Transcriptomic changes in the large organs in lethal meningococcal shock are reflected in a porcine shock model
ConclusionThe inflammatory responses measured on the transcriptomic level in organs in patients with fulminant meningococcal sepsis is reproduced in the porcine model of the disease, although some differences may exist regarding the top-upregulated factors in individual organs. Thus, this large animal model reproduces important immunological features of meningococcal sepsis and can be a valuable tool in further investigations of inflammatory aspects and possible treatment options (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 11, 2022 Category: Microbiology Source Type: research

Saliva microbiome changes in thyroid cancer and thyroid nodules patients
ConclusionThe salivary microbiota variation may be connected with thyroid cancer and thyroid nodules. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 11, 2022 Category: Microbiology Source Type: research

Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin
In this study, pleural thickening was discovered to be associated with increased collagen fibers and elastic fibers. Furthermore, collagen-I and elastin were found to be up-regulated and concentrated in the pleura at the mRNA and protein levels following infection. To summarize, our findings add to the theoretical understanding of Glässer’s disease and provide strong support for further research into the pathogenic mechanism of Glaesserella parasuis and the program’s target treatment. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 11, 2022 Category: Microbiology Source Type: research

Novel highly-multiplexed AmpliSeq targeted assay for Plasmodium vivax genetic surveillance use cases at multiple geographical scales
Although the power of genetic surveillance tools has been acknowledged widely, there is an urgent need in malaria endemic countries for feasible and cost-effective tools to implement in national malaria control programs (NMCPs) that can generate evidence to guide malaria control and elimination strategies, especially in the case of Plasmodium vivax. Several genetic surveillance applications (‘use cases’) have been identified to align research, technology development, and public health efforts, requiring different types of molecular markers. Here we present a new highly-multiplexed deep sequencing assay (Pv AmpliSeq). T...
Source: Frontiers in cellular and infection microbiology - August 11, 2022 Category: Microbiology Source Type: research

Identification of a non-canonical G3BP-binding sequence in a Mayaro virus nsP3 hypervariable domain
We report that G3BP1 and G3BP2 are both required for MAYV growth in human cells and bind nsP3 protein. In infected cells, they are recruited to nsP3-containing cytosolic foci and active replication complexes. Unexpectedly, deletion of the single FGxF sequence in MAYV nsP3 did not abolish these phenotypes. Using mutagenesis and in silico modeling, we identify an upstream FGAP amino acid sequence as an additional MAYV nsP3/G3BP interaction motif required for optimal viral infectivity. These results, therefore, highlight a non-conventional G3BP binding sequence in MAYV nsP3. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 11, 2022 Category: Microbiology Source Type: research

Analysis of gene expression profile of peripheral blood in alveolar and cystic echinococcosis
In this study, peripheral blood samples were collected from six patients with alveolar echinococcosis (AE), six patients with cystic echinococcosis (CE), and six healthy controls. RNA-Seq (mRNA) was performed to detect gene transcript and expression levels, and DEGs were subjected to bioinformatic analyses. In comparison with healthy controls, 492 DEGs (270 upregulated, 222 downregulated) were found in the AE group and 424 DEGs (170 upregulated, 254 downregulated) were found in the CE group (|log2 (fold change)| > 1 and P < 0.05). Further, 60 genes were upregulated and 39 were downregulated in both the AE and CE grou...
Source: Frontiers in cellular and infection microbiology - August 11, 2022 Category: Microbiology Source Type: research

Characteristics of oral microbiota in plateau and plain youth ‐positive correlations between blood lipid level, metabolism and specific microflora in the plateau group
ConclusionsWith increasing altitude, the diversity of oral microbiota and the relative proportion of predominant bacteria were altered. The distribution and related function of Streptococcus spp. were prominent in plateau samples. This comprehensive study of the relationship between oral microecology and elevation provides a point of reference for studying the human body’s adaptability or inadaptability to high altitude. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

Curcumin-driven reprogramming of the gut microbiota and metabolome ameliorates motor deficits and neuroinflammation in a mouse model of Parkinson ’s disease
(Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

Differentiation of otitis media-causing bacteria and biofilms via Raman spectroscopy and optical coherence tomography
This study proposes the combination of OCT and Raman spectroscopy (RS) to examine differences in the refractive index, optical attenuation, and biochemical composition of Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, and Pseudomonas aeruginosa; four of the leading otopathogens in OM. This combination provides a dual optical approach for identifying and differentiating OM-causing bacterial species under three different in vitro growth environments (i.e., agar-grown colonies, planktonic cells from liquid cultures, and biofilms). This study showed that RS was able to identify key biochemical variati...
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

Porcisia transmission by prediuresis of sand flies
In conclusion, we demonstrated the vector competence of L. longipalpis for P. deanei but not for P. hertigi, and further studies are needed to evaluate competence of other Neotropical vectors for these neglected parasites. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

Gut microbiota remodeling: A promising therapeutic strategy to confront hyperuricemia and gout
The incidence of hyperuricemia (HUA) and gout continuously increases and has become a major public health problem. The gut microbiota, which colonizes the human intestine, has a mutually beneficial and symbiotic relationship with the host and plays a vital role in the host’s metabolism and immune regulation. Structural changes or imbalance in the gut microbiota could cause metabolic disorders and participate in the synthesis of purine-metabolizing enzymes and the release of inflammatory cytokines, which is closely related to the occurrence and development of the metabolic immune disease HUA and gout. The gut microbiota a...
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

Exploring the mechanism of action of Xuanfei Baidu granule (XFBD) in the treatment of COVID-19 based on molecular docking and molecular dynamics
ConclusionFor the first time, it was found that the important active chemical components in XFBD, such as I-SPD, Pachypodol and Vestitol, reduce inflammatory response and apoptosis by inhibiting the activation of NLRP3, and reduce the production of inflammatory factors and chemotaxis of inflammatory cells by inhibiting the activation of CSF2. Therefore, XFBD can effectively alleviate the clinical symptoms of COVID-19 through NLRP3 and CSF2. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

Reduced diversity of intestinal T-cell receptor repertoire in patients with Crohn ’s disease
ConclusionsThe diversity of the TCR repertoire is reduced in inflamed mucosa of CD patients, which might contribute to intestinal inflammation. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

Where do T cell subsets stand in SARS-CoV-2 infection: an update
An outbreak of coronavirus disease 2019 (COVID-19) emerged in China in December 2019 and spread so rapidly all around the globe. It’s continued and spreading more dangerously in India and Brazil with higher mortality rate. Understanding of the pathophysiology of COVID-19 depends on unraveling of interactional mechanism of SARS-CoV-2 and human immune response. The immune response is a complex process, which can be better understood by understanding the immunological response and pathological mechanisms of COVID-19, which will provide new treatments, increase treatment efficacy, and decrease mortality associated with the d...
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research

How concerning is a SARS-CoV-2 variant of concern? Computational predictions and the variants labeling system
In this study, we evaluated the use of a predictive computational approach for SARS-CoV-2 genetic variations analysis in improving the current variant labeling system. First, we reviewed the basis of the system developed by the World Health Organization (WHO) for the labeling of SARS-CoV-2 genetic variants and the derivative adapted by the United States Centers for Disease Control and Prevention (CDC). Both labeling systems are based on the virus’ major attributes. However, we found that the labeling criteria of the SARS-CoV-2 variants derived from these attributes are not accurately defined and are used differently by t...
Source: Frontiers in cellular and infection microbiology - August 10, 2022 Category: Microbiology Source Type: research