Understanding Horizontal Gene Transfer network in human gut microbiota
ConclusionHence, we proposed the HGT network constructions from the gut microbiota sequencing data. The HGT networks capture the host state and the response of microbiota to the environmental and host changes, and they are essential to understand the human microbiota. (Source: Gut Pathogens)
Source: Gut Pathogens - July 8, 2020 Category: Microbiology Source Type: research
Development of risk factor-based scoring system for detection of hypervirulent Klebsiella pneumoniae bloodstream infections
ConclusionsSimple scoring system was developed for the diagnosis of HVKp infections. The system allows early identification of patients withK. pneumoniae BSIs in whom hypervirulent infections should be evaluated. Prospective evaluation is expected. (Source: Gut Pathogens)
Source: Gut Pathogens - July 8, 2020 Category: Microbiology Source Type: research
Metagenomic analysis of gut microbiome and resistome of diarrheal fecal samples from Kolkata, India, reveals the core and variable microbiota including signatures of microbial dark matter
ConclusionsThis is the first comparative study of the gut microbiome associated with different diarrheal pathogens. It presents the first catalogue of different bacterial taxa representing the core and variable microbiome in acute diarrheal patients. The study helped to define a trend in the gut microbiota signature associated with diarrhea and revealed which ARGs are abundantly present and the metagenome-assembled genomes (MAGs) contributing to AMR. (Source: Gut Pathogens)
Source: Gut Pathogens - July 6, 2020 Category: Microbiology Source Type: research
CagA orchestrates eEF1A1 and PKC δ to induce interleukin-6 expression in Helicobacter pylori -infected gastric epithelial cells
ConclusionsCagA promotes the expression of IL-6 in AGS cells by recruiting PKC δ through eEF1A1 in the cytoplasm to increase the phosphorylation of STAT3S727 in the nucleus. These findings provide new insights into the function of CagA-eEF1A1 interaction in gastric adenocarcinoma. (Source: Gut Pathogens)
Source: Gut Pathogens - July 2, 2020 Category: Microbiology Source Type: research
Stool cultures show a lack of impact in the management of acute gastroenteritis for hospitalized patients in the Bronx, New York
ConclusionStool cultures are commonly ordered when AGE is suspected. In our
cohort, stool culture had a very low yield of detecting an underlying pathogen. Although
patients who had stool cultures obtained were more likely to be febrile and to have a
longer length of hospital stay than were those who did not have stool cultures, for the
vast majority of patients, stool culture played little to no role in patient management.
Further studies are needed to which patients benefit most from undergoing stool
culture. (Source: Gut Pathogens)
Source: Gut Pathogens - June 21, 2020 Category: Microbiology Source Type: research
Development of a highly effective low-cost vaporized hydrogen peroxide-based method for disinfection of personal protective equipment for their selective reuse during pandemics
ConclusionsOur study, therefore, successfully establishes the utility of VHP to effectively disinfect PPE for a possible reuse as per the requirements. VHP treatment did not damage coveralls, cause physical deformity and also did not alter fabric architecture of melt blown layer. We observed that disinfection process was successful consistently and therefore believe that the VHP-based decontamination model will have a universal applicability and utility. This process can be easily and economically scaled up and can be instrumental in easing global PPE shortages in any biosafety facility or in health care settings during pa...
Source: Gut Pathogens - June 18, 2020 Category: Microbiology Source Type: research
Toxigenic and non-toxigenic patterns I, II and III and biofilm-forming ability in Bacteroides fragilis strains isolated from patients diagnosed with colorectal cancer
ConclusionsBased on our findings, there was a difference between the abundance of patterns I, II, and III and biofilm formation in isolates obtained from CRC and normal tissues. Biofilm formation ability and toxin encoding gene (bft) are two main virulence factors inB. fragilis pathogenicity which require more investigation to treatB. fragilis infections effectively. (Source: Gut Pathogens)
Source: Gut Pathogens - June 4, 2020 Category: Microbiology Source Type: research
A novel 5-Plex qPCR-HRM assay detecting human diarrheal parasites
ConclusionsThe assay is a convenient and cost-effective method that could contribute to a quicker and accurate diagnosis as well as to more targeted therapies of parasite-derived diarrhea. Finally, this new multiplex PCR assay could also be instrumental in epidemiology studies on these parasites. (Source: Gut Pathogens)
Source: Gut Pathogens - May 28, 2020 Category: Microbiology Source Type: research
Clostridioides difficile ribotype distribution in a large teaching hospital in Serbia
ConclusionClostridium difficile ribotype 027 was the most prevalent ribotype among patients in a large Serbian hospital, but there is a clear decreasing trend. (Source: Gut Pathogens)
Source: Gut Pathogens - May 21, 2020 Category: Microbiology Source Type: research
Type IV secretion of Helicobacter pylori CagA into oral epithelial cells is prevented by the absence of CEACAM receptor expression
ConclusionsThese results show that lack of CEACAM receptors on the surface of the oral epithelial cells was responsible for resistance toH. pylori CagA-dependent pathogenic activities, and confirms the important role for the T4SS-dependent interaction of these receptors withH. pylori in the gastric epithelium. (Source: Gut Pathogens)
Source: Gut Pathogens - May 13, 2020 Category: Microbiology Source Type: research
Host responses to Clostridium perfringens challenge in a chicken model of chronic stress
ConclusionCORT administration modulated a number of host functions, which corresponded to increased densities ofC. perfringens in the small intestine and weight gain impairment in chickens. Importantly, results implicate physiological stress as an important predisposing factor to NE, which emphasizes the importance of managing stress to optimize chicken health. (Source: Gut Pathogens)
Source: Gut Pathogens - May 5, 2020 Category: Microbiology Source Type: research
Exosomal miRNAs in hepatitis B virus related liver disease: a new hope for biomarker
AbstractThe World Health Organisation, in its 2019 progress report on HIV, viral hepatitis and STDs indicates that 257 million people are afflicted with chronic HBV infections, of which, 1 million patients lose their lives every year due to HBV related chronic liver diseases including serious complications such as liver cirrhosis and hepatocellular carcinoma. The course of HBV infection and associated liver injury depend on several host factors, genetic variability of the virus, and the host viral interplay. The challenge of medical science is the early diagnosis/identification of the potential for development of fatal com...
Source: Gut Pathogens - April 23, 2020 Category: Microbiology Source Type: research
EV71 virus reduces Nrf2 activation to promote production of reactive oxygen species in infected cells
ConclusionsEV71 infection can trigger ROS production, cell death, and inflammatory reactions by modulating the Nrf2 and Keap1 levels of infected cells. (Source: Gut Pathogens)
Source: Gut Pathogens - April 22, 2020 Category: Microbiology Source Type: research
Prevalence and antimicrobial susceptibility of Arcobacter species in human stool samples derived from out- and inpatients: the prospective German Arcobacter prevalence study Arcopath
ConclusionsIn summary,Arcobacter includingA. butzleri,A. cryaerophilus andA. lanthieri could be isolated in 0.85% of German outpatients and ciprofloxacin rather than other antibiotics might be appropriate for antibiotic treatment of infections. Further epidemiological studies are needed, however, to provide a sufficient risk assessment ofArcobacter infections in humans. (Source: Gut Pathogens)
Source: Gut Pathogens - April 14, 2020 Category: Microbiology Source Type: research
Antibacterial activity of ethoxzolamide against Helicobacter pylori strains SS1 and 26695
AbstractWith the rise of bacterial resistance to conventional antibiotics, re-purposing of Food and Drug Administration (FDA) approved drugs currently used to treat non-bacteria related diseases as new leads for antibacterial drug discovery has become an attractive alternative. Ethoxzolamide (EZA), an FDA-approved diuretic acting as a human carbonic anhydrase inhibitor, is known to kill the gastric pathogenic bacteriumHelicobacter pylori in vitro via an, as yet, unknown mechanism. To date, EZA activity and resistance have been investigated for only oneH. pylori strain, P12. We have now performed a susceptibility and resist...
Source: Gut Pathogens - April 14, 2020 Category: Microbiology Source Type: research
