Examining the activity of cefepime-taniborbactam against < em > Burkholderia cepacia < /em > complex and < em > Burkholderia gladioli < /em > isolated from cystic fibrosis patients in the United States
Antimicrob Agents Chemother. 2023 Sep 28:e0049823. doi: 10.1128/aac.00498-23. Online ahead of print.ABSTRACTThe novel clinical-stage β-lactam-β-lactamase inhibitor combination, cefepime-taniborbactam, demonstrates promising activity toward many Gram-negative bacteria producing class A, B, C, and/or D β-lactamases. We tested this combination against a panel of 150 Burkholderia cepacia complex (Bcc) and Burkholderia gladioli strains. The addition of taniborbactam to cefepime shifted cefepime minimum inhibitory concentrations toward the provisionally susceptible range in 59% of the isolates tested. Therefore, cefepime-tani...
Source: Antimicrobial Agents and Chemotherapy - September 28, 2023 Category: Microbiology Authors: Maria F Mojica Elise T Zeiser Scott A Becka John J LiPuma David A Six Greg Moeck Krisztina M Papp-Wallace Source Type: research
When is Burkholderia cepacia complex truly eradicated in adults with cystic fibrosis? A 20-year follow up study
Burkholderia cepacia complex (BCC) infection in cystic fibrosis (CF) is associated with increased morbidity and mortality. Current UK guidance recommends segregation of people with CF according to infection status. To date there is no universally agreed consensus on the number of negative samples or time interval since last isolation of BCC for eradication to be deemed successful. (Source: Journal of Cystic Fibrosis)
Source: Journal of Cystic Fibrosis - September 28, 2023 Category: Respiratory Medicine Authors: Daniel H Tewkesbury, Louise R Pollard, Heather D Green, Alexander Horsley, Dervla Kenna, Andrew M Jones Tags: Original Article Source Type: research
Synthesis and evaluation of aromatic BDSF bioisosteres on biofilm formation and colistin sensitivity in pathogenic bacteria
Eur J Med Chem. 2023 Sep 15;261:115819. doi: 10.1016/j.ejmech.2023.115819. Online ahead of print.ABSTRACTThe diffusible signal factor family (DSF) of molecules play an important role in regulating intercellular communication, or quorum sensing, in several disease-causing bacteria. These messenger molecules, which are comprised of cis-unsaturated fatty acids, are involved in the regulation of biofilm formation, antibiotic tolerance, virulence and the control of bacterial resistance. We have previously demonstrated how olefinic N-acyl sulfonamide bioisosteric analogues of diffusible signal factor can reduce biofilm formation...
Source: European Journal of Medicinal Chemistry - September 25, 2023 Category: Chemistry Authors: Andromeda-Celeste G ómez Conor Horgan Daniel Yero Marc Bravo Xavier Daura Michelle O'Driscoll Isidre Gibert Timothy P O'Sullivan Source Type: research
Synthesis and evaluation of aromatic BDSF bioisosteres on biofilm formation and colistin sensitivity in pathogenic bacteria
Eur J Med Chem. 2023 Sep 15;261:115819. doi: 10.1016/j.ejmech.2023.115819. Online ahead of print.ABSTRACTThe diffusible signal factor family (DSF) of molecules play an important role in regulating intercellular communication, or quorum sensing, in several disease-causing bacteria. These messenger molecules, which are comprised of cis-unsaturated fatty acids, are involved in the regulation of biofilm formation, antibiotic tolerance, virulence and the control of bacterial resistance. We have previously demonstrated how olefinic N-acyl sulfonamide bioisosteric analogues of diffusible signal factor can reduce biofilm formation...
Source: European Journal of Medicinal Chemistry - September 25, 2023 Category: Chemistry Authors: Andromeda-Celeste G ómez Conor Horgan Daniel Yero Marc Bravo Xavier Daura Michelle O'Driscoll Isidre Gibert Timothy P O'Sullivan Source Type: research
Synthesis and evaluation of aromatic BDSF bioisosteres on biofilm formation and colistin sensitivity in pathogenic bacteria
Eur J Med Chem. 2023 Sep 15;261:115819. doi: 10.1016/j.ejmech.2023.115819. Online ahead of print.ABSTRACTThe diffusible signal factor family (DSF) of molecules play an important role in regulating intercellular communication, or quorum sensing, in several disease-causing bacteria. These messenger molecules, which are comprised of cis-unsaturated fatty acids, are involved in the regulation of biofilm formation, antibiotic tolerance, virulence and the control of bacterial resistance. We have previously demonstrated how olefinic N-acyl sulfonamide bioisosteric analogues of diffusible signal factor can reduce biofilm formation...
Source: European Journal of Medicinal Chemistry - September 25, 2023 Category: Chemistry Authors: Andromeda-Celeste G ómez Conor Horgan Daniel Yero Marc Bravo Xavier Daura Michelle O'Driscoll Isidre Gibert Timothy P O'Sullivan Source Type: research
Specific biomarker mining and rapid detection of Burkholderia cepacia complex by recombinase polymerase amplification
ConclusionThe automatic comparison framework for molecular biomarker mining is straightforward, universal, applicable, and efficient. Based on recognizing the BCC-specific protein SecY and its gene, we successfully established the rt-RPA assay for rapid detection in pharmaceutical and personal care products. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - September 14, 2023 Category: Microbiology Source Type: research
Comparative Genomics Identified PenR E151V Substitution Associated with Carbapenem-Resistance Burkholderia cepacia Complex and a Novel Burkholderia cepacia Complex Specific OXA-1043 Subgroup
(Source: Infection and Drug Resistance)
Source: Infection and Drug Resistance - August 28, 2023 Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
Comparison of enzymatic activities of lipases from Burkholderia plantarii and Burkholderia cepacia
Arch Microbiol. 2023 Aug 18;205(9):309. doi: 10.1007/s00203-023-03655-0.ABSTRACTLipases (EC 3.1.1.3) are enzymes used in the oils and fats industries to modify the physicochemical properties of triacylglycerol (TAG). Lipase-catalyzed interesterification at high temperatures is an effective method for modifying the physicochemical properties of TAG. The lipase from Burkholderia plantarii (BpL) exhibits excellent catalytic activity for non-regiospecific interesterification at high temperatures, with depressed lipase hydrolytic activity. The detailed catalytic mechanism for reactions involving catalytic residues has not been ...
Source: Archives of Microbiology - August 18, 2023 Category: Microbiology Authors: Saori Kataoka Sayuri Kawamoto Kazunobu Tsumura Kazuhiko Ishikawa Source Type: research
Comparison of enzymatic activities of lipases from Burkholderia plantarii and Burkholderia cepacia
Arch Microbiol. 2023 Aug 18;205(9):309. doi: 10.1007/s00203-023-03655-0.ABSTRACTLipases (EC 3.1.1.3) are enzymes used in the oils and fats industries to modify the physicochemical properties of triacylglycerol (TAG). Lipase-catalyzed interesterification at high temperatures is an effective method for modifying the physicochemical properties of TAG. The lipase from Burkholderia plantarii (BpL) exhibits excellent catalytic activity for non-regiospecific interesterification at high temperatures, with depressed lipase hydrolytic activity. The detailed catalytic mechanism for reactions involving catalytic residues has not been ...
Source: Archives of Microbiology - August 18, 2023 Category: Microbiology Authors: Saori Kataoka Sayuri Kawamoto Kazunobu Tsumura Kazuhiko Ishikawa Source Type: research
Development of a qPCR detection approach for pathogenic Burkholderia cenocepacia associated with fresh vegetables
Food Microbiol. 2023 Oct;115:104333. doi: 10.1016/j.fm.2023.104333. Epub 2023 Jul 6.ABSTRACTNatural environment serves as a reservoir for Burkholderia cepacia complex organisms, including the highly transmissible opportunistic human pathogen B. cenocepacia. Currently, there is a lack of an effective and quantitative method for B. cenocepacia detection in fresh food and other environmental niches. A quantitative real-time PCR (qPCR) detection method for B. cenocepacia bacteria was established in this study and validated using artificially inoculated fresh vegetable samples. Genome-wide comparative methods were applied to id...
Source: Food Microbiology - August 11, 2023 Category: Food Science Authors: Aixin Liu Kate Phillips Jiayuan Jia Peng Deng Dunhua Zhang Sam Chang Shi-En Lu Source Type: research
Development of a qPCR detection approach for pathogenic Burkholderia cenocepacia associated with fresh vegetables
Food Microbiol. 2023 Oct;115:104333. doi: 10.1016/j.fm.2023.104333. Epub 2023 Jul 6.ABSTRACTNatural environment serves as a reservoir for Burkholderia cepacia complex organisms, including the highly transmissible opportunistic human pathogen B. cenocepacia. Currently, there is a lack of an effective and quantitative method for B. cenocepacia detection in fresh food and other environmental niches. A quantitative real-time PCR (qPCR) detection method for B. cenocepacia bacteria was established in this study and validated using artificially inoculated fresh vegetable samples. Genome-wide comparative methods were applied to id...
Source: Food Microbiology - August 11, 2023 Category: Food Science Authors: Aixin Liu Kate Phillips Jiayuan Jia Peng Deng Dunhua Zhang Sam Chang Shi-En Lu Source Type: research
Development of a qPCR detection approach for pathogenic Burkholderia cenocepacia associated with fresh vegetables
Food Microbiol. 2023 Oct;115:104333. doi: 10.1016/j.fm.2023.104333. Epub 2023 Jul 6.ABSTRACTNatural environment serves as a reservoir for Burkholderia cepacia complex organisms, including the highly transmissible opportunistic human pathogen B. cenocepacia. Currently, there is a lack of an effective and quantitative method for B. cenocepacia detection in fresh food and other environmental niches. A quantitative real-time PCR (qPCR) detection method for B. cenocepacia bacteria was established in this study and validated using artificially inoculated fresh vegetable samples. Genome-wide comparative methods were applied to id...
Source: Food Microbiology - August 11, 2023 Category: Food Science Authors: Aixin Liu Kate Phillips Jiayuan Jia Peng Deng Dunhua Zhang Sam Chang Shi-En Lu Source Type: research
Development of a qPCR detection approach for pathogenic Burkholderia cenocepacia associated with fresh vegetables
Food Microbiol. 2023 Oct;115:104333. doi: 10.1016/j.fm.2023.104333. Epub 2023 Jul 6.ABSTRACTNatural environment serves as a reservoir for Burkholderia cepacia complex organisms, including the highly transmissible opportunistic human pathogen B. cenocepacia. Currently, there is a lack of an effective and quantitative method for B. cenocepacia detection in fresh food and other environmental niches. A quantitative real-time PCR (qPCR) detection method for B. cenocepacia bacteria was established in this study and validated using artificially inoculated fresh vegetable samples. Genome-wide comparative methods were applied to id...
Source: Food Microbiology - August 11, 2023 Category: Food Science Authors: Aixin Liu Kate Phillips Jiayuan Jia Peng Deng Dunhua Zhang Sam Chang Shi-En Lu Source Type: research
Development of a qPCR detection approach for pathogenic Burkholderia cenocepacia associated with fresh vegetables
Food Microbiol. 2023 Oct;115:104333. doi: 10.1016/j.fm.2023.104333. Epub 2023 Jul 6.ABSTRACTNatural environment serves as a reservoir for Burkholderia cepacia complex organisms, including the highly transmissible opportunistic human pathogen B. cenocepacia. Currently, there is a lack of an effective and quantitative method for B. cenocepacia detection in fresh food and other environmental niches. A quantitative real-time PCR (qPCR) detection method for B. cenocepacia bacteria was established in this study and validated using artificially inoculated fresh vegetable samples. Genome-wide comparative methods were applied to id...
Source: Food Microbiology - August 11, 2023 Category: Food Science Authors: Aixin Liu Kate Phillips Jiayuan Jia Peng Deng Dunhua Zhang Sam Chang Shi-En Lu Source Type: research
Development of a qPCR detection approach for pathogenic Burkholderia cenocepacia associated with fresh vegetables
Food Microbiol. 2023 Oct;115:104333. doi: 10.1016/j.fm.2023.104333. Epub 2023 Jul 6.ABSTRACTNatural environment serves as a reservoir for Burkholderia cepacia complex organisms, including the highly transmissible opportunistic human pathogen B. cenocepacia. Currently, there is a lack of an effective and quantitative method for B. cenocepacia detection in fresh food and other environmental niches. A quantitative real-time PCR (qPCR) detection method for B. cenocepacia bacteria was established in this study and validated using artificially inoculated fresh vegetable samples. Genome-wide comparative methods were applied to id...
Source: Food Microbiology - August 11, 2023 Category: Food Science Authors: Aixin Liu Kate Phillips Jiayuan Jia Peng Deng Dunhua Zhang Sam Chang Shi-En Lu Source Type: research
