Probing contacts of inhibitor locked in transition states in the catalytic triad of DENV2 type serine protease and its mutants by 1H, 19F and 15 N NMR spectroscopy
Detailed structural knowledge of enzyme-inhibitor complexes trapped in intermediate state is the key for a fundamental understanding of reaction mechanisms taking place in enzymes and is indispensable as a str... (Source: BMC Cell Biology)
Source: BMC Cell Biology - May 25, 2020 Category: Cytology Authors: Peter Agback, Esmeralda Woestenenk and Tatiana Agback Tags: Research article Source Type: research
Identification of the molecular mechanism and diagnostic biomarkers in the thoracic ossification of the ligamentum flavum using metabolomics and transcriptomics
To establish a metabolite fingerprint of ossification of the thoracic ligamentum flavum (OTLF) patients using liquid chromatography-mass spectrometry (LC-MS) in combination with transcriptomic data and explore... (Source: BMC Cell Biology)
Source: BMC Cell Biology - May 13, 2020 Category: Cytology Authors: Jiahao Li, Lingjia Yu, Shigong Guo and Yu Zhao Tags: Research article Source Type: research
Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome
The SMC5/6 complex, cohesin and condensin are the three mammalian members of the structural maintenance of chromosomes (SMC) family, large ring-like protein complexes that are essential for genome maintenance.... (Source: BMC Cell Biology)
Source: BMC Cell Biology - May 8, 2020 Category: Cytology Authors: Andr ás Horváth, Gergely Rona, Michele Pagano and Philip W. Jordan Tags: Research article Source Type: research
Bacterial cell growth is arrested by violet and blue, but not yellow light excitation during fluorescence microscopy
Fluorescence microscopy is a powerful tool in cell biology, especially for the study of dynamic processes. Intensive irradiation of bacteria with UV, blue and violet light has been shown to be able to kill cel... (Source: BMC Cell Biology)
Source: BMC Cell Biology - May 1, 2020 Category: Cytology Authors: Nina El Najjar, Muriel C. F. van Teeseling, Benjamin Mayer, Silke Hermann, Martin Thanbichler and Peter L. Graumann Tags: Research article Source Type: research
Basic biochemical characterization of cytosolic enzymes in thymidine nucleotide synthesis in adult rat tissues: implications for tissue specific mitochondrial DNA depletion and deoxynucleoside-based therapy for TK2-deficiency
Deficiency in thymidine kinase 2 (TK2) or p53 inducible ribonucleotide reductase small subunit (p53R2) is associated with tissue specific mitochondrial DNA (mtDNA) depletion. To understand the mechanisms of th... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 28, 2020 Category: Cytology Authors: Liya Wang, Ren Sun and Staffan Eriksson Tags: Research article Source Type: research
Retinoic acid receptor-related orphan receptor α reduces lipid droplets by upregulating neutral cholesterol ester hydrolase 1 in macrophages
Neutral cholesterol ester hydrolase 1 (NCEH1) catalyzes the hydrolysis of cholesterol ester (CE) in macrophages. Genetic ablation of NCEH1 promotes CE-laden macrophages and the development of atherosclerosis i... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 22, 2020 Category: Cytology Authors: Hiroshi Matsuoka, Riki Tokunaga, Miyu Katayama, Yuichiro Hosoda, Kaoruko Miya, Kento Sumi, Ami Ohishi, Jun Kamishikiryo, Akiho Shima and Akihiro Michihara Tags: Research article Source Type: research
Diazoxide affects mitochondrial bioenergetics by the opening of mKATP channel on submicromolar scale
Cytoprotection afforded by mitochondrial ATP-sensitive K+-channel (mKATP-channel) opener diazoxide (DZ) largely depends on the activation of potassium cycle with eventual modulation of mitochondrial functions and... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 19, 2020 Category: Cytology Authors: Olga Akopova, Liudmila Kolchinskaya, Valentina Nosar, Iryna Mankovska and Vadim Sagach Tags: Research article Source Type: research
The mitochondrial outer membrane protein SYNJ2BP interacts with the cell adhesion molecule TMIGD1 and can recruit it to mitochondria
Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) is a recently identified cell adhesion molecule which is predominantly expressed by epithelial cells of the intestine and the kidney. Its e... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 17, 2020 Category: Cytology Authors: Christian Hartmann, Ysabel Alessa Schwietzer, Daniel Kummer, Nils Kirschnick, Esther Hoppe, Eva-Maria Th üring, Mark Glaesner-Ebnet, Frauke Brinkmann, Volker Gerke, Stefan Reuter, Masanori Nakayama and Klaus Ebnet Tags: Research article Source Type: research
EphB4/ TNFR2/ERK/MAPK signaling pathway comprises a signaling axis to mediate the positive effect of TNF- α on osteogenic differentiation
Low concentrations of tumor necrosis factor-alpha (TNF- α) and its receptor TNFR2 are both reported to promote osteogenic differentiation of osteoblast precursor cells. Moreover, low concentrations of TNF-α up-... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 16, 2020 Category: Cytology Authors: Yu Zhang, Chengzhe Yang, Shaohua Ge, Limei Wang, Jin Zhang and Pishan Yang Tags: Research article Source Type: research
The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts
Reversible cell cycle arrest (quiescence/G0) is characteristic of adult stem cells and is actively controlled at multiple levels. Quiescent cells also extend a primary cilium, which functions as a signaling hu... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 15, 2020 Category: Cytology Authors: Nisha Venugopal, Ananga Ghosh, Hardik Gala, Ajoy Aloysius, Neha Vyas and Jyotsna Dhawan Tags: Research article Source Type: research
PGRMC1 effects on metabolism, genomic mutation and CpG methylation imply crucial roles in animal biology and disease
Progesterone receptor membrane component 1 (PGRMC1) is often elevated in cancers, and exists in alternative states of phosphorylation. A motif centered on PGRMC1 Y180 was evolutionarily acquired concurrently w... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 15, 2020 Category: Cytology Authors: Bashar M. Thejer, Partho P. Adhikary, Sarah L. Teakel, Johnny Fang, Paul A. Weston, Saliya Gurusinghe, Ayad G. Anwer, Martin Gosnell, Jalal A. Jazayeri, Marina Ludescher, Lesley-Ann Gray, Michael Pawlak, Robyn H. Wallace, Sameer D. Pant, Marie Wong, Tamas Tags: Research article Source Type: research
CHD9 upregulates RUNX2 and has a potential role in skeletal evolution
Changes in gene regulation are widely recognized as an important driver of adaptive phenotypic evolution. However, the specific molecular mechanisms that underpin such changes are still poorly understood. Chro... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 15, 2020 Category: Cytology Authors: Axel H. Newton and Andrew J. Pask Tags: Research article Source Type: research
Analyses of the folding sites of irregular β-trefoil fold proteins through sequence-based techniques and Gō-model simulations
The details of the folding mechanisms have not yet been fully understood for many proteins, and it is believed that the information on the folding mechanism of a protein is encoded in its amino acid sequence. ... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 15, 2020 Category: Cytology Authors: Risako Kimura, Panyavut Aumpuchin, Shoya Hamaue, Takumi Shimomura and Takeshi Kikuchi Tags: Research article Source Type: research
PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth
Progesterone Receptor Membrane Component 1 (PGRMC1) is expressed in many cancer cells, where it is associated with detrimental patient outcomes. It contains phosphorylated tyrosines which evolutionarily preced... (Source: BMC Cell Biology)
Source: BMC Cell Biology - April 3, 2020 Category: Cytology Authors: Bashar M. Thejer, Partho P. Adhikary, Amandeep Kaur, Sarah L. Teakel, Ashleigh Van Oosterum, Ishith Seth, Marina Pajic, Katherine M. Hannan, Megan Pavy, Perlita Poh, Jalal A. Jazayeri, Thiri Zaw, Dana Pascovici, Marina Ludescher, Michael Pawlak, Juan C. C Tags: Research article Source Type: research
Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades
Ubiquitination and ubiquitin-like protein post-translational modifications play an enormous number of roles in cellular processes. These modifications are constituted of multistep reaction cascades. Readily im... (Source: BMC Cell Biology)
Source: BMC Cell Biology - March 30, 2020 Category: Cytology Authors: Gabriel Fenteany, Paras Gaur, Gaurav Sharma, Lajos Pint ér, Ernő Kiss and Lajos Haracska Tags: Methodology article Source Type: research
