbiology Source Type: research
Proton pump inhibitors use and risk of developing spontaneous bacterial peritonitis in cirrhotic patients: A systematic review and meta-analysis
ConclusionThis meta-analysis sheds light on the conflicting results raised by previous studies regarding the association of SBP with PPIs use. Our meta-analysis showed that there is a weak association, although statistically significant, between SBP and PPIs use. However, the magnitude of the possible association diminished when analysis focused on higher quality data that were more robust. Thus, this updated meta-analysis suggests judicious use of PPIs among cirrhotic patients with ascites. (Source: Gut Pathogens)
Source: Gut Pathogens - March 19, 2021 Category: Microbiology Source Type: research
No distinction in the gut microbiota between diarrhea predominant-irritable bowel syndrome and healthy subjects: matched case –control study in Thailand
ConclusionAlteration in the gut microbiota composition was probably not the main pathogenic mechanism in the Thai IBS-D patients assessed in this study. However, modifying microbiomes with potentially protective bacteria seems to be a beneficial therapy.Thai Clinical trial registry: TCTR20191211006, Date of registration: 10 Dec 2019. Retrospectively registered, Clinical trial URL:www.clinicaltrials.in.th (Source: Gut Pathogens)
Source: Gut Pathogens - March 3, 2021 Category: Microbiology Source Type: research
Transcriptional regulation of the virulence genes and the biofilm formation associated operons in Vibrio parahaemolyticus
ConclusionQsvR directly repressedtoxR andcalR transcription as well as directly activatedcpsQ-mfpABC andmfpABC transcription. The data presented here promotes us to gain deeper knowledge of the regulatory network of themfp locus inV. parahaemolyticus. (Source: Gut Pathogens)
Source: Gut Pathogens - March 2, 2021 Category: Microbiology Source Type: research
Interplay between severities of COVID-19 and the gut microbiome: implications of bacterial co-infections?
AbstractCOVID-19 is an acute respiratory distress syndrome and is often accompanied by gastrointestinal symptoms. The SARS-CoV-2 has been traced not only in nasopharyngeal and mid-nasal swabs but also in stool and rectal swabs of COVID-19 patients. The gut microbiota is important for an effective immune response as it ensures that unfavorable immune reactions in lungs and other vital organs are regulated. The human gut-lung microbiota interplay provides a framework for therapies in the treatment and management of several pulmonary diseases and infections. Here, we have collated data from COVID-19 studies, which suggest tha...
Source: Gut Pathogens - February 25, 2021 Category: Microbiology Source Type: research
Ectopic gut colonization: a metagenomic study of the oral and gut microbiome in Crohn ’s disease
ConclusionsThere was evidence of ectopic gut colonization by oral bacteria, especially during the active phase of CD. Previously studied gut microbial differences were detected, in addition to novel associations which could have resulted from geographical/ethnic differences to subjects of European descent. Differences in microbial pathways provide possible targets for microbiome modification. (Source: Gut Pathogens)
Source: Gut Pathogens - February 25, 2021 Category: Microbiology Source Type: research
Are hospitalized patients with inflammatory bowel disease at increased risk of invasive bacterial infections? Results from POLIBD 3-year cohort study
AbstractThe aim of this study was to determine the dominant species of bacteria found in blood cultures collected from patients under treatment in the tertiary inflammatory bowel disease (IBD) center in Poland. The dominant pathogen isolated from blood in patients with IBD wasStaphylococcus epidermidis MRCNS (MRCNS —methicillin-resistant coagulase-negativeStaphylococcus), a strain resistant to all beta-lactam antibiotics (penicillins, penicillins with B-lactamase inhibitor, cephalosporins and carbapenems). The second most commonly isolated pathogen found in the blood samples wasEscherichia coli. Blood cultures were found...
Source: Gut Pathogens - February 22, 2021 Category: Microbiology Source Type: research
Gut microbiota of patients with different subtypes of gastric cancer and gastrointestinal stromal tumors
ConclusionsOur analysis shows association of higherEnterobacteriaceae abundance with all types of gastric tumors. Therefore it could be potentially useful as a marker of gastric malignancies. Lower gut microbiota diversity might be indicative of poorly differentiated, invasive, advanced or aggressive tumors and could possibly be a prognostic marker for gastric tumors. (Source: Gut Pathogens)
Source: Gut Pathogens - February 17, 2021 Category: Microbiology Source Type: research
Characteristics of patients infected with Clostridioides difficile at a Saudi Tertiary Academic Medical Center and assessment of antibiotic duration
ConclusionMost patients in this study had hospital-acquired non-severe CDI and were largely exposed to antibiotics and acid suppression therapy. Therefore, such therapies should be revised for necessity. (Source: Gut Pathogens)
Source: Gut Pathogens - February 17, 2021 Category: Microbiology Source Type: research
Comparative genomics of two Shewanella xiamenensis strains isolated from a pilgrim before and during travels to the Hajj
ConclusionsThe whole genome sequences ofS. xiamenensis strain 111B and 111D, including comparative genomic analysis, highlight here the potential for virulence factors that might be related to the cause of diarrhea in humans and also indicate the possible acquisition of pathogenic bacteria, including antibiotic resistance genes or plasmids during the Hajj. (Source: Gut Pathogens)
Source: Gut Pathogens - February 9, 2021 Category: Microbiology Source Type: research
