Prevalence, antimicrobial resistance, and genotyping of Shiga toxin-producing Escherichia coli in foods of cattle origin, diarrheic cattle, and diarrheic humans in Egypt
AbstractShiga toxin-producingEscherichia coli (STEC) is a pathotype ofE. coli that causes enteric and systemic diseases ranging from diarrhoea to severe hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). The emergence of multidrug-resistant (MDR) STEC from cattle sources has increased public health risk and limited treatment options. The prevalence of STEC was investigated in 200 raw food samples (milk and beef samples) and 200 diarrheic samples (cattle and human samples) in a matched region. The presence ofstx genes (stx1 andstx2), carbapenemase-encoding genes (blaVIM,blaNDM-1, andblaIMP), and extended-spectrum...
Source: Gut Pathogens - February 5, 2021 Category: Microbiology Source Type: research
Detection of SARS-CoV-2 from patient fecal samples by whole genome sequencing
ConclusionThese results highlight the potential viability of SARS-CoV-2 in feces, its ongoing mutational accumulation, and its possible role in fecal –oral transmission. This study also elucidates the advantages of SARS-CoV-2 enrichment NGS, which may be a key methodology to document complete viral eradication.Trial registration ClinicalTrials.gov, NCT04359836, Registered 24 April 2020,https://clinicaltrials.gov/ct2/show/NCT04359836?term=NCT04359836&draw=2&rank=1). (Source: Gut Pathogens)
Source: Gut Pathogens - January 30, 2021 Category: Microbiology Source Type: research
The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air
ConclusionPhylogenetic analysis combined with detection of haemolytic (hblA, hblC, andhblD) and non-haemolytic (nheA, nheB, andnheC) enterotoxin genes in both air-isolated strains point to the diarrheic potential of the air isolates, though not emetic. Characterization of these airborne strains and investigation of their potential disease-causing genes could facilitate identification of environmental sources of contamination leading to foodborne illnesses and nosocomial infections transported by air. (Source: Gut Pathogens)
Source: Gut Pathogens - January 30, 2021 Category: Microbiology Source Type: research
Adherent invasive Escherichia coli (AIEC) strain LF82, but not Candida albicans , plays a profibrogenic role in the intestine
ConclusionsThese results demonstrate that AIEC strain LF82, but notC. albicans, may play a major profibrogenic role in the gut. (Source: Gut Pathogens)
Source: Gut Pathogens - January 28, 2021 Category: Microbiology Source Type: research
Evaluation of CAMPYLOBACTER QUIK CHEK ™ rapid membrane enzyme immunoassay to detect Campylobacter spp. antigen in stool samples
AbstractCampylobacter spp. enteritis is the most frequent bacterial enteritis in both adults and children and is sometimes a source of severe complications. Its diagnosis by culture suffers from a lack of sensitivity and delays the result, preventing an early initiation of optimal antibiotic therapy in some cases. Our aim was to test a new rapid immuno-enzymatic method forCampylobacter spp. diagnosis in comparison to a composite reference standard (CRS). Stool samples from the French National Reference Center for Campylobacter and Helicobacter were tested with the CAMPYLOBACTER QUIK CHEK ™ (Abbott). The CRS used to consi...
Source: Gut Pathogens - January 22, 2021 Category: Microbiology Source Type: research
Bacteroides , butyric acid and t10,c12-CLA changes in colorectal adenomatous polyp patients
ConclusionThe gut microbiota analysis results, assessed in faeces, showed thatBacteroides andCitrobacter were positively correlated with CAPs, which indicated that changes in specific genera might be detrimental to intestinal health. In addition,t10,c12-CLA played an important role in protecting the intestine. (Source: Gut Pathogens)
Source: Gut Pathogens - January 12, 2021 Category: Microbiology Source Type: research
High genetic diversity in Campylobacter concisus isolates from patients with microscopic colitis
AbstractThe emerging intestinal pathogenCampylobacter concisus has been associated with prolonged diarrhoea and classic inflammatory bowel diseases (IBD) and was recently also linked with microscopic colitis (MC). Previous reports have observed a high genetic diversity within isolates from diarrhoeic and IBD patients and from healthy controls (HC), and division of isolates into two major genomospecies (GS1 and GS2). The aim of this study was to describe genetic diversity in 80 recently cultivated MC biopsy and faecal isolates ofC. concisus by multi-locus sequence typing (MLST); and to compare the phylogenetic relatedness t...
Source: Gut Pathogens - January 12, 2021 Category: Microbiology Source Type: research
Eubacterium rectale contributes to colorectal cancer initiation via promoting colitis
ConclusionsOur findings reveal thatE. rectale functions as a ‘driver’ bacterium and contributes to cancer initiation via promoting inflammation. (Source: Gut Pathogens)
Source: Gut Pathogens - January 12, 2021 Category: Microbiology Source Type: research
Comparative genomics of Helicobacter pullorum from different countries
ConclusionIn this study, we obtained the genetic characteristics ofH. pullorum from different sources in the world. The comprehensive genetic characteristics ofH. pullorum were first described.H. pullorum showed highly genetic diversity and two sub-types of T6SSs were first identified inH. pullorum. 2013BJHL was found to be multidrug resistant as it was resistant to at least three different antibiotic classes. (Source: Gut Pathogens)
Source: Gut Pathogens - December 10, 2020 Category: Microbiology Source Type: research
Transcriptome changes and polymyxin resistance of acid-adapted Escherichia coli O157:H7 ATCC 43889
ConclusionThe transcriptomic and phenotypic changes analysed in this study during the adaptation ofE. coli to acid environments can provide useful information for developing intervention technologies and mitigating the risk associated with the emergence and spread of antimicrobial resistance. (Source: Gut Pathogens)
Source: Gut Pathogens - December 1, 2020 Category: Microbiology Source Type: research
Genomic patterns and characterizations of chromosomally-encoded mcr - 1 in Escherichia coli populations
In this study, we systematically investigated the genetic basis of chromosomally-encodedmcr-1 in prevalence and potential mechanisms of lineage, plasmid, insertion sequence, and phage. Our results demonstrated that the emergence of chromosomally-encodedmcr-1 could originate from multiple mechanisms, but mainly derived through the recombination of ISApl1/Tn6330. We reported a specific transmission mechanism, which is a phage-like region without lysogenic components, could associate with the emergence and stabilization of chromosomally-encodedmcr-1. These results highlighted the potential origin and risks of chromosomally-en...
Source: Gut Pathogens - November 28, 2020 Category: Microbiology Source Type: research
Emergence of plasmid-mediated mcr genes from Gram-negative bacteria at the human-animal interface
ConclusionsThe detection of a considerable number ofmcr-1 andmcr-2 genes in animals is worrisome, as they are now being detected in clinical pathogens. The acquisition ofmcr genes by colistin-susceptible bacteria could leave us in a post-antibiotic era. (Source: Gut Pathogens)
Source: Gut Pathogens - November 20, 2020 Category: Microbiology Source Type: research
The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection
ConclusionThe study findings showed that the absence of mCRAMP modulated both host responses and the enteric microbiota enhancing local and systemic infection bySalmonella Typhimurium. (Source: Gut Pathogens)
Source: Gut Pathogens - November 13, 2020 Category: Microbiology Source Type: research
Stool and sputum microbiome during quinolone prophylaxis of spontaneous bacterial peritonitis: an exploratory study
ConclusionThe present exploratory study with small sample size showed that inter-individual differences in diversity of gut microbiota were high at baseline, yet quinolone prophylaxis had only a moderate impact. High relative abundances ofEnterobacteriaceae during follow-up might indicate failure of or non-adherence to quinolone prophylaxis. However, our results may not be clinically significant given the limitations of the study and therefore future studies are needed to further investigate this phenomenon. (Source: Gut Pathogens)
Source: Gut Pathogens - October 30, 2020 Category: Microbiology Source Type: research
Potential role of intestinal microflora in disease progression among patients with different stages of Hepatitis B
ConclusionsFaecal microbiota transplantation of some potential beneficial bacteria can change with the occurrence of disease, and HBV carriers might be the most suitable donors. (Source: Gut Pathogens)
Source: Gut Pathogens - October 27, 2020 Category: Microbiology Source Type: research
