Therapeutic targeting of telomerase ameliorates experimental choroidal neovascularization
In conclusion, our study identifies telomerase as a promising therapeutic target for treating neovascular disease of the eye and thus provides a proof of principle for further exploration of telomerase inhibition as a novel treatment strategy for nvAMD.PMID:38582267 | DOI:10.1016/j.bbadis.2024.167156 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: Aman Kumar Yosuke Nagasaka Vinodhini Jayananthan Asmaa Zidan Tyler Heisler-Taylor Jayakrishna Ambati Shigeo Tamiya Nagaraj Kerur Source Type: research
Cellular functions, molecular signalings and therapeutic applications: Translational potential of deubiquitylating enzyme USP9X as a drug target in cancer treatment
Biochim Biophys Acta Rev Cancer. 2024 Apr 4;1879(3):189099. doi: 10.1016/j.bbcan.2024.189099. Online ahead of print.ABSTRACTProtein ubiquitination, one of the most significant post-translational modifications, plays an important role in controlling the proteins activity in diverse cellular processes. The reversible process of protein ubiquitination, known as deubiquitination, has emerged as a critical mechanism for maintaining cellular homeostasis. The deubiquitinases (DUBs), which participate in deubiquitination process are increasingly recognized as potential candidates for drug discovery. Among these DUBs, ubiquitin-spe...
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: Hongli Gao Zhiguang Chen Liang Zhao Ce Ji Fei Xing Source Type: research
A role of methionines in the functioning of oxidatively modified fibrinogen
Biochim Biophys Acta Proteins Proteom. 2024 Apr 4:141013. doi: 10.1016/j.bbapap.2024.141013. Online ahead of print.ABSTRACTPosttranslational modifications in fibrinogen resulting from induced oxidation or oxidative stress in the organism can have deleterious influence on optimal functioning of fibrinogen, causing a disturbance in assembly and properties of fibrin. The protective mechanism supporting the ability of fibrinogen to function in ROS-generating environment remains completely unexplored. The effects of very low and moderately low HOCl/-OCl concentrations on fibrinogen oxidative modifications, the fibrin network st...
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: L V Yurina A D Vasilyeva E S Gavrilina V S Ivanov S I Obydennyi I A Chabin M I Indeykina A S Kononikhin E N Nikolaev M A Rosenfeld Source Type: research
Gut microbiota regulates the ALK5/NOX1 axis by altering glutamine metabolism to inhibit ferroptosis of intrahepatic cholangiocarcinoma cells
In this study, feces and plasma from healthy controls and ICC patients were collected for 16S rRNA sequencing or metabolomics analysis. Gut microbiota analysis showed that gut microbiota abundance and biodiversity were altered in ICC patients compared with controls. Plasma metabolism analysis showed that the metabolite glutamine content of the ICC patient was significantly higher than that of the controls. KEGG pathway analysis showed that glutamine plays a vital role in ICC. In addition, the use of antibiotics in ICC animals further confirmed that changes in gut microbiota affect changes in glutamine. Further experiments ...
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: Qi Zhang Jixiang Zhou Denggao Zhai Qin Jiang Manyi Yang Maojun Zhou Source Type: research
Network analysis of S-nitrosylated synaptic proteins demonstrates unique roles in health and disease
Biochim Biophys Acta Mol Cell Res. 2024 Apr 4:119720. doi: 10.1016/j.bbamcr.2024.119720. Online ahead of print.ABSTRACTNitric oxide can covalently modify cysteine thiols on target proteins to alter that protein's function in a process called S-nitrosylation (SNO). S-nitrosylation of synaptic proteins plays an integral part in neurotransmission. Here we review the function of the SNO-proteome at the synapse and whether clusters of SNO-modification may predict synaptic dysfunction associated with disease. We used a systematic search strategy to concatenate SNO-proteomic datasets from normal human or murine brain samples. Ide...
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: Morgan G Stykel Scott D Ryan Source Type: research
Idebenone ameliorates statin-induced myotoxicity in atherosclerotic ApoE-/- mice by reducing oxidative stress and improving mitochondrial function
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 4:167157. doi: 10.1016/j.bbadis.2024.167157. Online ahead of print.ABSTRACTStatins are the first line of choice for the treatment for atherosclerosis, but their use can cause myotoxicity, a common side effect that may require dosage reduction or discontinuation. The exact mechanism of statin-induced myotoxicity is unknown. Previous research has demonstrated that the combination of idebenone and statin yielded superior anti-atherosclerotic outcomes. Here, we investigated the mechanism of statin-induced myotoxicity in atherosclerotic ApoE-/- mice and whether idebenone could counte...
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: Wenfei Yu Wenjing Wu Dandan Zhao Rui ZhangMD Kai Shao Haoyang Liu Chuanzhu Yan Pengfei Lin Source Type: research
Therapeutic targeting of telomerase ameliorates experimental choroidal neovascularization
In conclusion, our study identifies telomerase as a promising therapeutic target for treating neovascular disease of the eye and thus provides a proof of principle for further exploration of telomerase inhibition as a novel treatment strategy for nvAMD.PMID:38582267 | DOI:10.1016/j.bbadis.2024.167156 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: Aman Kumar Yosuke Nagasaka Vinodhini Jayananthan Asmaa Zidan Tyler Heisler-Taylor Jayakrishna Ambati Shigeo Tamiya Nagaraj Kerur Source Type: research
Cellular functions, molecular signalings and therapeutic applications: Translational potential of deubiquitylating enzyme USP9X as a drug target in cancer treatment
Biochim Biophys Acta Rev Cancer. 2024 Apr 4:189099. doi: 10.1016/j.bbcan.2024.189099. Online ahead of print.ABSTRACTProtein ubiquitination, one of the most significant post-translational modifications, plays an important role in controlling the proteins activity in diverse cellular processes. The reversible process of protein ubiquitination, known as deubiquitination, has emerged as a critical mechanism for maintaining cellular homeostasis. The deubiquitinases (DUBs), which participate in deubiquitination process are increasingly recognized as potential candidates for drug discovery. Among these DUBs, ubiquitin-specific pr...
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: Hongli Gao Zhiguang Chen Liang Zhao Ce Ji Fei Xing Source Type: research
A role of methionines in the functioning of oxidatively modified fibrinogen
Biochim Biophys Acta Proteins Proteom. 2024 Apr 4:141013. doi: 10.1016/j.bbapap.2024.141013. Online ahead of print.ABSTRACTPosttranslational modifications in fibrinogen resulting from induced oxidation or oxidative stress in the organism can have deleterious influence on optimal functioning of fibrinogen, causing a disturbance in assembly and properties of fibrin. The protective mechanism supporting the ability of fibrinogen to function in ROS-generating environment remains completely unexplored. The effects of very low and moderately low HOCl/-OCl concentrations on fibrinogen oxidative modifications, the fibrin network st...
Source: Biochimica et Biophysica Acta - April 6, 2024 Category: Biochemistry Authors: L V Yurina A D Vasilyeva E S Gavrilina V S Ivanov S I Obydennyi I A Chabin M I Indeykina A S Kononikhin E N Nikolaev M A Rosenfeld Source Type: research
Toll like receptor 4 mediates the inhibitory effect of SARS-CoV-2 spike protein on proximal tubule albumin endocytosis
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 3:167155. doi: 10.1016/j.bbadis.2024.167155. Online ahead of print.ABSTRACTTubular proteinuria is a common feature in COVID-19 patients, even in the absence of established acute kidney injury. SARS-CoV-2 spike protein (S protein) was shown to inhibit megalin-mediated albumin endocytosis in proximal tubule epithelial cells (PTECs). Angiotensin-converting enzyme type 2 (ACE2) was not directly involved. Since Toll-like receptor 4 (TLR4) mediates S protein effects in various cell types, we hypothesized that TLR4 could be participating in the inhibition of PTECs albumin endocytosis e...
Source: Biochimica et Biophysica Acta - April 5, 2024 Category: Biochemistry Authors: Rodrigo P Silva-Aguiar Douglas E Teixeira Diogo B Peruchetti Rodrigo A S Peres Sarah A S Alves Pedro T Calil Luciana B Arruda Luciana J Costa Pedro L Silva Alvin H Schmaier Patricia R M Rocco Ana Acacia S Pinheiro Celso Caruso-Neves Source Type: research
Differential effects of theasinensins and epigallocatechin-3-O-gallate on phospholipid bilayer structure and liposomal aggregation
This study presents a differential analysis of intact and oxidized EGCg regarding their interactions with phosphatidylcholine liposomes, serving as a simple biomembrane model. In the presence of ascorbic acid, pre-oxidized EGCg induced liposomal aggregation in a dose-dependent manner, whereas intact EGCg did not. Toxicity evaluation using calcein-loaded liposomes revealed that liposomal aggregation is associated with minimal membrane damage. Through fractionation of the oxidized EGCg sample, the fraction containing theasinensins showed high liposomal aggregation activity. Overall, these results suggest that oxidatively con...
Source: Biochimica et Biophysica Acta - April 5, 2024 Category: Biochemistry Authors: Asako Narai-Kanayama Sumio Hayakawa Takayuki Yoshino Futa Honda Hiroko Matsuda Yumiko Oishi Source Type: research
E2F transcription factor-1 modulates expression of glutamine metabolic genes in mouse embryonic fibroblasts and uterine sarcoma cells
Biochim Biophys Acta Mol Cell Res. 2024 Apr 3:119721. doi: 10.1016/j.bbamcr.2024.119721. Online ahead of print.ABSTRACTMetabolic reprogramming is considered as a hallmark of cancer and is clinically exploited as a novel target for therapy. The E2F transcription factor-1 (E2F1) regulates various cellular processes, including proliferative and metabolic pathways, and acts, depending on the cellular and molecular context, as an oncogene or tumor suppressor. The latter is evident by the observation that E2f1-knockout mice develop spontaneous tumors, including uterine sarcomas. This dual role warrants a detailed investigation o...
Source: Biochimica et Biophysica Acta - April 5, 2024 Category: Biochemistry Authors: Katharina Huber Albert Giralt Ren é Dreos Helene Michenthaler Sarah Geller Valentin Barquissau Dorian V Ziegler Daniele Tavernari Hector Gallart-Ayala Katarina Krajina Katharina Jonas Giovanni Ciriello Julijana Ivanisevic Andreas Prokesch Martin Pichler Source Type: research
Toll like receptor 4 mediates the inhibitory effect of SARS-CoV-2 spike protein on proximal tubule albumin endocytosis
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 3:167155. doi: 10.1016/j.bbadis.2024.167155. Online ahead of print.ABSTRACTTubular proteinuria is a common feature in COVID-19 patients, even in the absence of established acute kidney injury. SARS-CoV-2 spike protein (S protein) was shown to inhibit megalin-mediated albumin endocytosis in proximal tubule epithelial cells (PTECs). Angiotensin-converting enzyme type 2 (ACE2) was not directly involved. Since Toll-like receptor 4 (TLR4) mediates S protein effects in various cell types, we hypothesized that TLR4 could be participating in the inhibition of PTECs albumin endocytosis e...
Source: Biochimica et Biophysica Acta - April 5, 2024 Category: Biochemistry Authors: Rodrigo P Silva-Aguiar Douglas E Teixeira Diogo B Peruchetti Rodrigo A S Peres Sarah A S Alves Pedro T Calil Luciana B Arruda Luciana J Costa Pedro L Silva Alvin H Schmaier Patricia R M Rocco Ana Acacia S Pinheiro Celso Caruso-Neves Source Type: research
Differential effects of theasinensins and epigallocatechin-3-O-gallate on phospholipid bilayer structure and liposomal aggregation
This study presents a differential analysis of intact and oxidized EGCg regarding their interactions with phosphatidylcholine liposomes, serving as a simple biomembrane model. In the presence of ascorbic acid, pre-oxidized EGCg induced liposomal aggregation in a dose-dependent manner, whereas intact EGCg did not. Toxicity evaluation using calcein-loaded liposomes revealed that liposomal aggregation is associated with minimal membrane damage. Through fractionation of the oxidized EGCg sample, the fraction containing theasinensins showed high liposomal aggregation activity. Overall, these results suggest that oxidatively con...
Source: Biochimica et Biophysica Acta - April 5, 2024 Category: Biochemistry Authors: Asako Narai-Kanayama Sumio Hayakawa Takayuki Yoshino Futa Honda Hiroko Matsuda Yumiko Oishi Source Type: research
E2F transcription factor-1 modulates expression of glutamine metabolic genes in mouse embryonic fibroblasts and uterine sarcoma cells
Biochim Biophys Acta Mol Cell Res. 2024 Apr 3:119721. doi: 10.1016/j.bbamcr.2024.119721. Online ahead of print.ABSTRACTMetabolic reprogramming is considered as a hallmark of cancer and is clinically exploited as a novel target for therapy. The E2F transcription factor-1 (E2F1) regulates various cellular processes, including proliferative and metabolic pathways, and acts, depending on the cellular and molecular context, as an oncogene or tumor suppressor. The latter is evident by the observation that E2f1-knockout mice develop spontaneous tumors, including uterine sarcomas. This dual role warrants a detailed investigation o...
Source: Biochimica et Biophysica Acta - April 5, 2024 Category: Biochemistry Authors: Katharina Huber Albert Giralt Ren é Dreos Helene Michenthaler Sarah Geller Valentin Barquissau Dorian V Ziegler Daniele Tavernari Hector Gallart-Ayala Katarina Krajina Katharina Jonas Giovanni Ciriello Julijana Ivanisevic Andreas Prokesch Martin Pichler Source Type: research
