Evaluation of an in vitro experimental platform of human polarized intestinal epithelial monolayers for the hazard assessment of insecticidal proteins
Food Chem Toxicol. 2023 Oct 16:114106. doi: 10.1016/j.fct.2023.114106. Online ahead of print.ABSTRACTPrevious work demonstrated the utility of using human-derived intestinal epithelial cell (IEC) lines cultured as polarized monolayers on Transwell® filters to differentiate between hazardous and non-hazardous proteins. The current study seeks to further resolve appropriate concentrations for evaluating proteins of unknown hazard potential using the IEC experimental platform and leverages these parameters for evaluating the potential toxicity of insecticidal proteins characteristic of those expressed in genetically modified...
Source: Food and Chemical Toxicology - October 18, 2023 Category: Food Science Authors: Cindi S Zimmermann Ryan F Snow Brittany Wilson-Mifsud Kristine LeRoy Chad Boeckman Emily Huang Carey A Mathesius Jason M Roper Bryan P Hurley Source Type: research
Evaluation of an in vitro experimental platform of human polarized intestinal epithelial monolayers for the hazard assessment of insecticidal proteins
Food Chem Toxicol. 2023 Oct 16:114106. doi: 10.1016/j.fct.2023.114106. Online ahead of print.ABSTRACTPrevious work demonstrated the utility of using human-derived intestinal epithelial cell (IEC) lines cultured as polarized monolayers on Transwell® filters to differentiate between hazardous and non-hazardous proteins. The current study seeks to further resolve appropriate concentrations for evaluating proteins of unknown hazard potential using the IEC experimental platform and leverages these parameters for evaluating the potential toxicity of insecticidal proteins characteristic of those expressed in genetically modified...
Source: Food and Chemical Toxicology - October 18, 2023 Category: Food Science Authors: Cindi S Zimmermann Ryan F Snow Brittany Wilson-Mifsud Kristine LeRoy Chad Boeckman Emily Huang Carey A Mathesius Jason M Roper Bryan P Hurley Source Type: research
Impact of a retrograded starch ingredient obtained from negro Jamapa beans (Phaseolus vulgaris L. Negro Jamapa) on glucose metabolism and oxidative stress in induced diabetic lab rats model
Int J Biol Macromol. 2023 Oct 14:127447. doi: 10.1016/j.ijbiomac.2023.127447. Online ahead of print.ABSTRACTA retrograded starch ingredient obtained from Negro Jampa beans (Phaseolus vulgaris L. Negro Jamapa), applying a debranching process (18 U/g for 12 h) and retrogradation (2 °C for 3 days), was evaluated as a potential functional ingredient to improve glucose homeostasis in a diabetic animal model. The obtained ingredient was reduced in rapid digestible starch amount compared to its related isolated native starch (34.1 % and 53.6 %, respectively) and resistant (33.4 % and 22.3 %, respectively). Therefore, a reduced t...
Source: International Journal of Biological Macromolecules - October 16, 2023 Category: Biochemistry Authors: Teodoro Su árez Diéguez Mariza Olvera N ájera Marcos Galv án Juan Antonio Nieto Source Type: research
Impact of a retrograded starch ingredient obtained from negro Jamapa beans (Phaseolus vulgaris L. Negro Jamapa) on glucose metabolism and oxidative stress in induced diabetic lab rats model
Int J Biol Macromol. 2023 Oct 14:127447. doi: 10.1016/j.ijbiomac.2023.127447. Online ahead of print.ABSTRACTA retrograded starch ingredient obtained from Negro Jampa beans (Phaseolus vulgaris L. Negro Jamapa), applying a debranching process (18 U/g for 12 h) and retrogradation (2 °C for 3 days), was evaluated as a potential functional ingredient to improve glucose homeostasis in a diabetic animal model. The obtained ingredient was reduced in rapid digestible starch amount compared to its related isolated native starch (34.1 % and 53.6 %, respectively) and resistant (33.4 % and 22.3 %, respectively). Therefore, a reduced t...
Source: International Journal of Biological Macromolecules - October 16, 2023 Category: Biochemistry Authors: Teodoro Su árez Diéguez Mariza Olvera N ájera Marcos Galv án Juan Antonio Nieto Source Type: research
Identification of quantitative trait loci for drought tolerance in Bukoba/Kijivu Andean mapping population of common bean
The objective of this study was to identify the Quantitative Trait Loci (QTL) for drought tolerance in an Andean population of Recombinant Inbred Lines (RILs). A total of 155 F5:7 RILs derived from a cross between Kijivu (drought tolerant) and Bukoba (drought susceptible) were evaluated for drought tolerance in field and pot experiments. Four field experiments were conducted at three locations in Zambia in 2020 and 2021. All field trials were conducted in the dry season under irrigation. The 155 RILs were genotyped with 11,292 SNPs, and composite interval mapping was conducted to identify QTL for drought tolerance. Seed yi...
Source: TAG. Theoretical and Applied Genetics - October 12, 2023 Category: Genetics & Stem Cells Authors: Swivia M Hamabwe Nicholas Amimo Otieno Alvaro Soler-Garz ón Phillip N Miklas Travis Parker David M Kramer Abhijnan Chattopadhyay Pride Cheelo Kuwabo Kuwabo Kelvin Kamfwa Source Type: research
Identification of quantitative trait loci for drought tolerance in Bukoba/Kijivu Andean mapping population of common bean
The objective of this study was to identify the Quantitative Trait Loci (QTL) for drought tolerance in an Andean population of Recombinant Inbred Lines (RILs). A total of 155 F5:7 RILs derived from a cross between Kijivu (drought tolerant) and Bukoba (drought susceptible) were evaluated for drought tolerance in field and pot experiments. Four field experiments were conducted at three locations in Zambia in 2020 and 2021. All field trials were conducted in the dry season under irrigation. The 155 RILs were genotyped with 11,292 SNPs, and composite interval mapping was conducted to identify QTL for drought tolerance. Seed yi...
Source: TAG. Theoretical and Applied Genetics - October 12, 2023 Category: Genetics & Stem Cells Authors: Swivia M Hamabwe Nicholas Amimo Otieno Alvaro Soler-Garz ón Phillip N Miklas Travis Parker David M Kramer Abhijnan Chattopadhyay Pride Cheelo Kuwabo Kuwabo Kelvin Kamfwa Source Type: research
Identification of quantitative trait loci for drought tolerance in Bukoba/Kijivu Andean mapping population of common bean
The objective of this study was to identify the Quantitative Trait Loci (QTL) for drought tolerance in an Andean population of Recombinant Inbred Lines (RILs). A total of 155 F5:7 RILs derived from a cross between Kijivu (drought tolerant) and Bukoba (drought susceptible) were evaluated for drought tolerance in field and pot experiments. Four field experiments were conducted at three locations in Zambia in 2020 and 2021. All field trials were conducted in the dry season under irrigation. The 155 RILs were genotyped with 11,292 SNPs, and composite interval mapping was conducted to identify QTL for drought tolerance. Seed yi...
Source: TAG. Theoretical and Applied Genetics - October 12, 2023 Category: Genetics & Stem Cells Authors: Swivia M Hamabwe Nicholas Amimo Otieno Alvaro Soler-Garz ón Phillip N Miklas Travis Parker David M Kramer Abhijnan Chattopadhyay Pride Cheelo Kuwabo Kuwabo Kelvin Kamfwa Source Type: research
Molecules, Vol. 28, Pages 7030: Effect of Germinated Sorghum Extract on the Physical and Thermal Properties of Pre-Gelatinized Cereals, Sweet Potato and Beans Starches
This study investigated the impact of α-amylase on several properties of pre-gelatinized starches derived from chickpea (Cicer arietinum L.), wheat (Triticum aestivum L.), corn (Zea mays L.), white beans (Phaseolus vulgaris), and sweet potatoes (Ipomoea batatas L.). Specifically, the water holding capacity, freezable water content, sugar content, and water sorption isotherm (adsorption and desorption) properties were examined. The source of α-amylase utilized in this study was a germinated sorghum (Sorghum bicolor L. Moench) extract (GSE). The starch samples were subjected to annealing at temper...
Source: Molecules - October 11, 2023 Category: Chemistry Authors: Hesham Alqah Shahzad Hussain Mohamed Saleh Alamri Abdellatif A. Mohamed Akram A. Qasem Mohamed A. Ibraheem Aamir Shehzad Tags: Article Source Type: research
Automating high-throughput screening for anthracnose resistance in common bean using allele specific PCR
Common beans (Phaseolus vulgaris L.) provide important protein and calories globally. Anthracnose (Colletotrichum lindemuthianum (Sacc.& Magnus) Briosi& Cavara, 1889) is a major disease in common bean and cause... (Source: Plant Methods)
Source: Plant Methods - October 3, 2023 Category: Biology Authors: Marysia Zaleski-Cox, Phillip N. Miklas, Alvaro Soler-Garz ón and Valerio Hoyos-Villegas Tags: Methodology Source Type: research
Association of a novel begomovirus species with fenugreek yellow vein disease in India
CONCLUSIONS: In conclusion, the presence of a novel begomovirus species associated with yellow vein disease in fenugreek is alarming and needs further studies on its infectivity to prevent its spread to legume crops.PMID:37776416 | DOI:10.1007/s11033-023-08806-6 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - September 30, 2023 Category: Molecular Biology Authors: Manish Kumar Amalendu Ghosh Kuldeep Singh Jadon Baljeet Kaur Rajesh K Kakani Ramesh K Solanki Source Type: research
Association of a novel begomovirus species with fenugreek yellow vein disease in India
CONCLUSIONS: In conclusion, the presence of a novel begomovirus species associated with yellow vein disease in fenugreek is alarming and needs further studies on its infectivity to prevent its spread to legume crops.PMID:37776416 | DOI:10.1007/s11033-023-08806-6 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - September 30, 2023 Category: Molecular Biology Authors: Manish Kumar Amalendu Ghosh Kuldeep Singh Jadon Baljeet Kaur Rajesh K Kakani Ramesh K Solanki Source Type: research
Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris
Plant viruses of the genus Alphaendornavirus are transmitted solely via seed and pollen and generally cause no apparent disease. It has been conjectured that certain plant endornaviruses may confer advantages on ... (Source: Virology Journal)
Source: Virology Journal - September 22, 2023 Category: Virology Authors: Thomas J. Brine, Satish B. Viswanathan, Alex M. Murphy, Adrienne E. Pate, Francis O. Wamonje and John P. Carr Tags: Research Source Type: research
Chlorogenic acid in green bean coffee on body weight: a systematic review and meta-analysis of randomized controlled trials
ConclusionsAccording to the meta-analysis, GBCE with CGA 500 mg/day lowers body weight. Nevertheless, despite its limited sample size and short-term study, this study was successful. Long-term research on the effectiveness and safety of GBCE and CGA on body weight require more clinical trials.Systematic review registrationPROSPERO CRD42021254916. (Source: Systematic Reviews)
Source: Systematic Reviews - September 14, 2023 Category: International Medicine & Public Health Source Type: research
Effect of organic acid-soaking and sonication on the formation of volatile compounds and α-dicarbonyl compounds in Robusta coffee
This study suggested the pretreatment methods to enhance the flavor substances and reduce the α-DCs in Robusta coffee.PMID:37673014 | DOI:10.1016/j.ultsonch.2023.106580 (Source: Ultrasonics Sonochemistry)
Source: Ultrasonics Sonochemistry - September 6, 2023 Category: Chemistry Authors: Haeun Lee Jonggab Yim Youngji Lee Kwang-Geun Lee Source Type: research
