Burkholderia Cepacia Research This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Evaluation of lipase access tunnels and analysis of substance transport in comparison with experimental data
Bioprocess Biosyst Eng. 2022 May 18. doi: 10.1007/s00449-022-02731-x. Online ahead of print.ABSTRACTLipases (E.C. 3.1.1.3) have buried active sites and used access tunnels in the transport of substrates and products for biotransformation processes. Computational methods are used to predict the trajectory and energy profile of ligands through these tunnels, and they complement the experimental methodologies because they filter data, optimizing laboratory time and experimental costs. Access tunnels of Burkholderia cepacia lipase (BCL), Candida rugosa lipase (CRL), and porcine pancreas lipase (PPL) and the transport of fatty ...
Source: Bioprocess and Biosystems Engineering - May 18, 2022 Category: Biomedical Engineering Authors: J éssica Jéssi C de Melo Jesica Ribeiro Gon çalves Luma M de S Brand ão Ranyere L Souza Matheus M Pereira Álvaro S Lima Cleide M F Soares Source Type: research

FE-SEM/EDX Based Zinc Mobilization Analysis of Burkholderia cepacia and Pantoea rodasii and Their Functional Annotation in Crop Productivity, Soil Quality, and Zinc Biofortification of Paddy
The experimental study was contrived to characterize two zinc-solubilizing bacteria (ZSB), namely BMRR126 and BMAR64, and their role in zinc (Zn) biofortification of rice. These bacteria solubilized Zn profoundly, determined qualitatively by halo-zone formation on a solid medium and quantitatively in a liquid broth by AAS and SEM-EDX. The lowering of pH and contact angle assessment of the liquid broth unveiled the establishment of the acidic conditions in a medium suitable for Zn solubilization. The characterization of both isolates on the basis of 16S rRNA gene analysis was identified as Burkholderia cepacia and Pantoea r...
Source: Frontiers in Microbiology - May 6, 2022 Category: Microbiology Source Type: research

Susceptibility of Burkholderia cepacia Complex to Ceftazidime/Avibactam and Standard Drugs of Treatment for Cystic Fibrosis Patients
Microbial Drug Resistance, Ahead of Print. (Source: Microbial Drug Resistance)
Source: Microbial Drug Resistance - May 5, 2022 Category: Microbiology Authors: Frieder Schaumburg Evgeny A. Idelevich Alexander Mellmann Barbara C. Kahl Source Type: research

Biocatalytic asymmetric synthesis of secondary allylic alcohols using Burkholderia cepacia lipase immobilized on multiwalled carbon nanotubes
Chirality. 2022 May 4. doi: 10.1002/chir.23454. Online ahead of print.ABSTRACTThe lipase from Burkholderia cepacia (BCL) was immobilized through physical adsorption on pristine and functionalized multiwalled carbon nanotubes (MWCNTs) with carboxyl or amine groups and used in the stereoselective acylation of (R,S)-1-octen-3-ol (1) and (R,S)-(E)-4-phenyl-3-buten-2-ol (4) with vinyl acetate. All immobilized preparations produced better results than free BCL. For (R,S)-4, 50% conversion and E > 200 were obtained in n-hexane or in solvent-free medium. For (R,S)-1, in solvent-free medium, the conversion was 38% with a slight ...
Source: Chirality - May 4, 2022 Category: Molecular Biology Authors: Michele do Rocio Gon çalves Dias Gesieli Prado Cardoso da Silva Alysson de Pauloveloso Nadia Krieger Cristiane Piliss ão Source Type: research

Viruses, Vol. 14, Pages 938: The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1
Dennis Bacteriophage JC1 is a Podoviridae phage with a C1 morphotype, isolated on host strain Burkholderia cenocepacia Van1. Phage JC1 is capable of infecting an expansive range of Burkholderia cepacia complex (Bcc) species. The JC1 genome exhibits significant similarity and synteny to Bcep22-like phages and to many Ralstonia phages. The genome of JC1 was determined to be 61,182 bp in length with a 65.4% G + C content and is predicted to encode 76 proteins and 1 tRNA gene. Unlike the other Lessieviruses, JC1 encodes a putative helicase gene in its replication module, and it is in a unique organization not found in pre...
Source: Viruses - April 29, 2022 Category: Virology Authors: Carly M. Davis Marta K. Ruest Jamie H. Cole Jonathan J. Dennis Tags: Article Source Type: research

Association between elevated peripheral blood eosinophil count and respiratory outcomes in adults with cystic fibrosis
In cystic fibrosis (CF), chronic progressive lung disease is a key contributor to patient morbidity and is the leading cause of mortality [1]. There are a number of clinical factors that have been associated with lung function decline in CF, including female sex, airway microbiology (Pseudomonas aeruginosa, Burkholderia cepacia, MRSA), CF-related diabetes, and history of pulmonary exacerbations (PEx) [2]. In terms of biological markers, sputum neutrophil elastase predicts lung function decline and the development of bronchiectasis in CF, which suggests that neutrophil airway inflammation plays a central role in CF lung dis...
Source: Journal of Cystic Fibrosis - March 31, 2022 Category: Respiratory Medicine Authors: Si Cong Ye, Sameer Desai, Emma Karlsen, Eugenie Kwong, Pearce G. Wilcox, Bradley S. Quon Source Type: research

Clinical Performance of Nanopore Targeted Sequencing for Diagnosing Infectious Diseases
Microbiol Spectr. 2022 Mar 30:e0027022. doi: 10.1128/spectrum.00270-22. Online ahead of print.ABSTRACTThe gold standard for confirming bacterial infections is culture-positive, which has a long sample-to-result turnaround time and poor sensitivity for unculturable and fastidious pathogens; therefore, it is hard to guide early, targeted antimicrobial therapy and reduce overuse of broad-spectrum antibiotics. Nanopore targeted sequencing (NTS) is reported to be advantageous in detection speed and range over culture in prior published reports. However, investigation of the clinical performance of NTS is deficient at present. T...
Source: Cancer Control - March 30, 2022 Category: Cancer & Oncology Authors: Yu Fu Qingsong Chen Mengyuan Xiong Jin Zhao Shucheng Shen Liangjun Chen Yunbao Pan Zhiqiang Li Yirong Li Source Type: research

Autophagy May Allow a Cell to Forbear Pyroptosis When Confronted With Cytosol-Invasive Bacteria
Inflammatory caspases detect cytosol-invasive Gram-negative bacteria by monitoring for the presence of LPS in the cytosol. This should provide defense against the cytosol-invasive Burkholderia and Shigella species by lysing the infected cell via pyroptosis. However, recent evidence has shown caspase-11 and gasdermin D activation can result in two different outcomes: pyroptosis and autophagy. Burkholderia cepacia complex has the ability invade the cytosol but is unable to inhibit caspase-11 and gasdermin D. Yet instead of activating pyroptosis during infection with these bacteria, the autophagy pathway is stimulated through...
Source: Frontiers in Immunology - March 29, 2022 Category: Allergy & Immunology Source Type: research

Multiresistant organisms: bacteria and beyond
Curr Opin Organ Transplant. 2022 Mar 11. doi: 10.1097/MOT.0000000000000976. Online ahead of print.ABSTRACTPURPOSE OF REVIEW: Infections with multiresistant organisms are an emerging problem, cause early mortality post lung transplantation and are sometimes associated with graft dysfunction. Frequently they raise questions about the selection of lung transplant candidates and therapeutic management post lung transplantation. There are no guidelines and management must be individualized. This review summarizes the available therapeutic options in cases of multidrug-resistant (MDR) organisms and outcomes after lung transplant...
Source: Current Opinion in Organ Transplantation - March 14, 2022 Category: Transplant Surgery Authors: Amparo Sole Source Type: research