Developmental and Comparative Immunology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Prokaryotic and eukaryotic dual-expression plasmid-mediated delivery of Campylobacter jejuni antigens by live-attenuated Salmonella: A strategy for concurrent Th1 and Th2 immune activation and protection in chickens
Dev Comp Immunol. 2024 Apr;153:105134. doi: 10.1016/j.dci.2024.105134. Epub 2024 Jan 6.ABSTRACTSalmonella and Campylobacter are food-borne pathogens that significantly affect poultry production and are transmitted to humans. Long-term protection against these pathogens in chicken relies on a balanced Th1 and Th2 response. C. jejuni antigens were screened and a fusion antigen, including CadF + FlaA adhesin and flagellin antigenic fragments was developed and safely delivered by low-endotoxicity S. Typhimurium through pJHL270, a dual-expression plasmid featuring prokaryotic (Ptrc) and eukaryotic (CMV) promoters. Antigen expre...
Source: Developmental and Comparative Immunology - January 8, 2024 Category: Allergy & Immunology Authors: Sivasankar Chandran Chamith Hewawaduge Ram Prasad Aganja John Hwa Lee Source Type: research

Prokaryotic and eukaryotic dual-expression plasmid-mediated delivery of Campylobacter jejuni antigens by live-attenuated Salmonella: A strategy for concurrent th1 and th2 immune activation and protection in chickens
Dev Comp Immunol. 2024 Jan 6:105134. doi: 10.1016/j.dci.2024.105134. Online ahead of print.ABSTRACTSalmonella and Campylobacter are food-borne pathogens that significantly affect poultry production and are transmitted to humans. Long-term protection against these pathogens in chicken relies on a balanced Th1 and Th2 response. C. jejuni antigens were screened and a fusion antigen, including CadF + FlaA adhesin and flagellin antigenic fragments was developed and safely delivered by low-endotoxicity S. Typhimurium through pJHL270, a dual-expression plasmid featuring prokaryotic (Ptrc) and eukaryotic (CMV) promoters. Antigen e...
Source: Developmental and Comparative Immunology - January 8, 2024 Category: Allergy & Immunology Authors: Sivasankar Chandran Chamith Hewawaduge Ram Prasad Aganja John Hwa Lee Source Type: research

The effects of primary and secondary bacterial exposure on the seahorse (Hippocampus erectus) immune response
Dev Comp Immunol. 2024 Jan 5:105136. doi: 10.1016/j.dci.2024.105136. Online ahead of print.ABSTRACTEvolutionary adaptations in the Syngnathidae teleost family (seahorses, pipefish and seadragons) culminated in an array of spectacular morphologies, key immune gene losses, and the enigmatic male pregnancy. In seahorses, genome modifications associated with immunoglobulins, complement, and major histocompatibility complex (MHC II) pathway components raise questions concerning their immunological efficiency and the evolution of compensatory measures that may act in their place. In this investigation heat-killed bacteria (Vibri...
Source: Developmental and Comparative Immunology - January 7, 2024 Category: Allergy & Immunology Authors: Jamie Parker Silke-Mareike Marten Tadhg C Ó Corcora Jelena Rajkov Arseny Dubin Olivia Roth Source Type: research

Uncovering the antimicrobial activity of G-type lysozyme 2 derived from Cyprinus carpio mucus against bacterial and fungal pathogens
This study aimed to explore the antimicrobic potential of mucus samples collected from Cyprinus carpio and identify the specific antimicrobial peptides responsible for its activity. The crude extract was tested against various bacterial and fungal pathogens, and its protein content and profile were analyzed. Purification steps, including gel filtration chromatography, were employed to isolate the most active fraction (peak IV), which was further identified via liquid chromatography and mass spectroscopy. The results revealed varying degrees of antimicrobial activity of the crude extract against different bacterial and fung...
Source: Developmental and Comparative Immunology - January 7, 2024 Category: Allergy & Immunology Authors: Uzma Shabir Jehangir Shafi Dar Aashaq Hussain Bhat Bashir Ahmad Ganai Mohamed H Mahmoud Gaber El-Saber Batiha Source Type: research

A single coelomic cell type is involved in both immune and respiratory functions of the coastal bioindicator annelid: Capitella C-Channel1 from the English Channel
Dev Comp Immunol. 2024 Jan 4;153:105132. doi: 10.1016/j.dci.2024.105132. Online ahead of print.ABSTRACTThe polychaete Capitella is a typical member of the 'thiobiome', and is commonly used as an eutrophication indicator species in environmental assessment studies. To deal with a sulfide-rich and poisonous surrounding, cells in close contact with the environment, and thus able to play a major role in detoxication and survival, are circulating cells. This work aimed to morpho-functionally describe the circulating coelomic cells of Capitella from the English Channel inhabiting the sulfide-rich mud in Roscoff Harbor. In genera...
Source: Developmental and Comparative Immunology - January 5, 2024 Category: Allergy & Immunology Authors: C éline Boidin-Wichlacz Ann C Andersen Nathalie Jouy St éphane Hourdez Aur élie Tasiemski Source Type: research

A single coelomic cell type is involved in both immune and respiratory functions of the coastal bioindicator annelid: Capitella C-Channel1 from the English Channel
Dev Comp Immunol. 2024 Jan 3:105132. doi: 10.1016/j.dci.2024.105132. Online ahead of print.ABSTRACTThe polychaete Capitella is a typical member of the 'thiobiome', and is commonly used as an eutrophication indicator species in environmental assessment studies. To deal with a sulfide-rich and poisonous surrounding, cells in close contact with the environment, and thus able to play a major role in detoxication and survival, are circulating cells. This work aimed to morpho-functionally describe the circulating coelomic cells of Capitella from the English Channel inhabiting the sulfide-rich mud in Roscoff Harbor. In general, w...
Source: Developmental and Comparative Immunology - January 5, 2024 Category: Allergy & Immunology Authors: C éline Boidin-Wichlacz Ann C Andersen Nathalie Jouy St éphane Hourdez Aur élie Tasiemski Source Type: research

PyLKB1 regulates glucose transport via activating PyAMPK α in Yesso Scallop Patinopecten yessoensis under high temperature stress
In this study, a homologous molecule of LKB1 with a typical serine/threonine kinase domain and two nuclear localization sequences (NLSs) was identified in Yesso Scallop Patinopecten yessoensis (PyLKB1). The mRNA transcripts of PyLKB1 were found to be expressed in haemocytes and all the examined tissues, including gill, mantle, gonad, adductor muscle and hepatopancreas, with the highest expression level in hepatopancreas. PyLKB1 was mainly located in cytoplasm and nucleus of scallop haemocytes. At 3 h after high temperature stress treatment (25 °C), the mRNA transcripts of PyLKB1, PyAMPKα, and PyGLUT1 in hepatopancreas, t...
Source: Developmental and Comparative Immunology - January 1, 2024 Category: Allergy & Immunology Authors: Dongli Jiang Chuanyan Yang Wenfei Gu Xiaoxue Ma Ziling Tong Lingling Wang Linsheng Song Source Type: research

HIF-1-mediated regulation of LDH gene unravels key insights into MCDV-1 pathogenesis in mud crabs Scylla paramamosain
In this study, the intricate interplay between HIF-1 and the key glycolysis enzyme, lactate dehydrogenase (LDH), was investigated after MCDV-1 infection. The expression of LDH was significant increased after MCDV-1 infection. Additionally, the expression of HIF-1α was upregulated following MCDV-1 infection, potentially attributed to the downregulation of prolyl hydroxylase domains 2 expression. Subsequent examination of the SpLDH promoter identified the presence of hypoxia response elements (HREs), serving as binding sites for HIF-1α. Intriguingly, experimental evidence demonstrated that SpHIF-1α actively promotes SpLDH...
Source: Developmental and Comparative Immunology - December 31, 2023 Category: Allergy & Immunology Authors: Yu-Kun Jie Jia-Wei Xie Chang-Hong Cheng Jian-Jun Jiang Hong-Ling Ma Guang-Xin Liu Si-Gang Fan Yi-Qin Deng Juan Feng Zhi-Xun Guo Source Type: research

Structural and pharmacological characterization of a medium-chain fatty acid receptor GPR84 in common carp (Cyprinus carpio)
In this study, we cloned the coding sequence (CDS) of common carp GPR84 (ccGPR84), examined its tissue distribution, and explored its cellular signaling function. The results showed that the CDS of ccGPR84 is 1191 bp and encodes a putative protein with 396 amino acids. Phylogenetic and chromosomal synteny analyses revealed that ccGPR84 was evolutionarily conserved with Cyprinids. Real-time quantitative PCR (qPCR) indicated that ccGPR84 was predominantly expressed in the intestine and spleen. Luciferase reporter assay demonstrated that nonanoic acid, capric acid (decanoic acid), undecanoic acid and lauric acid could inhibit...
Source: Developmental and Comparative Immunology - December 31, 2023 Category: Allergy & Immunology Authors: Ming-Xing Yao Hui-Xia Yu Hao-Lin Mo Zhi-Hao Zhang Qing-Chuan Song Qiao Liu Qi-Yuan Yang Li-Xin Wang Yang Li Source Type: research

Transcriptomic profiling and the first spatial expression analysis of candidate genes in the salivary gland of the East Asian medicinal leech, Hirudo nipponia
In this study, we conducted an extensive transcriptomic profiling of the salivary gland tissue in H. nipponia based on de novo assemblies of tissue-specific transcriptomes from the salivary gland, teeth, and general head region. Through gene ontology (GO) analysis and hierarchical clustering, we discovered a novel set of anti-coagulant factors-i.e., Hni-Antistasin, Hni-Ghilanten, Hni-Bdellin, Hni-Hirudin-as well as a previously unrecognized immune-related gene, Hni-GLIPR1 and uncharacterized salivary gland specific transcripts. By employing in situ hybridization, we provided the first visualization of gene expression sites...
Source: Developmental and Comparative Immunology - December 29, 2023 Category: Allergy & Immunology Authors: Jung-Hyeuk Kim Chan-Jun Lee Yun-Sang Yu Yam Prasad Aryal Sangil Kim Jo-Young Suh Jae-Young Kim Sun Hong Min In-Tae Cha Hae-Youn Lee Song Yub Shin Sung-Jin Cho Source Type: research

Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass
Dev Comp Immunol. 2023 Dec 23;152:105124. doi: 10.1016/j.dci.2023.105124. Online ahead of print.ABSTRACTCell-mediated cytotoxicity (CMC) is essential in eradicating virus-infected cells, involving CD8+ T lymphocytes (CTLs) and natural killer (NK) cells, through the activation of different pathways. This immune response is well-studied in mammals but scarcely in teleost fish. Our aim was to investigate the adaptive CMC using head-kidney (HK) cells from European sea bass infected at different times with nodavirus (NNV), as effector cells, and the European sea bass brain cell line (DLB-1) infected with different NNV genotypes...
Source: Developmental and Comparative Immunology - December 25, 2023 Category: Allergy & Immunology Authors: Miguel A Garc ía-Álvarez Elena Chaves-Pozo Alberto Cuesta Source Type: research

Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass
Dev Comp Immunol. 2023 Dec 23:105124. doi: 10.1016/j.dci.2023.105124. Online ahead of print.ABSTRACTCell-mediated cytotoxicity (CMC) is essential in eradicating virus-infected cells, involving CD8+ T lymphocytes (CTLs) and natural killer (NK) cells, through the activation of different pathways. This immune response is well-studied in mammals but scarcely in teleost fish. Our aim was to investigate the adaptive CMC using head-kidney (HK) cells from European sea bass infected at different times with nodavirus (NNV), as effector cells, and the European sea bass brain cell line (DLB-1) infected with different NNV genotypes, as...
Source: Developmental and Comparative Immunology - December 25, 2023 Category: Allergy & Immunology Authors: Miguel A Garc ía-Álvarez Elena Chaves-Pozo Alberto Cuesta Source Type: research