Biomass hydrothermal carbonization solution-assisted synthesis of intercalation-expanded core-shell structured molybdenum disulfide for efficient adsorption of Cr (VI) in electroplating wastewater treatment
This study introduces the liquid-phase product from the hydrothermal reaction of coffee grounds (CGHCL) into the synthesis process of molybdenum disulfide, assisting in the fabrication of an intercalated, expanded core-shell structured molybdenum disulfide adsorbent (C-MoS2), designed for the adsorption and reduction of Cr (VI) from electroplating wastewater. The addition of CGHCL significantly enhances the adsorption performance of MoS2. Furthermore, C-MoS2 exhibits exceedingly high removal efficiency and excellent regenerative capability for Cr (VI)-containing electroplating wastewater. The core-shell structure effective...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Tianyu Yang Na Gao Bin Li Source Type: research

Investigating the potential of mineral precipitation in co-pyrolysis biochar: Development of a novel Cd (II) adsorption material utilizing dual solid waste
Bioresour Technol. 2024 Apr 29:130762. doi: 10.1016/j.biortech.2024.130762. Online ahead of print.ABSTRACTIonic cadmium (Cd (II)) in water is a significant threat to ecosystems, the environment, and human health. Research is currently focused on developing efficient adsorption materials to combat Cd (II) pollution in water. One promising solution involves co-pyrolyzing solid residue from anaerobic digestion of food waste with oil-based drill cuttings pyrolysis residue to create a biochar with high organic matter content. This biochar has a lower heavy metal content and leaching toxicity compared to China's national standar...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Kejin Chen Jiaojiao Jiang Chuan Huang Liao Wang Xiang Wang Source Type: research

Inhibition and disaggregation effect of flavonoid-derived carbonized polymer dots on protein amyloid aggregation
Colloids Surf B Biointerfaces. 2024 Apr 25;238:113928. doi: 10.1016/j.colsurfb.2024.113928. Online ahead of print.ABSTRACTIn this research, four water-insoluble flavonoid compounds were utilized and reacted with arginine to prepare four carbonized polymer dots with good water-solubility in a hydrothermal reactor. Structural characterization demonstrated that the prepared carbonized polymer dots were classic core-shell structure. Effect of the prepared carbonized polymer dots on protein amyloid aggregation was further investigated using hen egg white lysozyme and human lysozyme as model protein in aqueous solution. All of t...
Source: Colloids and Surfaces - May 1, 2024 Category: Biotechnology Authors: Dexin Li Sujuan Wang Jiawei Dong Jie Li Xinnan Wang Feng Liu Xinwu Ba Source Type: research

MCT4 knockdown by tumor microenvironment-responsive nanoparticles remodels the cytokine profile and eradicates aggressive breast cancer cells
Colloids Surf B Biointerfaces. 2024 Apr 25;238:113930. doi: 10.1016/j.colsurfb.2024.113930. Online ahead of print.ABSTRACTBreast cancer is a wide-spread threat to the women's health. The drawbacks of conventional treatments necessitate the development of alternative strategies, where gene therapy has regained hope in achieving an efficient eradication of aggressive tumors. Monocarboxylate transporter 4 (MCT4) plays pivotal roles in the growth and survival of various tumors, which offers a promising target for treatment. In the present study, pH-responsive lipid nanoparticles (LNPs) based on the ionizable lipid,1,2-dioleoyl...
Source: Colloids and Surfaces - May 1, 2024 Category: Biotechnology Authors: Ahmed A H Abdellatif Abdellatif Bouazzaoui Hesham M Tawfeek Mahmoud A Younis Source Type: research

Construction of atom co-sharing Bi/Bi < sub > 4 < /sub > O < sub > 5 < /sub > Br < sub > 2 < /sub > nanosheet heterojunction for plasmonic-enhanced visible-light-driven photocatalytic antibacterial activity
Colloids Surf B Biointerfaces. 2024 Apr 18;238:113923. doi: 10.1016/j.colsurfb.2024.113923. Online ahead of print.ABSTRACTThe rapid advancement of photodynamic therapy (PDT) antibacterial materials has led to promising alternatives to antibiotics for treating bacterial infections. However, antibacterial drugs have poor light absorption and utilization rates, which limits their practical application. Constructing two-dimensional (2D) heterojunctions from materials with matching photophysical properties has emerged as a highly effective strategy for achieving high-efficiency photo-antibacterial performance. Here, we designed...
Source: Colloids and Surfaces - May 1, 2024 Category: Biotechnology Authors: Guixue Zhang Jie Pan Xiaoyi Dong Xue Li Zhiguo Song Yan Liu Xiaomeng Liu Yongjin Li Qiyan Li Source Type: research

Methanotrophs mediated biogas valorization: Sustainable route to polyhydroxybutyrate production
This study explores the ability of methanotrophs to convert biogas into biopolymers, addressing H2S as a limitation in the utilization of biogas as a carbon source for bioconversion. Transcriptomic analysis was conducted to understand the growth and changes in the expression patterns of Type I and II methanotrophs under varying H2S concentrations. Results suggested that Type II methanotrophs can possess a native H2S utilization pathway. Both Type I and II methanotrophs were evaluated for their growth and polyhydroxybutyrate (PHB) production from biogas. Methylocystis sp. MJC1 and Methylocystis sp. OK1 exhibited a maximum b...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Seung Woon Hyun Shyam Krishna Tin Hoang Trung Chau Eun Yeol Lee Source Type: research

Magnetic carbon nanotubes modified with proteins and hydrophilic monomers: Cytocompatibility, in-vitro toxicity assays and permeation across biological interfaces
Int J Biol Macromol. 2024 Apr 29:131962. doi: 10.1016/j.ijbiomac.2024.131962. Online ahead of print.ABSTRACTCarbon nanotubes are promising materials for biomedical applications like delivery systems and tissue scaffolds. In this paper, magnetic carbon nanotubes (M-CNTs) covered with bovine serum albumin (M-CNTs-BSA) or functionalized with hydrophilic monomers (M-CNTs-HL) were synthesized, characterized, and evaluated concerning their interaction with Caco-2 cells. There is no comparison between these two types of functionalization, and this study aimed to verify their influence on the material's interaction with the cells....
Source: International Journal of Biological Macromolecules - May 1, 2024 Category: Biochemistry Authors: Mariana Azevedo Rosa Andreia Granja Cl áudia Nunes Salette Reis Ana Beatriz Santos da Silva Ketolly Natanne da Silva Leal Marco Aur élio Zezzi Arruda Luiz Fernando Gorup Mariane Gon çalves Santos Marcos Vinicios Salles Dias Eduardo Costa Figueiredo Source Type: research

Circular RNA KIAA0564 Serves as a Competitive Endogenous RNA for MicroRNA-424-5p, Mediating the Expression of Lysine Demethylase 4a, Thereby Facilitating Intervertebral Disc Degeneration
This study aimed to explore whether a novel circRNA, named circKIAA0564, affects nucleus pulposus (NP) cell injury and to elucidate its molecular mechanism. Both in vivo and in vitro IDD models were established, and the expression patterns of circKIAA0564/miR-424-5p/lysine demethylase 4a (KDM4A) were evaluated through quantitative reverse transcription PCR and Western blot analysis. Actinomycin D, RNase R, and Northern blotting were utilized to assess the circular structure of circKIAA0564. The Cell Counting Kit-8, flow cytometry, enzyme-linked immunosorbent assay, commercial assay kits, Western blotting, and reactive oxyg...
Source: Applied Biochemistry and Biotechnology - May 1, 2024 Category: Biochemistry Authors: Peng Zeng XianBo Zheng Hui Zhang MingHan Zhou Zhen Chen HanLin Song WuJi Xu Source Type: research

Gram-scale approach for β-costic acid via allylic oxidation of β-selinene
Biosci Biotechnol Biochem. 2024 May 1:zbae052. doi: 10.1093/bbb/zbae052. Online ahead of print.ABSTRACTβ-Costic acid is a sesquiterpene phytoalexin with acaricidal activity against Varroa destructor and antitrypanosomal activity. A concise and efficient method was developed for the synthesis of β-costic acid via the allylic oxidation of β-selinene, a component of celery seed oil.PMID:38692843 | DOI:10.1093/bbb/zbae052 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - May 1, 2024 Category: Biochemistry Authors: Yoshitaka Matsushima Kosuke Iwata Source Type: research

Morphological Engineering of Filamentous Fungi: Research Progress and Perspectives
J Microbiol Biotechnol. 2024 Mar 26;34(6):1-9. doi: 10.4014/jmb.2402.02007. Online ahead of print.ABSTRACTFilamentous fungi are important cell factories for the production of high-value enzymes and chemicals for the food, chemical, and pharmaceutical industries. Under submerged fermentation, filamentous fungi exhibit diverse fungal morphologies that are influenced by environmental factors, which in turn affect the rheological properties and mass transfer of the fermentation system, and ultimately the synthesis of products. In this review, we first summarize the mechanisms of mycelial morphogenesis and then provide an overv...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Zhengwu Lu Zhiqun Chen Yunguo Liu Xuexue Hua Cuijuan Gao Jingjing Liu Source Type: research

Research Progress on Strategies for Improving the Enzyme Properties of Bacteriophage Endolysins
J Microbiol Biotechnol. 2024 Feb 28;34(6):1-8. doi: 10.4014/jmb.2312.12050. Online ahead of print.ABSTRACTBacterial resistance to commonly used antibiotics is one of the major challenges to be solved today. Bacteriophage endolysins (Lysins) have become a hot research topic as a new class of antibacterial agents. They have promising applications in bacterial infection prevention and control in multiple fields, such as livestock and poultry farming, food safety, clinical medicine and pathogen detection. However, many phage endolysins display low bactericidal activities, short half-life and narrow lytic spectrums. Therefore, ...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Yulu Wang Xue Wang Xin Liu Bokun Lin Source Type: research

Novel Qnr Families as Conserved and Intrinsic Quinolone Resistance Determinants in < em > Aeromonas < /em > spp
In this study, five environmental strains of Aeromonas spp. exhibiting multidrug resistance (MDR) were selected for whole-genome sequencing to ascertain their taxonomic assignment at the species-level and to delineate their ARG repertoires. Analyses of their genomes revealed the presence of one protein almost identical to AhQnr (A. hydrophila Qnr protein) and four novel proteins similar to AhQnr. To scrutinize the classification and taxonomic distribution of these proteins, all Aeromonas genomes deposited in the NCBI RefSeq genome database (1,222 genomes) were investigated. This revealed that these Aeromonas Qnr (AQnr) pro...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Sang-Gyu Kim Bo-Eun Kim Jung Hun Lee Dae-Wi Kim Source Type: research

Evaluating the Nutritional Composition of Unripe Citrus and Its Effect on Inhibiting Adipogenesis and Adipocyte Differentiation
In this study, we assessed the nutritional components of unripe citrus peel and pressed juices, as well as their anti-obesity potential through the modulation of adipocyte differentiation and the expression of adipogenesisrelated genes, specifically PPARγ and C/EBPα, in 3T3-L1 preadipocytes. Our analysis revealed that unripe citrus peel exhibited elevated levels of fiber and protein compared to pressed juice, with markedly low levels of free sugar, particularly sucrose. The content of hesperidin, a representative flavonoid in citrus fruits, was 3,157.6 mg/kg in unripe citrus peel and 455.5 mg/kg in pressed juice, indicat...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Sunghee Kim Eunbi Lee Juhye Park Ju-Ock Nam Soo Rin Kim Source Type: research

A Novel Thermo-Alkaline Stable GDSL/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea  Pseudomonas sp
In this study, we report a new esterase (EstGoM) from a marine Pseudomonas sp. isolated at a depth of 1000 m in the Gulf of Mexico. Bioinformatic analyses revealed that EstGoM is an autotransporter esterase (type Va) and belongs to the lipolytic family II, forming a new subgroup. The purified recombinant EstGoM, with a molecular mass of 67.4 kDa, showed the highest hydrolytic activity with p-nitrophenyl octanoate (p-NP C8), although it was also active against p-NP C4, C5, C10, and C12. The optimum pH and temperature for EstGoM were 9 and 60 °C, respectively, but it retained more than 50% of its activity over the pH range ...
Source: Marine Biotechnology - May 1, 2024 Category: Biotechnology Authors: Jos é Luis Rodríguez-Mejía Itzel Anah í Hidalgo-Manzano Luis Felipe Muriel-Mill án Nancy Rivera-Gomez Diana X Sahonero-Canavesi Edmundo Castillo Liliana Pardo-L ópez Source Type: research

Reproductive Characteristics and Suitability of Sterile dead end Knockout Nibe Croaker as a Recipient for Intraperitoneal Germ Cell Transplantation
In this study, nibe croaker was utilized as a model for marine teleosts that produce small pelagic eggs, and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system was utilized to produce dnd KO fish. The F1 generation, which carried a nonsense mutation in the dnd gene, was produced by mating founder individuals with wild-type counterparts. Subsequently, the F2 generation was produced by mating the resulting males and females. Among the F2 generations, 24.0% consisted of homozygous KO individuals. Histological analysis revealed that primordial germ cells (PGCs...
Source: Marine Biotechnology - May 1, 2024 Category: Biotechnology Authors: Ryosuke Yazawa Kyoichiro Saitoh Akihiro Yamauchi Onur Ey üboğlu Kana Ozawa Wataru Kawamura Tetsuro Morita Yutaka Takeuchi Goro Yoshizaki Source Type: research