A precise temperature control during hyperthermic intraperitoneal chemotherapy promises an early return of bowel function.
This study is aimed to explore the role of a stable perfusion temperature in promoting bowel recovery of PC patients due to gastrointestinal malignancies. METHODS: Between January 2012 and July 2013, 59 PC patients undergoing cytoreductive surgery and three-cycle HIPEC were included. Patients having stable perfusion temperature for all cycles were assigned into the study group, with the rest having unstable temperature into the control group. Time of flatus and defecation passage and initiation time of enteral nutrition were compared between both groups to detect the significance in bowel function recovery, with visua...
Source: Cancer Biology and Therapy - July 10, 2020 Category: Cancer & Oncology Authors: Ye J, Chen L, Zuo J, Peng J, Chen C, Cai S, Song W, He Y, Yuan Y Tags: Cancer Biol Ther Source Type: research

DBDx-based drug combinations show highly potent therapeutic efficacy against human pancreatic cancer xenografts in athymic mice.
Abstract Previous studies have shown that DBDx, a combination consisting of dipyridamole, bestatin and dexamethasone is highly effective against several cancer xenografts in athymic mice. Here the therapeutic effects of DBDx and its combination with gemcitabine or capcitabine against human pancreatic cancer xenografts and the mechanism were studied. In vivo experiments performed in athymic mice showed that the antitumor efficacy of DBDx was much stronger than that of gemcitabine or capecitabine alone. Notably, the combination of DBDx and gemcitabine or capcitabine further enhanced the efficacy. In the case of DBDx...
Source: Cancer Biology and Therapy - July 10, 2020 Category: Cancer & Oncology Authors: Zheng YB, Zhang MR, Li Y, Liu XJ, Zhen YS Tags: Cancer Biol Ther Source Type: research

Silencing the intestinal GUCY2C tumor suppressor axis requires APC loss of heterozygosity.
Abstract Most sporadic colorectal cancer reflects acquired mutations in the adenomatous polyposis coli (APC) tumor suppressor gene, while germline heterozygosity for mutant APC produces the autosomal dominant disorder Familial Adenomatous Polyposis (FAP) with a predisposition to colorectal cancer. In these syndromes, loss of heterozygosity (LOH) silences the remaining normal allele of APC, through an unknown mechanism, as the initiating step in transformation. Guanylyl cyclase C receptor (GUCY2C) and its hormones, uroguanylin and guanylin, have emerged as a key signaling axis opposing mutations driving intestinal ...
Source: Cancer Biology and Therapy - June 28, 2020 Category: Cancer & Oncology Authors: Pattison AM, Barton JR, Entezari AA, Zalewski A, Rappaport JA, Snook AE, Waldman SA Tags: Cancer Biol Ther Source Type: research

MiR-185-3p mimic promotes the chemosensitivity of CRC cells via AQP5.
Abstract BACKGROUND: Studies showed that microRNAs (miRNAs) are important regulators in drug resistance. The current study investigated the role of miR-185-3p and its predicted target gene AQP5 in 5-FU-insensitive colorectal cancer (CRC) cells. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) and Spearman's correlation analysis were conducted to determine the correlation of expression levels of miR-185-3p and AQP5 from CRC tissues. HCT-116 and HCT-8 cells were treated by gradient concentration of 5-FU to construct 5-FU-resistant CRC model. The inhibition and viability of 5-FU-resistant cell...
Source: Cancer Biology and Therapy - June 26, 2020 Category: Cancer & Oncology Authors: Zhou C, Kong W, Ju T, Xie Q, Zhai L Tags: Cancer Biol Ther Source Type: research

Dysregulated circRNA_100876 contributes to proliferation and metastasis of colorectal cancer by targeting microRNA-516b (miR-516b).
In conclusion, this study revealed that circRNA_100876 is overexpressed in CRC tissues and represents a promising therapeutic target for CRC. PMID: 32564659 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - June 21, 2020 Category: Cancer & Oncology Authors: Zhang J, Wang H, Wu K, Zhan F, Zeng H Tags: Cancer Biol Ther Source Type: research

Third-line sunitinib treatment in a VHL-mutated metastatic intrahepatic cholangiocarcinoma: a case report and literature review.
Abstract Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive malignancy with a poor prognosis. There is no standard treatment beyond first-line chemotherapy and no molecular-targeted drug approved for advanced iCCA. We herein present a case of a 46-y-old Asian iCCA patient with multiple metastases in lung, bone, and liver. The patient progressed rapidly after first- and second-line chemotherapy. According to next-generation sequencing result of somatic Von Hippel-Lindau (VHL) gene mutation, the patient was administered third-line sunitinib and obtained a relatively longer survival of 9 months after ...
Source: Cancer Biology and Therapy - June 18, 2020 Category: Cancer & Oncology Authors: Li X, Gao L, Zhang L, Sun H, Gou H Tags: Cancer Biol Ther Source Type: research

Clinical implication and usefulness of de novo EGFR T790M mutation in lung adenocarcinoma with EGFR-tyrosine kinase inhibitor sensitizing mutation.
Abstract In total, 102 cases diagnosed as lung adenocarcinoma with EGFR-tyrosine kinase inhibitor (TKI) sensitizing mutations (mEGFR) and had been treated with 1st ~ 2nd generation EGFR-TKI alone were enrolled for this study. De novo T790 M status was tested using the tissues at the initial diagnosis and positivity was defined as the ratio of T790 M/wild-type copies over 0.00294 by ddPCR. Seventy patients (68.6%) harbored the de novo T790 M. De novo T790 M was more frequently detected in cases with EGFR L858 R mutation than those with EGFR exon 19 deletion (E19d) mutations (P = 0...
Source: Cancer Biology and Therapy - June 16, 2020 Category: Cancer & Oncology Authors: Lee SH, Kim EY, Kim A, Chang YS Tags: Cancer Biol Ther Source Type: research

LINC00641/miR-4262/NRGN axis confines cell proliferation in glioma.
In this study, we focused on the function and mechanism of LINC00641 in glioma. First of all, we found that LINC00641 was expressed at a low level in glioma cell lines. Importantly, overexpression of LINC00641 prevented cell proliferation but enhanced cell apoptosis. Meanwhile, NRGN, a previously-reported downregulated mRNA in GBM, was disclosed as a tumor suppressor in glioma cells. Besides, we verified that NRGN could be positively regulated by LINC00641 in glioma cells. Moreover, the cellular distribution of LINC00641 was identified to be cytoplasmic. Therefore, bioinformatics analysis and mechanism experiments were car...
Source: Cancer Biology and Therapy - June 16, 2020 Category: Cancer & Oncology Authors: Yang J, Yu D, Liu X, Changyong E, Yu S Tags: Cancer Biol Ther Source Type: research

Chetomin, a Hsp90/HIF1 α pathway inhibitor, effectively targets lung cancer stem cells and non-stem cells.
This study advocates chetomin as a Hsp90/HIF1α pathway inhibitor and a potent, nontoxic NSCLC CSC-targeting molecule. PMID: 32489150 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - June 3, 2020 Category: Cancer & Oncology Authors: Min S, Wang X, Du Q, Gong H, Yang Y, Wang T, Wu N, Liu X, Li W, Zhao C, Shen Y, Chen Y, Wang X Tags: Cancer Biol Ther Source Type: research

Transcriptional information underlying the generation of CSCs and the construction of a nine-mRNA signature to improve prognosis prediction in colorectal cancer.
CONCLUSIONS: We developed a novel model that provides additional and powerful prognostic information beyond conventional clinicopathological factors for CRC survival prediction. It also provides new insight into the molecular mechanisms underlying the transition from normal tissues to CSCs and formation of tumor tissues. PMID: 32453965 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - May 26, 2020 Category: Cancer & Oncology Authors: Zheng W, Yang C, Qiu L, Feng X, Sun K, Deng H Tags: Cancer Biol Ther Source Type: research

Identification of potential therapeutic targets in urothelial bladder carcinoma of Chinese population by targeted next-generation sequencing.
Abstract Patients with urothelial carcinoma (UC) of the bladder have a high risk of death in China. However, a lack of comprehensive molecular profiling in Chinese Han population hinders the development of targeted therapies for bladder cancer. In our present study, we collected fresh bladder tumors from low-grade (T1, N0, M0, G1) non-muscle invasive bladder cancer (NMIBC) patients (n = 16) and high-grade (T2-4, N0, M0, Gx) muscle-invasive bladder cancer (MIBC) patients (n = 16) with their paired normal bladder tissues, and subjected the total genomic DNAs to targeted next-generation sequencing...
Source: Cancer Biology and Therapy - May 23, 2020 Category: Cancer & Oncology Authors: Wang T, Liu Z, Wang X, Bai P, Sun A, Shao Z, Luo R, Wu Z, Zhang K, Li W, Xiao W, Duan B, Wang Y, Chen B, Xing J Tags: Cancer Biol Ther Source Type: research

Exosomal taurine up-regulated 1 promotes angiogenesis and endothelial cell proliferation in cervical cancer.
Abstract Emerging evidence had highlighted that exosomes could mediate cell-cell communication in human cancerous development via transferring the various molecular cargos, including long non-coding RNA (lncRNA). Taurine up-regulated 1 (TUG1) was previously reported as an oncogenic lncRNA in cervical cancer (CC) via facilitating cell proliferation and other vital biological behaviors. Nevertheless, the presence of TUG1 in exosomes and the functional regulation of exosomal TUG1 in CC are still elusive. The current study aimed at the communication between CC cell lines and endothelial cell-mediated by exosomes, as w...
Source: Cancer Biology and Therapy - May 20, 2020 Category: Cancer & Oncology Authors: Lei L, Mou Q Tags: Cancer Biol Ther Source Type: research

ST8SIA6-AS1 promotes hepatocellular carcinoma by absorbing miR-5195-3p to regulate HOXB6.
Conclusions: To sum up, ST8SIA6-AS1 promotes HCC progression by absorbing miR-5195-3p to regulate HOXB6, which might provide some worthy suggestions to research the development process of HCC. PMID: 32420798 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - May 18, 2020 Category: Cancer & Oncology Authors: Li Y, Jiang A Tags: Cancer Biol Ther Source Type: research

The treatment option of progressive disease in breast cancer during neoadjuvant chemotherapy: a single-center experience.
Abstract Patients' responses to breast cancer neoadjuvant chemotherapy (NACT) differ because of heterogeneous tumor characteristics. Reports about NACT progression are sporadic. Here we enrolled 1187 patients who received NACT in our cancer center between January 1, 2007, and December 31, 2016. We analyzed the characteristics and treatments of patients with progressive disease (PD) or non-PD or pathological complete response (pCR). In total, 45 (3.8%) patients had PD. PD patients were associated with a significantly worse disease-free survival (DFS) (hazard ratio (HR) = 3.77; 95% CI, 1.77 to 8.00; P =.00...
Source: Cancer Biology and Therapy - May 18, 2020 Category: Cancer & Oncology Authors: Zheng Y, Ding X, Zou D, Zhang F, Qin C, Yang H, Mo W, Ding Y, Yu Y Tags: Cancer Biol Ther Source Type: research

STAT4-mediated down-regulation of miR-3619-5p facilitates stomach adenocarcinoma by modulating TBC1D10B.
Conclusion: STAT4-mediated miR-3619-5p controls STAD carcinogenesis and progression through modulating TBC1D10B expression, which may provide a novel insight for researching the STAD-related molecular mechanism. PMID: 32397798 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - May 13, 2020 Category: Cancer & Oncology Authors: Liu Y, Li J, Wang S, Song H, Yu T Tags: Cancer Biol Ther Source Type: research

Malignant cell-specific pro-tumorigenic role of type I interferon receptor in breast cancers.
Abstract Within the microenvironment of solid tumors, stress associated with deficit of nutrients and oxygen as well as tumor-derived factors triggers the phosphorylation-dependent degradation of the IFNAR1 chain of type I interferon (IFN1) receptor and ensuing suppression of the IFN1 pathway. Here we sought to examine the importance of these events in malignant mammary cells. Expression of non-degradable IFNAR1S526A mutant in mouse mammary adenocarcinoma cells stimulated the IFN1 pathway yet did not affect growth of these cells in vitro or ability to form subcutaneous tumors in the syngeneic mice. Remarkably, the...
Source: Cancer Biology and Therapy - May 7, 2020 Category: Cancer & Oncology Authors: Odnokoz O, Yu P, Peck AR, Sun Y, Kovatich AJ, Hooke JA, Hu H, Mitchell EP, Rui H, Fuchs SY Tags: Cancer Biol Ther Source Type: research

Copy number variation of ubiquitin- specific proteases genes in blood leukocytes and colorectal cancer.
Abstract Ubiquitin-specific proteases (USPs) play important roles in the regulation of many cancer-related biological processes. USPs copy number variation (CNVs) may affect the risk and prognosis of colorectal cancer (CRC). We detected CNVs of USPs genes in 468 matched CRC patients and controls, estimated the associations between the USPs genes CNVs and CRC risk and prognosis and their interactions with environmental factors on CRC risk. Finally, we generated five CRC risk predictive models with different CNVs patterns combining with environmental factors (EF). We identified significant association between CYLD d...
Source: Cancer Biology and Therapy - May 4, 2020 Category: Cancer & Oncology Authors: Tian T, Bi H, Liu Y, Li G, Zhang Y, Cao L, Hu F, Zhao Y, Yuan H Tags: Cancer Biol Ther Source Type: research

LINC01128 resisted acute myeloid leukemia through regulating miR-4260/NR3C2.
In conclusion, we firstly uncovered that LINC01128 resisted acute myeloid leukemia through regulating miR-4260/NR3C2, providing novel clues for the treatment improvement of AML. PMID: 32338183 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - April 26, 2020 Category: Cancer & Oncology Authors: Li H, Tian X, Wang P, Hu J, Qin R, Xu R, Liu K, Hao J, Tian N Tags: Cancer Biol Ther Source Type: research

LncRNA OIP5-AS1 promotes cell proliferation and migration and induces angiogenesis via regulating miR-3163/VEGFA in hepatocellular carcinoma.
This study aims to explore the inherent mechanism of lncRNA OIP5-AS1 in HCC. In the first place, qRT-PCR found that OIP5-AS1 and VEGFA expressions were significantly increased while miR-3163 was obviously reduced in HCC cells and tissues. Next, a series of functional experiments found that knockdown of OIP5-AS1 suppressed HCC cell proliferation, migration and angiogenesis abilities while promoting cell apoptosis simultaneously. Last but not least, miR-3163 inhibition or VEGFA overexpression can reverse the anti-tumor effect of OIP5-AS1. In summary, OIP5-AS1 affects HCC proliferation, metastasis, and angiogenesis in HCC by ...
Source: Cancer Biology and Therapy - April 24, 2020 Category: Cancer & Oncology Authors: Shi C, Yang Q, Pan S, Lin X, Xu G, Luo Y, Zheng B, Xie X, Yu M Tags: Cancer Biol Ther Source Type: research

Analysis of long-term outcome of image-guided volumetric modulated arc therapy (VMAT) for primary malignant tumor of the cervical spine.
Abstract Objective: Retrospective analysis of the long-term clinical outcome and acute toxicity of the primary malignant tumor of cervical spine receiving CBCT image-guided VMAT.Methods: Thirty patients with primary malignant tumor of the cervical spine included in our center, from December 2013 to January 2016, 28 patients were retrospectively studied. The prescription dosage 95% PTV volume dose was 44 Gy, 2.0 Gy/fraction, and a total of 22 times. The median PGTV synchronized volume dose was 60 Gy (45-62.1 Gy), median 2.5 Gy (2-2.7 Gy)/fraction. In volumetric modulated, two arc volum...
Source: Cancer Biology and Therapy - April 16, 2020 Category: Cancer & Oncology Authors: Jiang P, Zhang X, Jiang W, Meng N, Aili A, Wang J Tags: Cancer Biol Ther Source Type: research

A novel targeted GPC3/CD3 bispecific antibody for the treatment hepatocellular carcinoma.
Abstract Hepatocellular carcinoma (HCC) is the most frequent primary liver cancer but has shown limited success to date in the treatment of advanced stage. Recruitment of T cells for cancer treatment is a rapidly growing strategy in immunotherapy such as chimeric antigen receptor T cells and bispecific antibodies. However, unwanted aggregations, structural instability or short serum half-life are major challenges of bispecific antibodies. Here, we developed a new format of T cell-redirecting antibody that is bispecific for membrane proteoglycans GPC3 of HCC and the T-cell-specific antigen CD3, which demonstrated t...
Source: Cancer Biology and Therapy - April 2, 2020 Category: Cancer & Oncology Authors: Yu L, Yang X, Huang N, Lang QL, He QL, Jian-Hua W, Liang-Peng G Tags: Cancer Biol Ther Source Type: research

Significant response to apatinib monotherapy in heavily pretreated advanced HER2-positive breast cancer: a case report and literature review.
Abstract Although HER2-targeted therapy has been shown to prolong the survival of patients with HER2-positive breast cancer, most patients eventually progress due to drug resistance. Novel treatment options are urgently needed to overcome resistance to HER2-targeted therapy. The VEGF/VEGFR (Vascular endothelial growth factor and its receptors) pathway is essential in tumor angiogenesis, which may be a promising target in HER2-positive breast cancer providing a rationale for the use of tyrosine kinase inhibitors (TKIs) targeting VEGFR. Here, we present a case of a heavily pretreated advanced breast cancer patient w...
Source: Cancer Biology and Therapy - April 1, 2020 Category: Cancer & Oncology Authors: Danni L, Lingyun Z, Jian W, Hongfei Y, Lu X, Peng Y, Xiujuan Q, Yunpeng L, Yuee T Tags: Cancer Biol Ther Source Type: research

Heat shock proteins as biomarkers of lung cancer.
Abstract Heat shock proteins are known to be associated with a wide variety of human cancers including lung cancer. Overexpression of these molecular chaperones is linked with tumor survival, metastasis and anticancer drug resistance. In recent years, heat shock proteins are gaining much importance in the field of cancer research owing to their potential to be key determinants of cell survival and apoptosis. Lung cancer is one of the most common cancers diagnosed worldwide and the association of heat shock proteins in lung cancer diagnosis, prognosis and as drug targets remains unresolved. The aim of this review i...
Source: Cancer Biology and Therapy - March 31, 2020 Category: Cancer & Oncology Authors: Mittal S, Rajala MS Tags: Cancer Biol Ther Source Type: research

Rules for scientific progress while living with the COVID-19 Pandemic: from 'benchside' to 'fireside.'
Authors: PMID: 32223689 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - March 30, 2020 Category: Cancer & Oncology Tags: Cancer Biol Ther Source Type: research

Cytokines secreted by stromal cells in TNBC microenvironment as potential targets for cancer therapy.
In this study, we performed a systematic analysis of cytokine factors secreted from four stromal components (fibroblasts, macrophages, lymphatic endothelial cells, and blood microvascular endothelial cells) induced by four TNBC cell types. Through bioinformatic analysis, we selected putative candidates of secreted factors from stromal cells, which are involved in EMT activity, cell proliferation, metabolism, and matrisome pathways. Among the candidates, LCN2, GM-CSF, CST3, IL-6, IL-8, and CHI3L1 are ranked highly. Significantly, Lipocalin-2 (LCN2) is upregulated in the crosstalk of stromal cells and four different TNBC cel...
Source: Cancer Biology and Therapy - March 26, 2020 Category: Cancer & Oncology Authors: Malone MK, Smrekar K, Park S, Blakely B, Walter A, Nasta N, Park J, Considine M, Danilova LV, Pandey NB, Fertig EJ, Popel AS, Jin K Tags: Cancer Biol Ther Source Type: research

Apatinib in recurrent anaplastic meningioma: a retrospective case series and systematic literature review.
Conclusion: Apatinib is actively used in treating patients with recurrent AM. A randomized trial and phase II clinical trial of this inhibitor should be performed to further evaluate its efficacy in treating malignant meningioma. PMID: 32212907 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - March 25, 2020 Category: Cancer & Oncology Authors: Wang Y, Li W, Jing N, Meng X, Zhou S, Zhu Y, Xu J, Tao R Tags: Cancer Biol Ther Source Type: research

Chimeric cytokine receptor enhancing PSMA-CAR-T cell-mediated prostate cancer regression.
Conclusions: PSMA-CAR-T cells co-expressing ICR can be envisaged as a new therapeutic strategy for prostate cancer and support the translation of this enhanced approach in the clinical setting. PMID: 32208880 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - March 25, 2020 Category: Cancer & Oncology Authors: Weimin S, Abula A, Qianghong D, Wenguang W Tags: Cancer Biol Ther Source Type: research

Isolated pineal gland metastasis of acute lymphocytic leukemia: case report.
Abstract Metastasis in the pineal region is a rare condition. To best of our knowledge, there is no case report of isolated pineal metastasis secondary to acute lymphocytic leukemia (ALL). The aim of this study is to show the pineal gland involvement of ALL in a case for the first time in the literature. A 25-year-old male patient diagnosed with ALL 2 years ago presented with headache and visual impairment. Brain magnetic resonance imaging (MRI) revealed a well-defined solid lesion which was revealed intensive enhancement after contrast. On diffusion-weighted images, the lesion showed significant diffusion r...
Source: Cancer Biology and Therapy - March 25, 2020 Category: Cancer & Oncology Authors: Taydas O, Yesilyurt M, Ogul Y, Ogul H Tags: Cancer Biol Ther Source Type: research

Patient-derived cancer modeling for precision medicine in colorectal cancer: beyond the cancer cell line.
Abstract Since effective immunotherapeutic agents such as immune checkpoint blockade to treat cancer have emerged, the need for reliable preclinical cancer models that can evaluate and discover such drugs became stronger than ever before. The traditional preclinical cancer model using a cancer cell line has several limitations to recapitulate intra-tumor heterogeneity and in-vivo tumor activity including interactions between tumor-microenvironment. In this review, we will go over various preclinical cancer models recently discovered including patient-derived xenografts, humanized mice, organoids, organotypic-tumor...
Source: Cancer Biology and Therapy - March 25, 2020 Category: Cancer & Oncology Authors: Pyo DH, Hong HK, Lee WY, Cho YB Tags: Cancer Biol Ther Source Type: research

A novel non-agonist c-Met antibody drug conjugate with superior potency over a c-Met tyrosine kinase inhibitor in c-Met amplified and non-amplified cancers.
Abstract c-Met is a well-characterized oncogene that is associated with poor prognosis in many solid tumor types. While responses to c-Met inhibitors have been observed in clinical trials, activity appears to be limited to those with MET gene amplifications or mutations. We developed a c-Met targeted antibody-drug conjugate (ADC) with preclinical activity in the absence of MET gene amplification or mutation, and activity even in the context of moderate protein expression. The ADC utilized a high-affinity c-Met antibody (P3D12), that induced c-Met degradation with minimal activation of c-Met signaling, or mitogenic...
Source: Cancer Biology and Therapy - March 19, 2020 Category: Cancer & Oncology Authors: Fujita R, Blot V, Wong E, Stewart C, Lieuw V, Richardson R, Banah A, Villicana J, Timmer A, Coronella J, Newman R, Gymnopoulos M Tags: Cancer Biol Ther Source Type: research

The antilymphatic metastatic effect of hyaluronic acid in a mouse model of oral squamous cell carcinoma.
Conclusion: Our findings confirmed the establishment of a fast and reliable lymphatic metastatic mouse model of OSCC that can be used for investigating metastatic mechanisms and analyzing various antimetastasis strategies. An equally important discovery is the antimetastatic property of HA, which could provide a potential therapeutic strategy for OSCC. PMID: 32186431 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - March 18, 2020 Category: Cancer & Oncology Authors: Chen H, Wang M, Qi M, Tian Z, Wu W, Yang J, Zhang M, Tang L, Tang X Tags: Cancer Biol Ther Source Type: research

The histone demethylase KDM2B activates FAK and PI3K that control tumor cell motility.
Abstract Recent studies revealed that the histone demethylase KDM2B regulates the epithelial markers E-Cadherin and ZO-1, the RhoA/B/C-small-GTPases and actin cytoskeleton organization, in DU-145 prostate- and HCT-116 colon-tumor cells. Here we addressed the role of KDM2B in the activation of Focal Adhesion Kinase (FAK)-signaling and its involvement in regulating tumor cell motility. We used RT-PCR for gene transcriptional analysis, Western blotting for the assessment of protein expression and activity and wound-healing assay for the study of cell migration. KDM2B overexpression or silencing controls the activity ...
Source: Cancer Biology and Therapy - March 16, 2020 Category: Cancer & Oncology Authors: Zacharopoulou N, Kallergi G, Alkahtani S, Tsapara A, Alarifi S, Schmid E, Sukkar B, Kampranis S, Lang F, Stournaras C Tags: Cancer Biol Ther Source Type: research

Prospects of NSAIDs administration as double-edged agents against endometrial cancer and pathological species of the uterine microbiome.
Abstract Many types of cancers, including endometrial cancer, were found to have cyclooxygenase-2 (COX-2) overexpression. Because this enzyme belongs to the group of pro-inflammatory enzymes, so-called NSAIDs (non-steroidal anti-inflammatory drugs) directly inhibit its activity. An increasing number of reports on COX-2 involvement in cancer, as well as on the role of microbiota in abnormal metabolism and signaling of cells, forces the development of new NSAID types. Besides, NSAIDs can affect some bacteria, which are vaginal/endometrial microbiome members. The overgrowth of those species was found to be a major ca...
Source: Cancer Biology and Therapy - March 15, 2020 Category: Cancer & Oncology Authors: Kuźmycz O, Stączek P Tags: Cancer Biol Ther Source Type: research

Phosphorylated STAT3 (Tyr705) as a biomarker of response to pimozide treatment in triple-negative breast cancer.
Abstract Triple-negative breast cancer (TNBC) displays an aggressive clinical course, heightened metastatic potential, and is linked to poor survival rates. Through its lack of expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), this subtype remains unresponsive to traditional targeted therapies. Undesirable and sometimes life-threatening side effects associated with current chemotherapeutic agents warrant the development of more targeted treatment options. Targeting signal transducer and activator of transcription 3 (STAT3), a transcription fa...
Source: Cancer Biology and Therapy - March 13, 2020 Category: Cancer & Oncology Authors: Dees S, Pontiggia L, Jasmin JF, Mercier I Tags: Cancer Biol Ther Source Type: research

MiR-324-5p/PTPRD/CEBPD axis promotes papillary thyroid carcinoma progression via microenvironment alteration.
In this study, we investigated the interaction between miR-324-5p/PTPRD/CEBPD axis and tumor microenvironment in PTC progression. K1 and KTC-1 were transfected by lenti-CEBPD or CEBPD-sh vectors. Supernatant from different groups was harvested and added into culture media of human macrophages and HUVEC. Cell viability, colony formation, invasive and migrated cell number, and gap closure rate were elevated in lenti-CEBPD group. Compared with the control, supernatant from lenti-CEBPD group contained more abundant levels of VEGF and IL-4/IL-13, which, respectively, induced higher HUVEC invasion/migration rates and more obviou...
Source: Cancer Biology and Therapy - March 10, 2020 Category: Cancer & Oncology Authors: Yang Y, Xia S, Zhang L, Wang W, Chen L, Zhan W Tags: Cancer Biol Ther Source Type: research

Overall tumor genomic instability: an important predictor of recurrence-free survival in patients with localized clear cell renal cell carcinoma.
Abstract Measurement of a tumor's overall genomic instability has gathered recent interest over the identification of specific genomic imbalances, as it may provide a more robust measure of tumor aggressiveness. Here we demonstrate the association of tumor genomic instability in the prediction of disease recurrence in patients with clinically localized clear cell renal cell carcinoma (ccRCC). Genomic copy number analysis was performed using SNP-based microarrays on tumors from 103 ccRCC patients. The number of copy number alterations (CNAs) for each tumor was calculated, and a genomic imbalance threshold (GIT) ass...
Source: Cancer Biology and Therapy - March 1, 2020 Category: Cancer & Oncology Authors: Correa AF, Ruth KJ, Al-Saleem T, Pei J, Dulaimi E, Kister D, Collins M, Abbosh PH, Slifker MJ, Ross E, Uzzo RG, Testa JR Tags: Cancer Biol Ther Source Type: research

Neoadjuvant chemotherapy is associated with a transient increase of intratumoral T-cell density in microsatellite stable colorectal liver metastases.
In this study, densities of total-, cytotoxic-, helper- and regulatory T-cells were quantified by immunohistochemistry in resected CLM from 92 patients included in the OSLO-COMET trial (NCT01516710). All but one patient had microsatellite stable tumors (91/92). Associations between T-cell densities and clinicopathological parameters were analyzed. Fluoropyrimidine-based NACT (in most cases with addition of oxaliplatin or irinotecan) was administered to 45 patients completed median 8 weeks prior to surgical resection. No overall association was found between NACT administration and intratumoral T-cell densities. Howeve...
Source: Cancer Biology and Therapy - February 26, 2020 Category: Cancer & Oncology Authors: Dagenborg VJ, Marshall SE, Yaqub S, Grzyb K, Boye K, Lund-Iversen M, Høye E, Berstad AE, Fretland ÅA, Edwin B, Ree AH, Flatmark K Tags: Cancer Biol Ther Source Type: research

Correction.
Authors: PMID: 32093521 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - February 24, 2020 Category: Cancer & Oncology Tags: Cancer Biol Ther Source Type: research

Knockdown of lncRNA CCAT1 enhances sensitivity of paclitaxel in prostate cancer via regulating miR-24-3p and FSCN1.
Abstract Drug resistance limits the efficacy of chemotherapy in human cancers. Previous studies reported that long noncoding RNA colon cancer-associated transcript 1 (CCAT1) regulated progression of prostate cancer (PCa). However, the potential role of CCAT1 in the sensitivity of paclitaxel (PTX) in PCa and its mechanism remain largely unknown. The PTX-resistant PCa cells were established in PC3 and DU145 cells by increasing concentrations of PTX. The expressions of CCAT1, microRNA-24-3p (miR-24-3p) and fascin1 (FSCN1) were measured by quantitative real-time polymerase chain reaction. The viability and apoptosis w...
Source: Cancer Biology and Therapy - February 23, 2020 Category: Cancer & Oncology Authors: Li X, Han X, Wei P, Yang J, Sun J Tags: Cancer Biol Ther Source Type: research

Combined targeting of EGFR and HER2 against prostate cancer stem cells.
Abstract Progression of prostate cancer has been associated with EGFR and HER2 activation and to tumor-initiating cells contribution toward chemotherapy resistance. We investigated the efficacy of a dual intervention against EGFR and HER2 to deplete the tumor-initiating cells, optimize the chemotherapy management and prevent the progression of castration-resistant prostate cancer (CRPC) cells. Using DU145, PC3, and 22Rv1 CRPC cell lines, biochemical analysis revealed activation of EGFR, HER2, MAPK, and STAT3 in DU145 and 22Rv1, and AKT and SRC in DU145 and PC-3. pSTAT3 nuclear staining was observed in DU145 xenogr...
Source: Cancer Biology and Therapy - February 23, 2020 Category: Cancer & Oncology Authors: Rossini A, Giussani M, Ripamonti F, Aiello P, Regondi V, Balsari A, Triulzi T, Tagliabue E Tags: Cancer Biol Ther Source Type: research

Harnessing the vulnerabilities of p53 mutants in lung cancer - Focusing on the proteasome: a new trick for an old foe?
Abstract Gain-of-function (GOF) p53 mutations occur commonly in human cancer and lead to both loss of p53 tumor suppressor function and acquisition of aggressive cancer phenotypes. The oncogenicity of GOF mutant p53 is highly related to its abnormal protein stability relative to wild type p53, and overall stoichiometric excess. We provide an overview of the mechanisms of dysfunction and abnormal stability of GOF p53 specifically in lung cancer, the leading cause of cancer-related mortality, where, depending on histologic subtype, 33-90% of tumors exhibit GOF p53 mutations. As a distinguishing feature and oncogenic...
Source: Cancer Biology and Therapy - February 10, 2020 Category: Cancer & Oncology Authors: Oduah EI, Grossman SR Tags: Cancer Biol Ther Source Type: research

The stress-activated protein kinase pathway and the expression of stanniocalcin-1 are regulated by miR-146b-5p in papillary thyroid carcinogenesis.
This study explores the hypothesis that miR-146b-5p contributes to papillary thyroid carcinogenesis through regulation of cell signaling pathways in a manner that overcomes the cellular growth suppressive events and provides survival advantage. The effect of miR-146b-5p inhibition on major cancer related signaling pathways and expression of Stanniocalcin-1 (STC1), an emerging molecule associated with stress response and carcinogenesis, was tested in cultured primary thyroid cells using luciferase reporter assays, quantitative real-time PCR, immunofluorescence staining, and flow cytometry. Our results demonstrated that miR-...
Source: Cancer Biology and Therapy - February 9, 2020 Category: Cancer & Oncology Authors: Al-Abdallah A, Jahanbani I, Mehdawi H, Ali RH, Al-Brahim N, Mojiminiyi O Tags: Cancer Biol Ther Source Type: research

APC- β-catenin-TCF signaling silences the intestinal guanylin-GUCY2C tumor suppressor axis.
APC-β-catenin-TCF signaling silences the intestinal guanylin-GUCY2C tumor suppressor axis. Cancer Biol Ther. 2020 Feb 09;:1-11 Authors: Blomain ES, Rappaport JA, Pattison AM, Bashir B, Caparosa E, Stem J, Snook AE, Waldman SA Abstract Sporadic colorectal cancer initiates with mutations in APC or its degradation target β-catenin, producing TCF-dependent nuclear transcription driving tumorigenesis. The intestinal epithelial receptor, GUCY2C, with its canonical paracrine hormone guanylin, regulates homeostatic signaling along the crypt-surface axis opposing tumorigenesis. Here, we reveal that e...
Source: Cancer Biology and Therapy - February 9, 2020 Category: Cancer & Oncology Authors: Blomain ES, Rappaport JA, Pattison AM, Bashir B, Caparosa E, Stem J, Snook AE, Waldman SA Tags: Cancer Biol Ther Source Type: research

Doxycycline-induced exogenous Bmi-1 expression enhances tumor formation in a murine model of oral squamous cell carcinoma.
Abstract B Cell-Specific Moloney Murine Leukemia Virus Integration Site 1 (Bmi-1, Bmi1), an epigenetic protein, is necessary for normal stem cell self-renewal in adult animals and for cancer stem cell (CSC) functions in adult animals. To elucidate the functions of Bmi-1 in the oral cavity we created a transgenic mouse line (KrTBmi-1) that expresses ectopic, Flag-tagged Bmi-1 in tongue basal epithelial stem cells only upon doxycycline (DOX) treatment. Genome wide transcriptomics and Ingenuity Pathway Analysis identified several pathways altered by exogenous Bmi-1 expression in the normal tongue epithelium, includin...
Source: Cancer Biology and Therapy - February 9, 2020 Category: Cancer & Oncology Authors: Kalish JM, Tang XH, Scognamiglio T, Zhang T, Gudas LJ Tags: Cancer Biol Ther Source Type: research

John Mendelsohn's journey in cancer biology and therapy.
PMID: 31971860 [PubMed - as supplied by publisher] (Source: Cancer Biology and Therapy)
Source: Cancer Biology and Therapy - January 23, 2020 Category: Cancer & Oncology Authors: Kumar R Tags: Cancer Biol Ther Source Type: research

Identification of a minimal region of loss on chromosome 6q27 associated with poor survival of high-risk neuroblastoma patients.
olo A Abstract Patients with high-risk neuroblastoma (HR-NB) often initially respond to therapy, but afterward they become resistant and disease recurred. Unfortunately, it does not exist one or more specific chromosome defects associated with relapse or refractory NB. Recently, genomic evidence from primary tumors indicated that the distal region of chromosome 6q is loss in HR-NB patients with fatal outcome. We identified a minimal common region of loss of chromosome 6q27 spanning an area of 2.09 Mb by high-resolution DNA copy number data of a small cohort of HR-NB samples carrying 6q loss. This region of loss ha...
Source: Cancer Biology and Therapy - January 20, 2020 Category: Cancer & Oncology Authors: Ognibene M, Morini M, Garaventa A, Podestà M, Pezzolo A Tags: Cancer Biol Ther Source Type: research

Identification of a new cryptic PML-RAR α fusion gene without t(15;17) and biallelic CEBPA mutation in a case of acute promyelocytic leukemia: a case detected only by RT-PCR but not cytogenetics and FISH.
Identification of a new cryptic PML-RARα fusion gene without t(15;17) and biallelic CEBPA mutation in a case of acute promyelocytic leukemia: a case detected only by RT-PCR but not cytogenetics and FISH. Cancer Biol Ther. 2020 Jan 20;:1-6 Authors: Zhang Z, Xu Y, Jiang M, Kong F, Chen Z, Liu S, Li F Abstract Acute promyelocytic leukemia (APL) is characterized by the presence of promyelocytic leukemia-retinoic acid receptor α (PML-RARα) fusion gene, which is formed following the specific chromosomal translocation t(15;17)(q22;q21). However, cases with PML-RARα generated by occult...
Source: Cancer Biology and Therapy - January 20, 2020 Category: Cancer & Oncology Authors: Zhang Z, Xu Y, Jiang M, Kong F, Chen Z, Liu S, Li F Tags: Cancer Biol Ther Source Type: research

PRKCZ-AS1 promotes the tumorigenesis of lung adenocarcinoma via sponging miR-766-5p to modulate MAPK1.
In this study, the expression of PRKCZ-AS1 in LUAD tissues and cell lines was notably upregulated. Moreover, knockdown of PRKCZ-AS1 inhibited the proliferation and migration, but promoted apoptosis in LUAD cells. Furthermore, miR-766-5p could bind with PRKCZ-AS1. Besides, the expression miR-766-5p was negatively regulated by PRKCZ-AS1 expression in LUAD cells. Furtherly, PRKCZ-AS1 expression positively regulated the expression of MAPK1. Similarly, the expression of MAPK1 was negatively regulated by miR-766-5p expression. Moreover, the binding ability between miR-766-5p and MAPK1 was confirmed. Furthermore, knockdown of MAP...
Source: Cancer Biology and Therapy - January 15, 2020 Category: Cancer & Oncology Authors: Wang M, Liao Q, Zou P Tags: Cancer Biol Ther Source Type: research

SIRT6-PARP1 is involved in HMGB1 polyADP-ribosylation and acetylation and promotes chemotherapy-induced autophagy in leukemia.
In this study, we showed that knockdown of SIRT6 or PARP1 gene expression significantly inhibited HMGB1 cytoplasmic translocation and autophagy. Meanwhile, we found that SIRT6, an important upstream protein of PARP1, associated with PARP1, leading to the stimulation of polyADP-ribose polymerase activity. We further demonstrated that SIRT6 and PARP1 activation were required for chemotherapy-induced ADP-ribosylation of HMGB1 in leukemic cells and then influenced the acetylation of HMGB1, finally promoting the autophagy of leukemic cells mediated by HMGB1 translocation. These findings provide new insights into the mechanism o...
Source: Cancer Biology and Therapy - January 13, 2020 Category: Cancer & Oncology Authors: Kong Q, Li Y, Liang Q, Xie J, Li X, Fang J Tags: Cancer Biol Ther Source Type: research

Ibrutinib resistance in a patient with transformed diffuse large B-cell lymphoma from primary pulmonary mucosa-associated lymphoid tissue lymphoma.
We report a 55 year-old woman with a 3 year history of primary pulmonary MALT lymphoma confined to the lung presenting with massive pleural effusion. After two cycles of R-CHOP and six cycles of R2-CHOP, pleural effusion disappeared but the pulmonary mass remained persistent. Second-line therapies R2-GemOx failed to make any substantial improvement. Core-needle puncture biopsy of the pulmonary mass was obtained and pathological testing revealed transformed diffuse large B-cell lymphoma of germinal center B-cell subtype. Next-generation sequencing confirmed BN2 subtype. The mass showed no reduction after three cycles of R-M...
Source: Cancer Biology and Therapy - January 13, 2020 Category: Cancer & Oncology Authors: Zhou H, Yang L, Dang Q, Huang J, Cheng Y, Zhang Y, Shi W Tags: Cancer Biol Ther Source Type: research