Circular RNA KIAA0564 Serves as a Competitive Endogenous RNA for MicroRNA-424-5p, Mediating the Expression of Lysine Demethylase 4a, Thereby Facilitating Intervertebral Disc Degeneration
This study aimed to explore whether a novel circRNA, named circKIAA0564, affects nucleus pulposus (NP) cell injury and to elucidate its molecular mechanism. Both in vivo and in vitro IDD models were established, and the expression patterns of circKIAA0564/miR-424-5p/lysine demethylase 4a (KDM4A) were evaluated through quantitative reverse transcription PCR and Western blot analysis. Actinomycin D, RNase R, and Northern blotting were utilized to assess the circular structure of circKIAA0564. The Cell Counting Kit-8, flow cytometry, enzyme-linked immunosorbent assay, commercial assay kits, Western blotting, and reactive oxyg...
Source: Applied Biochemistry and Biotechnology - May 1, 2024 Category: Biochemistry Authors: Peng Zeng XianBo Zheng Hui Zhang MingHan Zhou Zhen Chen HanLin Song WuJi Xu Source Type: research
Integrated micro ‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform
This study is based upon the design and testing of a HTPD platform for integration of the protein A and low pH VI operations. This was achieved by using a design and simulation software before execution on an automated liquid handler. The operations were successfully translated to the micro-scale, as assessed by analysis of recoveries and molecular weight content. The integrated platform was then used as a tool to assess the effect of pH on HMWC during low pH hold. The laboratory-scale and micro-scale elution pools showed comparable HMWC across the pH range 3.2 –3.7. The investigative power of the platform is highlighted...
Source: Biotechnology Progress - May 1, 2024 Category: Biotechnology Authors: Paras Sharma,
Lars Robbel,
Michael Schmitt,
Duygu Dikicioglu,
Daniel G. Bracewell Tags: RESEARCH ARTICLE Source Type: research
Synergistic effects of miR ‐143 with miR‐99a inhibited cell proliferation and induced apoptosis in breast cancer
In this study, the precursor structure of miRNAs in the expression vector pEGFP-N1 entered single and double states, and MCF7 and T47D cells were transfected. The miRNAs expression level after transfection was then measured using qPCR. The MultiMiR package was used to obtain predicted and validated miRNA targets. MTT assay, qRT-PCR, migration test, and flow cytometry were used to assess the effect of miRNA and gene modulation. The qPCR results revealed that miRNA constructs were significantly expressed after the transfection of both cell lines. The biological function of miRNAs showed that upregulation of miR-99a and miR-1...
Source: Biotechnology and Applied Biochemistry - May 1, 2024 Category: Biochemistry Authors: Zahra Doosti,
Syed Omar Ebrahimi,
Mahshid Samie Ghahfarokhi,
Somayeh Reiisi Tags: ORIGINAL ARTICLE Source Type: research
Quantitative detection of mpox antigen using time ‐resolved fluorescence immunochromatography
This study aims to establish a time-resolved fluorescence immunochromatography (TRFICO) method for qualitative/quantitative detection of mpox virus. A double-antibody sandwich TRFICO method was optimized and established using mpox recombinant fusion antigen and its paired monoclonal antibody. The test performance of the method was evaluated using mpox fusion antigen and control serum, including sensitivity, linearity range, specificity, precision, and reference interval. We successfully established a TRFICO method for qualitative/quantitative detection of mpox antigen, its linearity range 0 –100 ng/mL, analytical sensit...
Source: Biotechnology and Applied Biochemistry - May 1, 2024 Category: Biochemistry Authors: Huankun Liang,
Cuicui Chen,
Tiancai Liu,
Wenqi Dong,
Laiqing Li Tags: ORIGINAL ARTICLE Source Type: research
Purification and characterization of glutamate dehydrogenase from rainbow trout (Oncorhynchus mykiss) liver and molecular docking studies
In this study, GDH enzyme was purified from the liver of rainbow trout (Oncorhynchus mykiss) by 2',5'-ADP Sepharose 4B affinity chromatography in one step. As a result of this purification process, GDH enzyme was purified 171-fold with 5.83 U/mg protein-specific activity. The characterization experiments presented that the storage stability of the purified GDH enzyme was determined as −80°C; optimum temperature 40°C; it was determined that the optimum ionic strength was 100 mM phosphate buffer and the optimum pH was 8.00. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and PAGE studies showed th...
Source: Biotechnology and Applied Biochemistry - May 1, 2024 Category: Biochemistry Authors: Onur Ertik,
Refiye Yanardag Tags: ORIGINAL ARTICLE Source Type: research
Structural and biochemical characterization of the M405S variant of Desulfovibrio vulgaris formate dehydrogenase
Molybdenum- or tungsten-dependent formate dehydrogenases have emerged as significant catalysts for the chemical reduction of CO2 to formate, with biotechnological applications envisaged in climate-change mitigation. The role of Met405 in the active site of Desulfovibrio vulgaris formate dehydrogenase AB (DvFdhAB) has remained elusive. However, its proximity to the metal site and the conformational change that it undergoes between the resting and active forms suggests a functional role. In this work, the M405S variant was engineered, which allowed the active-site geometry in the absence of methionine S δ interactions wi...
Source: Acta Crystallographica Section F - May 1, 2024 Category: Biochemistry Authors: Vilela-Alves, G. Rebelo Manuel, R. Pedrosa, N. Cardoso Pereira, I.A. Rom ã o, M.J. Mota, C. Tags: CO2 reduction metal-dependent formate dehydrogenases Desulfovibrio vulgaris Mo/W enzymes X-ray crystallography catalysis research communications Source Type: research
Sensors, Vol. 24, Pages 2904: Recent Trends and Innovations in Bead-Based Biosensors for Cancer Detection
Chuang
Demand is strong for sensitive, reliable, and cost-effective diagnostic tools for cancer detection. Accordingly, bead-based biosensors have emerged in recent years as promising diagnostic platforms based on wide-ranging cancer biomarkers owing to the versatility, high sensitivity, and flexibility to perform the multiplexing of beads. This comprehensive review highlights recent trends and innovations in the development of bead-based biosensors for cancer-biomarker detection. We introduce various types of bead-based biosensors such as optical, electrochemical, and magnetic biosensors, along with their respective ...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Hui-Pin Cheng Tai-Hua Yang Jhih-Cheng Wang Han-Sheng Chuang Tags: Review Source Type: research
Sensors, Vol. 24, Pages 2903: Advancements in Polymer-Assisted Layer-by-Layer Fabrication of Wearable Sensors for Health Monitoring
ng-Te Lin
Li Fu
Recent advancements in polymer-assisted layer-by-layer (LbL) fabrication have revolutionized the development of wearable sensors for health monitoring. LbL self-assembly has emerged as a powerful and versatile technique for creating conformal, flexible, and multi-functional films on various substrates, making it particularly suitable for fabricating wearable sensors. The incorporation of polymers, both natural and synthetic, has played a crucial role in enhancing the performance, stability, and biocompatibility of these sensors. This review provides a comprehensive overview of the principles of LbL se...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Meiqing Jin Peizheng Shi Zhuang Sun Ningbin Zhao Mingjiao Shi Mengfan Wu Chen Ye Cheng-Te Lin Li Fu Tags: Review Source Type: research
Sensors, Vol. 24, Pages 2899: Bat2Web: A Framework for Real-Time Classification of Bat Species Echolocation Signals Using Audio Sensor Data
Dana Dghaym
Bats play a pivotal role in maintaining ecological balance, and studying their behaviors offers vital insights into environmental health and aids in conservation efforts. Determining the presence of various bat species in an environment is essential for many bat studies. Specialized audio sensors can be used to record bat echolocation calls that can then be used to identify bat species. However, the complexity of bat calls presents a significant challenge, necessitating expert analysis and extensive time for accurate interpretation. Recent advances in neural networks can help identify bat species automatica...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Taslim Mahbub Azadan Bhagwagar Priyanka Chand Imran Zualkernan Jacky Judas Dana Dghaym Tags: Article Source Type: research
Sensors, Vol. 24, Pages 2905: YOLOv8-MU: An Improved YOLOv8 Underwater Detector Based on a Large Kernel Block and a Multi-Branch Reparameterization Module
wen Zhang
Underwater visual detection technology is crucial for marine exploration and monitoring. Given the growing demand for accurate underwater target recognition, this study introduces an innovative architecture, YOLOv8-MU, which significantly enhances the detection accuracy. This model incorporates the large kernel block (LarK block) from UniRepLKNet to optimize the backbone network, achieving a broader receptive field without increasing the model’s depth. Additionally, the integration of C2fSTR, which combines the Swin transformer with the C2f module, and the SPPFCSPC_EMA module, which blends Cross...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Xing Jiang Xiting Zhuang Jisheng Chen Jian Zhang Yiwen Zhang Tags: Article Source Type: research
Sensors, Vol. 24, Pages 2900: Soft Sensory-Motor System Based on Ionic Solution for Robotic Applications
This study provides a comparative analysis of the performance of SSMS-IS prototypes with three diverse designs: toroidal, semi-toroidal, and rectangular geometries, based on a series of performance experiments, such as sensitivity, drift, and durability. The design with the best performance was the rectangular SSMS-IS using silicon rubber RPRO20 for both internal and external pressures applied in the system. Moreover, this work explores the performance of a bioinspired soft robot using rectangular SSMS-IS elements integrated in its body. Further, it investigated the feasibility of the robot to adapt its morphology online f...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Sender Rocha dos Santos Eric Rohmer Tags: Article Source Type: research
Sensors, Vol. 24, Pages 2902: Wide Voltage Swing Potentiostat with Dynamic Analog Ground to Expand Electrochemical Potential Windows in Integrated Microsystems
. Mason
Electrochemical measurements are vital to a wide range of applications such as air quality monitoring, biological testing, food industry, and more. Integrated circuits have been used to implement miniaturized and low-power electrochemical potentiostats that are suitable for wearable devices. However, employing modern integrated circuit technologies with low supply voltage precludes the utilization of electrochemical reactions that require a higher potential window. In this paper, we present a novel circuit architecture that utilizes dynamic voltage at the working electrode of an electrochemical cell to effectiv...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Ehsan Ashoori Derek Goderis Anna Inohara Andrew J. Mason Tags: Article Source Type: research
Sensors, Vol. 24, Pages 2901: Imaging of Structural Timber Based on In Situ Radar and Ultrasonic Wave Measurements: A Review of the State-of-the-Art
Sinha
With the rapidly growing interest in using structural timber, a need exists to inspect and assess these structures using non-destructive testing (NDT). This review article summarizes NDT methods for wood inspection. After an overview of the most important NDT methods currently used, a detailed review of Ground Penetrating Radar (GPR) and Ultrasonic Testing (UST) is presented. These two techniques can be applied in situ and produce useful visual representations for quantitative assessments and damage detection. With its commercial availability and portability, GPR can help rapidly identify critical features such a...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Narges Pahnabi Thomas Schumacher Arijit Sinha Tags: Review Source Type: research
Sensors, Vol. 24, Pages 2897: A Survey on Satellite Communication System Security
n Lee
In recent years, satellite communication systems (SCSs) have rapidly developed in terms of their role and capabilities, promoted by advancements in space launch technologies. However, this rapid development has also led to the emergence of significant security vulnerabilities, demonstrated through real-world targeted attacks such as AcidRain and AcidPour that demand immediate attention from the security community. In response, various countermeasures, encompassing both technological and policy-based approaches, have been proposed to mitigate these threats. However, the multitude and diversity of these proposals m...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Minjae Kang Sungbin Park Yeonjoon Lee Tags: Review Source Type: research
Sensors, Vol. 24, Pages 2898: Edge Caching Data Distribution Strategy with Minimum Energy Consumption
ang
In the context of the rapid development of the Internet of Vehicles, virtual reality, automatic driving and the industrial Internet, the terminal devices in the network show explosive growth. As a result, more and more information is generated from the edge of the network, which makes the data throughput increase dramatically in the mobile communication network. As the key technology of the fifth-generation mobile communication network, mobile edge caching technology which caches popular data to the edge server deployed at the edge of the network avoids the data transmission delay of the backhaul link and the occur...
Source: Sensors - May 1, 2024 Category: Biotechnology Authors: Zhi Lin Jiarong Liang Tags: Article Source Type: research
