Viral metatranscriptomic approach to study the diversity of virus(es) associated with Common Bean (Phaseolus vulgaris L.) in the North-Western Himalayan region of India
In this study, we surveyed the common bean fields of Kashmir valley for virus infection using a total of 140 symptomatic and non-symptomatic leaf samples collected from different locations. The genetic diversity of viruses was examined by high-throughput sequencing (HTS) with three viruses being identified, namely, Bean Common Mosaic Virus (BCMV), Bean Common Mosaic Necrosis Virus (BCMNV), and Clover Yellow Vein Virus (ClYVV). BCMNV and ClYVV are new reports from India. De novo assembly of transcriptome constructed near-complete genomes of these viruses. RT-PCR results confirmed the presence of these viruses with an emerge...
Source: Frontiers in Microbiology - September 21, 2022 Category: Microbiology Source Type: research

Biomass-based indole derived composited with cotton cellulose fiber integrated as sensitive fluorescence platform for NH < sub > 3 < /sub > detection and monitoring of seafood spoilage
Int J Biol Macromol. 2022 Sep 14;221:994-1001. doi: 10.1016/j.ijbiomac.2022.09.095. Online ahead of print.ABSTRACTHerein, an indole-derived water-soluble fluorescence nanomaterial and biomass-based cellulose filter paper integrated as solid-state fluorescence platform (H2-FP) for seafood spoilage detection was prepared. H2 exhibits high fluorescence stability and good biocompatibility with green beans, onion tissues, blood and zebrafish, which proving that H2 has a wide range of application scenarios. Further, H2-FP with effective, solid-state fluorescence, portable, and reusable characteristics is nanoengineered for NH3 q...
Source: International Journal of Biological Macromolecules - September 16, 2022 Category: Biochemistry Authors: Yingying Zhang Long Chen Jianhua Huang Anquan Yang Jing Wang Min Xie Yuqian Liu Zhulan Liu Huining Xiao Huihua Min Chenyao Hu Ranhua Xiong Chaobo Huang Source Type: research

Crystallization and crystallographic studies of a novel chickpea 11S globulin
Chickpea is a crop that is known as a source of high-quality proteins. CL-AI, which belongs to the 11S globulin and cupin superfamily, was initially identified in chickpea seeds. CL-AI has recently been shown to inhibit various types of α -amylases. To determine its molecular mechanism, the crystal structure of CL-AI was solved at a final resolution of 2.2   Å . Structural analysis indicated that each asymmetric unit contains three molecules with threefold symmetry and a head-to-tail association, and each molecule is divided into an α -chain and a β -chain. CL-AI has high structural similarity to other 11S globulins ...
Source: Acta Crystallographica Section F - August 22, 2022 Category: Biochemistry Authors: Sun, L. Zhou, A. Zhang, F. Tags: 11S globulins chickpeas crystal structure cupin proteins CL-AI research communications Source Type: research

Sensors, Vol. 22, Pages 6106: Monitoring and Identification of Agricultural Crops through Multitemporal Analysis of Optical Images and Machine Learning Algorithms
-Ruíz The information about where crops are distributed is useful for agri-environmental assessments, but is chiefly important for food security and agricultural policy managers. The quickness with which this information becomes available, especially over large areas, is important for decision makers. Methodologies have been proposed for the study of crops. Most of them require field survey for ground truth data and a single crop map is generated for the whole season at the end of the crop cycle and for the next crop cycle a new field survey is necessary. Here, we present models for recognizing maize (Zea mays L.), be...
Source: Sensors - August 16, 2022 Category: Biotechnology Authors: Jos é M. Espinosa-Herrera Antonia Macedo-Cruz Demetrio S. Fern ández-Reynoso H éctor Flores-Magdaleno Yolanda M. Fern ández-Ordoñez Jes ús Soria-Ruíz Tags: Article Source Type: research

Phaseolus vulgaris extract ameliorates high-fat diet-induced colonic barrier dysfunction and inflammation in mice by regulating peroxisome proliferator-activated receptor expression and butyrate levels
Obesity is a health concern worldwide, and its onset is multifactorial. In addition to metabolic syndrome, a high-fat diet induces many deleterious downstream effects, such as chronic systemic inflammation, a loss of gut barrier integrity, and gut microbial dysbiosis, with a reduction of many butyrate-producing bacteria. These conditions can be ameliorated by increasing legumes in the daily diet. White and kidney beans (Phaseolus vulgaris L.) and their non-nutritive bioactive component phaseolamin were demonstrated to mitigate several pathological features related to a metabolic syndrome-like condition. The aim of the pres...
Source: Frontiers in Pharmacology - August 11, 2022 Category: Drugs & Pharmacology Source Type: research

Genes, Vol. 13, Pages 1410: Determining Genetic Diversity and Population Structure of Common Bean (Phaseolus vulgaris L.) Landraces from T & uuml;rkiye Using SSR Markers
This study aimed to determine the genetic diversity of 40 common bean (Phaseolus vulgaris L.) landraces collected from the Ispir district located in the Northeast Anatolia region of T&amp;uuml;rkiye and five commercial varieties using SSR markers. The Twenty-seven SSR markers produced a total of 142 polymorphic bands, ranging from 2 (GATS91 and PVTT001) to 12 (BM153) alleles per marker, with an average number of 5.26 alleles. The gene diversity per marker varied between 0.37 and 0.87 for BM053 and BM153 markers, respectively. When heterozygous individuals are calculated proportional to the population, the heterozygosit...
Source: Genes - August 8, 2022 Category: Genetics & Stem Cells Authors: G üller Özkan Kamil Halilo ğlu Aras T ürkoğlu Halil Ibrahim Özturk Erdal Elkoca Peter Poczai Tags: Article Source Type: research

Origin of thyrotropin-releasing hormone neurons that innervate the tuberomammillary nuclei
Brain Struct Funct. 2022 Aug 7. doi: 10.1007/s00429-022-02527-5. Online ahead of print.ABSTRACTHypophysiotropic thyrotropin-releasing hormone (TRH) neurons function as metabolic sensors that regulate the thyroid axis and energy homeostasis. Less is known about the role of other hypothalamic TRH neurons. As central administration of TRH decreases food intake and increases histamine in the tuberomammillary nuclei (TMN), and TMN histamine neurons are densely innervated by TRH fibers from an unknown origin, we mapped the location of TRH neurons that project to the TMN. The retrograde tracer, cholera toxin B subunit (CTB), was ...
Source: Brain Structure and Function - August 7, 2022 Category: Neuroscience Authors: Edith S ánchez-Jaramillo G ábor Wittmann Judit Menyh ért Praful Singru Gabriela B G ómez-González Eduardo S ánchez-Islas Nashiely Y áñez-Recendis Jaime Arturo Pimentel-Cabrera Martha Le ón-Olea Bal ázs Gereben Csaba Fekete Jean-Louis Charli Rona Source Type: research

Origin of thyrotropin-releasing hormone neurons that innervate the tuberomammillary nuclei
Brain Struct Funct. 2022 Aug 7. doi: 10.1007/s00429-022-02527-5. Online ahead of print.ABSTRACTHypophysiotropic thyrotropin-releasing hormone (TRH) neurons function as metabolic sensors that regulate the thyroid axis and energy homeostasis. Less is known about the role of other hypothalamic TRH neurons. As central administration of TRH decreases food intake and increases histamine in the tuberomammillary nuclei (TMN), and TMN histamine neurons are densely innervated by TRH fibers from an unknown origin, we mapped the location of TRH neurons that project to the TMN. The retrograde tracer, cholera toxin B subunit (CTB), was ...
Source: Brain Structure and Function - August 7, 2022 Category: Neuroscience Authors: Edith S ánchez-Jaramillo G ábor Wittmann Judit Menyh ért Praful Singru Gabriela B G ómez-González Eduardo S ánchez-Islas Nashiely Y áñez-Recendis Jaime Arturo Pimentel-Cabrera Martha Le ón-Olea Bal ázs Gereben Csaba Fekete Jean-Louis Charli Rona Source Type: research

Origin of thyrotropin-releasing hormone neurons that innervate the tuberomammillary nuclei
AbstractHypophysiotropic thyrotropin-releasing hormone (TRH) neurons function as metabolic sensors that regulate the thyroid axis and energy homeostasis. Less is known about the role of other hypothalamic TRH neurons. As central administration of TRH decreases food intake and increases histamine in the tuberomammillary nuclei (TMN), and TMN histamine neurons are densely innervated by TRH fibers from an unknown origin, we mapped the location of TRH neurons that project to the TMN. The retrograde tracer, cholera toxin B subunit (CTB), was injected into the TMN E1 –E2, E4–E5 subdivisions of adult Sprague–Dawley male rat...
Source: Anatomy and Embryology - August 7, 2022 Category: Anatomy Source Type: research

Immuno-informatic prediction of B and T cell epitopes of cysteine protease allergen from Phaseolus vulgaris with cross-reactive potential and population coverage
CONCLUSION: The current approach can be applied for identification of epitopes. Analysis of cross-reactive and widely-distributed specific epitopes of allergen and knowledge about their interactive surfaces will help in understanding of food allergy and related immune responses.PMID:35927799 | DOI:10.2174/1389203723666220804124927 (Source: Current Protein and Peptide Science)
Source: Current Protein and Peptide Science - August 5, 2022 Category: Biochemistry Authors: Akansha Sharma Srishti Vashisht Shailendra Nath Gaur Janendra K Batra Naveen Arora Source Type: research

Immuno-informatic prediction of B and T cell epitopes of cysteine protease allergen from Phaseolus vulgaris with cross-reactive potential and population coverage
CONCLUSION: The current approach can be applied for identification of epitopes. Analysis of cross-reactive and widely-distributed specific epitopes of allergen and knowledge about their interactive surfaces will help in understanding of food allergy and related immune responses.PMID:35927799 | DOI:10.2174/1389203723666220804124927 (Source: Current Protein and Peptide Science)
Source: Current Protein and Peptide Science - August 5, 2022 Category: Biochemistry Authors: Akansha Sharma Srishti Vashisht Shailendra Nath Gaur Janendra K Batra Naveen Arora Source Type: research

Immuno-informatic prediction of B and T cell epitopes of cysteine protease allergen from Phaseolus vulgaris with cross-reactive potential and population coverage
CONCLUSION: The current approach can be applied for identification of epitopes. Analysis of cross-reactive and widely-distributed specific epitopes of allergen and knowledge about their interactive surfaces will help in understanding of food allergy and related immune responses.PMID:35927799 | DOI:10.2174/1389203723666220804124927 (Source: Current Protein and Peptide Science)
Source: Current Protein and Peptide Science - August 5, 2022 Category: Biochemistry Authors: Akansha Sharma Srishti Vashisht Shailendra Nath Gaur Janendra K Batra Naveen Arora Source Type: research

Immuno-informatic prediction of B and T cell epitopes of cysteine protease allergen from Phaseolus vulgaris with cross-reactive potential and population coverage
CONCLUSION: The current approach can be applied for identification of epitopes. Analysis of cross-reactive and widely-distributed specific epitopes of allergen and knowledge about their interactive surfaces will help in understanding of food allergy and related immune responses.PMID:35927799 | DOI:10.2174/1389203723666220804124927 (Source: Current Protein and Peptide Science)
Source: Current Protein and Peptide Science - August 5, 2022 Category: Biochemistry Authors: Akansha Sharma Srishti Vashisht Shailendra Nath Gaur Janendra K Batra Naveen Arora Source Type: research