Chaperone function in Fe-S protein biogenesis: Three possible scenarios
Biochim Biophys Acta Mol Cell Res. 2024 Apr 2:119717. doi: 10.1016/j.bbamcr.2024.119717. Online ahead of print.ABSTRACTAmong the six known iron‑sulfur (FeS) cluster biogenesis machineries that function across all domains of life only one involves a molecular chaperone system. This machinery, called ISC for 'iron sulfur cluster', functions in bacteria and in mitochondria of eukaryotes including humans. The chaperone system - a dedicated J-domain protein co-chaperone termed Hsc20 and its Hsp70 partner - is essential for proper ISC machinery function, interacting with the scaffold protein IscU which serves as a platform for...
Source: Biochimica et Biophysica Acta - April 4, 2024 Category: Biochemistry Authors: Jaroslaw Marszalek Elizabeth A Craig Marcin Pitek Rafal Dutkiewicz Source Type: research
Rise of palmitoylation: A new trick to tune NCX1 activity
Biochim Biophys Acta Mol Cell Res. 2024 Apr 2:119719. doi: 10.1016/j.bbamcr.2024.119719. Online ahead of print.ABSTRACTThe cardiac Na+/Ca2+ Exchanger (NCX1) controls transmembrane calcium flux in numerous tissues. The only reversible post-translational modification established to regulate NCX1 is palmitoylation, which alters the ability of the exchanger to inactivate. Palmitoylation creates a binding site for the endogenous XIP domain, a region of the NCX1 intracellular loop established to inactivate NCX1. The binding site created by NCX1 palmitoylation sensitizes the transporter to XIP. Herein we summarize our recent know...
Source: Biochimica et Biophysica Acta - April 4, 2024 Category: Biochemistry Authors: Caglar G ök William Fuller Source Type: research
Harnessing iron ‑sulfur enzymes for synthetic biology
Biochim Biophys Acta Mol Cell Res. 2024 Apr 2:119718. doi: 10.1016/j.bbamcr.2024.119718. Online ahead of print.ABSTRACTReactions catalysed by iron‑sulfur (FeS) enzymes appear in a variety of biosynthetic pathways that produce valuable natural products. Harnessing these biosynthetic pathways by expression in microbial cell factories grown on an industrial scale would yield enormous economic and environmental benefits. However, FeS enzymes often become bottlenecks that limits the productivity of engineered pathways. As a consequence, achieving the production metrics required for industrial application remains a distant goa...
Source: Biochimica et Biophysica Acta - April 4, 2024 Category: Biochemistry Authors: Helena Shomar Gregory Bokinsky Source Type: research
Regulation of oxidative stress in the intestine of piglets after enterotoxigenic Escherichia coli (ETEC) infection
Biochim Biophys Acta Mol Cell Res. 2024 Apr 2:119711. doi: 10.1016/j.bbamcr.2024.119711. Online ahead of print.ABSTRACTEnterotoxigenic Escherichia coli (ETEC) is recognized globally as a major gastrointestinal pathogen that impairs intestinal function. ETEC infection can lead to oxidative stress and disruption of intestinal integrity. The present study investigated the mechanism of increased oxidative stress and whether restoration of antioxidant defense could improve intestinal integrity in a piglet model with ETEC infection. Weaned piglets were divided into three groups: control, ETEC-infection and ETEC-infection with an...
Source: Biochimica et Biophysica Acta - April 4, 2024 Category: Biochemistry Authors: Shunshun Jin Haoxiang Xu Chengbo Yang O Karmin Source Type: research
TG-interacting factor 1 regulates mitotic clonal expansion during adipocyte differentiation
In conclusion, we strengthen the pivotal role of TGIF1 in early differentiation, which might contribute to resolving obesity-associated metabolic syndromes.PMID:38575107 | DOI:10.1016/j.bbalip.2024.159492 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 4, 2024 Category: Biochemistry Authors: Yu-Hao Chang Yu-Hua Tseng Ju-Ming Wang Yau-Sheng Tsai Huei-Sheng Huang Source Type: research
Modification of the RTX domain cap by acyl chains of adapted length rules the formation of functional hemolysin pores
Biochim Biophys Acta Biomembr. 2024 Apr 1:184311. doi: 10.1016/j.bbamem.2024.184311. Online ahead of print.ABSTRACTThe acylated pore-forming Repeats in ToXin (RTX) cytolysins α-hemolysin (HlyA) and adenylate cyclase toxin (CyaA) preferentially bind to β2 integrins of myeloid leukocytes but can also promiscuously bind and permeabilize cells lacking the β2 integrins. We constructed a HlyA1-563/CyaA860-1706 chimera that was acylated either by the toxin-activating acyltransferase CyaC, using sixteen carbon-long (C16) acyls, or by the HlyC acyltransferase using fourteen carbon-long (C14) acyls. Cytolysin assays with the C16-...
Source: Biochimica et Biophysica Acta - April 3, 2024 Category: Biochemistry Authors: Anna Lepesheva Michaela Grobarcikova Adriana Osickova David Jurnecka Sarka Knoblochova Monika Cizkova Radim Osicka Peter Sebo Jiri Masin Source Type: research
Modification of the RTX domain cap by acyl chains of adapted length rules the formation of functional hemolysin pores
Biochim Biophys Acta Biomembr. 2024 Apr 1:184311. doi: 10.1016/j.bbamem.2024.184311. Online ahead of print.ABSTRACTThe acylated pore-forming Repeats in ToXin (RTX) cytolysins α-hemolysin (HlyA) and adenylate cyclase toxin (CyaA) preferentially bind to β2 integrins of myeloid leukocytes but can also promiscuously bind and permeabilize cells lacking the β2 integrins. We constructed a HlyA1-563/CyaA860-1706 chimera that was acylated either by the toxin-activating acyltransferase CyaC, using sixteen carbon-long (C16) acyls, or by the HlyC acyltransferase using fourteen carbon-long (C14) acyls. Cytolysin assays with the C16-...
Source: Biochimica et Biophysica Acta - April 3, 2024 Category: Biochemistry Authors: Anna Lepesheva Michaela Grobarcikova Adriana Osickova David Jurnecka Sarka Knoblochova Monika Cizkova Radim Osicka Peter Sebo Jiri Masin Source Type: research
Tracing the lipidome in inborn errors of metabolism
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Mar 31:159491. doi: 10.1016/j.bbalip.2024.159491. Online ahead of print.ABSTRACTInborn errors of metabolism (IEM) represent a heterogeneous group of more than 1800 rare disorders, many of which are causing significant childhood morbidity and mortality. More than 100 IEM are linked to dyslipidaemia, but yet our knowledge in connecting genetic information with lipidomic data is limited. Stable isotope tracing studies of the lipid metabolism (STL) provide insights on the dynamic of cellular lipid processes and could thereby facilitate the delineation of underlying metabolic (pat...
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Martina Zandl-Lang Source Type: research
Tumor vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA): Suppresses macrophage capping protein beyond STING activation
In this study, we demonstrated that DMXAA binds to both human and mouse macrophage capping protein (CapG), with a KD of 5.839 μM for hCapG and a KD of 2.867 μM for mCapG, as determined by surface plasmon resonance (SPR) analysis. Homology modeling and molecular docking analysis of hCapG indicated that the critical residues involved in the hydrogen bond interaction of DMXAA with hCapG were Arg153, Thr151, and GLN141, Asn234. In addition, electrostatic pi-cation interaction occurred between DMXAA and hCapG. Further functional studies revealed that CapG protein plays a crucial role in the effects of DMXAA on human umbilical...
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Zhiyong Xiao Xia Cui Feng Liu Ying Wang Xiao Liu Wenxia Zhou Yongxiang Zhang Source Type: research
Rabeprazole mitigates obesity-induced chronic inflammation and insulin resistance associated with increased M2-type macrophage polarization
Biochim Biophys Acta Mol Basis Dis. 2024 Mar 31:167142. doi: 10.1016/j.bbadis.2024.167142. Online ahead of print.ABSTRACTMacrophage polarization is closely associated with obesity-induced chronic inflammation and insulin resistance. Proton pump inhibitor Rabeprazole has long been used to treat gastritis and gastric ulcers. However, whether Rabeprazole plays a role in macrophage polarization during obesity is unknown. Here, we show that Rabeprazole suppresses M1-type macrophage-mediated inflammation, leads to increased M2-type macrophages and alters the polarization status from M1 to M2 in vitro. Mechanistically, Rabe-regul...
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Yunfang Li Jiayue Hao Xianghui Kong Weiyi Yuan Yingying Shen Zhaoyuan Hui Xinliang Lu Source Type: research
PTPRO inhibition ameliorates spinal cord injury through shifting microglial M1/M2 polarization via the NF- κB/STAT6 signaling pathway
In conclusion, PTPRO inhibition improves spinal cord injury through facilitating M2 microglia polarization via the NF-κB/STAT6 signaling pathway, which is probably controlled by SOX4.PMID:38565385 | DOI:10.1016/j.bbadis.2024.167141 (Source: Biochimica et Biophysica Acta)
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Haocong Zhang Liangbi Xiang Hong Yuan Hailong Yu Source Type: research
OTUD1 enhances gastric cancer aggressiveness by deubiquitinating EBV-encoded protein BALF1 to stabilize the apoptosis inhibitor Bcl-2
This study focuses on EBV-encoded BALF1 (BamH1 A fragment leftward reading frame 1), a key apoptosis regulator in EBV-related cancers, whose specific impact on EBVaGC was previously unknown. Our findings indicate that BALF1 overexpression in gastric cancer cells significantly enhances their proliferation, migration, and resistance to chemotherapy-induced apoptosis, confirming BALF1's oncogenic potential. A novel discovery is that BALF1 undergoes degradation via the ubiquitin-proteasome pathway. Through analysis of 69 deubiquitinating enzymes (DUBs), ovarian tumor protease (OTU) domain-containing protein 1 (OTUD1) emerged a...
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Hanbin Lin Yuting Han Yuchao Sang Yuecheng Wu Mengyue Tian Xintan Chen Xu Lin Xinjian Lin Source Type: research
Extracellular CIRP co-stimulated T cells through IL6R/STAT3 in pediatric IgA vasculitis
Biochim Biophys Acta Mol Basis Dis. 2024 Apr 1:167151. doi: 10.1016/j.bbadis.2024.167151. Online ahead of print.ABSTRACTImmunoglobulin A vasculitis (IgAV) is the most common vasculitis of childhood. Disordered immune responses play important roles in its pathogenesis, but the comprehensive immune profile of the disease and the underlying mechanisms are still largely unknown. Here we found a potential disease biomarker cold inducible RNA binding protein (CIRP) in our pediatric IgAV cohort. Serum CIRP level in these patients were elevated and positively correlated with the increased early memory (CD45RA+CD62L+CD95+) T cells ...
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Hong Lei Dan Li Mingyue Duan Yuanyuan Zhang Ying Sun Jun She Xi Zhang Petra Reinke Hans-Dieter Volk Yanmin Zhang Yi Lv Rongqian Wu Source Type: research
Tracing the lipidome in inborn errors of metabolism
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Mar 31:159491. doi: 10.1016/j.bbalip.2024.159491. Online ahead of print.ABSTRACTInborn errors of metabolism (IEM) represent a heterogeneous group of more than 1800 rare disorders, many of which are causing significant childhood morbidity and mortality. More than 100 IEM are linked to dyslipidaemia, but yet our knowledge in connecting genetic information with lipidomic data is limited. Stable isotope tracing studies of the lipid metabolism (STL) provide insights on the dynamic of cellular lipid processes and could thereby facilitate the delineation of underlying metabolic (pat...
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Martina Zandl-Lang Source Type: research
Tumor vascular disrupting agent 5,6-dimethylxanthenone-4-acetic acid (DMXAA): Suppresses macrophage capping protein beyond STING activation
In this study, we demonstrated that DMXAA binds to both human and mouse macrophage capping protein (CapG), with a KD of 5.839 μM for hCapG and a KD of 2.867 μM for mCapG, as determined by surface plasmon resonance (SPR) analysis. Homology modeling and molecular docking analysis of hCapG indicated that the critical residues involved in the hydrogen bond interaction of DMXAA with hCapG were Arg153, Thr151, and GLN141, Asn234. In addition, electrostatic pi-cation interaction occurred between DMXAA and hCapG. Further functional studies revealed that CapG protein plays a crucial role in the effects of DMXAA on human umbilical...
Source: Biochimica et Biophysica Acta - April 2, 2024 Category: Biochemistry Authors: Zhiyong Xiao Xia Cui Feng Liu Ying Wang Xiao Liu Wenxia Zhou Yongxiang Zhang Source Type: research
