Characterization of the B-Cell Epitopes of Echinococcus granulosus Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts
Cystic echinococcosis (CE) is a zoonotic disease worldwide distributed, caused by the cestode Echinococcus granulosus sensu lato (E. granulosus), with an incidence rate of 50/100,000 person/year and a high prevalence in humans of 5-10%. Serology has variable sensitivity and specificity and low predictive values. Antigens used are from the hydatid fluid and recombinant antigens have not demonstrated superiority over hydatid fluid. A cell line called EGPE was obtained from E. granulosus sensu lato G1 strain from bovine liver. Serum from CE patients recognizes protein extracts from EGPE cells with higher sensitivity than prot...
Source: Frontiers in cellular and infection microbiology - June 13, 2022 Category: Microbiology Source Type: research

Dynamic Python-Based Method Provides Quantitative Analysis of Intercellular Junction Organization During S. pneumoniae Infection of the Respiratory Epithelium
Many respiratory pathogens compromise epithelial barrier function during lung infection by disrupting intercellular junctions, such as adherens junctions and tight junctions, that maintain intercellular integrity. This includes Streptococcus pneumoniae, a leading cause of pneumonia, which can successfully breach the epithelial barrier and cause severe infections such as septicemia and meningitis. Fluorescence microscopy analysis on intercellular junction protein manipulation by respiratory pathogens has yielded major advances in our understanding of their pathogenesis. Unfortunately, a lack of automated image analysis tool...
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

Editorial: On the Inflammatory Cascade-From Bacteria Through the Epithelium to the Connective Tissue
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

Flavorubredoxin, a Candidate Trigger Related to Thrombotic Thrombocytopenic Purpura: Screening of the Complete Genome of a Salmonella enterica Serovar Typhimurium Isolate From an AIDS Case
Thrombotic thrombocytopenic purpura (TTP) is one of the two classic thrombotic microangiopathy (TMA) diseases which could be induced by infections. To the best of our knowledge, this is the first report of an acquired immunodeficiency syndrome (AIDS) patient with acquired TTP induced by infection with Salmonella enterica serovar Typhimurium (hereafter, S. Typhimurium) isolate, S. Typhimurium_zhang, which was confirmed by serology and genetic taxonomy. The literature review identified 17 TMA-related genes encoding the candidate triggers, which were searched in the annotated genome sequence of S. Typhimurium_zhang. Anaerobic...
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

Evidence of Infection of Human Embryonic Stem Cells by SARS-CoV-2
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was initially described to target the respiratory system and now has been reported to infect a variety of cell types, including cardiomyocytes, neurons, hepatocytes, and gut enterocytes. However, it remains unclear whether the virus can directly infect human embryonic stem cells (hESCs) or early embryos. Herein, we sought to investigate this question in a cell-culture system of hESCs. Both the RNA and S protein of SARS-CoV-2 were detected in the infected hESCs and the formation of syncytium was observed. The increased level of subgenomic viral RNA and the pre...
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

The Effectiveness of Metagenomic Next-Generation Sequencing in the Diagnosis of Prosthetic Joint Infection: A Systematic Review and Meta-Analysis
ConclusionMetagenomic next-generation sequencing displays high accuracy in the diagnosis of PJI, especially for culture-negative cases. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

Labeling Strategies for Surface-Exposed Protein Visualization and Determination in Plasmodium falciparum Malaria
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

First Evidence of Entamoeba Parasites in Australian Wild Deer and Assessment of Transmission to Cattle
Australian wild deer populations have significantly expanded in size and distribution in recent decades. Due to their role in pathogen transmission, these deer populations pose a biosecurity risk to the livestock industry. However, little is known about the infection status of wild deer in Australia. The intestinal parasite Entamoeba bovis has been previously detected in farm and wild ruminants worldwide, but its epidemiology and distribution in wild ruminants remain largely unexplored. To investigate this knowledge gap, faecal samples of wild deer and domestic cattle from south-eastern Australia were collected and analyse...
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

New Procedure to Maintain Fecal Microbiota in a Dry Matrix Ready to Encapsulate
Fecal microbiota transplantation (FMT) is one of the recommended treatments for recurrent Clostridioides difficile infection, but endoscopy and available oral formulations still have several limitations in their preparation, storage, and administration. The need for a viable oral formulation that facilitates the implementation of this highly effective therapy in different settings has led us to test the microcrystalline cellulose particles as an adsorbent of concentrated filtered fresh feces in comparison to lyophilized feces. This free-flowing material can provide protection to bacteria and results in a dried product able...
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

Automated Machine Learning for the Early Prediction of the Severity of Acute Pancreatitis in Hospitals
ConclusionsThe AutoML model based on the GBM algorithm for early prediction of SAP showed evident clinical practicability. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

Usefulness of Random Forest Algorithm in Predicting Severe Acute Pancreatitis
ConclusionsAn interpretable RF model exhibited the highest discriminatory performance in predicting SAP. Interpretation with LIME plots could be useful for individualized prediction in a clinical setting. A nomogram consisting of albumin, serum creatinine, glucose, and pleural effusion was useful for prediction of SAP. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - June 10, 2022 Category: Microbiology Source Type: research

The Urobiomes of Adult Women With Various Lower Urinary Tract Symptoms Status Differ: A Re-Analysis
The discovery of the urinary microbiome (urobiome) has created opportunities for urinary health researchers who study a wide variety of human health conditions. This manuscript describes an analysis of catheterized urine samples obtained from 1,004 urobiome study participants with the goal of identifying the most abundant and/or prevalent (common) taxa in five clinically relevant cohorts: unaffected adult women (n=346, 34.6%), urgency urinary incontinence (UUI) (n=255, 25.5%), stress urinary incontinence (SUI) (n=50, 5.0%), urinary tract infection (UTI) (n=304, 30.4%), and interstitial cystitis/painful bladder syndrome (IC...
Source: Frontiers in cellular and infection microbiology - June 9, 2022 Category: Microbiology Source Type: research

The State-of-the-Art of Gene Editing and its Application to Viral Infections and Diseases Including COVID-19
Gene therapy delivers a promising hope to cure many diseases and defects. The discovery of gene-editing technology fueled the world with valuable tools that have been employed in various domains of science, medicine, and biotechnology. Multiple means of gene editing have been established, including CRISPR/Cas, ZFNs, and TALENs. These strategies are believed to help understand the biological mechanisms of disease progression. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been designated the causative virus for coronavirus disease 2019 (COVID-19) that emerged at the end of 2019. This viral infection is a h...
Source: Frontiers in cellular and infection microbiology - June 9, 2022 Category: Microbiology Source Type: research

Adapt or Die: Targeting Unique Transmission-Stage Biology for Malaria Elimination
Plasmodium parasites have a complex life cycle that includes development in the human host as well as the Anopheles vector. Successful transmission of the parasite between its host and vector therefore requires the parasite to balance its investments in asexual replication and sexual reproduction, varying the frequency of sexual commitment to persist within the human host and generate future opportunities for transmission. The transmission window is extended further by the ability of stage V gametocytes to circulate in peripheral blood for weeks, whereas immature stage I to IV gametocytes sequester in the bone marrow and s...
Source: Frontiers in cellular and infection microbiology - June 9, 2022 Category: Microbiology Source Type: research

Simultaneous Detection of Pathogens and Tumors in Patients With Suspected Infections by Next-Generation Sequencing
ConclusionThis preliminary study demonstrates the feasibility and clinical value of using Onco-mNGS to simultaneously search for potential pathogens and malignant tumors in patients with suspected infections. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - June 9, 2022 Category: Microbiology Source Type: research