Platelet plug formation in whole blood is enhanced in the presence of Porphyromonas gingivalis
SummaryPorphyromonas gingivalis is a gram ‐negative anaerobic bacterium and an etiologic agent of adult periodontitis. By inducing a dysbiotic state within the host microbiota it contributes to a chronic inflammatory environment in the oral cavity. Under some circumstances, the oral bacteria may gain access to systemic circulation. While the most widely recognized function of platelets is to reduce hemorrhage in case of vascular damage, it is known that platelets are also involved in the hematologic responses to bacterial infections. Some pathogenic bacteria can interact with platelets, triggering their activation and ag...
Source: Molecular Oral Microbiology - September 18, 2020 Category: Microbiology Authors: William A. Chen, Hansel M. Fletcher, Joseph Gheorghe, Udochukwu Oyoyo, Danilo S. Boskovic Tags: ORIGINAL ARTICLE Source Type: research

The enterococcal PASTA kinase: a sentinel for cell envelope stress
AbstractEnterococci are Gram ‐positive, opportunistic pathogens that reside throughout the gastrointestinal tracts of most terrestrial organisms. Enterococci are resistant to many antibiotics, which makes enterococcal infections difficult to treat. Enterococci are also particularly hardy bacteria that can tolerate a variety o f environmental stressors. Understanding how enterococci sense and respond to the extracellular environment to enact adaptive biological responses may identify new targets that can be exploited for development of treatments for enterococcal infections. Bacterial eukaryotic‐like serine/threonine ki...
Source: Molecular Oral Microbiology - September 18, 2020 Category: Microbiology Authors: Du šanka Djorić, Nicole E. Minton, Christopher J. Kristich Tags: INVITED REVIEW Source Type: research

Comprehensive profiling of protein lysine acetylation and its overlap with lysine succinylation in the Porphyromonas gingivalis fimbriated strain ATCC 33277
This study provides a significant beginning for further investigating the role of Kac and Ksuc in the pathogenicity ofP. gingivalis. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - September 16, 2020 Category: Microbiology Authors: Jumei Zeng, Leng Wu, Qiushi Chen, Lingyun Wang, Wei Qiu, Xin Zheng, Xiaoming Yin, Jie Liu, Yan Ren, Yuqing Li Tags: ORIGINAL ARTICLE Source Type: research

Role of the RprY Response Regulator in P. gingivalis Community Development and Virulence
In this study we examined cross phosphorylation of RprY on tyrosine residues and its importance for RprY function. We show that RprY reacts with phosphotyrosine antibodies, and a tyrosine (Y) resi due at position 41 is predicted to be solvent accessible. Loss of RprY increased the level of heterotypic community development withStreptococcus gordonii, and the community ‐suppressive function of RprY required Y41. Expression of the Mfa1 fimbrial adhesin was increased in therprY mutant and in the mutant complemented withrprY containing a Y41F mutation. In a microscale thermophoresis assay, recombinant RprY protein bound to t...
Source: Molecular Oral Microbiology - September 16, 2020 Category: Microbiology Authors: Daonan Shen, John D. Perpich, Kendall S. Stocke, Lan Yakoumatos, Zackary R. Fitzsimonds, Chengcheng Liu, Daniel P. Miller, Richard J. Lamont Tags: ORIGINAL ARTICLE Source Type: research

Inflammatory response of uric acid produced by Porphyromonas gingivalis gingipains
In this study, we demonstrate thatP. gingivalis gingipains play a role in THP ‐1 macrophage uric acid production by increasing the expression and activity of xanthine oxidoreductase. Uric acid sodium salt induces caspase‐1 activation, cell death, and the expression of proinflammatory cytokines, including IL‐1α, IL‐6, and IL‐8, in the human keratinocyte HOK‐16B ce ll line. Our results suggest that gingipain‐induced uric acid can mediate inflammation in periodontal tissue cells. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - August 14, 2020 Category: Microbiology Authors: Hye ‐Kyung Jun, Sun‐Jin An, Hyun Young Kim, Bong‐Kyu Choi Tags: ORIGINAL ARTICLE Source Type: research

Deletion of csn2 gene affects acid tolerance and exopolysaccharide synthesis in Streptococcus mutans
In this study, we investigated whethercsn2 deletion would affectS. mutans physiology and virulence gene expression. We used microscopic imaging, acid killing assays, pH drop, biofilm formation and exopolysaccharide (EPS) prodction tests to determine whethercsn2 deletion influencedS. mutans colony morphology, acid tolerance/production and glucan formation abilities. Comparisons were made between qRT ‐PCR data from the UA159 andcsn2 deletion strain to determine the impact ofcsn2 knockout onS. mutans gene expression. The results showed that deletion ofS. mutans csn2 changed its colony morphotype and made it more sensitive t...
Source: Molecular Oral Microbiology - August 14, 2020 Category: Microbiology Authors: Anqi Zhang, Jiamin Chen, Tao Gong, Miao Lu, Boyu Tang, Xuedong Zhou, Yuqing Li Tags: ORIGINAL ARTICLE Source Type: research

Filifactor alocis ‐derived extracellular vesicles inhibit osteogenesis through TLR2 signaling
AbstractFilifactor alocis, an asaccharolytic anaerobic Gram ‐positive rod (AAGPR), is an emerging marker of periodontitis. Severe periodontitis causes destruction of the alveolar bone that supports teeth and can even lead to tooth loss. Based on our previous report thatF. alocis‐derived extracellular vesicles (FA EVs) contain various effector molecules and have immunostimulatory activity, we investigated the effect of FA EVs on osteogenesis using mouse bone‐derived mesenchymal stromal cells (BMSCs). FA EVs dramatically inhibited bone mineralization similarly to whole b acteria and reduced the expression levels of ost...
Source: Molecular Oral Microbiology - July 23, 2020 Category: Microbiology Authors: Min ‐Kyoung Song, Hyun Young Kim, Bong‐Kyu Choi, Hong‐Hee Kim Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 35, Issue 4, Page i-iii, August 2020. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - July 20, 2020 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

In vivo and ex vivo actions of a novel P. gingivalis inhibitor on multi ‐species biofilm, inflammatory response and periodontal bone loss
SummaryChronic periodontitis is one of the most common infectious inflammatory diseases worldwide. Current therapeutic options for the disease are only partially and temporarily successful due to periodontal re ‐emergence of pathogens such asPorphyromonas gingivalis, a keystone bacterium in the oral microbial communities, which elicits a dysbiosis between the microbiota and the host. Previously, we reported a peptide inhibitor ofP. gingivalis (SAPP) that specifically targetsP. gingivalis and reduces its virulence potentialin vitro. Here, we show that SAPP can modulate the ability ofP. gingivalis to suppress the host inna...
Source: Molecular Oral Microbiology - June 30, 2020 Category: Microbiology Authors: Meng ‐Hsuan Ho, Hatice Hasturk, Daphne F. Young, Hua Xie Tags: ORIGINAL ARTICLE Source Type: research

Sequence and characterization of shuttle vectors for molecular cloning in Porphyromonas, Bacteroides, and related bacteria
AbstractThere is a lack of shuttle vectors to be needed for investigations into the genetics ofPorphyromonas gingivalis and related species. To better understand the prevalence of candidates for such tools we have examined multiple strains of black pigmented anaerobes (clinical and laboratory isolates) for plasmids. As no plasmids were found inP. gingivalis strains, we have used the pYH420 plasmid, derived fromP. asaccharolytica, as backbone to construct a shuttle vector in combination with pUC19 fromEscherichia coli. Nucleotide sequence determination of the pYH420 plasmid revealed that that it contained a gene with simila...
Source: Molecular Oral Microbiology - June 26, 2020 Category: Microbiology Authors: Kevin R. Jones, B. Ross Belvin, Francis L. Macrina, Janina P. Lewis Tags: ORIGINAL ARTICLE Source Type: research

Proteomic analysis of Fusobacterium nucleatum growth in biofilm versus planktonic state
SummaryFusobacterium nucleatum is isolated from both supra ‐ and sub‐gingival dental biofilms in humans and has been implicated in the aetiology of periodontitis. Also, this bacterium plays an important role in serious infections in other parts of the body. The aim of this investigation was to study the protein differential expression ofF. nucleatum when growing on biofilm, compared to planktonic state, using proteomic analysis by the 2D ‐DIGE™ system. Sixty‐eight proteins were differentially expressed during biofilm growth (1.5‐fold, p
Source: Molecular Oral Microbiology - June 18, 2020 Category: Microbiology Authors: Arancha Llama ‐Palacios, Oksana Potupa, María C. Sánchez, Elena Figuero, David Herrera, Mariano Sanz Tags: ORIGINAL ARTICLE Source Type: research

Effects of Porphyromonas gingivalis and Fusobacterium nucleatum on inflammasomes and their regulators in H400 cells
ConclusionPg alone increased IL ‐1β by upregulating AIM2, NLRP3 and downregulating POP1.Fn promoted IL ‐1β by increasing AIM2 and downregulating POP1.Pg+ATP with or withoutFn upregulated NLRP3, IL ‐1β by downregulating POP1. Periodontal pathogens may contribute to HNSCC pathogenesis by increasing the IL‐1β response due to inflammasome dysregulation. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - June 9, 2020 Category: Microbiology Authors: K übra Aral, Michael R Milward, Dhanak Gupta, Paul R Cooper Tags: ORIGINAL ARTICLE Source Type: research

Aggregatibacter actinomycetemcomitans leukotoxin: From mechanism to targeted anti ‐toxin therapeutics
Aggregatibacter actinomycetemcomitans is a Gram ‐negative bacterium associated with localized aggressive periodontitis, as well as other systemic diseases. Several studies have demonstrated that clinical isolates from diseased patients frequently belong to specific clones ofA. actinomycetemcomitans that produce significantly higher amounts of leukotoxin (LtxA) suggesting that LtxA might play a key role in pathogenicity. In this article, we review the mechanisms by which LtxA interacts with and kills host cells, highlight the remaining open questions, and demonstrate how knowledge of these mechanisms, enable the design of...
Source: Molecular Oral Microbiology - May 5, 2020 Category: Microbiology Authors: Eric Krueger, Angela C. Brown Tags: REVIEW ARTICLE Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 35, Issue 3, Page i-iv, June 2020. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - May 5, 2020 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

Streptococcus mutans SpxA2 relays the signal of cell envelope stress from LiaR to effectors that maintain cell wall and membrane homeostasis
In this study, the role of thespxA2 transcriptional regulator in these two pathways, and overall cell envelope homeostasis, was examined. Loss ofspxA2 resulted in an increase in the proportion of saturated fatty acids in theS. mutans membrane and altered transcription of several genes involved in the production of these membrane fatty acids, includingfabT andfabM. Furthermore, activity of the F1F0‐ATPase was increased in the ∆spxA2 strain. Transcription ofspxA2 was elevated in the presence of a variety of membrane stressors, and highly dependent on theliaR component of the LiaFSR system, which is known to sense cell en...
Source: Molecular Oral Microbiology - May 5, 2020 Category: Microbiology Authors: Jonathon L. Baker, Sarah Saputo, Roberta C. Faustoferri, Robert G. Quivey Tags: ORIGINAL ARTICLE Source Type: research

The S. mutans mntE gene encodes a manganese efflux transporter
In conclusion, theS. mutans SMU_1176 gene, which we renamedmntE, is a manganese efflux transporter that contributes to essential metal ion homeostasis as part of the SloR regulon. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - May 5, 2020 Category: Microbiology Authors: Joseph O'Brien, Alexander Pastora, Andrew Stoner, Grace Spatafora Tags: ORIGINAL ARTICLE Source Type: research

Ribosomal protein L4 of Lactobacillus rhamnosus LRB alters resistance to macrolides and other antibiotics
In this study, we isolated a nontargeted mutant that was particularly sensitive to acid stress. Using next generation sequencing, we further mapped the putative mutations in the genome and found that the mutant had acquired a deletion of 75 base pairs in therplD gene that encodes the large ribosomal subunit L4. The mutant had a growth defect at 37 °C and at ambient temperature. Further antibiotic sensitivity analyses indicated that the mutant is relatively more resistant to erythromycin and chloramphenicol; two antibiotics that target the 50S subunit. In contrast, the mutant was more sensitive to tetracycline, which ta...
Source: Molecular Oral Microbiology - May 5, 2020 Category: Microbiology Authors: Saswati Biswas, Andrew Keightley, Indranil Biswas Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 35, Issue 3, Page i-iv, June 2020. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - May 5, 2020 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

Saliva is a non ‐negligible factor in the spread of COVID‐19
SummarySARS ‐CoV‐2, a novel emerging coronavirus, has caused severe disease (COVID‐19), and rapidly spread worldwide since the beginning of 2020. SARS‐CoV‐2 mainly spreads by coughing, sneezing, droplet inhalation, and contact. SARS‐CoV‐2 has been detected in saliva samples, making saliva a potent ial transmission route for COVID‐19. The participants in dental practice confront a particular risk of SARS‐CoV‐2 infection due to close contact with the patients and potential exposure to saliva‐contaminated droplets and aerosols generated during dental procedures. In addition, saliva‐con taminated surfac...
Source: Molecular Oral Microbiology - May 4, 2020 Category: Microbiology Authors: Yuqing Li, Biao Ren, Xian Peng, Tao Hu, Jiyao Li, Tao Gong, Boyu Tang, Xin Xu, Xuedong Zhou Tags: ORIGINAL ARTICLE Source Type: research

Lipoteichoic acid of Enterococcus faecalis interferes with Porphyromonas gingivalis lipopolysaccharide signaling via IRAK ‐M upregulation in human periodontal ligament cells
AbstractPeriodontitis is a chronic inflammatory disease of the gums caused by infection with multispecies oral bacteria. Since the periodontopathic bacteriaPorphyromonas gingivalis together withEnterococcus faecalis are frequently detected in patients with a severe form of periodontitis, interactions between their virulence factors might play an important role in progression of the disease.P. gingivalis andE. faecalis possess lipopolysaccharide (Pg.LPS) and lipoteichoic acid (Ef.LTA), respectively, as the major virulence factors inducing inflammatory responses. However, the combinatorial effect of these virulence factors o...
Source: Molecular Oral Microbiology - April 20, 2020 Category: Microbiology Authors: Jintaek Im, Jung Eun Baik, Dongwook Lee, Kee ‐Yeon Kum, Cheol‐Heui Yun, Ok‐Jin Park, Seung Hyun Han Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 35, Issue 2, Page i-iii, April 2020. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - March 7, 2020 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

The S. mutans mntE gene encodes a manganese efflux transporter
In conclusion, theS. mutans SMU_1176 gene, which we renamedmntE, is a manganese efflux transporter that contributes to essential metal ion homeostasis as part of the SloR regulon. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - March 4, 2020 Category: Microbiology Authors: Joseph O ’Brien, Alexander Pastora, Andrew Stoner, Grace Spatafora Tags: ORIGINAL ARTICLE Source Type: research

Aggregatibacter actinomycetemcomitans leukotoxin: From mechanism to targeted anti ‐toxin therapeutics
AbstractAggregatibacter actinomycetemcomitans is a Gram ‐negative bacterium associated with localized aggressive periodontitis, as well as other systemic diseases. This organism produces a number of virulence factors, all of which provide some advantage to the bacterium. Several studies have demonstrated that clinical isolates from diseased patients, p articularly those of African descent, frequently belong to specific clones ofA. actinomycetemcomitans that produce significantly higher amounts of a protein exotoxin belonging to the repeats ‐in‐toxin (RTX) family, leukotoxin (LtxA), while isolates from healthy patient...
Source: Molecular Oral Microbiology - February 15, 2020 Category: Microbiology Authors: Eric Krueger, Angela C. Brown Tags: REVIEW ARTICLE Source Type: research

CRISPR ‐Cas systems in oral microbiome: From immune defense to physiological regulation
CRISPR ‐Cas system, found in bacteria and archaea, provides sequence‐based adaptive immunity against mobile genetic elements, including phages and plasmids. The oral cavity contains approximately 700 prokaryote species harboring known CRISPR‐Cas systems. Here, we review the canonical and novel functi ons of the CRISPR‐Cas systems in oral microbiome, including defense against foreign mobile elements, biofilm formation, acquisition of resistance genes, DNA repair, regulation of interspecific competition and intraspecific diversification, stress responses, and gene expression regulation. AbstractThe clustered regularl...
Source: Molecular Oral Microbiology - February 14, 2020 Category: Microbiology Authors: Tao Gong, Jumei Zeng, Boyu Tang, Xuedong Zhou, Yuqing Li Tags: REVIEW ARTICLE Source Type: research

Streptococcus mutans SpxA2 relays the signal of cell envelope stress from LiaR to effectors that maintain cell wall and membrane homeostasis
In this study, the role of thespxA2 transcriptional regulator in these two pathways, and overall cell envelope homeostasis, was examined. Loss ofspxA2 resulted in an increase in the proportion of saturated fatty acids in theS. mutans membrane and altered transcription of several genes involved in the production of these membrane fatty acids, includingfabT andfabM. Furthermore, activity of the F1F0‐ATPase was increased in the ∆spxA2 strain. Transcription ofspxA2 was elevated in the presence of a variety of membrane stressors, and highly dependent on theliaR component of the LiaFSR system, which is known to sense cell en...
Source: Molecular Oral Microbiology - February 11, 2020 Category: Microbiology Authors: Jonathon L. Baker, Sarah Saputo, Roberta C. Faustoferri, Robert G. Quivey Tags: ORIGINAL ARTICLE Source Type: research

Quantitative proteomic analysis of the type IX secretion system mutants in Porphyromonas gingivalis
AbstractPorphyromonas gingivalis is an anaerobic, Gram ‐negative human oral pathogen highly associated with chronic periodontitis.P. gingivalis utilises the type IX secretion system (T9SS) to transport many of its virulence factors including the gingipains to the cell surface. The T9SS is comprised of at least 16 proteins and the involvement of these 16 proteins in the T9SS has been verified by creating gene deletion mutants inP. gingivalis. These T9SS mutants are regularly utilised to understand how these proteins function together to allow the secretion of the T9SS substrates. We performed label ‐free quantitative pr...
Source: Molecular Oral Microbiology - February 11, 2020 Category: Microbiology Authors: Dhana G. Gorasia, Michelle D. Glew, Paul D. Veith, Eric C. Reynolds Tags: ORIGINAL ARTICLE Source Type: research

Ribosomal Protein L4 of Lactobacillus rhamnosus LRB alters resistance to macrolides and other antibiotics
In this study, we isolated a nontargeted mutant that was particularly sensitive to acid stress. Using next generation sequencing, we further mapped the putative mutations in the genome and found that the mutant had acquired a deletion of 75 base pairs in therplD gene that encodes the large ribosomal subunit L4. The mutant had a growth defect at 37 °C and at ambient temperature. Further antibiotic sensitivity analyses indicated that the mutant is relatively more resistant to erythromycin and chloramphenicol; two antibiotics that target the 50S subunit. In contrast, the mutant was more sensitive to tetracycline, which ta...
Source: Molecular Oral Microbiology - February 5, 2020 Category: Microbiology Authors: Saswati Biswas, Andrew Keightley, Indranil Biswas Tags: ORIGINAL ARTICLE Source Type: research

CRISPR ‐Cas system in oral microbiome: from immune defense to physiological regulation
AbstractThe clustered regularly interspaced short palindromic repeats with CRISPR ‐associated proteins (CRISPR‐Cas) system, found in bacteria and archaea, provides sequence‐based adaptive immunity against mobile genetic elements, including phages and plasmids. The oral cavity contains approximately 700 prokaryote species harboring known CRISPR‐Cas systems, including type I, type II, type III, type V, and type VI, and unidentified CRISPR‐Cas systems. There is increasing evidence to suggest that different CRISPR‐Cas systems in the human oral microbiome can affect bacterial physiology through different mechanisms....
Source: Molecular Oral Microbiology - January 29, 2020 Category: Microbiology Authors: Tao Gong, Jumei Zeng, Boyu Tang, Xuedong Zhou, Yuqing Li Tags: REVIEW ARTICLE Source Type: research

Identification of functional domains of the minor fimbrial antigen involved in the interaction of Porphyromonas gingivalis with oral streptococci
AbstractPorphyromonas gingivalis is associated with chronic periodontitis and may initially colonize the oral cavity by adhering to streptococci. Adhesion to streptococci is driven by interaction of the minor fimbrial antigen (Mfa1) with streptococcal antigen I/II. We identified the region of antigen I/II required for this interaction and developed small molecule mimetics that inhibitedP. gingivalis adherence. However, the functional motifs of Mfa1 involved in the interaction with antigen I/II remain uncharacterized. A series of N ‐ and C‐terminal peptide fragments of Mfa1 were expressed and tested for inhibition ofP. ...
Source: Molecular Oral Microbiology - January 28, 2020 Category: Microbiology Authors: Mohammad Roky, John O. Trent, Donald R. Demuth Tags: ORIGINAL ARTICLE Source Type: research

Heterogeneity of Streptococcus anginosus ß‐hemolysis in relation to CRISPR/Cas
AbstractStreptococcus anginosus is a commensal of the oral mucosa that can cause severe invasive infections. A considerable proportion ofStreptococcus anginosus strains are ß‐hemolytic due to the presence of an SLS‐like gene cluster. However, the majority of strains do not display ß‐hemolysis. To investigate ß‐hemolysin heterogeneity inS. anginosus, we determined the presence ofsag genes and correlated it with the presence of CRISPR/Cas genes in a collection of ß‐hemolytic and non‐ß‐hemolytic strains. All of the ß‐hemolytic strains carried thesag gene cluster. In contrast...
Source: Molecular Oral Microbiology - January 24, 2020 Category: Microbiology Authors: Richard Bauer, Nathalie Neffgen, Aline Grempels, Martina Furitsch, Stefanie Mauerer, Salome Barbaqadze, Gerhard Haase, Hans Kestler, Barbara Spellerberg Tags: ORIGINAL ARTICLE Source Type: research

Nanoscale Adhesion Forces of glucosyltransferase B and C genes regulated streptococcal mutans probed by AFM
AbstractGlucosyltransferases (Gtfs), represented by GtfB and GtfC, are important virulence factors ofStreptococcus mutans and the major etiologic pathogens of tooth decay. However, the individual roles ofgtfB andgtfC in the initial attachment ofS. mutan are not known. We used atomic force microscopy to explore the contribution ofgtfB andgtfC, as well as enamel ‐surface roughness, on the initial attachment ofS. mutans. Adhesion forces of fourS. mutans strains (wild ‐type, ΔgtfB, ΔgtfC and ΔgtfBC), onto etched enamel surfaces, were determined.Force curves showed that, with increasing etching time from 0...
Source: Molecular Oral Microbiology - January 20, 2020 Category: Microbiology Authors: Rui Wang, Ling Deng, Dong Chen, Peiyao Wu, Liying Hao, Yigan Wang, Tianjiao Li, Li Jiang Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 35, Issue 1, Page i-iii, January 2020. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - January 13, 2020 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

Epigallocatechin gallate inhibits leukotoxin release by Aggregatibacter actinomycetemcomitans by promoting association with the bacterial membrane
AbstractThe oral pathogen,Aggregatibacter actinomycetemcomitans, produces a number of virulence factors, including a leukotoxin (LtxA), which specifically kills human white blood cells, to provide a colonization advantage to the bacterium. Strains ofA. actinomycetemcomitans that produce more LtxA have been more closely linked to disease, indicating that this toxin plays a key role in pathogenesis of the bacterium. Disruption of the activity of LtxA thus represents a promising approach to reducing the pathogenicity of the bacterium. Catechins are polyphenolic molecules derived from plants, which have shown potent antibacter...
Source: Molecular Oral Microbiology - December 9, 2019 Category: Microbiology Authors: En Hyung Chang, Peter Giaquinto, Joanne Huang, Nataliya V. Balashova, Angela C. Brown Tags: ORIGINAL ARTICLE Source Type: research

Periodontal pathogens and clinical parameters in chronic periodontitis
ConclusionIn this specific population, we found that the best model in predicting the mean pocket depth was microbial dybiosis using the dysbiosis ratio taxa formula. While further studies are needed to assess the validity of these results on the general population, such a dysbiosis ratio could be used in the future to monitor the subgingival microbiota. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - November 29, 2019 Category: Microbiology Authors: Emile Boyer, B énédicte Martin, Sandrine Le Gall‐David, Shao Bing Fong, Yves Deugnier, Martine Bonnaure‐Mallet, Vincent Meuric Tags: ORIGINAL ARTICLE Source Type: research

Porphyromonas gingivalis genes conferring fitness in a tobacco ‐rich environment
This study identifies, for the first time, a subset ofP. gingivalis genes required for surviving the plethora of insults present in cigarette smoke. Such conditionally essential genes may delineate bacterial persistence strategies and represent novel therapeutic foci for the prevention ofP. gingivalis infection and related diseases in smokers and in general. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - November 19, 2019 Category: Microbiology Authors: Justin A. Hutcherson, Himabindu Gogenini, Gwyneth J. Lamont, Daniel P. Miller, Zuzanna Nowakowska, Anna M. Lasica, Chengcheng Liu, Jan Potempa, Richard J. Lamont, Deborah Yoder ‐Himes, David A. Scott Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 34, Issue 6, Page i-iii, December 2019. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - November 12, 2019 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis
In this study, we purified and characterized the protein profiles of EVs fromF. alocis and investigated their immunostimulatory activity on human monocytic THP ‐1 and human oral keratinocyte HOK‐16B cell lines. Highly pure EVs were obtained fromF. alocis using density gradient ultracentrifugation. Nanoparticle tracking analysis and transmission electron microscopy showed thatF. alocis EVs were between 50 nm and 270 nm in diameter. Proteome analysis identified 28 proteins, including lipoproteins, autolysins,F. alocis complement inhibitor (FACIN), transporter ‐related proteins, metabolism‐related proteins, and riboso...
Source: Molecular Oral Microbiology - November 1, 2019 Category: Microbiology Authors: Hyun Young Kim, Younggap Lim, Sun ‐Jin An, Bong‐Kyu Choi Tags: ORIGINAL ARTICLE Source Type: research

Tribute: Edward ‘Ned’ Lally
Molecular Oral Microbiology, EarlyView. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - October 21, 2019 Category: Microbiology Authors: Bruce J. Shenker, Kathleen Boesze ‐Battaglia, Caroline A. Genco, Ann Progulske‐Fox Tags: Tribute Source Type: research

Streptococcal peptides that signal Enterococcus faecalis cells carrying the pheromone ‐responsive conjugative plasmid pAM373
AbstractPheromone ‐mediated conjugative transfer of enterococcal plasmids can contribute to the dissemination of genes involved in antibiotic resistance, fitness, and virulence among co‐residents of mixed microbial communities. We have previously shown that intergeneric signaling by theStreptococcus gordonii strain Challis heptapeptide s.g.cAM373 (SVFILAA) induces an aggregation ‐substance‐mediated mating response and facilitates plasmid transfer fromEnterococcus faecalis cells carrying the pheromone ‐responsive plasmid pAM373 to both pheromone producing and non‐pheromone producing oral streptococcal recipients...
Source: Molecular Oral Microbiology - October 14, 2019 Category: Microbiology Authors: M. Margaret Vickerman, Jillian M. Mansfield Tags: ORIGINAL ARTICLE Source Type: research

Type I Interferon and Interferon ‐stimulated Gene Expression in Oral Epithelial Cells
AbstractOral epithelial cells (OEC) represent the first site of host interaction with viruses that infect the body through the oral route, however their innate antiviral defense mechanisms have yet to be defined. Previous studies have determined that OEC express pathogen ‐, damage‐ or danger‐associated molecular patterns (PAMPs, or DAMPs), but their expression of key antiviral innate immune mediators, including type I interferons (type I IFN) and interferon‐stimulated genes (ISGs) has not been studied extensively. We used the oral keratinocyte cell line, OKF 6/TERT1 in the presence and absence of the viral mimics p...
Source: Molecular Oral Microbiology - September 13, 2019 Category: Microbiology Authors: David C. Brice, Erika Figgins, Fahong Yu, Gill Diamond Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 34, Issue 5, Page i-iv, October 2019. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - September 12, 2019 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

Type IX secretion system is pivotal for expression of gingipain ‐associated virulence of Porphyromonas gingivalis
This article is protected by copyright. All rights reserved. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - August 20, 2019 Category: Microbiology Authors: Malgorzata Benedyk, Agata Marczyk, Barbara Chru ścicka Tags: Original Article Source Type: research

Peptide and non ‐peptide mimetics as potential therapeutics targeting oral bacteria and oral biofilms
This article is protected by copyright. All rights reserved. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - August 7, 2019 Category: Microbiology Authors: Maryta N. Sztukowska, Mohammad Roky, Donald R. Demuth Tags: Invited Review Source Type: research

Sortase A ‐mediated modification of the Streptococcus mutans transcriptome and virulence traits
This article is protected by copyright. All rights reserved. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - July 25, 2019 Category: Microbiology Authors: Xuan Chen, Yuanli He, Haixia Liu, Ying Song, Kaixin Xiong, Xian Peng, Chengcheng Liu, Ling Zou Tags: Original Article Source Type: research

Regulation of IL ‐24 in human oral keratinocytes stimulated with Tannerella forsythia
This article is protected by copyright. All rights reserved. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - July 22, 2019 Category: Microbiology Authors: Yeon ‐Kyeong Ko, Sun‐Jin An, Na‐Young Han, Hookeun Lee, Bong‐Kyu Choi Tags: Original Article Source Type: research

Issue Information
Molecular Oral Microbiology, Volume 34, Issue 4, Page i-iv, August 2019. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - July 11, 2019 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research

EzrA, a cell shape regulator contributing to biofilm formation and competitiveness in Streptococcus mutans
This article is protected by copyright. All rights reserved. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - July 9, 2019 Category: Microbiology Authors: Zhenting Xiang, Zongbo Li, Zhi Ren, Jumei Zeng, Xian Peng, Yuqing Li, Jiyao Li Tags: ORIGINAL ARTICLE Source Type: research

Differential responses of endothelial cells on three ‐dimensional scaffolds to lipopolysaccharides from periodontopathogens
This article is protected by copyright. All rights reserved. (Source: Molecular Oral Microbiology)
Source: Molecular Oral Microbiology - July 4, 2019 Category: Microbiology Authors: Diego Fernando Gualtero, Gloria Ines Lafaurie, Marta Raquel Fontanilla Tags: Original Article Source Type: research