A < em > Burkholderia cenocepacia < /em > -like environmental isolate strongly inhibits the plant fungal pathogen < em > Zymoseptoria tritici < /em >
Appl Environ Microbiol. 2024 Apr 16:e0222223. doi: 10.1128/aem.02222-23. Online ahead of print.ABSTRACTFungal phytopathogens cause significant reductions in agricultural yields annually, and overusing chemical fungicides for their control leads to environmental pollution and the emergence of resistant pathogens. Exploring natural isolates with strong antagonistic effects against pathogens can improve our understanding of their ecology and develop new treatments for the future. We isolated and characterized a novel bacterial strain associated with the species Burkholderia cenocepacia, termed APO9, which strongly inhibits Zy...
Source: Applied and Environmental Microbiology - April 16, 2024 Category: Microbiology Authors: Tingting Song Suyash Gupta Yael Sorokin Omer Frenkel Eddie Cytryn Jonathan Friedman Source Type: research
Hospital-acquired pneumonia due to Burkholderia cepacia in a thalassemia pregnancy with postoperative eclampsia: A case report
Respir Med Case Rep. 2024 Apr 4;49:102021. doi: 10.1016/j.rmcr.2024.102021. eCollection 2024.ABSTRACTThere are limited case reports on individuals infected with Burkholderia cepacia who do not have typical risk factors, particularly pregnant women with beta-thalassemia. A 34-year-old pregnant female with beta-thalassemia trait and hypertension was admitted to the hospital. The patient was diagnosed with eclampsia and underwent a cesarean section. After two days following the surgery, the patient experienced hospitality-acquired pneumonia. B.cepacia was isolated from blood cultures, and antibiotic susceptibility testing ind...
Source: Respiratory Care - April 11, 2024 Category: Respiratory Medicine Authors: Khoa Nguyen-Dang Ngoc Duong-Minh Hanh-Duyen Bui-Thi Source Type: research
Hospital-acquired pneumonia due to Burkholderia cepacia in a thalassemia pregnancy with postoperative eclampsia: A case report
Respir Med Case Rep. 2024 Apr 4;49:102021. doi: 10.1016/j.rmcr.2024.102021. eCollection 2024.ABSTRACTThere are limited case reports on individuals infected with Burkholderia cepacia who do not have typical risk factors, particularly pregnant women with beta-thalassemia. A 34-year-old pregnant female with beta-thalassemia trait and hypertension was admitted to the hospital. The patient was diagnosed with eclampsia and underwent a cesarean section. After two days following the surgery, the patient experienced hospitality-acquired pneumonia. B.cepacia was isolated from blood cultures, and antibiotic susceptibility testing ind...
Source: Respiratory Care - April 11, 2024 Category: Respiratory Medicine Authors: Khoa Nguyen-Dang Ngoc Duong-Minh Hanh-Duyen Bui-Thi Source Type: research
The evolving focus of cystic fibrosis microbiome research
For more than two decades, culture-independent microbiome analysis has been employed in efforts to improve clinical outcomes for those with CF and to gain a better fundamental understanding of polymicrobial infections. The use of these techniques began during the 1990s [1,2], in response, at least in part, to growing concerns over the emergence of new infectious syndromes. Pathogens such as Burkholderia cepacia [3] and rapidly growing nontuberculous mycobacteria [4] were increasingly associated with sudden and profound deterioration in lung function in persons with CF (PwCF) and the potential for other “novel” species ...
Source: Journal of Cystic Fibrosis - April 10, 2024 Category: Respiratory Medicine Authors: Steven L. Taylor, Geraint B. Rogers Source Type: research
Investigation of multi-infections and breast disease comorbidities in granulomatous mastitis
CONCLUSION: Multi-infections and breast disease comorbidities may further complicate diagnosis and management of GM. The findings of this study may raise additional questions about the nature of the disease or potential complications associated with it.PMID:38576970 | PMC:PMC10990350 | DOI:10.1097/MS9.0000000000001636 (Source: Annals of Medicine)
Source: Annals of Medicine - April 5, 2024 Category: Internal Medicine Authors: Nawzad Kh Esmaeil Abdulwahid M Salih Source Type: research
Non-coding regulatory sRNAs from bacteria of the Burkholderia cepacia complex
Appl Microbiol Biotechnol. 2024 Apr 2;108(1):280. doi: 10.1007/s00253-024-13121-6.ABSTRACTSmall non-coding RNAs (sRNAs) are key regulators of post-transcriptional gene expression in bacteria. Hundreds of sRNAs have been found using in silico genome analysis and experimentally based approaches in bacteria of the Burkholderia cepacia complex (Bcc). However, and despite the hundreds of sRNAs identified so far, the number of functionally characterized sRNAs from these bacteria remains very limited. In this mini-review, we describe the general characteristics of sRNAs and the main mechanisms involved in their action as regulato...
Source: Applied Microbiology and Biotechnology - April 2, 2024 Category: Microbiology Authors: Gon çalo R Matos Joana R Feliciano Jorge H Leit ão Source Type: research
Non-coding regulatory sRNAs from bacteria of the Burkholderia cepacia complex
Appl Microbiol Biotechnol. 2024 Apr 2;108(1):280. doi: 10.1007/s00253-024-13121-6.ABSTRACTSmall non-coding RNAs (sRNAs) are key regulators of post-transcriptional gene expression in bacteria. Hundreds of sRNAs have been found using in silico genome analysis and experimentally based approaches in bacteria of the Burkholderia cepacia complex (Bcc). However, and despite the hundreds of sRNAs identified so far, the number of functionally characterized sRNAs from these bacteria remains very limited. In this mini-review, we describe the general characteristics of sRNAs and the main mechanisms involved in their action as regulato...
Source: Applied Microbiology and Biotechnology - April 2, 2024 Category: Microbiology Authors: Gon çalo R Matos Joana R Feliciano Jorge H Leit ão Source Type: research
Challenges and mitigation strategies associated with Burkholderia cepacia complex contamination in pharmaceutical manufacturing
Arch Microbiol. 2024 Mar 14;206(4):159. doi: 10.1007/s00203-024-03921-9.ABSTRACTBurkholderia cepacia complex (BCC) is a Gram-negative, non-spore-forming bacterium with more than 20 opportunistic pathogenic species, most commonly found in soil and water. Due to their rapid mutation rates, these organisms are adaptable and possess high genomic plasticity. BCC can cause life-threatening infections in immunocompromised individuals, such as those with cystic fibrosis, chronic granulomatous disease, and neonates. BCC contamination is a significant concern in pharmaceutical manufacturing, frequently causing non-sterile product re...
Source: Archives of Microbiology - March 14, 2024 Category: Microbiology Authors: Sethuraman Prem Kumar Karupanagounder Thangaraj Uthra Vellapandian Chitra Narayanasamy Damodharan Gururaja Perumal Pazhani Source Type: research
Extending the computational and experimental analysis of lipase active site selectivity
In conclusion, experimental results analysis shows higher enzymatic productivity in esterification reactions of 1294.83 μmol/h.mg, while the CRL and PPL demonstrated the lowest performance (189.87 μmol / h.mg and 23.96 μmol / h.mg, respectively). Thus, molecular docking and experimental results indicate that BCL is a more efficient lipase to produce fatty acids and esters from licuri oil with a high content of lauric acid. In addition, this study also demonstrates the application of molecular docking as an important tool for lipase screening to achieve more sustainable production of butyl esters with a view synthesis of...
Source: Bioprocess and Biosystems Engineering - March 4, 2024 Category: Biomedical Engineering Authors: C ésar A Rodrigues Jefferson C B Santos Milson S Barbosa Milena C Lisboa Ranyere L Souza Adriano A Mendes Matheus M Pereira Álvaro S Lima Cleide M F Soares Source Type: research
Evaluation of the production and extraction of polyhydroxybutyrate from volatile fatty acids and using mixed cultures of PTAR and B. cepacia
Environ Res. 2024 Feb 13:118448. doi: 10.1016/j.envres.2024.118448. Online ahead of print.ABSTRACTThe global consumption of plastics generates accelerated environmental pollution in landfills and marine ecosystems. Biopolymers are the materials with the greatest potential to replace synthetic polymers in the market due to their good biodegradability, however, there are still several disadvantages, mainly related to their production cost. Considering the above, the generation of biodegradable and biocompatible bioplastics stands out as an alternative solution, some of which are made from renewable raw materials, including p...
Source: Environmental Research - February 15, 2024 Category: Environmental Health Authors: Jeniffer Gracia Armando Espinosa Nubia Moreno Iv án Cabeza Source Type: research
Evaluation of the production and extraction of polyhydroxybutyrate from volatile fatty acids and using mixed cultures of PTAR and B. cepacia
Environ Res. 2024 Feb 13:118448. doi: 10.1016/j.envres.2024.118448. Online ahead of print.ABSTRACTThe global consumption of plastics generates accelerated environmental pollution in landfills and marine ecosystems. Biopolymers are the materials with the greatest potential to replace synthetic polymers in the market due to their good biodegradability, however, there are still several disadvantages, mainly related to their production cost. Considering the above, the generation of biodegradable and biocompatible bioplastics stands out as an alternative solution, some of which are made from renewable raw materials, including p...
Source: Environmental Research - February 15, 2024 Category: Environmental Health Authors: Jeniffer Gracia Armando Espinosa Nubia Moreno Iv án Cabeza Source Type: research
Genomic editing in < em > Burkholderia multivorans < /em > by CRISPR/Cas9
Appl Environ Microbiol. 2024 Feb 2:e0225023. doi: 10.1128/aem.02250-23. Online ahead of print.ABSTRACTBurkholderia cepacia complex bacteria have emerged as opportunistic pathogens in patients with cystic fibrosis and immunocompromised individuals, causing life-threatening infections. Because of the relevance of these microorganisms, genetic manipulation is crucial for explaining the genetic mechanisms leading to pathogenesis. Despite the availability of allelic exchange tools to obtain unmarked gene deletions in Burkholderia, these require a step of merodiploid formation and another of merodiploid resolution through two in...
Source: Applied and Environmental Microbiology - February 1, 2024 Category: Microbiology Authors: Mirela R Ferreira Vasco Queiroga Leonilde M Moreira Source Type: research
Molecules, Vol. 29, Pages 628: Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization
ide M. F. Soares
This work presents a framework for evaluating hybrid nanoflowers using Burkholderia cepacia lipase. It was expanded on previous findings by testing lipase hybrid nanoflowers (hNF-lipase) formation over a wide range of pH values (5&ndash;9) and buffer concentrations (10&ndash;100 mM). The free enzyme activity was compared with that of hNF-lipase. The analysis, performed by molecular docking, described the effect of lipase interaction with copper ions. The morphological characterization of hNF-lipase was performed using scanning electron microscopy. Fourier Transform Infrared Spectroscopy...
Source: Molecules - January 29, 2024 Category: Chemistry Authors: Danivia Endi S. Souza Lucas M. F. Santos Jo ão P. A. Freitas Lays C. de Almeida Jefferson C. B. Santos Ranyere Lucena de Souza Matheus M. Pereira Álvaro S. Lima Cleide M. F. Soares Tags: Article Source Type: research
