Berberine Protects Against Dihydrotestosterone-Induced Human Ovarian Granulosa Cell Injury and Ferroptosis by Regulating the Circ_0097636/MiR-186-5p/SIRT3 Pathway
This study aims to analyze the effect of BBR on a cell model of PCOS and the underlying mechanism. Human ovarian granulosa (KGN) cells were treated with dihydrotestosterone (DHT) to mimic a PCOS cell model. The RNA expression of circ_0097636, miR-186-5p, and sirtuin3 (SIRT3) was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was detected by western blotting. Cell viability was analyzed by CCK-8 assay. Cell proliferation and apoptosis were investigated by 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry assay, respectively. The levels of interleukin-6 (IL-6), IL-1β, and...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Suqin Wang Yingfang Wang Qin Qin Jianfang Li Qiaoyun Chen Ye Zhang Xiuqing Li Jianrong Liu Source Type: research
Berberine Protects Against Dihydrotestosterone-Induced Human Ovarian Granulosa Cell Injury and Ferroptosis by Regulating the Circ_0097636/MiR-186-5p/SIRT3 Pathway
This study aims to analyze the effect of BBR on a cell model of PCOS and the underlying mechanism. Human ovarian granulosa (KGN) cells were treated with dihydrotestosterone (DHT) to mimic a PCOS cell model. The RNA expression of circ_0097636, miR-186-5p, and sirtuin3 (SIRT3) was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was detected by western blotting. Cell viability was analyzed by CCK-8 assay. Cell proliferation and apoptosis were investigated by 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry assay, respectively. The levels of interleukin-6 (IL-6), IL-1β, and...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Suqin Wang Yingfang Wang Qin Qin Jianfang Li Qiaoyun Chen Ye Zhang Xiuqing Li Jianrong Liu Source Type: research
Berberine Protects Against Dihydrotestosterone-Induced Human Ovarian Granulosa Cell Injury and Ferroptosis by Regulating the Circ_0097636/MiR-186-5p/SIRT3 Pathway
This study aims to analyze the effect of BBR on a cell model of PCOS and the underlying mechanism. Human ovarian granulosa (KGN) cells were treated with dihydrotestosterone (DHT) to mimic a PCOS cell model. The RNA expression of circ_0097636, miR-186-5p, and sirtuin3 (SIRT3) was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was detected by western blotting. Cell viability was analyzed by CCK-8 assay. Cell proliferation and apoptosis were investigated by 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry assay, respectively. The levels of interleukin-6 (IL-6), IL-1β, and...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Suqin Wang Yingfang Wang Qin Qin Jianfang Li Qiaoyun Chen Ye Zhang Xiuqing Li Jianrong Liu Source Type: research
Berberine Protects Against Dihydrotestosterone-Induced Human Ovarian Granulosa Cell Injury and Ferroptosis by Regulating the Circ_0097636/MiR-186-5p/SIRT3 Pathway
This study aims to analyze the effect of BBR on a cell model of PCOS and the underlying mechanism. Human ovarian granulosa (KGN) cells were treated with dihydrotestosterone (DHT) to mimic a PCOS cell model. The RNA expression of circ_0097636, miR-186-5p, and sirtuin3 (SIRT3) was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was detected by western blotting. Cell viability was analyzed by CCK-8 assay. Cell proliferation and apoptosis were investigated by 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry assay, respectively. The levels of interleukin-6 (IL-6), IL-1β, and...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Suqin Wang Yingfang Wang Qin Qin Jianfang Li Qiaoyun Chen Ye Zhang Xiuqing Li Jianrong Liu Source Type: research
Metabolic characteristics of granulosa cell tumor: role of PPAR γ signaling
Biol Reprod. 2023 Dec 20:ioad173. doi: 10.1093/biolre/ioad173. Online ahead of print.ABSTRACTThe relative rarity of ovarian granulosa cell tumor (GCT) cases and preclinical models for GCT has posed a challenge for understanding GCT and developing therapeutics. Metabolic reprogramming is one of the key disruptions related to cancer progression. GCT exhibits significant lipid accumulation and overexpression of peroxisome proliferator-activated receptor gamma (PPARγ), a regulator of lipid metabolism. However, their roles remain unclear. Previously, we reported spontaneous GCT development in female mice with a constitutively ...
Source: Biology of Reproduction - December 20, 2023 Category: Reproduction Medicine Authors: Seok-Yeong Yu Yi Luan Pauline C Xu Yaqi Zhang Rosemary Dong Amirhossein Abazarikia So-Youn Kim Source Type: research
Metabolic characteristics of granulosa cell tumor: role of PPAR γ signaling
Biol Reprod. 2023 Dec 20:ioad173. doi: 10.1093/biolre/ioad173. Online ahead of print.ABSTRACTThe relative rarity of ovarian granulosa cell tumor (GCT) cases and preclinical models for GCT has posed a challenge for understanding GCT and developing therapeutics. Metabolic reprogramming is one of the key disruptions related to cancer progression. GCT exhibits significant lipid accumulation and overexpression of peroxisome proliferator-activated receptor gamma (PPARγ), a regulator of lipid metabolism. However, their roles remain unclear. Previously, we reported spontaneous GCT development in female mice with a constitutively ...
Source: Reproductive Biology - December 20, 2023 Category: Reproduction Medicine Authors: Seok-Yeong Yu Yi Luan Pauline C Xu Yaqi Zhang Rosemary Dong Amirhossein Abazarikia So-Youn Kim Source Type: research
Metabolic characteristics of granulosa cell tumor: role of PPAR γ signaling
Biol Reprod. 2023 Dec 20:ioad173. doi: 10.1093/biolre/ioad173. Online ahead of print.ABSTRACTThe relative rarity of ovarian granulosa cell tumor (GCT) cases and preclinical models for GCT has posed a challenge for understanding GCT and developing therapeutics. Metabolic reprogramming is one of the key disruptions related to cancer progression. GCT exhibits significant lipid accumulation and overexpression of peroxisome proliferator-activated receptor gamma (PPARγ), a regulator of lipid metabolism. However, their roles remain unclear. Previously, we reported spontaneous GCT development in female mice with a constitutively ...
Source: Biology of Reproduction - December 20, 2023 Category: Reproduction Medicine Authors: Seok-Yeong Yu Yi Luan Pauline C Xu Yaqi Zhang Rosemary Dong Amirhossein Abazarikia So-Youn Kim Source Type: research
Metabolic characteristics of granulosa cell tumor: role of PPAR γ signaling
Biol Reprod. 2023 Dec 20:ioad173. doi: 10.1093/biolre/ioad173. Online ahead of print.ABSTRACTThe relative rarity of ovarian granulosa cell tumor (GCT) cases and preclinical models for GCT has posed a challenge for understanding GCT and developing therapeutics. Metabolic reprogramming is one of the key disruptions related to cancer progression. GCT exhibits significant lipid accumulation and overexpression of peroxisome proliferator-activated receptor gamma (PPARγ), a regulator of lipid metabolism. However, their roles remain unclear. Previously, we reported spontaneous GCT development in female mice with a constitutively ...
Source: Reproductive Biology - December 20, 2023 Category: Reproduction Medicine Authors: Seok-Yeong Yu Yi Luan Pauline C Xu Yaqi Zhang Rosemary Dong Amirhossein Abazarikia So-Youn Kim Source Type: research
Metabolic characteristics of granulosa cell tumor: role of PPAR γ signaling
Biol Reprod. 2023 Dec 20:ioad173. doi: 10.1093/biolre/ioad173. Online ahead of print.ABSTRACTThe relative rarity of ovarian granulosa cell tumor (GCT) cases and preclinical models for GCT has posed a challenge for understanding GCT and developing therapeutics. Metabolic reprogramming is one of the key disruptions related to cancer progression. GCT exhibits significant lipid accumulation and overexpression of peroxisome proliferator-activated receptor gamma (PPARγ), a regulator of lipid metabolism. However, their roles remain unclear. Previously, we reported spontaneous GCT development in female mice with a constitutively ...
Source: Biology of Reproduction - December 20, 2023 Category: Reproduction Medicine Authors: Seok-Yeong Yu Yi Luan Pauline C Xu Yaqi Zhang Rosemary Dong Amirhossein Abazarikia So-Youn Kim Source Type: research
Cancers, Vol. 15, Pages 5864: Relevance of Molecular Pathology for the Diagnosis of Sex Cord & ndash;Stromal Tumors of the Ovary: A Narrative Review
Cancers, Vol. 15, Pages 5864: Relevance of Molecular Pathology for the Diagnosis of Sex Cord–Stromal Tumors of the Ovary: A Narrative Review
Cancers doi: 10.3390/cancers15245864
Authors:
Alexis Trecourt
Marie Donzel
Nadjla Alsadoun
Fabienne Allias
Mojgan Devouassoux-Shisheboran
Ovarian sex cord–stromal tumors (SCSTs) account for 8% of all primary ovarian neo-plasms. Accurate diagnosis is crucial since each subtype has a specific prognostic and treatment. Apart from fibrosarcomas, stromal tumors are benign while sex cord tumors may recur, sometimes with a significant time to relapse. ...
Source: Cancers - December 15, 2023 Category: Cancer & Oncology Authors: Alexis Trecourt Marie Donzel Nadjla Alsadoun Fabienne Allias Mojgan Devouassoux-Shisheboran Tags: Review Source Type: research
Mathematical modeling predicts pathways to successful implementation of combination TRAIL-producing oncolytic virus and PAC-1 to treat granulosa cell tumors of the ovary
Cancer Biol Ther. 2023 Dec 31;24(1):2283926. doi: 10.1080/15384047.2023.2283926. Epub 2023 Nov 27.ABSTRACTThe development of new cancer therapies requires multiple rounds of validation from in vitro and in vivo experiments before they can be considered for clinical trials. Mathematical models assist in this preclinical phase by combining experimental data with human parameters to provide guidance about potential therapeutic regimens to bring forward into trials. However, granulosa cell tumors of the ovary lack a relevant mouse model, complexifying preclinical drug development for this rare tumor. To bridge this gap, we est...
Source: Cancer Biology and Therapy - November 27, 2023 Category: Cancer & Oncology Authors: Justin Le Sauteur-Robitaille Powel Crosley Mary Hitt Adrianne L Jenner Morgan Craig Source Type: research
Mathematical modeling predicts pathways to successful implementation of combination TRAIL-producing oncolytic virus and PAC-1 to treat granulosa cell tumors of the ovary
Cancer Biol Ther. 2023 Dec 31;24(1):2283926. doi: 10.1080/15384047.2023.2283926. Epub 2023 Nov 27.ABSTRACTThe development of new cancer therapies requires multiple rounds of validation from in vitro and in vivo experiments before they can be considered for clinical trials. Mathematical models assist in this preclinical phase by combining experimental data with human parameters to provide guidance about potential therapeutic regimens to bring forward into trials. However, granulosa cell tumors of the ovary lack a relevant mouse model, complexifying preclinical drug development for this rare tumor. To bridge this gap, we est...
Source: Cancer Biology and Therapy - November 27, 2023 Category: Cancer & Oncology Authors: Justin Le Sauteur-Robitaille Powel Crosley Mary Hitt Adrianne L Jenner Morgan Craig Source Type: research
FOXL2: a gene central to ovarian function
The FOXL2 (forkhead box L2) gene is located on chromosome 3 and encodes for forkhead box (FOX) family of transcription factors which play a critical role in various biological processes. Germline FOXL2 mutations have been identified in blepharophimosis/ptosis/epicanthus inversus syndrome. The somatic missense mutation in FOXL2 (FOXL2 C134W) is now known to be the defining molecular feature of adult-type granulosa cell tumour of the ovary, present in over 90% of cases of this tumour type. Immunohistochemistry for FOXL2 is used as a marker of sex cord-stromal differentiation. However, expression is not restricted to lesions ...
Source: Journal of Clinical Pathology - November 17, 2023 Category: Pathology Authors: Mubeen, A., Parra-Herran, C. Tags: Gene of the month Source Type: research
Cytomorphologic diagnosis of adult granulosa cell tumors at the metastatic sites with an emphasis on the cytologic mimics
CONCLUSIONS: The current study not only outlines the distinct cytologic attributes of AGCTs across various metastatic locations but also highlights its prevalent cytologic mimics. Additionally, it outlines key clinicopathologic traits that can aid in distinguishing and precisely diagnosing these tumors through cytological analysis.PMID:37854236 | PMC:PMC10579011 (Source: American Journal of Translational Research)
Source: American Journal of Translational Research - October 19, 2023 Category: Research Authors: Tarunpreet Saini Parikshaa Gupta Nalini Gupta Sreedhara B Chaluvashetty Source Type: research
Cytomorphologic diagnosis of adult granulosa cell tumors at the metastatic sites with an emphasis on the cytologic mimics
CONCLUSIONS: The current study not only outlines the distinct cytologic attributes of AGCTs across various metastatic locations but also highlights its prevalent cytologic mimics. Additionally, it outlines key clinicopathologic traits that can aid in distinguishing and precisely diagnosing these tumors through cytological analysis.PMID:37854236 | PMC:PMC10579011 (Source: Cell Research)
Source: Cell Research - October 19, 2023 Category: Cytology Authors: Tarunpreet Saini Parikshaa Gupta Nalini Gupta Sreedhara B Chaluvashetty Source Type: research
