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Isobavachalcone exhibits antifungal and antibiofilm effects against C. albicans by disrupting cell wall/membrane integrity and inducing apoptosis and autophagy
This study aimed to evaluate the efficacy of IBC against planktonic growth and biofilms of Candida albicans (C. albicans) and the mechanisms underlying its antifungal action. The cell membrane integrity, cell metabolic viability, and cell morphology of C. albicans treated with IBC were evaluated using CLSM and FESEM analyses. Crystal violet staining, CLSM, and FESEM were used to assess the inhibition of biofilm formation, as well as dispersal and killing effects of IBC on mature biofilms. RNA-seq combined with apoptosis and autophagy assays was used to examine the mechanisms underlying the antifungal action of IBC. IBC exh...
Source: Frontiers in cellular and infection microbiology - January 22, 2024 Category: Microbiology Source Type: research

The pediatric gut bacteriome and virome in response to SARS-CoV-2 infection
DiscussionTo our knowledge, this is the first study to address the bacteriome, virome, and resistome of pediatric patients in response to COVID-19 and to preventative antibiotics use. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 22, 2024 Category: Microbiology Source Type: research

Red recombination enables a wide variety of markerless manipulation of porcine epidemic diarrhea virus genome to generate recombinant virus
Porcine epidemic diarrhea virus (PEDV) is a member of the genera Alphacoronavirus that has been associated with acute watery diarrhea and vomiting in swine. Unfortunately, no effective vaccines and antiviral drugs for PEDV are currently available. Reverse genetics systems are crucial tools for these researches. Here, a PEDV full-length cDNA clone was constructed. Furtherly, three PEDV reporter virus plasmids containing red fluorescent protein (RFP), Nano luciferase (Nluc), or green fluorescence protein (GFP) were generated using Red recombination with the GS1783 E. coli strain. These reporter-expressing recombinant (r) PED...
Source: Frontiers in cellular and infection microbiology - January 22, 2024 Category: Microbiology Source Type: research

Invasive Streptococcus suis isolated in Spain contain a highly promiscuous and dynamic resistome
ConclusionsOur work evidences that S. suis is a major contributor to AMR dissemination across veterinary and human pathogens. Therefore, control of AMR in S. suis should be considered from a One Health approach in regions with high pig production to properly tackle the issue of antimicrobial drug resistance. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 22, 2024 Category: Microbiology Source Type: research

Clinical, epidemiological, and drug resistance insights into HIV-positive patients in Meizhou, China
This study undertakes a retrospective analysis of the epidemiological and clinical features of 195 HIV-positive cases in Meizhou, China, from May 1, 2018 to December 31, 2019. Western blotting (WB) confirmed and assessed these cases. Notably, the majority of cases emanated from socio-economic groups with comparatively lower levels of education, with 80% being male. Strikingly, 90% of the cases were found to be in the middle to late stages of infection based on CD4+ T cell counts. Among the 30 different serum antibody profiles examined, reactivity with seven bands (p24, p31, gp41, p51, p66, gp120, and gp160) emerged as the ...
Source: Frontiers in cellular and infection microbiology - January 19, 2024 Category: Microbiology Source Type: research

Strategies for combating antibiotic resistance in bacterial biofilms
This article provides a contemporary assessment of biofilms and their treatment, with a focus on recent advances, to chronicle the evolving landscape of biofilm research. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 19, 2024 Category: Microbiology Source Type: research

Identification of apoptosis-related gene signatures as potential biomarkers for differentiating active from latent tuberculosis via bioinformatics analysis
ConclusionThe six ARGs can serve as effective biomarkers differentiating ATB from LTBI and provide insight into the pathogenesis of Mycobacterium tuberculosis infection. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 19, 2024 Category: Microbiology Source Type: research

Microbiomic signatures of anal fistula and putative sources of microbes
This study found that microbes in anal fistulas may originate from the skin surface in addition to the intestinal tract. This finding was further validated by NMDS analysis, which also indicated that the microbial communities in the inner and outer openings of the fistula were more similar to their surrounding environments. Using MaAslin2, the characteristics of the microbiome were examined, demonstrating a higher similarity between the abundant bacteria in the anal fistula samples and those found on the skin surface. Moreover, pin-to-pair analysis conducted on all subjects consistently showed a higher abundance of skin-so...
Source: Frontiers in cellular and infection microbiology - January 19, 2024 Category: Microbiology Source Type: research

Gut colonization and subsequent infection of neonates caused by extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae
The gut microbiota harbors diverse bacteria considered reservoirs for antimicrobial resistance genes. The global emergence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales (ESBL-PE) significantly contributes to healthcare-associated infections (HAIs). We investigated the presence of ESBL-producing Escherichia coli (ESBL-PEco) and ESBL-producing Klebsiella pneumoniae (ESBL-PKpn) in neonatal patients’ guts. Furthermore, we identified the factors contributing to the transition towards ESBL-PEco and ESBL-PKpn-associated healthcare-associated infections (HAIs). The study was conducted from August 2019 to ...
Source: Frontiers in cellular and infection microbiology - January 19, 2024 Category: Microbiology Source Type: research

Editorial: Pathogenesis, diagnostics, treatments of Mycobacterium tuberculosis and its co-infection with HIV or SARS-CoV-2
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 18, 2024 Category: Microbiology Source Type: research

Editorial: Enterobacteriaceae antimicrobial agents and resistance: relationship with the therapeutic approach, volume II
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 18, 2024 Category: Microbiology Source Type: research

Characterization of viral infections in children with influenza-like-illness during December 2018 –January 2019
IntroductionRespiratory viral infection (RVI) is of very concern after the outbreak of COVID-19, especially in pediatric departments. Learning pathogen spectrum of RVI in children previous the epidemic of COVID-19 could provide another perspective for understanding RVI under current situation and help to prepare for the post COVID-19 infection control.MethodsA nucleic acid sequence-based amplification (NASBA) assay, with 19 pairs of primers targeting various respiratory viruses, was used for multi-pathogen screening of viral infections in children presenting influenza-like illness (ILI) symptoms. Children with ILI at the o...
Source: Frontiers in cellular and infection microbiology - January 18, 2024 Category: Microbiology Source Type: research

Gut microbiota and its metabolites in non-small cell lung cancer and brain metastasis: from alteration to potential microbial markers and drug targets
ConclusionCompared with healthy controls, distinct host-microbe interactions were evident in different phases of patients with NSCLC. Furthermore, specific forms of the gut microbiome and SCFAs may serve as valuable biomarkers and therapeutic targets in the diagnosis and treatment of NSCLC. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 18, 2024 Category: Microbiology Source Type: research

Editorial: Early events during host cell-pathogen interaction, volume II
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 17, 2024 Category: Microbiology Source Type: research

A review of neuro-ophthalmic sequelae following COVID-19 infection and vaccination
ConclusionsThe various COVID-19 vaccines that are currently available are part of the global effort to protect the most vulnerable of the human population. The incidence of neuro-ophthalmic consequences following infection with COVID-19 is hundred-folds higher and associated with more harrowing systemic effects than vaccination against the virus. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - January 17, 2024 Category: Microbiology Source Type: research