Immuno-informatic prediction of B and T cell epitopes of cysteine protease allergen from Phaseolus vulgaris with cross-reactive potential and population coverage
CONCLUSION: The current approach can be applied for identification of epitopes. Analysis of cross-reactive and widely-distributed specific epitopes of allergen and knowledge about their interactive surfaces will help in understanding of food allergy and related immune responses.PMID:35927799 | DOI:10.2174/1389203723666220804124927 (Source: Current Protein and Peptide Science)
Source: Current Protein and Peptide Science - August 5, 2022 Category: Biochemistry Authors: Akansha Sharma Srishti Vashisht Shailendra Nath Gaur Janendra K Batra Naveen Arora Source Type: research
Immuno-informatic prediction of B and T cell epitopes of cysteine protease allergen from Phaseolus vulgaris with cross-reactive potential and population coverage
CONCLUSION: The current approach can be applied for identification of epitopes. Analysis of cross-reactive and widely-distributed specific epitopes of allergen and knowledge about their interactive surfaces will help in understanding of food allergy and related immune responses.PMID:35927799 | DOI:10.2174/1389203723666220804124927 (Source: Current Protein and Peptide Science)
Source: Current Protein and Peptide Science - August 5, 2022 Category: Biochemistry Authors: Akansha Sharma Srishti Vashisht Shailendra Nath Gaur Janendra K Batra Naveen Arora Source Type: research
Exposure to iprodione induces ROS production and mitochondrial dysfunction in porcine trophectoderm and uterine luminal epithelial cells, leading to implantation defects during early pregnancy
This study demonstrated a series of cytotoxic responses of iprodione along with the alteration of implantation-related gene expression in porcine trophectoderm (pTr) and luminal epithelium (pLE) cells. In this study, iprodione suppressed cell viability, proliferation, and migration of these cells. Iprodione induced G1 phase arrest and attenuated spheroid formation by pTr and pLE cells. Furthermore, iprodione caused mitochondrial dysfunction and excessive reactive oxygen species generation, which resulted in an increase in mitochondrial calcium levels. Consequently, DNA damage and apoptotic cell death were induced by iprodi...
Source: Chemosphere - August 4, 2022 Category: Chemistry Authors: Wonhyoung Park Garam An Whasun Lim Gwonhwa Song Source Type: research
Exposure to iprodione induces ROS production and mitochondrial dysfunction in porcine trophectoderm and uterine luminal epithelial cells, leading to implantation defects during early pregnancy
This study demonstrated a series of cytotoxic responses of iprodione along with the alteration of implantation-related gene expression in porcine trophectoderm (pTr) and luminal epithelium (pLE) cells. In this study, iprodione suppressed cell viability, proliferation, and migration of these cells. Iprodione induced G1 phase arrest and attenuated spheroid formation by pTr and pLE cells. Furthermore, iprodione caused mitochondrial dysfunction and excessive reactive oxygen species generation, which resulted in an increase in mitochondrial calcium levels. Consequently, DNA damage and apoptotic cell death were induced by iprodi...
Source: Chemosphere - August 4, 2022 Category: Chemistry Authors: Wonhyoung Park Garam An Whasun Lim Gwonhwa Song Source Type: research
Exposure to iprodione induces ROS production and mitochondrial dysfunction in porcine trophectoderm and uterine luminal epithelial cells, leading to implantation defects during early pregnancy
This study demonstrated a series of cytotoxic responses of iprodione along with the alteration of implantation-related gene expression in porcine trophectoderm (pTr) and luminal epithelium (pLE) cells. In this study, iprodione suppressed cell viability, proliferation, and migration of these cells. Iprodione induced G1 phase arrest and attenuated spheroid formation by pTr and pLE cells. Furthermore, iprodione caused mitochondrial dysfunction and excessive reactive oxygen species generation, which resulted in an increase in mitochondrial calcium levels. Consequently, DNA damage and apoptotic cell death were induced by iprodi...
Source: Chemosphere - August 4, 2022 Category: Chemistry Authors: Wonhyoung Park Garam An Whasun Lim Gwonhwa Song Source Type: research
Is the central mesencephalic reticular formation a purely horizontal gaze center?
Brain Struct Funct. 2022 Jul 24. doi: 10.1007/s00429-022-02532-8. Online ahead of print.ABSTRACTHistorically, the central mesencephalic reticular formation has been regarded as a purely horizontal gaze center based on the fact that electrical stimulation of this region produces horizontal saccades, it provides monosynaptic input to medial rectus motoneurons, and cells recorded in this region often display a peak in firing when horizontal saccades are made. We tested the proposition that the central mesencephalic reticular formation is purely a horizontal gaze center by examining whether this region also supplies terminals ...
Source: Brain Structure and Function - July 24, 2022 Category: Neuroscience Authors: Martin O Bohlen Susan Warren Paul J May Source Type: research
Is the central mesencephalic reticular formation a purely horizontal gaze center?
Brain Struct Funct. 2022 Jul 24. doi: 10.1007/s00429-022-02532-8. Online ahead of print.ABSTRACTHistorically, the central mesencephalic reticular formation has been regarded as a purely horizontal gaze center based on the fact that electrical stimulation of this region produces horizontal saccades, it provides monosynaptic input to medial rectus motoneurons, and cells recorded in this region often display a peak in firing when horizontal saccades are made. We tested the proposition that the central mesencephalic reticular formation is purely a horizontal gaze center by examining whether this region also supplies terminals ...
Source: Brain Structure and Function - July 24, 2022 Category: Neuroscience Authors: Martin O Bohlen Susan Warren Paul J May Source Type: research
Reuniting the Three Sisters: collaborative science with Native growers to improve soil and community health
AbstractBefore Euro-American settlement, many Native American nations intercropped maize (Zea mays), beans (Phaseolus vulgaris), and squash (Cucurbita pepo) in what is colloquially called the “Three Sisters.” Here we review the historic importance and consequences of rejuvenation of Three Sisters intercropping (3SI), outline a framework to engage Native growers in community science with positive feedbacks to university research, and present preliminary findings from ethnography and a randomized, replicated 3SI experiment. We developed mutually beneficial collaborative research agendas with four Midwestern US Native Ame...
Source: Agriculture and Human Values - July 17, 2022 Category: Food Science Source Type: research
Phytotoxic effect of the insecticide imidacloprid in Phaseolus vulgaris L. plant and evaluation of its bioaccumulation and translocation by electrochemical methods
The objective of this work is to study the toxicological effect of the imidacloprid (IMD) on common bean plants (Phaseolus vulgaris L) when used at high doses and its quantification by electrochemical method. Common bean plants were exposed to increasing concentrations of IMD and the different plant tissues were subjected to various analyses. The IMD detection in different tissues of the bean plant was performed after extraction on the metallic silver electrode using square wave voltammetry. The analytical and calibration parameters (Slope, correlation coefficient, linear range, detection limit and relative standard deviat...
Source: Environmental Research - July 9, 2022 Category: Environmental Health Authors: N Ajermoun S Aghris F Ettadili O Tahiri Alaoui F Laghrib A Farahi S Lahrich M Bakasse S Saqrane M A El Mhammedi Source Type: research
Phytotoxic effect of the insecticide imidacloprid in Phaseolus vulgaris L. plant and evaluation of its bioaccumulation and translocation by electrochemical methods
The objective of this work is to study the toxicological effect of the imidacloprid (IMD) on common bean plants (Phaseolus vulgaris L) when used at high doses and its quantification by electrochemical method. Common bean plants were exposed to increasing concentrations of IMD and the different plant tissues were subjected to various analyses. The IMD detection in different tissues of the bean plant was performed after extraction on the metallic silver electrode using square wave voltammetry. The analytical and calibration parameters (Slope, correlation coefficient, linear range, detection limit and relative standard deviat...
Source: Environmental Research - July 9, 2022 Category: Environmental Health Authors: N Ajermoun S Aghris F Ettadili O Tahiri Alaoui F Laghrib A Farahi S Lahrich M Bakasse S Saqrane M A El Mhammedi Source Type: research
Clue of zinc oxide and copper oxide nanoparticles in the remediation of cadmium toxicity in Phaseolus vulgaris L. via the modulation of antioxidant and redox systems
Environ Sci Pollut Res Int. 2022 Jul 6. doi: 10.1007/s11356-022-21799-2. Online ahead of print.ABSTRACTThe present study represents new evidence of the successful use of metal oxide nanoparticles in the remediation of heavy metals. Zinc oxide nanoparticles (ZnO NP) and copper oxide nanoparticles (CuO NP) were assessed to alleviate cadmium (Cd) toxicity in Phaseolus vulgaris L. seedlings and plants. Monitoring physiological and metabolic parameters allowed to elucidate Cd mechanism and process whereby it exerts phytotoxic effects on bean. The response of P. vulgaris seedlings is NP dose-dependent (10 mg/L, 50 mg/L, 100 mg/L...
Source: Environmental Science and Pollution Research International - July 6, 2022 Category: Environmental Health Authors: Safa Hidouri In ès Karmous Oumaima Kadri Oussama Kharbech Abdelilah Chaoui Source Type: research
A novel nepovirus causing a chlorotic fleck disease on common bean (Phaseolus vulgaris L.)
AbstractTwo common bean leaf samples from Ethiopia that had shown chlorotic fleck and veinal mosaic symptoms but tested ELISA-negative for known viruses were mechanically transmitted to herbaceous hosts to obtain virus isolates ET-773/4 and ET-779. Virus purification fromChenopodium quinoa systemically infected with ET-773/4 yielded icosahedral particles measuring ~ 30 nm in diameter and containing a single capsid protein of ~ 58 kDa, suggesting a nepovirus infection. Analysis of nucleotide sequences generated from RNA1 and RNA2 of the isolates indicated that they represent a distinct virus species in the genusNepov...
Source: Archives of Virology - July 6, 2022 Category: Virology Source Type: research
Biological contamination of the common bean (Phaseolus vulgaris L.) and its impact on food safety
(Source: Critical Reviews in Food Science and Nutrition)
Source: Critical Reviews in Food Science and Nutrition - July 3, 2022 Category: Nutrition Authors: Duarte Santos, Thaisa Badiale Furlong, Eliana Source Type: research
Biofortification of common bean (Phaseolus vulgaris L.) with iron and zinc: Achievements and challenges
This review describes the genes and processes associated with Fe and Zn accumulation in common bean, a significant food source in Africa that plays an important role in nutritional security. We discuss the conventional plant breeding, transgenic and gene editing approaches that are being deployed to improve Fe and Zn accumulation in beans. We also consider the requirements of successful bean biofortification programmes, highlighting gaps in current knowledge, possible solutions and future perspectives. AbstractMicronutrient deficiencies (hidden hunger), particularly in iron (Fe) and zinc (Zn), remain one of the most seriou...
Source: Food and Energy Security - June 30, 2022 Category: Food Science Authors: Raul Huertas,
Barbara Karpinska,
Sophia Ngala,
Bertha Mkandawire,
Joyce Maling'a,
Elizabeth Wajenkeche,
Paul M. Kimani,
Christine Boesch,
Derek Stewart,
Robert D. Hancock,
Christine H. Foyer Tags: REVIEW Source Type: research
