Recombinant cellobiose dehydrogenase from Thermothelomyces thermophilus: Its functional characterization and applicability in cellobionic acid production
Bioresour Technol. 2024 Apr 29:130763. doi: 10.1016/j.biortech.2024.130763. Online ahead of print.ABSTRACTThe fungus Thermothelomyces thermophilus is a thermotolerant microorganism that has been explored as a reservoir for enzymes (hydrolytic enzymes and oxidoreductases). The functional analysis of a recombinant cellobiose dehydrogenase (MtCDHB) from T. thermophilus demonstrated a thermophilic behavior, an optimal pH in alkaline conditions for inter-domain electron transfer, and catalytic activity on cellooligosaccharides of various sizes. Its applicability was evaluated to the sustainable production of cellobionic acid (C...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Bianca Oliva Josman Velasco Gabriela Leila Berto Igor Polikarpov Leandro Cristante de Oliveira Fernando Segato Source Type: research
Assessing microbial stability and predicting biogas production in full-scale thermophilic dry methane fermentation of municipal solid waste
Bioresour Technol. 2024 Apr 29:130766. doi: 10.1016/j.biortech.2024.130766. Online ahead of print.ABSTRACTCompared to typical anaerobic digestion processes, little is known about both sludge microbial compositions and biogas production models for full-scale dry methane fermentation treating municipal solid waste (MSW). The anaerobic sludge composed of one major hydrogenotrophic methanogen (Methanoculleus) and syntrophic acetate oxidizing bacteria (e.g., Caldicoprobacter), besides enrichment of MSW degraders such as Clostridia. The core population remained phylogenetically unchanged during the fermentation process, regardle...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Yuya Sato Kentaro Hasemi Kazunori Machikawa Hisato Kinjo Naohisa Yashiro Yosuke Iimura Hiroshi Aoki Hiroshi Habe Source Type: research
Absorbing oxygen carriers promotes phosphorus recovery from sludge via the microwave thermal conversion process
This study aims to apply the Absorbing oxygen carriers (AOCs) to induce the migration and transformation of phosphorus compounds during the microwave thermal conversion of sludge so the hard-to-extract organic phosphorus (OP) can be converted to easy-to-extract inorganic phosphorus (IP) and be enriched onto the sludge char. The AOCs were recycled by screen separation from the IP-rich sludge char, with the latter being a renewable phosphorus source from sludge. The AOCs in this novel process enhanced the conversion efficiency of OP into non-apatite inorganic phosphorus (NAlP), which was further converted to apatite inorgani...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Xiaoyu Yan Shuheng Zhao Jianjun Hu Jiatao Dang Sen Yao Junmeng Cai Sureewan Sittijunda Wei Wang Duu-Jong Lee Panbo Yang Source Type: research
Unveiling long-term combined effect of salinity and Lead(II) on anammox activity and microbial community dynamics in saline wastewater treatment
This study offers insights into anammox operation for treating saline wastewater with heavy metals.PMID:38692373 | DOI:10.1016/j.biortech.2024.130767 (Source: Bioresource Technology)
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Wenjun Yin Yilin Li Wei Xu Yibin Bao Junjie Zhu Xiaomei Su Jie Han Chongjun Chen Hongjun Lin Faqian Sun Source Type: research
Pre-exposure of Triclosan compromise tetracycline-derived antibiotic resistance in methanogenic digestion microbiome
This study provides new insights into the impact of antibacterial agents on the methanogenic digestion microbiome.PMID:38692374 | DOI:10.1016/j.biortech.2024.130758 (Source: Bioresource Technology)
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Xiangping Xu Haihong Jiang Qihong Lu Shanquan Wang Source Type: research
Effect of hydrothermal-acid pretreatment on methane yield and microbial community in anaerobic digestion of rice straw
This study identified optimal pretreatment at 120 °C with 3 % acetic acid for 24 h by orthogonal design method. The HTOAP increased the reducing sugar content by destroying the lignocellulosic structure. A 79 % increment of methane production after HTOAP was observed compared to the untreated group. Microbial analysis showed that HTOAP enriched the relative abundance of lignocellulose-degraders, such as W5053, Thermanaerovibrio, Caldicoprobacter, as well as the syntrophic acetate oxidizing bacteria Syntrophaceticus. Moreover, Methanobacterium conducted hydrogenotrophic methanogenesis dominantly. Furthermore, the potential...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Wei Luo Hailin Tian Wenxia Tan Qian Tan Source Type: research
Biomass hydrothermal carbonization solution-assisted synthesis of intercalation-expanded core-shell structured molybdenum disulfide for efficient adsorption of Cr (VI) in electroplating wastewater treatment
This study introduces the liquid-phase product from the hydrothermal reaction of coffee grounds (CGHCL) into the synthesis process of molybdenum disulfide, assisting in the fabrication of an intercalated, expanded core-shell structured molybdenum disulfide adsorbent (C-MoS2), designed for the adsorption and reduction of Cr (VI) from electroplating wastewater. The addition of CGHCL significantly enhances the adsorption performance of MoS2. Furthermore, C-MoS2 exhibits exceedingly high removal efficiency and excellent regenerative capability for Cr (VI)-containing electroplating wastewater. The core-shell structure effective...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Tianyu Yang Na Gao Bin Li Source Type: research
Investigating the potential of mineral precipitation in co-pyrolysis biochar: Development of a novel Cd (II) adsorption material utilizing dual solid waste
Bioresour Technol. 2024 Apr 29:130762. doi: 10.1016/j.biortech.2024.130762. Online ahead of print.ABSTRACTIonic cadmium (Cd (II)) in water is a significant threat to ecosystems, the environment, and human health. Research is currently focused on developing efficient adsorption materials to combat Cd (II) pollution in water. One promising solution involves co-pyrolyzing solid residue from anaerobic digestion of food waste with oil-based drill cuttings pyrolysis residue to create a biochar with high organic matter content. This biochar has a lower heavy metal content and leaching toxicity compared to China's national standar...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Kejin Chen Jiaojiao Jiang Chuan Huang Liao Wang Xiang Wang Source Type: research
Inhibition and disaggregation effect of flavonoid-derived carbonized polymer dots on protein amyloid aggregation
Colloids Surf B Biointerfaces. 2024 Apr 25;238:113928. doi: 10.1016/j.colsurfb.2024.113928. Online ahead of print.ABSTRACTIn this research, four water-insoluble flavonoid compounds were utilized and reacted with arginine to prepare four carbonized polymer dots with good water-solubility in a hydrothermal reactor. Structural characterization demonstrated that the prepared carbonized polymer dots were classic core-shell structure. Effect of the prepared carbonized polymer dots on protein amyloid aggregation was further investigated using hen egg white lysozyme and human lysozyme as model protein in aqueous solution. All of t...
Source: Colloids and Surfaces - May 1, 2024 Category: Biotechnology Authors: Dexin Li Sujuan Wang Jiawei Dong Jie Li Xinnan Wang Feng Liu Xinwu Ba Source Type: research
MCT4 knockdown by tumor microenvironment-responsive nanoparticles remodels the cytokine profile and eradicates aggressive breast cancer cells
Colloids Surf B Biointerfaces. 2024 Apr 25;238:113930. doi: 10.1016/j.colsurfb.2024.113930. Online ahead of print.ABSTRACTBreast cancer is a wide-spread threat to the women's health. The drawbacks of conventional treatments necessitate the development of alternative strategies, where gene therapy has regained hope in achieving an efficient eradication of aggressive tumors. Monocarboxylate transporter 4 (MCT4) plays pivotal roles in the growth and survival of various tumors, which offers a promising target for treatment. In the present study, pH-responsive lipid nanoparticles (LNPs) based on the ionizable lipid,1,2-dioleoyl...
Source: Colloids and Surfaces - May 1, 2024 Category: Biotechnology Authors: Ahmed A H Abdellatif Abdellatif Bouazzaoui Hesham M Tawfeek Mahmoud A Younis Source Type: research
Construction of atom co-sharing Bi/Bi < sub > 4 < /sub > O < sub > 5 < /sub > Br < sub > 2 < /sub > nanosheet heterojunction for plasmonic-enhanced visible-light-driven photocatalytic antibacterial activity
Colloids Surf B Biointerfaces. 2024 Apr 18;238:113923. doi: 10.1016/j.colsurfb.2024.113923. Online ahead of print.ABSTRACTThe rapid advancement of photodynamic therapy (PDT) antibacterial materials has led to promising alternatives to antibiotics for treating bacterial infections. However, antibacterial drugs have poor light absorption and utilization rates, which limits their practical application. Constructing two-dimensional (2D) heterojunctions from materials with matching photophysical properties has emerged as a highly effective strategy for achieving high-efficiency photo-antibacterial performance. Here, we designed...
Source: Colloids and Surfaces - May 1, 2024 Category: Biotechnology Authors: Guixue Zhang Jie Pan Xiaoyi Dong Xue Li Zhiguo Song Yan Liu Xiaomeng Liu Yongjin Li Qiyan Li Source Type: research
Methanotrophs mediated biogas valorization: Sustainable route to polyhydroxybutyrate production
This study explores the ability of methanotrophs to convert biogas into biopolymers, addressing H2S as a limitation in the utilization of biogas as a carbon source for bioconversion. Transcriptomic analysis was conducted to understand the growth and changes in the expression patterns of Type I and II methanotrophs under varying H2S concentrations. Results suggested that Type II methanotrophs can possess a native H2S utilization pathway. Both Type I and II methanotrophs were evaluated for their growth and polyhydroxybutyrate (PHB) production from biogas. Methylocystis sp. MJC1 and Methylocystis sp. OK1 exhibited a maximum b...
Source: Bioresource Technology - May 1, 2024 Category: Biotechnology Authors: Seung Woon Hyun Shyam Krishna Tin Hoang Trung Chau Eun Yeol Lee Source Type: research
Magnetic carbon nanotubes modified with proteins and hydrophilic monomers: Cytocompatibility, in-vitro toxicity assays and permeation across biological interfaces
Int J Biol Macromol. 2024 Apr 29:131962. doi: 10.1016/j.ijbiomac.2024.131962. Online ahead of print.ABSTRACTCarbon nanotubes are promising materials for biomedical applications like delivery systems and tissue scaffolds. In this paper, magnetic carbon nanotubes (M-CNTs) covered with bovine serum albumin (M-CNTs-BSA) or functionalized with hydrophilic monomers (M-CNTs-HL) were synthesized, characterized, and evaluated concerning their interaction with Caco-2 cells. There is no comparison between these two types of functionalization, and this study aimed to verify their influence on the material's interaction with the cells....
Source: International Journal of Biological Macromolecules - May 1, 2024 Category: Biochemistry Authors: Mariana Azevedo Rosa Andreia Granja Cl áudia Nunes Salette Reis Ana Beatriz Santos da Silva Ketolly Natanne da Silva Leal Marco Aur élio Zezzi Arruda Luiz Fernando Gorup Mariane Gon çalves Santos Marcos Vinicios Salles Dias Eduardo Costa Figueiredo Source Type: research
Circular RNA KIAA0564 Serves as a Competitive Endogenous RNA for MicroRNA-424-5p, Mediating the Expression of Lysine Demethylase 4a, Thereby Facilitating Intervertebral Disc Degeneration
This study aimed to explore whether a novel circRNA, named circKIAA0564, affects nucleus pulposus (NP) cell injury and to elucidate its molecular mechanism. Both in vivo and in vitro IDD models were established, and the expression patterns of circKIAA0564/miR-424-5p/lysine demethylase 4a (KDM4A) were evaluated through quantitative reverse transcription PCR and Western blot analysis. Actinomycin D, RNase R, and Northern blotting were utilized to assess the circular structure of circKIAA0564. The Cell Counting Kit-8, flow cytometry, enzyme-linked immunosorbent assay, commercial assay kits, Western blotting, and reactive oxyg...
Source: Applied Biochemistry and Biotechnology - May 1, 2024 Category: Biochemistry Authors: Peng Zeng XianBo Zheng Hui Zhang MingHan Zhou Zhen Chen HanLin Song WuJi Xu Source Type: research
Gram-scale approach for β-costic acid via allylic oxidation of β-selinene
Biosci Biotechnol Biochem. 2024 May 1:zbae052. doi: 10.1093/bbb/zbae052. Online ahead of print.ABSTRACTβ-Costic acid is a sesquiterpene phytoalexin with acaricidal activity against Varroa destructor and antitrypanosomal activity. A concise and efficient method was developed for the synthesis of β-costic acid via the allylic oxidation of β-selinene, a component of celery seed oil.PMID:38692843 | DOI:10.1093/bbb/zbae052 (Source: Bioscience, Biotechnology, and Biochemistry)
Source: Bioscience, Biotechnology, and Biochemistry - May 1, 2024 Category: Biochemistry Authors: Yoshitaka Matsushima Kosuke Iwata Source Type: research
