Extending the computational and experimental analysis of lipase active site selectivity

In conclusion, experimental results analysis shows higher enzymatic productivity in esterification reactions of 1294.83 μmol/h.mg, while the CRL and PPL demonstrated the lowest performance (189.87 μmol / h.mg and 23.96 μmol / h.mg, respectively). Thus, molecular docking and experimental results indicate that BCL is a more efficient lipase to produce fatty acids and esters from licuri oil with a high content of lauric acid. In addition, this study also demonstrates the application of molecular docking as an important tool for lipase screening to achieve more sustainable production of butyl esters with a view synthesis of biolubricants.PMID:38438572 | DOI:10.1007/s00449-023-02956-4

Source: Bioprocess and Biosystems Engineering - March 4, 2024 Category: Biomedical Engineering Authors: C ésar A Rodrigues Jefferson C B Santos Milson S Barbosa Milena C Lisboa Ranyere L Souza Adriano A Mendes Matheus M Pereira Álvaro S Lima Cleide M F Soares Source Type: research