Developmental and Comparative Immunology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Animal granulins: In the GRN scheme of things
Dev Comp Immunol. 2023 Dec 13:105115. doi: 10.1016/j.dci.2023.105115. Online ahead of print.ABSTRACTGranulins are conserved in nearly all metazoans, with an intriguing loss in insects. These pleiotropic peptides are involved in numerous physiological and pathological processes yet have been overwhelmingly examined in mammalian systems. While work in other animal models has been informative, a richer understanding of the proteins should be obtained by integrating knowledge from all available contexts. The main bodies of work described here include 1) the structure-function relationships of progranulin and its cleavage produ...
Source: Developmental and Comparative Immunology - December 15, 2023 Category: Allergy & Immunology Authors: Christina R Bowhay Patrick C Hanington Source Type: research

Ras3 in Bombyx mori with antiviral function against B. mori nucleopolyhedrovirus
In this study, we found that the overexpression of BmRas3 inhibited the infection of BmNPV(B. mori nucleopolyhedrovirus), while knockdown of BmRas3 could promote the infection of BmNPV. In addition, after the BmRas3 in silkworm larvae was knockdown, the anti-BmNPV ability of silkworm decreased and the survival rate of silkworm was affected. Additionly in the cells with BmRas3 overexpression, the transcription level of BmMapkk6 、BmP38、BmJNK、BmERK1/2 and BmERK5 were significantly increased after BmNPV infection, and the transcript levels of BmMapkk6、BmP38、BmJNK、BmERK1/2 and BmERK5 were also inhibited to varying d...
Source: Developmental and Comparative Immunology - December 15, 2023 Category: Allergy & Immunology Authors: Dingguo Xia Jiang Dan Pengcheng Yu Kaifang Jia Jinyang Wang Dongxu Shen Qiaoling Zhao Cheng Lu Source Type: research

Dual oxidase 2 (duox 2) participates in the intestinal antibacterial innate immune responses of Procambarus clarkii by regulating ROS levels
In this study, a duox 2 involved in the production of H2O2 was identified for the first time in P. clarkii. Mature Pc-Duox 2 is a 7-transmembrane protein molecule that includes PHD, FAD, and NAD domains. Pc-duox 2 was mainly expressed in hemocytes and intestinal tissue. Its expression levels were obviously upregulated after intramuscular or oral infection with V. harveyi. In the RNAi assay, the upregulated trends of H2O2 and total ROS levels in crayfish intestine were significantly suppressed when Pc-duox 2 was knocked down. Compared with the slightly affected SOD activity, the upregulated CAT activity was suppressed more ...
Source: Developmental and Comparative Immunology - December 15, 2023 Category: Allergy & Immunology Authors: Qianqian Li Mingda Zhang Shiyu Qin Jing Wen Xiuli Shen Zhiqiang Du Source Type: research

Innate immunity response of zafirlukast treated-tilapia during foreign body inflammation
Dev Comp Immunol. 2023 Dec 11:105112. doi: 10.1016/j.dci.2023.105112. Online ahead of print.ABSTRACTThere is limited knowledge regarding the blockade of cysteinyl leukotriene receptors (CysLTRs) and their effects in teleost fish. The present study investigated the effects of Zafirlukast, antagonist of CysLTR1 receptor, on the foreign body inflammatory reaction in Nile tilapia (Oreochromis niloticus). Zafirlukast-treated tilapia demonstrated a decrease in the formation of multinucleated foreign body giant cells and Langhans cells on the round glass coverslips implanted in the subcutaneous tissue, along with a significant re...
Source: Developmental and Comparative Immunology - December 13, 2023 Category: Allergy & Immunology Authors: Susana Luporini de Oliveira Camila Carlino da Costa Mayumi Fernanda Aracati Leticia Franchin Rodrigues Gabriel Conde Alessandra Cristina de Moraes Annelise Carla Camplesi Thais Heloisa Vaz Farias Ives Charlie Silva Luiz Arthur Malta Pereira Marco Antonio Source Type: research

Comparison of skin mucus lectins among longtooth grouper Epinephelus bruneus and giant grouper E. lanceolatus as well as the hybrid, Kue-Tama, and their binding abilities to the skin fluke Benedeniaepinepheli
Dev Comp Immunol. 2023 Dec 10:105113. doi: 10.1016/j.dci.2023.105113. Online ahead of print.ABSTRACTInterspecific hybrids of farm-raised fish are becoming popular in aquaculture owing to their advantages over pure species, including improved growth and higher resistance to infectious diseases. Kue-Tama is a recently established hybrid grouper derived from the longtooth grouper Epinephelus bruneus (♀) × giant grouper E. lanceolatus (♂). In our previous study, this hybrid showed significantly higher resistance against the skin fluke Benedenia epinepheli, a problematic parasite in grouper farming, than the longtooth grou...
Source: Developmental and Comparative Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Mai Sakakibara Ryuichiro Aoki Shukei Masuma Osamu Nakamura Sho Shirakashi Shigeyuki Tsutsui Source Type: research

Molecular characterization and antimicrobial activity of cecropin family in Hermetia illucens
In this study, we analyzed the localization and gene structure of 33 cecropin molecules in the H. illucens genome and evaluated their activity against common human pathogens. The results showed that 32 cecropin molecules were concentrated on 1 chromosome, most with 2 exons. More importantly, most of the cecropins had a good antibacterial effect against Gram-negative bacteria, and were not hemolytic. The minimum inhibitory concentration (MIC) of the cecropin designated H3 against E. coli was 4 μg/mL. The toxicity, killing time kinetics, and anti-biofilm activity of H3 were further investigated and confirmed its antimicrobi...
Source: Developmental and Comparative Immunology - December 11, 2023 Category: Allergy & Immunology Authors: Jian Peng Lu Li Yan Wan Yifan Yang Xiaoqin An Kexin Yuan Zhilang Qiu Yinhui Jiang Guo Guo Feng Shen Guiyou Liang Source Type: research

Evolutionarily conserved IL-22 participates in gut mucosal barrier through its receptors IL-22BP, IL-10R2 and IL-22RA1 during bacterial infection in teleost
Dev Comp Immunol. 2023 Dec 9:105110. doi: 10.1016/j.dci.2023.105110. Online ahead of print.ABSTRACTIL-22 is a critical cytokine of epithelial mucosal barrier. In humans, IL-22 signals through a heteroduplex receptor consisting of IL-22R and IL-10Rβ. In fish, IL-22 and its receptors homologues have been cloned in a number of species, however, no studies have been reported how the receptors are involved in IL-22 transduction. For this purpose, in this study we identified IL-22 and its soluble receptor IL-22BP and transmembrane receptors IL-22RA1 and IL-10R2 in Carassius cuvieri × Carassius auratus red var. (named WR-IL-22,...
Source: Developmental and Comparative Immunology - December 11, 2023 Category: Allergy & Immunology Authors: Qiongyao Zeng Xiaofeng Liu Yiyang Tang Zhengwei Li Ye Yang Niewen Hu Qingfeng Liu Zejun Zhou Source Type: research

Identification and structural analysis of dimeric chicken complement component 3d and its binding with chicken complement receptor 2
In this study, we aimed to explored whether chicken C3d (chC3d) exhibits similar characteristics, namely dimerization and binding of dimeric chC3d to chicken CR2 (chCR2). We investigated the interaction and co-localization of chC3d with itself using coimmunoprecipitation and confocal laser scanning microscopy, respectively. Then, dimeric chC3d was detected using native polyacrylamide gel electrophoresis and western blotting, and its equilibrium dissociation constant KD (827 nM) was determined using surface plasmon resonance. Finally, the interaction modes of dimeric chC3d were identified using molecular docking simulations...
Source: Developmental and Comparative Immunology - December 7, 2023 Category: Allergy & Immunology Authors: Huan Jin Min Tu Zhaoying Meng Bo Jiang Qianqian Yang Yongqing Li Zhenhua Zhang Source Type: research