Antibiofilm and Antimicrobial Activity of Curcumin-chitosan Nanocomplexes and Trimethoprim-sulfamethoxazole on < em > Achromobacter, Burkholderia < /em > , and < em > Stenotrophomonas isolates < /em >
CONCLUSIONS: : Cur-Chi-TPP-MNP in combination with TMP-SXT was able to inhibit biofilm and in lower effect to eradicate established biofilms of clinical isolates of A. xylosoxidans, B. cepacia complex, and S. maltophilia species. Our results highlight the need to assess these potential treatment options to be used clinically in biofilm-associated infections.PMID:36625029 | DOI:10.1080/14787210.2023.2166933 (Source: Expert Review of Anti-Infective Therapy)
Source: Expert Review of Anti-Infective Therapy - January 10, 2023 Category: Infectious Diseases Authors: Edeer Iv án Montoya-Hinojosa Humberto Antonio Salazar-Sesatty Cynthia A Alvizo-Baez Luis D Terrazas-Armendariz Itza E Luna-Cruz Juan M Alcocer-Gonz ález Licet Villarreal-Trevi ño Samantha Flores-Trevi ño Source Type: research
Microbial Epidemiology of the Cystic Fibrosis Airways: Past, Present, and Future
Semin Respir Crit Care Med. 2023 Jan 9. doi: 10.1055/s-0042-1758732. Online ahead of print.ABSTRACTProgressive obstructive lung disease secondary to chronic airway infection, coupled with impaired host immunity, is the leading cause of morbidity and mortality in cystic fibrosis (CF). Classical pathogens found in the airways of persons with CF (pwCF) include Pseudomonas aeruginosa, Staphylococcus aureus, the Burkholderia cepacia complex, Achromobacter species, and Haemophilus influenzae. While traditional respiratory-tract surveillance culturing has focused on this limited range of pathogens, the use of both comprehensive c...
Source: Respiratory Care - January 9, 2023 Category: Respiratory Medicine Authors: Christina S Thornton Michael D Parkins Source Type: research
Microbial Epidemiology of the Cystic Fibrosis Airways: Past, Present, and Future
Semin Respir Crit Care Med DOI: 10.1055/s-0042-1758732Progressive obstructive lung disease secondary to chronic airway infection, coupled with impaired host immunity, is the leading cause of morbidity and mortality in cystic fibrosis (CF). Classical pathogens found in the airways of persons with CF (pwCF) include Pseudomonas aeruginosa, Staphylococcus aureus, the Burkholderia cepacia complex, Achromobacter species, and Haemophilus influenzae. While traditional respiratory-tract surveillance culturing has focused on this limited range of pathogens, the use of both comprehensive culture and culture-independent molecular appr...
Source: Seminars in Respiratory and Critical Care Medicine - January 9, 2023 Category: Respiratory Medicine Authors: Thornton, Christina S. Parkins, Michael D. Tags: Review Article Source Type: research
Molecules, Vol. 27, Pages 8734: Palm Raceme as a Promising Biomass Precursor for Activated Carbon to Promote Lipase Activity with the Aid of Eutectic Solvents
This study concerns the role of activated carbon (AC) from palm raceme as a support material for the enhancement of lipase-catalyzed reactions in an aqueous solution, with deep eutectic solvent (DES) as a co-solvent. The effects of carbonization temperature, impregnation ratio, and carbonization time on lipase activity were studied. The activities of Amano lipase from Burkholderia cepacia (AML) and lipase from the porcine pancreas (PPL) were used to investigate the optimum conditions for AC preparation. The results showed that AC has more interaction with PPL and effectively provides greater enzymatic activity compared wit...
Source: Molecules - December 9, 2022 Category: Chemistry Authors: Khalid M. Abed Adeeb Hayyan Amal A. M. Elgharbawy Hanee F. Hizaddin Mohd Ali Hashim Hassimi Abu Hasan Mahar Diana Hamid Fathiah M. Zuki Jehad Saleh Ahmad GH Aldaihani Tags: Article Source Type: research
His-Ala-Phe-Lys peptide from Burkholderia arboris possesses antifungal activity
Burkholderia arboris, which belongs to the Burkholderia cepacia complex, has been shown to possess antifungal activity against several plant fungal pathogens; however, the antifungal compounds are yet to be identified. Here, we identified the antifungal compounds produced by B. arboris using genetic and metabolomic approaches. We generated a Tn5 transposon mutation library of 3,000 B. arboris mutants and isolated three mutants with reduced antifungal activity against the plant fungal pathogen Fusarium oxysporum. Among the mutants, the M464 mutant exhibited the weakest antifungal activity. In the M464 genome, the transposon...
Source: Frontiers in Microbiology - December 6, 2022 Category: Microbiology Source Type: research
Cephalosporins: How Vital Are They in a Hospitalist ’s Toolkit?
Case
A 57-year-old female with a history of hypertension and diabetes mellitus presented to the emergency department with complaints of chills, fever, and productive cough progressively worsening over the last five days. A physical exam revealed a well-nourished woman with mild respiratory distress and was remarkable for rhonchi heard on auscultation at the left lung base. Her blood pressure was 124/73 mm Hg, heart rate was 112 beats per minute, respiratory rate was 24 breaths per minute, and temperature was 101.2 Fahrenheit. Lab work revealed leukocytosis with bandemia. A chest X-ray reported an infiltrate in the lower lo...
Source: The Hospitalist - December 1, 2022 Category: Hospital Management Authors: Ronda Whitaker Tags: Critical Care Drug Therapy Key Clinical Questions Source Type: research
Outbreak of Burkholderia stabilis Infections Associated with Contaminated Nonsterile, Multiuse Ultrasound Gel - 10 States, May-September 2021
MMWR Morb Mortal Wkly Rep. 2022 Dec 2;71(48):1517-1521. doi: 10.15585/mmwr.mm7148a3.ABSTRACTIn July 2021, the Virginia Department of Health notified CDC of a cluster of eight invasive infections with Burkholderia stabilis, a bacterium in the Burkholderia cepacia complex (BCC), among hospitalized patients at hospital A. Most patients had undergone ultrasound-guided procedures during their admission. Culture of MediChoice M500812 nonsterile ultrasound gel used in hospital A revealed contamination of unopened product with B. stabilis that matched the whole genome sequencing (WGS) of B. stabilis strains found among patients. C...
Source: MMWR Morb Mortal Wkl... - December 1, 2022 Category: Epidemiology Authors: Matthew J Hudson Stacy C Park Amy Mathers Hardik Parikh Janet Glowicz David Dar Marjan Nabili John J LiPuma Amy Bumford Matthew A Pettengill Mark R Sterner Julie Paoline Stacy Tressler Tiina Peritz Jane Gould Stuart R Hutter Heather Moulton-Meissner Kiran Source Type: research
Clinical outcome following lung transplantation in patients with CF colonized with Burkholderia cepacia complex: results from Mater Hospital lung transplant center Dublin.
Conclusion: Infection with BCC preoperatively remains a challenging issue especially after using the common immunosupressive agents post lung trasplant with subsequent risk of sepsis. (Source: European Respiratory Journal)
Source: European Respiratory Journal - December 1, 2022 Category: Respiratory Medicine Authors: Alameeri, A., Murray, M. Tags: 08.02 - Transplantation Source Type: research
Comprehensive chiral GC-MS/MS and LC-MS/MS methods for identification and determination of N-acyl homoserine lactones
Talanta. 2022 Sep 22;253:123957. doi: 10.1016/j.talanta.2022.123957. Online ahead of print.ABSTRACTN-acyl homoserine lactones (N-HLs) are signaling molecules synthesized by gram-negative bacteria to communicate in a process called quorum sensing. Most reported methods for the analysis of N-HLs, which are chiral molecules, do not distinguish between enantiomers. Typical examples include biosensors, liquid chromatography with UV detection, gas chromatography coupled with a mass spectrometer (GC-MS) and liquid chromatography coupled with mass spectrometer (LC-MS). Recently, the production of both D,L-N-HLs have been reported ...
Source: Talanta - October 10, 2022 Category: Chemistry Authors: Ond řej Horáček Abiud E Portillo Umang Dhaubhadel Yu-Sheng Sung Elizabeth R Readel Radim Ku čera Daniel W Armstrong Source Type: research
Comprehensive chiral GC-MS/MS and LC-MS/MS methods for identification and determination of N-acyl homoserine lactones
Talanta. 2022 Sep 22;253:123957. doi: 10.1016/j.talanta.2022.123957. Online ahead of print.ABSTRACTN-acyl homoserine lactones (N-HLs) are signaling molecules synthesized by gram-negative bacteria to communicate in a process called quorum sensing. Most reported methods for the analysis of N-HLs, which are chiral molecules, do not distinguish between enantiomers. Typical examples include biosensors, liquid chromatography with UV detection, gas chromatography coupled with a mass spectrometer (GC-MS) and liquid chromatography coupled with mass spectrometer (LC-MS). Recently, the production of both D,L-N-HLs have been reported ...
Source: Talanta - October 10, 2022 Category: Chemistry Authors: Ond řej Horáček Abiud E Portillo Umang Dhaubhadel Yu-Sheng Sung Elizabeth R Readel Radim Ku čera Daniel W Armstrong Source Type: research
Comprehensive chiral GC-MS/MS and LC-MS/MS methods for identification and determination of N-acyl homoserine lactones
Talanta. 2022 Sep 22;253:123957. doi: 10.1016/j.talanta.2022.123957. Online ahead of print.ABSTRACTN-acyl homoserine lactones (N-HLs) are signaling molecules synthesized by gram-negative bacteria to communicate in a process called quorum sensing. Most reported methods for the analysis of N-HLs, which are chiral molecules, do not distinguish between enantiomers. Typical examples include biosensors, liquid chromatography with UV detection, gas chromatography coupled with a mass spectrometer (GC-MS) and liquid chromatography coupled with mass spectrometer (LC-MS). Recently, the production of both D,L-N-HLs have been reported ...
Source: Talanta - October 10, 2022 Category: Chemistry Authors: Ond řej Horáček Abiud E Portillo Umang Dhaubhadel Yu-Sheng Sung Elizabeth R Readel Radim Ku čera Daniel W Armstrong Source Type: research
Clinical characteristics, drug resistance and death risk factors of Burkholderia cepacia infection in hematopoietic stem cell transplant patients
Burkholderia cepacia (BC) has been detected more and more in infected patients in recent years. However, as a high-risk population, the clinical characteristics and prognosis of BC infection in hematopoietic stem... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - October 6, 2022 Category: Infectious Diseases Authors: Yan Jia, Ya Liu, Yi Liu, Kaitai Yang and Yanfeng Liu Tags: Research Source Type: research
482 Fourier transform infrared spectroscopy for strain typing of Burkholderia cepacia complex and monitoring of strain carriage in chronically infected people with cystic fibrosis
(Source: Journal of Cystic Fibrosis)
Source: Journal of Cystic Fibrosis - October 1, 2022 Category: Respiratory Medicine Authors: J. Tkadlec, J. Prasilova, P. Drevinek Tags: Posters Source Type: research
470 Role of cloaking antibodies in Burkholderia cepacia complex lung infections
(Source: Journal of Cystic Fibrosis)
Source: Journal of Cystic Fibrosis - October 1, 2022 Category: Respiratory Medicine Authors: A. Pham, D. Smith, L. Burr, D. Chambers, T. Wells Tags: Posters Source Type: research
Molecules, Vol. 27, Pages 5837: Identification of Coumarins and Antimicrobial Potential of Ethanolic Extracts of Dipteryx odorata and Dipteryx punctata
Castro Lustosa
Lauro Euclides Soares Barata
Dipteryx odorata and Dipteryx punctata are species native to the Amazonian, traded by extractivists to obtain coumarin. We aimed to analyze the presence of coumarin in the ethanolic extracts of leaves, branches and fruits of D. odorata and D. punctata and to evaluate the antimicrobial activity of these extracts against phytopathogenic fungi and bacteria of clinical interest. Chemical analyses were performed by thin layer chromatography (TLC) and by gas chromatography coupled to mass spectrometry (GC-MS). For the antifungal assays, the fungi used were Cercospora longissima,...
Source: Molecules - September 8, 2022 Category: Chemistry Authors: Bruna Cristine Martins de Sousa Santana Pinto de Castro Katiane Ara újo Lourido Aline Aparecida M ünchen Kasper Geomarcos da Silva Paulino Camila Delarmelina Marta Cristina Teixeira Duarte Adilson Sartoratto Thiago Almeida Vieira Denise Castro Lustosa L Tags: Article Source Type: research
