Spatial Multi-Omics in Alzheimer's Disease: A Multi-Dimensional Approach to Understanding Pathology and Progression
This article focuses on the latest advancements in spatial multi-omics technology and compares various techniques. Additionally, we provide an overview of current spatial omics-based research results in AD. These technologies play a crucial role in facilitating new discoveries and advancing translational AD research in the future. Despite challenges such as balancing resolution, increasing throughput, and data analysis, the application of spatial multi-omics holds immense potential in revolutionizing our understanding of human disease processes and identifying new biomarkers and therapeutic targets, thereby potentially con...
Source: Mol Biol Cell - May 24, 2024 Category: Molecular Biology Authors: Yixiao Ma Wenting Shi Yahong Dong Yingjie Sun Qiguan Jin Source Type: research
A Spatial Transcriptomics Browser for Discovering Gene Expression Landscapes across Microscopic Tissue Sections
Curr Issues Mol Biol. 2024 May 13;46(5):4701-4720. doi: 10.3390/cimb46050284.ABSTRACTA crucial feature of life is its spatial organization and compartmentalization on the molecular, cellular, and tissue levels. Spatial transcriptomics (ST) technology has opened a new chapter of the sequencing revolution, emerging rapidly with transformative effects across biology. This technique produces extensive and complex sequencing data, raising the need for computational methods for their comprehensive analysis and interpretation. We developed the ST browser web tool for the interactive discovery of ST images, focusing on different f...
Source: Mol Biol Cell - May 24, 2024 Category: Molecular Biology Authors: Maria Schmidt Susanna Avagyan Kristin Reiche Hans Binder Henry Loeffler-Wirth Source Type: research
CDDO, an Anti-Inflammatory and Antioxidant Compound, Attenuates Vasospasm and Neuronal Cell Apoptosis in Rats Subjected to Experimental Subarachnoid Hemorrhage
In this study, the effects of CDDO on rats subjected to SAH were evaluated. Male Sprague-Dawley rats were divided into four groups (n = 6/group): (1) control group, (2) SAH group, (3) SAH + low-dose CDDO (10 mg/kg injected into the subarachnoid space at 24 h after SAH) group, and (4) SAH + high-dose CDDO (20 mg/kg) group. CDDO improved SAH-induced poor neurological outcomes and reduced vasospasm in the basal artery following SAH. It also decreased the SAH-induced expression of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6 in both the cerebrospinal fluid and serum samples as determined by ELISA. A Western blot ...
Source: Mol Biol Cell - May 24, 2024 Category: Molecular Biology Authors: William Winardi Yun-Ping Lo Hung-Pei Tsai Yu-Hua Huang Tzu-Ting Tseng Chia-Li Chung Source Type: research
Expression of G2019S LRRK2 in Rat Primary Astrocytes Mediates Neurotoxicity and Alters the Dopamine Synthesis Pathway in N27 Cells via Astrocytic Proinflammatory Cytokines and Neurotrophic Factors
Curr Issues Mol Biol. 2024 May 6;46(5):4324-4336. doi: 10.3390/cimb46050263.ABSTRACTAstrocytes in the brain contribute to various essential functions, including maintenance of the neuronal framework, survival, communication, metabolic processes, and neurotransmitter levels. Leucine-rich repeat kinase 2 (LRRK2) is associated with the pathogenesis of Parkinson's disease (PD). LRRK2 is expressed in neurons, microglia, and astrocytes and plays diverse roles in these cell types. We aimed to determine the effects of mutant human G2019S-LRRK2 (GS-hLRRK2) in rat primary astrocytes (rASTROs). Transfection with GS-hLRRK2 significant...
Source: Mol Biol Cell - May 24, 2024 Category: Molecular Biology Authors: Dong Hwan Ho Hyejung Kim Daleum Nam Mi Kyoung Seo Sung Woo Park Ilhong Son Source Type: research
Bridging the Gap: Harnessing Plant Bioactive Molecules to Target Gut Microbiome Dysfunctions in Amyotrophic Lateral Sclerosis
Curr Issues Mol Biol. 2024 May 8;46(5):4471-4488. doi: 10.3390/cimb46050271.ABSTRACTThe correlation between neurodegenerative diseases and the gut microbiome is increasingly evident, with amyotrophic lateral sclerosis (ALS) being particularly notable for its severity and lack of therapeutic options. The gut microbiota, implicated in the pathogenesis and development of ALS, plays a crucial role in the disease. Bioactive plant molecules, specifically volatile compounds in essential oils, offer a promising therapeutic avenue due to their anti-inflammatory properties and gut-modulating effects. Our narrative review aimed to id...
Source: Mol Biol Cell - May 24, 2024 Category: Molecular Biology Authors: Mirela Pribac Anca Motataianu Sebastian Andone Elena Mardale Sebastian Nemeth Source Type: research
The Effect of Gut Microbiota-Targeted Interventions on Neuroinflammation and Motor Function in Parkinson's Disease Animal Models-A Systematic Review
Curr Issues Mol Biol. 2024 Apr 26;46(5):3946-3974. doi: 10.3390/cimb46050244.ABSTRACTGut microbiome-targeted interventions such as fecal transplant, prebiotics, probiotics, synbiotics, and antibiotic gut depletion are speculated to be of potential use in delaying the onset and progression of Parkinson's disease by rebalancing the gut microbiome in the context of the gut-brain axis. Our study aims to organize recent findings regarding these interventions in Parkinson's disease animal models to identify how they affect neuroinflammation and motor outcomes. A systematic literature search was applied in PubMed, Web of Science,...
Source: Mol Biol Cell - May 24, 2024 Category: Molecular Biology Authors: Paul- Ștefan Panaitescu Vlad R ăzniceanu Ștefania-Maria Mocrei-Rebrean Vlad Sever Neculicioiu Hanna-Maria Drago ș Carmen Costache Gabriela Adriana Filip Source Type: research
NR1D1-transactivated lncRNA NUTM2A-AS1 promotes chemoresistance and immune evasion in neuroblastoma via inhibiting B7-H3 degradation
J Cell Mol Med. 2024 May;28(10):e18360. doi: 10.1111/jcmm.18360.ABSTRACTNeuroblastoma (NB), a common solid tumour in young children originating from the sympathetic nervous system during embryonic development, poses challenges despite therapeutic advances like high-dose chemotherapy and immunotherapy. Some survivors still grapple with severe side effects and drug resistance. The role of lncRNA NUTM2A-AS1 has been explored in various cancers, but its function in drug-resistant NB progression is unclear. Our study found that NUTM2A-AS1 expression in cisplatin-resistant NB cells increased in a time- and dose-dependent manner....
Source: J Cell Mol Med - May 24, 2024 Category: Molecular Biology Authors: Tian Xiang Yejing Li Gao Liu Xianyun Li Source Type: research
Mimicking the retinal neuron functions by a photoresponsive single transistor with double-gate
Biophys J. 2024 May 23:S0006-3495(24)00350-3. doi: 10.1016/j.bpj.2024.05.023. Online ahead of print.ABSTRACTTo realize a low-cost neuromorphic visual system, employing an artificial neuron capable of mimicking the retinal neuron functions is essential. A photoresponsive single transistor (1T) neuron composed of a vertical silicon nanowire (Si-NW) is proposed. Similar to retinal neurons, various photoresponsive characteristics of the 1T neuron can be modulated by light intensity as well as wavelength, and have a high responsivity to green light like the human eye. The device is designed with a cylindrical surrounding double...
Source: Biophysical Journal - May 24, 2024 Category: Physics Authors: Qing-An Ding Chaoran Gu Jianyu Li Xiaoyuan Li BingHui Hou Yandong Peng Bing Chen Youli Yao Source Type: research
Strategies and safety simulations for ultrasonic cervical spinal cord neuromodulation
This study highlights the importance of developing trans-spine ultrasound simulation software for the assurance of subject-specific safety and efficacy of focused ultrasound spinal cord therapies.PMID:38788727 | DOI:10.1088/1361-6560/ad506f (Source: Physics in Medicine and Biology)
Source: Physics in Medicine and Biology - May 24, 2024 Category: Physics Authors: Rui Xu Sven Bestmann Bradley E Treeby Eleanor Martin Source Type: research
Mechanism of acoustic pressure spectrum shifting toward lower frequencies in applied current thermoacoustic imaging
This study aims to understand why the acoustic pressure spectrum and the final voltage signals shift towards lower frequencies in TAT.APPROACH: We employed a linear time-invariant model. In the proposed model, the applied current thermoacoustic imaging (ACTAI) processes is divided into the thermoacoustic stage and the acoustoelectric stage. These two stages are characterized by the thermoacoustic transfer function and the transducer transfer function respectively. We confirmed the effectiveness of our model through a rigorous examination involving both simulations and experiments.MAIN RESULTS: Simulation results indicate t...
Source: Physics in Medicine and Biology - May 24, 2024 Category: Physics Authors: Wenwei Zhang Hui Xia Xiaonan Li Yuanyuan Li Shiqiang Li Guoqiang Liu Source Type: research
Laminin I mediates resistance to lapatinib in HER2-positive brain metastatic breast cancer cells in vitro
Biochem Biophys Res Commun. 2024 May 18;720:150142. doi: 10.1016/j.bbrc.2024.150142. Online ahead of print.ABSTRACTThe role of extracellular matrix (ECM) prevalent in the brain metastatic breast cancer (BMBC) niche in mediating cancer cell growth, survival, and response to therapeutic agents is not well understood. Emerging evidence suggests a vital role of ECM of the primary breast tumor microenvironment (TME) in tumor progression and survival. Possibly, the BMBC cells are also similarly influenced by the ECM of the metastatic niche; therefore, understanding the effect of the metastatic ECM on BMBC cells is imperative. He...
Source: Biochemical and Biophysical Research communications - May 24, 2024 Category: Biochemistry Authors: Sumiran Kumar Gurung Lalita A Shevde Shreyas S Rao Source Type: research
Genetic Susceptibility in Endothelial Injury Syndromes after Hematopoietic Cell Transplantation and Other Cellular Therapies: Climbing a Steep Hill
Curr Issues Mol Biol. 2024 May 15;46(5):4787-4802. doi: 10.3390/cimb46050288.ABSTRACTHematopoietic stem cell transplantation (HSCT) remains a cornerstone in the management of patients with hematological malignancies. Endothelial injury syndromes, such as HSCT-associated thrombotic microangiopathy (HSCT-TMA), veno-occlusive disease/sinusoidal obstruction syndrome (SOS/VOD), and capillary leak syndrome (CLS), constitute complications after HSCT. Moreover, endothelial damage is prevalent after immunotherapy with chimeric antigen receptor-T (CAR-T) and can be manifested with cytokine release syndrome (CRS) or immune effector c...
Source: Current Issues in Molecular Biology - May 24, 2024 Category: Molecular Biology Authors: Paschalis Evangelidis Nikolaos Evangelidis Panagiotis Kalmoukos Maria Kourti Athanasios Tragiannidis Eleni Gavriilaki Source Type: research
Neuroradiology for the Ophthalmologist - Part 2 Efference
Klin Monbl Augenheilkd. 2024 May 24. doi: 10.1055/a-2312-6740. Online ahead of print.ABSTRACTEye movements are a complex task requiring a large number of structures coordinated by three cranial nerves and their centres in the brainstem. Various pathologies may affect any part of the pathway controlling the eye movements and their diagnosis is often based on history and clinical examination. Modern advances in computed tomography and magnetic resonance imaging have recently added a valuable tool in the diagnosis of oculo-motor-related diseases.PMID:38788735 | DOI:10.1055/a-2312-6740 (Source: Klinische Monatsblatter fur Augenheilkunde)
Source: Klinische Monatsblatter fur Augenheilkunde - May 24, 2024 Category: Opthalmology Authors: Aikaterini Fitsiori Heimo Steffen Source Type: research
Evaluation of the Therapeutical Effect of Matricaria Chamomilla Extract vs. Galantamine on Animal Model Memory and Behavior Using 18F-FDG PET/MRI
Curr Issues Mol Biol. 2024 May 9;46(5):4506-4518. doi: 10.3390/cimb46050273.ABSTRACTThe memory-enhancing activity of Matricaria chamomilla hydroalcoholic extract (MCE) is already being investigated by behavioral and biochemical assays in scopolamine-induced amnesia rat models, while the effects of scopolamine (Sco) on cerebral glucose metabolism are examined as well. Nevertheless, the study of the metabolic profile determined by an enriched MCE has not been performed before. The present experiments compared metabolic quantification in characteristic cerebral regions and behavioral characteristics for normal, only diseased,...
Source: Current Issues in Molecular Biology - May 24, 2024 Category: Molecular Biology Authors: Roxana Iacob Matei Palimariciuc Tudor Florea Cosmin Vasilica Pricope Cristina Mariana Uritu Bogdan Ionel Tamba Teodor Marian Ionescu Cati Raluca Stolniceanu Wael Jalloul Romeo Petru Dobrin Lucian Hritcu Oana Cioanca Monica Hancianu Alexandru Gratian Naum Source Type: research
Orexin B protects dopaminergic neurons from 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity associated with reduced extracellular signal-regulated kinase phosphorylation
CONCLUSIONS: This study demonstrated that OXB exerts a neuroprotective role associated with reduced ERK phosphorylation in the PD model. This suggests that OXB may have therapeutic potential for treatment of PD.PMID:38787465 | DOI:10.1007/s11033-024-09587-2 (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - May 24, 2024 Category: Molecular Biology Authors: Xiaodan Ma Fei Cao Jing Cui Xuezhi Li Zuojuan Yin Yili Wu Qinqin Wang Source Type: research
