Modes of assay interference and the effectiveness of serum pretreatment approaches in detection of anti-HLA antibodies
Conclusions
Our data indicate that a single-method approach is insufficient to address all sources of interference in HLA SAB. A multimodal approach with a proactive screening is a more effective way to minimise risk of erroneous results. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Abraha, J., Rao, P., Morris, G. P. Tags: Original research Source Type: research
Implementation of an ISO15189 accredited next-generation sequencing service with the fully automated Ion Torrent Genexus: the experience of a clinical diagnostic laboratory
Conclusion
Successful implementation, clinical validation and accreditation of a novel NGS technology was achieved in this institution, with a significantly improved TAT of results to oncologists (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Werner, R., Connolly, A., Bennett, M., Hand, C. K., Burke, L. Tags: Open access Original research Source Type: research
Successful demand management in diagnostic immunology testing
Conclusions
Embedding algorithms in the ordering software can be acceptable to clinicians and have a major and sustained impact on overuse or underuse of tests. The algorithms can be replicated by other hospital trusts. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Lilly, K., Proudlove, N., Bethune, C. Tags: Original research Source Type: research
Efficacy of postmortem CT and tissue sampling in establishing the cause of death in clinical practice: a prospective observational study
Conclusion
PMCT and PMS confer additional diagnostic value in establishing the COD. Shortcomings in detecting vascular occlusions and perfusion disorders and susceptibility to pulmonary postmortem changes could in future be improved by additional techniques. Both PMCT and PMS are feasible in clinical practice and an alternative when autopsy cannot be performed. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Mentink, M. G., Latten, B. G. H., Bakers, F. C. H., Mihl, C., Benali, F., Nelemans, P. J., Rennenberg, R. J. M. W., Koopmans, R. P., Bergmans, D. C. J. J., Kubat, B., Hofman, P. A. M. Tags: Original research Source Type: research
Non-deletional haemoglobin H (Hb H) disease morphologically masquerading as congenital dyserythropoietic anaemia type II: a diagnostic pitfall
Haemoglobin H (Hb H) disease is a form of alpha (α)-thalassaemia where three of the four α-globin gene alleles are deleted. Hb H is usually classified as deletional and non-deletional types; however, the interaction of these two types is also encountered. Clinically, the patients can have a phenotype of thalassaemia intermedia requiring occasional blood transfusion to mild anaemia. Deletional form of Hb H (--/-α) due to double heterozygosity of deletions causing α0-thalassaemia (both α-globin HBA1 and HBA2 gene deleted on the allele) and α+-thalassaemia (single α-globin gene deleti...
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Jamwal, M., Sreedharanunni, S., Taak, R., Singh, N., Chhabra, S., Kaur, J., Amatya, S., Sharma, P., Trehan, A., Das, R. Tags: PostScript Source Type: research
Tumour-to-tumour metastasis of breast cancer (donor) and clear cell renal cell carcinoma (recipient): a case report
A woman in her late 70s was referred to urology clinic to consider intervention for a biopsy-proven 24 mm WHO/International Society of Urological Pathology (ISUP) grade 1 clear cell renal cell carcinoma (CCRCC) that was diagnosed 4 years prior and managed with active surveillance up to that point. The patient had a history of right invasive breast cancer (ductal carcinoma) first diagnosed more than 20 years ago and treated with wide local excision and adjuvant radiotherapy. She developed breast cancer recurrence in the scalp and chest wall 3 years before and was treated with letrozole, with satisfactory disease control. Fo...
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Hau, J., Briosa Neves, J., Varia, M. Tags: PostScript Source Type: research
Kinase expression in angiomatoid fibrous histiocytoma: panTRK is commonly expressed in the absence of NTRK rearrangement
Angiomatoid fibrous histiocytoma (AFH) is a soft tissue tumour of intermediate (rarely metastasising) malignant potential, which harbours EWSR1/FUS gene fusions. These tumours can express anaplastic lymphoma kinase (ALK) in the absence of gene rearrangement or copy number alteration and can also coexpresses Pan-TRK immunohistochemistry (IHC). All EWSR1/FUS-rearranged AFH were retrieved from the files of three institutions and Pan-TRK (EPR17341), ALK and BRAF V600E IHC were performed. Fourteen AFH cases were identified, which included three cases of intracranial mesenchymal tumours with FET-CREB fusions. PanTRK and ALK posi...
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Vargas, A. C., Joy, C., Maclean, F. M., Bonar, F., Wong, D. D., Gill, A. J., Cheah, A. L. Tags: Short report Source Type: research
Impact of cellular morphology and three-tiered nuclear grade on progression of conventional renal cell carcinoma
Conclusions
The three-tiered grading predicts the progression of cRCC irrespectively of cytomorphology. However, the cytomorphology and necrosis show a good correlation with three-tiered grading in estimate disease progression. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Domonkos, L., Yusenko, M., Kovacs, G., Peterfi, L. Tags: Short report Source Type: research
Comparison of assessment of programmed death-ligand 1 (PD-L1) status in triple-negative breast cancer biopsies and surgical specimens
Conclusions
We demonstrate high accordance between biopsies and surgical specimens for SP263 and 22C3 scoring but less for SP142. Generally, biopsies are suitable for PD-L1 testing in TNBC but the appropriate assay, scoring and cut-off must be considered. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Noske, A., Steiger, K., Ballke, S., Kiechle, M., Oettler, D., Roth, W., Weichert, W. Tags: Open access Original research Source Type: research
Expression and correlation of PD-L1 and HER2 in oesophageal squamous cell carcinoma
Conclusions
PD-L1 expression was significantly positively correlated with HER2 expression in OSCC. The results provide valuable insight for the future application of HER2-targeted therapy combined with immunotherapy in OSCC. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Rong, L., Zhao, H., Li, Y., Jin, M., Lu, J. Tags: Original research Source Type: research
Risk assessment in pT1 colorectal cancer
Colorectal cancer (CRC) is a common malignancy worldwide and tumour stage is closely related to clinical outcome. A small but significant proportion of submucosal-invasive (ie, pT1) CRC are associated with regional lymph node metastases (LNM) and a worse prognosis. The likelihood of LNM in pT1 CRC needs to be balanced against the operative risk and costs of surgical resection when determining the best patient management. A wide range of histopathological and clinical factors may affect LNM risk in this setting. This script provides a comprehensive overview of the tumour and patient-associated features that have been linked...
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Norton, E. J., Bateman, A. C. Tags: Editor's choice Review Source Type: research
Gender distribution in surgical pathology journal publications and editorial boards
Conclusions
To our knowledge, this study is the first to document under-representation of women among authors and editorial boards of surgical pathology journals. While women representation has increased over time, predominance of men remains relative to workforce proportions. Our findings are comparable to those from other medical fields and prompt the need to investigate the underlying causes for this imbalance and implement strategies to promote diversity, equity and inclusion in academic surgical pathology. (Source: Journal of Clinical Pathology)
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Nguyen, M. A., Yousef, S., Gupta, R., McKenzie, C. Tags: Original research Source Type: research
Gender distribution in pathology journal editorial boards: a call to action
The article by Nguyen et al reveals that Pathology, like other medical specialties, has an underrepresentation of women authors,1–7 editorial board members7–10 and editors in chiefs7–11 (EICs) of our journals. While it would be easy to feel discouraged by these findings, instead we have opted to examine how their data reveal the power of community accountability to improve diversity. The authors’ data, in measures such as first authorship, middle authorship and editorial board composition, show that the percentage of women did not appear to change significantly between the first two time points, wit...
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Dry, S., Collins, L. C. Tags: Editorials Source Type: research
Gene of the month: DDIT3
DNA damage-inducible transcript 3 (DDIT3) gene, mapped to the human chromosome 12q13.3, encodes a protein that belongs to the CCAAT/enhancer-binding protein family of transcription factors. DDIT3 is involved in the proliferative control that responds to endoplasmic reticulum stress in normal conditions, dimerising other transcription factors with basic leucine zipper (bZIP) structural motifs. DDIT3 plays a significant role during cell differentiation, especially adipogenesis, arresting the maturation of adipoblasts. In disease, FUS/EWSR1::DDIT3 fusion is the pathogenic event that drives the development of myxoid liposarcom...
Source: Journal of Clinical Pathology - March 20, 2024 Category: Pathology Authors: Diaz-Perez, J. A., Kerr, D. A. Tags: Gene of the month Source Type: research
Pragmatic guide to the macroscopic evaluation of breast specimens
The pathological assessment of a breast surgical specimen starts with macroscopic evaluation, arguably one of the most critical steps, as only a small percentage of the tissue is examined microscopically. To properly evaluate and select tissue sections from breast specimens, it is essential to correlate radiological findings, prior biopsies, procedures and treatment with the gross findings. Owing to its fatty nature, breast tissue requires special attention for proper fixation to ensure appropriate microscopic evaluation and performance of ancillary studies. In addition, knowledge of the information necessary for patient m...
Source: Journal of Clinical Pathology - February 19, 2024 Category: Pathology Authors: Guzman-Arocho, Y. D., Collins, L. C. Tags: Review Source Type: research
