Use of CRISPR interference for efficient and rapid gene inactivation in < em > Fusobacterium nucleatum < /em >
Appl Environ Microbiol. 2024 Jan 8:e0166523. doi: 10.1128/aem.01665-23. Online ahead of print.ABSTRACTHow can we effectively investigate the gene functions in Fusobacterium nucleatum, given the dual challenges of gene inactivation and the inherent genetic resistance of many strains? Traditional methods have been cumbersome and often inadequate. Addressing this, our work introduces a novel inducible CRISPR interference (CRISPRi) system in which dCas9 expression is controlled at the translation level by a theophylline-responsive riboswitch unit, and single-guide RNA expression is driven by the robust, constitutive rpsJ promo...
Source: Applied and Environmental Microbiology - January 8, 2024 Category: Microbiology Authors: Peng Zhou Bibek G C Flynn Stolte Chenggang Wu Source Type: research
Use of CRISPR interference for efficient and rapid gene inactivation in < em > Fusobacterium nucleatum < /em >
Appl Environ Microbiol. 2024 Jan 8:e0166523. doi: 10.1128/aem.01665-23. Online ahead of print.ABSTRACTHow can we effectively investigate the gene functions in Fusobacterium nucleatum, given the dual challenges of gene inactivation and the inherent genetic resistance of many strains? Traditional methods have been cumbersome and often inadequate. Addressing this, our work introduces a novel inducible CRISPR interference (CRISPRi) system in which dCas9 expression is controlled at the translation level by a theophylline-responsive riboswitch unit, and single-guide RNA expression is driven by the robust, constitutive rpsJ promo...
Source: Applied and Environmental Microbiology - January 8, 2024 Category: Microbiology Authors: Peng Zhou Bibek G C Flynn Stolte Chenggang Wu Source Type: research
Potassium sodium hydrogen citrate intervention on gut microbiota and clinical features in uric acid stone patients
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-15. doi: 10.1007/s00253-023-12953-y. Epub 2024 Jan 6.ABSTRACTThe high recurrence rate of renal uric acid stone (UAS) poses a significant challenge for urologists, and potassium sodium hydrogen citrate (PSHC) has been proven to be an effective oral dissolution drug. However, no studies have investigated the impact of PSHC on gut microbiota and its metabolites during stone dissolution therapy. We prospectively recruited 37 UAS patients and 40 healthy subjects, of which 12 patients completed a 3-month pharmacological intervention. Fasting vein blood was extracted and mid-stream uri...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Cheng Cao Feng Li Qi Ding Xiaohua Jin Wenjian Tu Hailiang Zhu Mubin Sun Jin Zhu Dongrong Yang Bo Fan Source Type: research
Effects of fecal microbial transplantation on police performance and transportation stress in Kunming police dogs
This study aimed to investigate the effects of FMT on performance and gut microflora in Kunming police dogs. Twenty Wolf Cyan dogs were randomly assigned to receive physiological saline or fecal suspension at low, medium, or high doses through oral gavage for 14 days. Growth performance, police performance, serum biochemical profiling, and gut microflora were determined 2-week post-FMT. Dogs after FMT treatment were also subjected to an hour road transportation and then were evaluated for serum stress indicators. Overall, FMT enhanced the growth performance and alleviated diarrhea rate in Kunming dogs with the greatest eff...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Qiu-Ye Lin Jin-Jing Du Hu Xu Ming-Kui Lv Le Xu Jie Li Zhen-Hui Cao Source Type: research
Potassium sodium hydrogen citrate intervention on gut microbiota and clinical features in uric acid stone patients
Appl Microbiol Biotechnol. 2024 Dec;108(1):1-15. doi: 10.1007/s00253-023-12953-y. Epub 2024 Jan 6.ABSTRACTThe high recurrence rate of renal uric acid stone (UAS) poses a significant challenge for urologists, and potassium sodium hydrogen citrate (PSHC) has been proven to be an effective oral dissolution drug. However, no studies have investigated the impact of PSHC on gut microbiota and its metabolites during stone dissolution therapy. We prospectively recruited 37 UAS patients and 40 healthy subjects, of which 12 patients completed a 3-month pharmacological intervention. Fasting vein blood was extracted and mid-stream uri...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Cheng Cao Feng Li Qi Ding Xiaohua Jin Wenjian Tu Hailiang Zhu Mubin Sun Jin Zhu Dongrong Yang Bo Fan Source Type: research
Effects of fecal microbial transplantation on police performance and transportation stress in Kunming police dogs
This study aimed to investigate the effects of FMT on performance and gut microflora in Kunming police dogs. Twenty Wolf Cyan dogs were randomly assigned to receive physiological saline or fecal suspension at low, medium, or high doses through oral gavage for 14 days. Growth performance, police performance, serum biochemical profiling, and gut microflora were determined 2-week post-FMT. Dogs after FMT treatment were also subjected to an hour road transportation and then were evaluated for serum stress indicators. Overall, FMT enhanced the growth performance and alleviated diarrhea rate in Kunming dogs with the greatest eff...
Source: Applied Microbiology and Biotechnology - January 6, 2024 Category: Microbiology Authors: Qiu-Ye Lin Jin-Jing Du Hu Xu Ming-Kui Lv Le Xu Jie Li Zhen-Hui Cao Source Type: research
Fusobacterium/Peptostreptococcus - A Case Report of Communityacquired Empyema Resulting in Surgical Decortication with Prolonged Antibiotic Therapy: A Case Series and Review of the Literature
CONCLUSION: This case highlights an unusual presentation of community-acquired empyema secondary to anaerobes without any risk factors for aspiration pneumonia. Therefore, clinicians should consider the possibility of anaerobic coverage in the treatment of community-acquired empyema in the appropriate setting.PMID:38178665 | DOI:10.2174/0118715265255809231107100450 (Source: Infectious Disorders Drug Targets)
Source: Infectious Disorders Drug Targets - January 5, 2024 Category: Infectious Diseases Authors: Garrastegui-Mercado Emmanuel Awad Chady Suresh Antony Source Type: research
Fusobacterium/Peptostreptococcus - A Case Report of Communityacquired Empyema Resulting in Surgical Decortication with Prolonged Antibiotic Therapy: A Case Series and Review of the Literature
CONCLUSION: This case highlights an unusual presentation of community-acquired empyema secondary to anaerobes without any risk factors for aspiration pneumonia. Therefore, clinicians should consider the possibility of anaerobic coverage in the treatment of community-acquired empyema in the appropriate setting.PMID:38178665 | DOI:10.2174/0118715265255809231107100450 (Source: Infectious Disorders Drug Targets)
Source: Infectious Disorders Drug Targets - January 5, 2024 Category: Infectious Diseases Authors: Garrastegui-Mercado Emmanuel Awad Chady Suresh Antony Source Type: research
Fusobacterium/Peptostreptococcus - A Case Report of Communityacquired Empyema Resulting in Surgical Decortication with Prolonged Antibiotic Therapy: A Case Series and Review of the Literature
CONCLUSION: This case highlights an unusual presentation of community-acquired empyema secondary to anaerobes without any risk factors for aspiration pneumonia. Therefore, clinicians should consider the possibility of anaerobic coverage in the treatment of community-acquired empyema in the appropriate setting.PMID:38178665 | DOI:10.2174/0118715265255809231107100450 (Source: Infectious Disorders Drug Targets)
Source: Infectious Disorders Drug Targets - January 5, 2024 Category: Infectious Diseases Authors: Garrastegui-Mercado Emmanuel Awad Chady Suresh Antony Source Type: research
Fusobacterium/Peptostreptococcus - A Case Report of Communityacquired Empyema Resulting in Surgical Decortication with Prolonged Antibiotic Therapy: A Case Series and Review of the Literature
CONCLUSION: This case highlights an unusual presentation of community-acquired empyema secondary to anaerobes without any risk factors for aspiration pneumonia. Therefore, clinicians should consider the possibility of anaerobic coverage in the treatment of community-acquired empyema in the appropriate setting.PMID:38178665 | DOI:10.2174/0118715265255809231107100450 (Source: Infectious Disorders Drug Targets)
Source: Infectious Disorders Drug Targets - January 5, 2024 Category: Infectious Diseases Authors: Garrastegui-Mercado Emmanuel Awad Chady Suresh Antony Source Type: research
Evaluation of the antibacterial effect of Epigallocatechin gallate on the major pathogens of canine periodontal disease and therapeutic effects on periodontal disease mice
ConclusionOur research demonstrates the significant antibacterial effects of EGCG against the prevalent bacterium P. gulae in canine PD. Moreover, EGCG exhibits anti-inflammatory properties and proves effective in addressing bone loss in a PD mouse model. These findings collectively suggest the therapeutic potential of EGCG in the treatment of canine PD. The outcomes of this study contribute valuable data, laying the foundation for further exploration and screening of alternative antibiotic drugs to advance the management of canine PD. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - January 5, 2024 Category: Microbiology Source Type: research
Digestive characteristics of extracellular polysaccharide from Lactiplantibacillus plantarum T1 and its regulation of intestinal microbiota
This study assessed the potential prebiotic characteristics of the previously reported Lactiplantibacillus plantarum extracellular polysaccharide (EPS-T1) with immunological activity. EPS-T1 was a novel heteropolysaccharide composed of glucose and galactose (1.00:1.21), with a molecular weight of 1.41 × 106 Da. The monosaccharide composition, molecular weight, fourier transform infrared, and 1H NMR analysis showed that EPS-T1 was well tolerated in the simulated oral cavity, gastric fluid, and small intestinal fluid environments, and was not easily degraded. Meanwhile, EPS-T1 could effectively be used as a carbon source to...
Source: International Journal of Biological Macromolecules - January 4, 2024 Category: Biochemistry Authors: Xueliang Zhang Changliang Zhang Luyao Xiao Xiaogan Zhao Kai Ma Feng Ji Elham Azarpazhooh Marjan Ajami Xin Rui Wei Li Source Type: research
The ways Fusobacterium nucleatum translocate to breast tissue and contribute to breast cancer development
AbstractBreast cancer is among the most prevalent malignancies in women worldwide. Epidemiological findings suggested that periodontal diseases may be associated with breast cancer, among whichFusobacterium nucleatum is considered an important cross-participant. In this work, we comprehensively summarize the known mechanisms of howF. nucleatum translocates to, colonizes in mammary tumors, and promotes the carcinogenesis. Specifically,F. nucleatum translocates to mammary tissue through the mammary –intestinal axis, direct nipple contact, and hematogenous transmission. Subsequently,F. nucleatum takes advantage of fusobacte...
Source: Molecular Oral Microbiology - January 4, 2024 Category: Microbiology Authors: Xinyu Guo,
Ke Yu,
Ruijie Huang Tags: REVIEW ARTICLE Source Type: research
GSE243383 Phagocytes as Trojan horse for Fusobacterium nucleatum routing to the tumor tissues and enhance tumor metastasis
Contributors : Fangfang Chen ; Songhe Guo ; Yiqiu Li ; Yongfan Lu ; Jinzhu Mao ; Le Liu ; Shengxin ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe facultative intracellular bacterium Fusobacterium nucleatum (Fn), mediates tumorigenesis and progression in CRC. However, the origin of intracellular Fn and the role of Fn-infected phagocytes in tumor microenvironment remains unclear. Here, we observed Fn-infected neutrophils/macrophages (PMNs/M Φ) is accumulated in CRC tumor tissues. Fn can survive inside of PMNs by reducing intracellular ROS levels. The lysozyme inhibitor ...
Source: GEO: Gene Expression Omnibus - January 1, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Does a Dysbiotic Oral Microbiome Trigger the Risk of Chronic Inflammatory Disease?
AbstractPurpose of ReviewThis paper studies the different mechanisms that link oral dysbiosis and various inflammatory diseases.Recent FindingsOral bacteria have been found in atherosclerotic plaques. Experiments in mouse models have shown that after inoculation withPorphyromonas gingivalis, there is a higher brain barrier permeability.Fusobacterium nucleatum is present in higher amounts in patients with colorectal cancer than in healthy subjects.Porphyromonas gingivalis, along with other oral bacteria, has a strong capacity for peptide citrullination, which increases the risk of developing rheumatoid arthritis.SummaryDiff...
Source: Current Treatment Options in Allergy - December 27, 2023 Category: Allergy & Immunology Source Type: research
