Developmental and Comparative Immunology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Identification and structural analysis of dimeric chicken complement component 3d and its binding with chicken complement receptor 2
In this study, we aimed to explored whether chicken C3d (chC3d) exhibits similar characteristics, namely dimerization and binding of dimeric chC3d to chicken CR2 (chCR2). We investigated the interaction and co-localization of chC3d with itself using coimmunoprecipitation and confocal laser scanning microscopy, respectively. Then, dimeric chC3d was detected using native polyacrylamide gel electrophoresis and western blotting, and its equilibrium dissociation constant KD (827 nM) was determined using surface plasmon resonance. Finally, the interaction modes of dimeric chC3d were identified using molecular docking simulations...
Source: Developmental and Comparative Immunology - December 7, 2023 Category: Allergy & Immunology Authors: Huan Jin Min Tu Zhaoying Meng Bo Jiang Qianqian Yang Yongqing Li Zhenhua Zhang Source Type: research

Congenital asplenia impairs heme-iron recycling during erythropoiesis in zebrafish
In this study, HSC-regulated and erythrocyte marker genes, such as gata1a, gata2, klf1, hbaa1, hbaa2, hbba1 and hbba2 were significantly reduced in congenitally asplenic zebrafish when compared with wild-type (WT). Subsequently, we conducted the transcriptome profiles of whole kidneys from WT and congenitally asplenic zebrafish to explore the possible molecular mechanisms underlying the impaired erythropoiesis caused by congenital asplenia. Our results demonstrated that congenital asplenia might impair heme-iron recycling during erythropoiesis, as evidenced by significant down-regulation of genes associated with iron acqui...
Source: Developmental and Comparative Immunology - December 1, 2023 Category: Allergy & Immunology Authors: Lang Xie Yixi Tao Ziwei Shen Huatang Deng Xinbin Duan Yang Xue Daqing Chen Yun Li Source Type: research

Short peptidoglycan recognition protein 5 modulates immune response to bacteria in Indian major carp, Cirrhinusmrigala
Dev Comp Immunol. 2023 Nov 29:105104. doi: 10.1016/j.dci.2023.105104. Online ahead of print.ABSTRACTPeptidoglycan recognition proteins (PGRPs) function in host antibacterial responses by recognizing bacterial peptidoglycan (PGN). In the present study, a short pgrp5 (named mpgrp5) was identified in Cirrhinus mrigala (mrigal). The full-length cDNA of the mpgrp5 gene was 1255 bp, containing an open reading frame of 746 bp encoding a protein of 248 amino acids. The predicted protein contained the typical Pgrp/amidase domain, conserved Zn2+, and PGN binding residues. The phylogenetic analysis revealed that the mpgrp5 is closely...
Source: Developmental and Comparative Immunology - December 1, 2023 Category: Allergy & Immunology Authors: Zahoor Mushtaq Pani Prasad Kurcheti K Jeena P Gireesh-Babu Source Type: research

DNase I rescues goat sperm entrapped by neutrophil extracellular traps via the NADPH oxidase, ERK < sub > 1/2 < /sub > , p38 signaling pathways and glycolysis
This study investigated the mechanism of sperm-induced NETs and further explored the impact of DNase I on NETs. Sperm quality was evaluated using optical microscopy, while the structure of NETs was observed through immunofluorescence staining. The formation mechanism of NETs was examined using inhibitors and PicoGreen. The findings revealed that sperm induced the formation of NETs, a process regulated by glycolysis, NADPH oxidase, ERK1/2, and p38 signaling pathways. The composition of NETs encompassed DNA, citrullinated histone H3 (citH3), and elastase (NE). DNase I protects sperm by degrading NETs, thereby concurrently pr...
Source: Developmental and Comparative Immunology - November 30, 2023 Category: Allergy & Immunology Authors: Zhengkai Wei Hongrong Hong Wei Liu Liqiang Jiang Jingnan Xu Xinxin Gao Yuxiao Qian Yuqian Jiang Zha Jin Qinqin Jin Meiyi Chen Zhengtao Yang Source Type: research

The identification of regulatory ceRNA network involved in Drosophila Toll immune responses
This study provides evidence of a ceRNA regulatory network in Drosophila Toll immune responses and offers novel insights into understanding the regulatory networks involved in the innate immunity of other animals.PMID:38013113 | DOI:10.1016/j.dci.2023.105105 (Source: Developmental and Comparative Immunology)
Source: Developmental and Comparative Immunology - November 28, 2023 Category: Allergy & Immunology Authors: Yu Huang Yujia Pang Yina Xu Li Liu Hongjian Zhou Source Type: research