Caveolin-2 palmitoylation turnover facilitates insulin receptor substrate-1-directed lipid metabolism by insulin receptor tyrosine kinase
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 15:167173. doi: 10.1016/j.bbadis.2024.167173. Online ahead of print.ABSTRACTHere, we show that insulin induces palmitoylation turnover of Caveolin-2 (Cav-2) in adipocytes. Acyl protein thioesterases-1 (APT1) catalyzes Cav-2 depalmitoylation, and zinc finger DHHC domain-containing protein palmitoyltransferase 21 (ZDHHC21) repalmitoylation of the depalmitoylated Cav-2 for the turnover, thereby controlling insulin receptor (IR)-Cav-2-insulin receptor substrate-1 (IRS-1)-Akt-driven signaling. Insulin-induced palmitoylation turnover of Cav-2 facilitated glucose uptake and fat storage...
Source: Biochimica et Biophysica Acta - April 17, 2024 Category: Biochemistry Authors: Moonjeong Choi Jaewoong Lee Kyuho Jeong Yunbae Pak Source Type: research
High phosphate and calcium induce osteoblastic phenotype switching and calcification of corneal epithelial cells in a Runx2-dependent and synergistic manner; a possible mechanism of chronic kidney disease-associated corneal calcification
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 15:167171. doi: 10.1016/j.bbadis.2024.167171. Online ahead of print.ABSTRACTPatients with advanced chronic kidney disease (CKD) have elevated circulating calcium × phosphate product levels and exhibit soft tissue calcification. Besides the cardiovascular system, calcification is commonly observed in the cornea in CKD patients on hemodialysis. Cardiovascular calcification is a cell-mediated, highly regulated process, and we hypothesized that a similar regulatory mechanism is implicated in corneal calcification with the involvement of corneal epithelial cells (CECs). We establish...
Source: Biochimica et Biophysica Acta - April 17, 2024 Category: Biochemistry Authors: Haneen Ababneh Andrea T óth Gr éta Lente Enik ő Balogh D ávid Máté Csiki B éla Nagy Árpád Szöőr Vikt ória Jeney Source Type: research
Fe/S proteins in microbial sulfur oxidation
This article is part of a Special Issue entitled: Biogenesis and Function of Fe/S proteins.PMID:38631440 | DOI:10.1016/j.bbamcr.2024.119732 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 17, 2024 Category: Biochemistry Authors: Carolin K ümpel Martina Grosser Tomohisa Sebastian Tanabe Christiane Dahl Source Type: research
When iron and sulfur met on an anoxic planet and eventually made clusters essential for life
Biochim Biophys Acta Mol Cell Res. 2024 Apr 15:119730. doi: 10.1016/j.bbamcr.2024.119730. Online ahead of print.ABSTRACT[FeS] clusters are co-factors that are essential for life and are synthesized by dedicated multiprotein cellular machineries. In this review, we present the current scenario for the emergence and the diversification of the [FeS] cluster biosynthesis machineries. In addition to well-known NIF, ISC and SUF machineries, two alternative minimal systems, SMS, and MIS, were recently identified. Taxonomic distribution and phylogeny analyses indicate that SMS and MIS were present in the Last Universal Common Ance...
Source: Biochimica et Biophysica Acta - April 17, 2024 Category: Biochemistry Authors: Pierre Simon Garcia Simonetta Gribaldo Fr édéric Barras Source Type: research
Shared functions of FeS cluster assembly and Moco biosynthesis
Biochim Biophys Acta Mol Cell Res. 2024 Apr 15:119731. doi: 10.1016/j.bbamcr.2024.119731. Online ahead of print.ABSTRACTMolybdenum cofactor (Moco) biosynthesis is a complex process that involves the coordinated function of several proteins. In the recent years it has become evident that the availability of FeS clusters play an important role for the biosynthesis of Moco. First, the MoaA protein binds two [4Fe-4S] clusters per monomer. Second, the expression of the moaABCDE and moeAB operons is regulated by FNR, which senses the availability of oxygen via a functional [4Fe4S] cluster. Finally, the conversion of cyclic pyran...
Source: Biochimica et Biophysica Acta - April 17, 2024 Category: Biochemistry Authors: Muhammad Abrar Hasnat Silke Leimk ühler Source Type: research
Similar metabolic pathways are affected in both Congenital Myasthenic Syndrome-22 and Prader-Willi Syndrome
In conclusion, we provide the first molecular parallels between CMS22 and PWS, raising the possibility that PREPL substrates might become therapeutic targets for treating both disorders.PMID:38626828 | DOI:10.1016/j.bbadis.2024.167175 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 16, 2024 Category: Biochemistry Authors: Kritika Bhalla Karen Rosier Yenthe Monnens Sandra Meulemans Ellen Vervoort Lieven Thorrez Patrizia Agostinis Daniel T Meier Anne Rochtus James L Resnick John W M Creemers Source Type: research
Neuronal control of microglia through the mitochondria
This study provides evidence that neurons may trigger microglia activation and subsequent neuroinflammation. In addition, we demonstrate that microglial activation may have a protective role in ameliorating neuronal innate immune activation, at least in the initial phase. This work challenges the current understanding of neuroinflammation by assigning the primary role to neurons.PMID:38626829 | DOI:10.1016/j.bbadis.2024.167167 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 16, 2024 Category: Biochemistry Authors: A R Pereira-Santos Emanuel Candeias J D Magalh ães Nuno Empadinhas A Raquel Esteves Sandra M Cardoso Source Type: research
Resveratrol alleviates acute lung injury in mice by promoting Pink1/Parkin-related mitophagy and inhibiting NLRP3 inflammasome activation
In conclusion, resveratrol ameliorated ALI in mice, potentially by inhibiting the activation of NLRP3 inflammasomes, activating the Pink1/Parkin pathway, and promoting mitophagy.PMID:38626830 | DOI:10.1016/j.bbagen.2024.130612 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 16, 2024 Category: Biochemistry Authors: Dongdong Wu Hui Zhang Fang Li Shuai Liu Yang Wang Zhao Zhang Jiannan Wang Qiuge Wu Source Type: research
Similar metabolic pathways are affected in both Congenital Myasthenic Syndrome-22 and Prader-Willi Syndrome
In conclusion, we provide the first molecular parallels between CMS22 and PWS, raising the possibility that PREPL substrates might become therapeutic targets for treating both disorders.PMID:38626828 | DOI:10.1016/j.bbadis.2024.167175 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 16, 2024 Category: Biochemistry Authors: Kritika Bhalla Karen Rosier Yenthe Monnens Sandra Meulemans Ellen Vervoort Lieven Thorrez Patrizia Agostinis Daniel T Meier Anne Rochtus James L Resnick John W M Creemers Source Type: research
Neuronal control of microglia through the mitochondria
This study provides evidence that neurons may trigger microglia activation and subsequent neuroinflammation. In addition, we demonstrate that microglial activation may have a protective role in ameliorating neuronal innate immune activation, at least in the initial phase. This work challenges the current understanding of neuroinflammation by assigning the primary role to neurons.PMID:38626829 | DOI:10.1016/j.bbadis.2024.167167 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 16, 2024 Category: Biochemistry Authors: A R Pereira-Santos Emanuel Candeias J D Magalh ães Nuno Empadinhas A Raquel Esteves Sandra M Cardoso Source Type: research
Resveratrol alleviates acute lung injury in mice by promoting Pink1/Parkin-related mitophagy and inhibiting NLRP3 inflammasome activation
In conclusion, resveratrol ameliorated ALI in mice, potentially by inhibiting the activation of NLRP3 inflammasomes, activating the Pink1/Parkin pathway, and promoting mitophagy.PMID:38626830 | DOI:10.1016/j.bbagen.2024.130612 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 16, 2024 Category: Biochemistry Authors: Dongdong Wu Hui Zhang Fang Li Shuai Liu Yang Wang Zhao Zhang Jiannan Wang Qiuge Wu Source Type: research
Photobleaching FRET-FLIM-ICS for quaternary structure quantification on cells. Theory and simulations
Biochim Biophys Acta Gen Subj. 2024 Apr 15;1868(6):130618. doi: 10.1016/j.bbagen.2024.130618. Online ahead of print.ABSTRACTThe oligomerization of proteins is an important biological control mechanism and has several functions in activity and stability of enzymes, structural proteins, ion channels and transcription factors. The determination of the relevant oligomeric states in terms of geometry (spatial extent), oligomer size (monomer or dimer or oligomer) and affinity (amounts of monomer, dimer and oligomer) is a challenging biophysical problem. Förster resonance energy transfer and fluorescence fluctuation spectroscopy...
Source: Biochimica et Biophysica Acta - April 15, 2024 Category: Biochemistry Authors: Andrew H A Clayton Source Type: research
Transcriptomic analysis of HEK293A cells with a CRISPR/Cas9-mediated TDP1 knockout
Biochim Biophys Acta Gen Subj. 2024 Apr 13:130616. doi: 10.1016/j.bbagen.2024.130616. Online ahead of print.ABSTRACTTyrosyl-DNA phosphodiesterase 1 (TDP1) is a human DNA repair protein. It is a member of the phospholipase D family based on structural similarity. TDP1 is a key enzyme of the repair of stalled topoisomerase 1 (TOP1)-DNA complexes. Previously, with the CRISPR/Cas9 method, we obtained HEK293A cells with a homozygous knockout of the TDP1 gene and used the TDP1 knockout cells as a cellular model for studying mechanisms of action of an anticancer therapy. In the present work, we hypothesized that the TDP1 knockout...
Source: Biochimica et Biophysica Acta - April 15, 2024 Category: Biochemistry Authors: Nadezhda S Dyrkheeva Alexandra L Zakharenko Anastasia A Malakhova Larisa S Okorokova Dmitry N Shtokalo Sergey P Medvedev Alexey A Tupikin Marsel R Kabilov Olga I Lavrik Source Type: research
Photobleaching FRET-FLIM-ICS for quaternary structure quantification on cells. Theory and simulations
Biochim Biophys Acta Gen Subj. 2024 Apr 13:130618. doi: 10.1016/j.bbagen.2024.130618. Online ahead of print.ABSTRACTThe oligomerization of proteins is an important biological control mechanism and has several functions in activity and stability of enzymes, structural proteins, ion channels and transcription factors. The determination of the relevant oligomeric states in terms of geometry (spatial extent), oligomer size (monomer or dimer or oligomer) and affinity (amounts of monomer, dimer and oligomer) is a challenging biophysical problem. Förster resonance energy transfer and fluorescence fluctuation spectroscopy are pow...
Source: Biochimica et Biophysica Acta - April 15, 2024 Category: Biochemistry Authors: Andrew H A Clayton Source Type: research
Transcriptomic analysis of HEK293A cells with a CRISPR/Cas9-mediated TDP1 knockout
Biochim Biophys Acta Gen Subj. 2024 Apr 13:130616. doi: 10.1016/j.bbagen.2024.130616. Online ahead of print.ABSTRACTTyrosyl-DNA phosphodiesterase 1 (TDP1) is a human DNA repair protein. It is a member of the phospholipase D family based on structural similarity. TDP1 is a key enzyme of the repair of stalled topoisomerase 1 (TOP1)-DNA complexes. Previously, with the CRISPR/Cas9 method, we obtained HEK293A cells with a homozygous knockout of the TDP1 gene and used the TDP1 knockout cells as a cellular model for studying mechanisms of action of an anticancer therapy. In the present work, we hypothesized that the TDP1 knockout...
Source: Biochimica et Biophysica Acta - April 15, 2024 Category: Biochemistry Authors: Nadezhda S Dyrkheeva Alexandra L Zakharenko Anastasia A Malakhova Larisa S Okorokova Dmitry N Shtokalo Sergey P Medvedev Alexey A Tupikin Marsel R Kabilov Olga I Lavrik Source Type: research
