Conducting Polymer ‐Based Electrochemical Biosensor for the Detection of Acetylthiocoline and Pesticides via Acetylcholinesterase
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 17, 2020 Category: Biochemistry Authors: Abdurrahman Akdag, Mesut I şık, Hasan Göktaş Tags: Original Article Source Type: research

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 17, 2020 Category: Biochemistry Authors: Patrycia G. M. Castro, Roberto N. Maeda, Vanessa A. L. Rocha, Rodrigo P. Fernandes, Nei Pereira Jr Tags: Original Article Source Type: research

Conducting Polymer ‐Based Electrochemical Biosensor for the Detection of Acetylthiocoline and Pesticides via Acetylcholinesterase
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 17, 2020 Category: Biochemistry Authors: Abdurrahman Akdag, Mesut I şık, Hasan Göktaş Tags: Original Article Source Type: research

Improving propionic acid production from a hemicellulosic hydrolysate of sorghum bagasse by means of cell immobilization and sequential batch operation
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 17, 2020 Category: Biochemistry Authors: Patrycia G. M. Castro, Roberto N. Maeda, Vanessa A. L. Rocha, Rodrigo P. Fernandes, Nei Pereira Jr Tags: Original Article Source Type: research

Antisense Oligonucleotide (AS ‐ODN) Technology: Principle, Mechanism and Challenges
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 16, 2020 Category: Biochemistry Authors: Seyed Mohammad Gheibi ‐Hayat, Khadijeh Jamialahmadi Tags: Review Article Source Type: research

Development of Rapid Gold Nanoparticles Based Lateral Flow Assays for Simultaneous Detection of Shigella and Salmonella Genus
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 16, 2020 Category: Biochemistry Authors: Mohammad Lukman Yahaya, Nor Dyana Zakaria, Rahmah Noordin, Khairunisak Abdul Razak Tags: Original Article Source Type: research

Antisense Oligonucleotide (AS ‐ODN) Technology: Principle, Mechanism and Challenges
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 16, 2020 Category: Biochemistry Authors: Seyed Mohammad Gheibi ‐Hayat, Khadijeh Jamialahmadi Tags: Review Article Source Type: research

Development of Rapid Gold Nanoparticles Based Lateral Flow Assays for Simultaneous Detection of Shigella and Salmonella Genus
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 16, 2020 Category: Biochemistry Authors: Mohammad Lukman Yahaya, Nor Dyana Zakaria, Rahmah Noordin, Khairunisak Abdul Razak Tags: Original Article Source Type: research

In situ product recovery (ISPR) techniques aiming to obtain biotechnological products: a glance to current knowledge
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 15, 2020 Category: Biochemistry Authors: Ariane G. Santos, Tiago L. Albuquerque, Bernardo D. Ribeiro, Maria Alice Z. Coelho Tags: Review Article Source Type: research

The effects of high ‐fat diet and metformin on urinary metabolites in diabetes and pre‐diabetes rat models
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 15, 2020 Category: Biochemistry Authors: Yan ‐Fen Lee, Xuan‐Yi Sim, Ying‐Hui Teh, Mohd Nazri Ismail, Peter Greimel, Vikneswaran Murugaiyah, Baharudin Ibrahim, Lay‐Harn Gam Tags: Original Article Source Type: research

In situ product recovery (ISPR) techniques aiming to obtain biotechnological products: a glance to current knowledge
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 15, 2020 Category: Biochemistry Authors: Ariane G. Santos, Tiago L. Albuquerque, Bernardo D. Ribeiro, Maria Alice Z. Coelho Tags: Review Article Source Type: research

The effects of high ‐fat diet and metformin on urinary metabolites in diabetes and pre‐diabetes rat models
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 15, 2020 Category: Biochemistry Authors: Yan ‐Fen Lee, Xuan‐Yi Sim, Ying‐Hui Teh, Mohd Nazri Ismail, Peter Greimel, Vikneswaran Murugaiyah, Baharudin Ibrahim, Lay‐Harn Gam Tags: Original Article Source Type: research

Sensitive identification of prostate ‐specific antigen by iron oxide nanoparticle antibody conjugates on the gap‐finger electrode surface
Identification of prostate specific antigen by iron oxide nanoparticle antibody conjugates. AbstractResearches have proved that increasing level of prostate ‐specific antigen (PSA) is an indicator for the progression of prostate cancer. The present study was focused to determine the PSA level by using anti‐PSA antibody conjugated iron oxide nanoparticles, as the probe immobilized on the gap‐fingered electrode sensing surface. The detection limit a nd sensitivity were found at the level of 1.9 pg/mL on the linear regression curve (y = 1.6939x− 0.5671;R² = 0.9878). A dose‐dependent liner range was found fr...
Source: Biotechnology and Applied Biochemistry - September 13, 2020 Category: Biochemistry Authors: Wei Zhang, Kai Li, Jingyang Guo, Tao Ma, Dongqing Wang, Sumei Shi, Subash C. B. Gopinath, Deqiang Gu Tags: Original Article Source Type: research

Biochemical features of dye ‐decolorizing peroxidases: Current impact on lignin degradation
Dye ‐decolorizing peroxidases can be exploited to degrade lignin and produce biofuel. AbstractDye ‐decolorizing peroxidases (DyP) were originally discovered in fungi for their ability to decolorize several different industrial dyes. DyPs catalyze the oxidation of a variety of substrates such as phenolic and nonphenolic aromatic compounds. Catalysis occurs in the active site or on the surface o f the enzyme depending on the size of the substrate and on the existence of radical transfer pathways available in the enzyme. DyPs show the typical features of heme‐containing enzymes with a Soret peak at 404–408 nm. The...
Source: Biotechnology and Applied Biochemistry - September 13, 2020 Category: Biochemistry Authors: Gianluca Catucci, Francesca Valetti, Sheila J. Sadeghi, Gianfranco Gilardi Tags: Review Article Source Type: research

In vitro and in silico anticancer evaluation of a medicinal mushroom, Ganoderma neo ‐japonicum Imazeki, against human colonic carcinoma cells
In this study, the anticancer properties ofG. neo ‐japonicum were evaluated using cellular and computational models. The ethanolic extract (EtOH) with a promising inhibitory effect was fractionated into four different fractions: hexane (Hex), chloroform (Chl), butanol (Btn), and aqueous (Aq). The active fractions were then subjected to cell apoptosis assessment and phytochemical profiling. Molecular docking was conducted to elucidate the affinity of selected constituents towards antiapoptotic Bcl ‐2 protein. The butanol fraction showed the highest antioxidant activities as well as total phenolic content. Both hexane an...
Source: Biotechnology and Applied Biochemistry - September 13, 2020 Category: Biochemistry Authors: Meng ‐Fei Lau, Kek‐Heng Chua, Vikineswary Sabaratnam, Umah Rani Kuppusamy Tags: Original Article Source Type: research

A thermophilic chitinase 1602 from the marine bacterium Microbulbifer sp. BN3 and its high ‐level expression in Pichia pastoris
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 13, 2020 Category: Biochemistry Authors: Ren Kuan Li, Ya Juan Hu, Yu Jie He, Tzi Bun Ng, Zhi Min Zhou, Xiu Yun Ye Tags: Original Article Source Type: research

Sensitive identification of prostate ‐specific antigen by iron oxide nanoparticle antibody conjugates on the gap‐finger electrode surface
Identification of prostate specific antigen by iron oxide nanoparticle antibody conjugates. AbstractResearches have proved that increasing level of prostate ‐specific antigen (PSA) is an indicator for the progression of prostate cancer. The present study was focused to determine the PSA level by using anti‐PSA antibody conjugated iron oxide nanoparticles, as the probe immobilized on the gap‐fingered electrode sensing surface. The detection limit a nd sensitivity were found at the level of 1.9 pg/mL on the linear regression curve (y = 1.6939x− 0.5671;R² = 0.9878). A dose‐dependent liner range was found fr...
Source: Biotechnology and Applied Biochemistry - September 13, 2020 Category: Biochemistry Authors: Wei Zhang, Kai Li, Jingyang Guo, Tao Ma, Dongqing Wang, Sumei Shi, Subash C. B. Gopinath, Deqiang Gu Tags: Original Article Source Type: research

A thermophilic chitinase 1602 from the marine bacterium Microbulbifer sp. BN3 and its high ‐level expression in Pichia pastoris
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 13, 2020 Category: Biochemistry Authors: Ren Kuan Li, Ya Juan Hu, Yu Jie He, Tzi Bun Ng, Zhi Min Zhou, Xiu Yun Ye Tags: Original Article Source Type: research

Production, characterization and immobilization of protease from the yeast Rhodotorula oryzicola
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 12, 2020 Category: Biochemistry Authors: Juliana Mota Oliveira, Pedro Fernandes, Raquel Guimar ães Benevides, Sandra Aparecida Assis Tags: Original Article Source Type: research

Immobilization of recombinant lysostaphin on nano ‐particle through biotin‐streptavidin conjugation technology as a geometrical progressed confrontation against staphylococcus aureus infection
This study aimed to design a hyper valency TiO2 nanocomposite as a reserved base to carry a high amount of active antibacterial agents such as lysostaphin via a biotin ‐streptavidin‐biotin bridge. The utilization of the Streptavidin‐Biotin system could increase the abundance of lysostaphin. Lysostaphin was expressed inE.coli and purified. Both recombinant lysostaphin and titanium oxide nanocomposite were conjugated with Biotin and linked to streptavidin bridge. The kinetic and activity of the enzyme were examined after each step utilizing N ‐acetylhexaglycine as a substrate. Physical characteristics of nanoparticle...
Source: Biotechnology and Applied Biochemistry - September 12, 2020 Category: Biochemistry Authors: Gelareh Ehsani, Maryam Farahnak, Dariush Norouzian, Parastoo Ehsani Tags: Original Article Source Type: research

Purification of the Phytase Enzyme from Lactobacillus plantarum: The Effect on Pansy Growth and Macro ‐Micro Element Content
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 12, 2020 Category: Biochemistry Authors: Fazilet Parlakova Karag öz, Yeliz Demir, Merve Şenol Kotan, Atilla Dursun, Şükrü Beydemir, Neslihan Dikbaş Tags: Original Article Source Type: research

Production, characterization and immobilization of protease from the yeast Rhodotorula oryzicola
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 12, 2020 Category: Biochemistry Authors: Juliana Mota Oliveira, Pedro Fernandes, Raquel Guimar ães Benevides, Sandra Aparecida Assis Tags: Original Article Source Type: research

Immobilization of recombinant lysostaphin on nano ‐particle through biotin‐streptavidin conjugation technology as a geometrical progressed confrontation against staphylococcus aureus infection
This study aimed to design a hyper valency TiO2 nanocomposite as a reserved base to carry a high amount of active antibacterial agents such as lysostaphin via a biotin ‐streptavidin‐biotin bridge. The utilization of the Streptavidin‐Biotin system could increase the abundance of lysostaphin. Lysostaphin was expressed inE.coli and purified. Both recombinant lysostaphin and titanium oxide nanocomposite were conjugated with Biotin and linked to streptavidin bridge. The kinetic and activity of the enzyme were examined after each step utilizing N ‐acetylhexaglycine as a substrate. Physical characteristics of nanoparticle...
Source: Biotechnology and Applied Biochemistry - September 12, 2020 Category: Biochemistry Authors: Gelareh Ehsani, Maryam Farahnak, Dariush Norouzian, Parastoo Ehsani Tags: Original Article Source Type: research

Purification of the Phytase Enzyme from Lactobacillus plantarum: The Effect on Pansy Growth and Macro ‐Micro Element Content
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 12, 2020 Category: Biochemistry Authors: Fazilet Parlakova Karag öz, Yeliz Demir, Merve Şenol Kotan, Atilla Dursun, Şükrü Beydemir, Neslihan Dikbaş Tags: Original Article Source Type: research

Synthesis and characterization of V2O5/urease for biosensor of urea
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 11, 2020 Category: Biochemistry Authors: Renata Cristina Felix da Rocha, Fl ávia Alves Souza, Noemy Santana Vieira, Dane Tadeu Cestarolli, Elidia Maria Guerra Tags: Original Article Source Type: research

Issue Information
Biotechnology and Applied Biochemistry, Volume 67, Issue 4, Page 457-460, July/August 2020. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 10, 2020 Category: Biochemistry Tags: Issue Information Source Type: research

Special issue (67:4): Synthetic and engineered enzymes for biocatalysis and biotransformation
Biotechnology and Applied Biochemistry, Volume 67, Issue 4, Page 461-462, July/August 2020. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 10, 2020 Category: Biochemistry Authors: Angela Lombardi, Lionel Cheruzel, Long Liu Tags: Editorial Source Type: research

Biotransformation and chiral resolution of d,l ‐alanine into pyruvate and d‐alanine with a whole‐cell biocatalyst expressing l‐amino acid deaminase
Pyruvate production by whole ‐cell biocatalysis AbstractPyruvate is an important pharmaceutical intermediate and is widely used in food, nutraceuticals, and pharmaceuticals. However, high environmental pollution caused by chemical synthesis or complex separation process of microbial fermentation methods constrain the supply of pyruvate. Here, one ‐step pyruvate andd‐alanine production fromd,l‐alanine by whole‐cell biocatalysis was investigated. First,l‐amino acid deaminase (Pm1) fromProteus mirabilis was expressed inEscherichia coli, resulting in pyruvate titer of 12.01  g/L. Then, N‐terminal coding sequ...
Source: Biotechnology and Applied Biochemistry - September 10, 2020 Category: Biochemistry Authors: Ke Liu, Mengyue Gong, Xueqin Lv, Jianghua Li, Guocheng Du, Long Liu Tags: Original Article Source Type: research

Proteomic exploration of soft and hard bio ‐corona onto PEGylated multi‐walled carbon nanotubes
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 10, 2020 Category: Biochemistry Authors: Maria Nicoletti, Cristian Gambarotti, Elisa Fasoli Tags: Original Article Source Type: research

Issue Information
Biotechnology and Applied Biochemistry, Volume 67, Issue 4, Page 457-460, July/August 2020. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 10, 2020 Category: Biochemistry Tags: Issue Information Source Type: research

Special issue (67:4): Synthetic and engineered enzymes for biocatalysis and biotransformation
Biotechnology and Applied Biochemistry, Volume 67, Issue 4, Page 461-462, July/August 2020. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 10, 2020 Category: Biochemistry Authors: Angela Lombardi, Lionel Cheruzel, Long Liu Tags: Editorial Source Type: research

Proteomic exploration of soft and hard bio ‐corona onto PEGylated multi‐walled carbon nanotubes
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 10, 2020 Category: Biochemistry Authors: Maria Nicoletti, Cristian Gambarotti, Elisa Fasoli Tags: Original Article Source Type: research

Green synthesis of silver and gold nanoparticles using Crataegus oxyacantha extract and their urease inhibitory activities
This study reports the green synthesis and urease inhibitory activities of Ag and Au nanoparticles (NPs) usingCrataegus oxyacantha extract. The as ‐synthesized NPs were characterized by UV‐Visible, FT‐IR spectroscopy, Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). The obtained NPs were spherical in shape and their size was around 85 nm. A strong correlation between the phytochemicals present in the extract and thei r capability for the synthesis of NPs was observed. Furthermore, the shape, size, stability and bioactivity of the NPs were strongly influenced by the stabilizing phytochemicals. The...
Source: Biotechnology and Applied Biochemistry - September 4, 2020 Category: Biochemistry Authors: Shujat Ali, Misal Bacha, Muhammad Raza Shah, Wahid Shah, Khadija Tul Kubra, Ajmal Khan, Manzoor Ahmad, Abdul Latif, Mumtaz Ali Tags: Original Article Source Type: research

Secondary metabolite contents and antimicrobial activity of leaf extracts reveal genetic variability of Vernonia amygdalina and Vernonia calvoana morphotypes
This study was therefore aimed at evaluating the genetic variability ofVernonia amygdalina (VAA and VALF) andV.calvoana (VCAB, VCAV, VCSB and VCSV) morphotypes in 2016 based on secondary metabolite contents and antimicrobial properties. This involved phytochemical analysis by HPLC/MS for the detection of alkaloids, flavonoids, phenols, and tannins in leaves from each of the six genotypes. Results showed that all tested genotypes are rich in phenols (18 analyzed), flavonoids and tannins with, VAA richer in phenols (206.1 ± 3.12 μg/g of dry extract), followed by VCAV (197.9 ± 18.03 μg/g). The lowest level...
Source: Biotechnology and Applied Biochemistry - September 3, 2020 Category: Biochemistry Authors: Nguimkeng Gaintse Eric Dumas, Ngandjui Tchangoue Yvan Anderson, Ntsomboh ‐Ntsefong Godswill, Muthu Thiruvengadam, Gheldiu Ana‐Maria, Paltinean Ramona, Gianina Cristina Crisan, Vlase Laurian, Mohammad Ali Shariati, Zhaiyk Tokhtarov, Youmbi Em Tags: Original Article Source Type: research

Microbial lipase production: A deep insight into the recent advances of lipase production and purification techniques
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 3, 2020 Category: Biochemistry Authors: Samar Fatima, Amna Faryad, Asia Ataa, Faiz Ahmad Joyia, Aqsa Parvaiz Tags: Review Article Source Type: research

Secondary metabolite contents and antimicrobial activity of leaf extracts reveal genetic variability of Vernonia amygdalina and Vernonia calvoana morphotypes
This study was therefore aimed at evaluating the genetic variability ofVernonia amygdalina (VAA and VALF) andV.calvoana (VCAB, VCAV, VCSB and VCSV) morphotypes in 2016 based on secondary metabolite contents and antimicrobial properties. This involved phytochemical analysis by HPLC/MS for the detection of alkaloids, flavonoids, phenols, and tannins in leaves from each of the six genotypes. Results showed that all tested genotypes are rich in phenols (18 analyzed), flavonoids and tannins with, VAA richer in phenols (206.1 ± 3.12 μg/g of dry extract), followed by VCAV (197.9 ± 18.03 μg/g). The lowest level...
Source: Biotechnology and Applied Biochemistry - September 3, 2020 Category: Biochemistry Authors: Nguimkeng Gaintse Eric Dumas, Ngandjui Tchangoue Yvan Anderson, Ntsomboh ‐Ntsefong Godswill, Muthu Thiruvengadam, Gheldiu Ana‐Maria, Paltinean Ramona, Gianina Cristina Crisan, Vlase Laurian, Mohammad Ali Shariati, Zhaiyk Tokhtarov, Youmbi Em Tags: Original Article Source Type: research

Microbial lipase production: A deep insight into the recent advances of lipase production and purification techniques
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - September 3, 2020 Category: Biochemistry Authors: Samar Fatima, Amna Faryad, Asia Ataa, Faiz Ahmad Joyia, Aqsa Parvaiz Tags: Review Article Source Type: research

Activity prediction of aminoquinoline drugs based on deep learning
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - August 31, 2020 Category: Biochemistry Authors: Wenle Wang, Jinquan Chen, Yujie Zhu, Feng Feng Tags: Original Article Source Type: research

Biotransformation and chiral resolution of d,l ‐alanine into pyruvate and d‐alanine with a whole‐cell biocatalyst expressing l‐amino acid deaminase
Pyruvate production by whole ‐cell biocatalysis AbstractPyruvate is an important pharmaceutical intermediate and is widely used in food, nutraceuticals, and pharmaceuticals. However, high environmental pollution caused by chemical synthesis or complex separation process of microbial fermentation methods constrain the supply of pyruvate. Here, one ‐step pyruvate andd‐alanine production fromd,l‐alanine by whole‐cell biocatalysis was investigated. First,l‐amino acid deaminase (Pm1) fromProteus mirabilis was expressed inEscherichia coli, resulting in pyruvate titer of 12.01  g/L. Then, N‐terminal coding sequ...
Source: Biotechnology and Applied Biochemistry - August 31, 2020 Category: Biochemistry Authors: Ke Liu, Mengyue Gong, Xueqin Lv, Jianghua Li, Guocheng Du, Long Liu Tags: Original Article Source Type: research

Phlorotannins as HIV Vpu inhibitors, an in silico virtual screening study of marine natural products
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - August 29, 2020 Category: Biochemistry Authors: Mohammad Amin Langarizadeh, Ardavan Abiri, Saeid Ghasemshirazi, Nazanin Foroutan, Arash Khodadadi, Ehsan Faghih ‐Mirzaei Tags: Original Article Source Type: research

Biochemical features of dye ‐decolorizing peroxidases: current impact on lignin degradation
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - August 29, 2020 Category: Biochemistry Authors: Gianluca Catucci, Francesca Valetti, Sheila J. Sadeghi, Gianfranco Gilardi Tags: Review Article Source Type: research

Phlorotannins as HIV Vpu inhibitors, an in silico virtual screening study of marine natural products
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - August 29, 2020 Category: Biochemistry Authors: Mohammad Amin Langarizadeh, Ardavan Abiri, Saeid Ghasemshirazi, Nazanin Foroutan, Arash Khodadadi, Ehsan Faghih ‐Mirzaei Tags: Original Article Source Type: research

In vitro and in silico anticancer evaluation of a medicinal mushroom, Ganoderma neo ‐japonicum Imazeki, against human colonic carcinoma cells
This article is protected by copyright. All rights reserved (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - August 28, 2020 Category: Biochemistry Authors: Meng ‐Fei Lau, Kek‐Heng Chua, Vikineswary Sabaratnam, Umah Rani Kuppusamy Tags: Original Article Source Type: research

In vitro CRISPR/Cas9 system for genome editing of Aspergillus niger based on removable bidirectional selection marker AmdS
This study confirmed the facts that the endogenousamdS can be used as a dominant marker and that it can be removed by counter ‐selection in gene editing ofA. niger. The proposedin vitro CRISPR/Cas9 method offers a powerful tool for marker ‐free genetic manipulation of filamentous fungi industrial‐specific strains. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - August 26, 2020 Category: Biochemistry Authors: Yilin Nan, Liming Ouyang, Ju Chu Tags: Original Article Source Type: research

Sensitive determination of NT ‐proBNP for diagnosing abdominal aortic aneurysms incidence on interdigitated electrode sensor
Diagnosing abdominal aortic aneurysms incidence on inderdigitated electrode sensor. AbstractAbdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health ‐related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immediate treatment. Blood‐based biomarker helps to diagnose AAA and to monitor the condition after AAA surgery. N‐terminal pro‐B‐type natriuretic pepti de (NT‐proBNP) is a hormone produced in the heart in small q...
Source: Biotechnology and Applied Biochemistry - August 25, 2020 Category: Biochemistry Authors: Changcheng Qiu, Xiao Wang, Xia Zhang, Zhenhuan Li, Yiming Zhou, Jie Kang Tags: Original Article Source Type: research

Aptamer –17β‐estradiol–antibody sandwich ELISA for determination of gynecological endocrine function
In this study, the performance of an enzyme‐linked immunosorbent assay (ELISA) for 17β‐E2 quantifi cation was enhanced by using a gold nanoparticle (GNP)‐conjugated aptamer. An anti‐17β‐E2–aptamer–GNP antibody was immobilized on an amine‐modified ELISA surface. Then, 17β‐E2 was allowed to interact with and be sandwiched by antibodies. Aptamer–GNP conjugation was confirmed by col orimetric assays via the naked eye and UV–visible light spectroscopy. The detection limit based on a signal‐to‐noise ratio (S/N) of 3 was estimated to be 1.5  nM (400 pg/mL), and th...
Source: Biotechnology and Applied Biochemistry - August 25, 2020 Category: Biochemistry Authors: Yue Huang, Li Zhang, Zhenzuo Li, Subash C. B. Gopinath, Yeng Chen, Yan Xiao Tags: Original Article Source Type: research