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Poly ‐γ‐glutamic acid overproduction of Bacillus licheniformis ATCC 9945a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches
This study optimized the poly-γ-glutamic acid production ofBacillus licheniformis ATCC 9945a in several steps. At first, the most critical components of the culture medium, includingl-glutamic acid, citric acid, and glycerol, were selected by screening nine factors through the Plackett –Burman experimental design and then were optimized using the response surface method and the central composite design algorithm. Under optimal conditions, the production of poly-γ-glutamic acid increased by more than 4.2 times from 11.2 to 47.2 g/L. This is one of the highest production rates o f this strain in submerged batch fermenta...
Source: Biotechnology and Applied Biochemistry - January 22, 2024 Category: Biochemistry Authors: Mehrdad Ebrahimzadeh Kouchesfahani, Ali Bahrami, Valiollah Babaeipour Tags: ORIGINAL ARTICLE Source Type: research

The importance, benefits, and future of nanobiosensors for infectious diseases
AbstractInfectious diseases, caused by pathogenic microorganisms such as bacteria, viruses, parasites, or fungi, are crucial for efficient disease management, reducing morbidity and mortality rates and controlling disease spread. Traditional laboratory-based diagnostic methods face challenges such as high costs, time consumption, and a lack of trained personnel in resource-poor settings. Diagnostic biosensors have gained momentum as a potential solution, offering advantages such as low cost, high sensitivity, ease of use, and portability. Nanobiosensors are a promising tool for detecting and diagnosing infectious diseases ...
Source: Biotechnology and Applied Biochemistry - January 19, 2024 Category: Biochemistry Authors: Th. S. Dhahi, Alaa Kamal Yousif Dafhalla, Sawsan Ali Saad, Duria Mohammed Ibrahim Zayan, Amira Elsir Tayfour Ahmed, Mohamed Elshaikh Elobaid, Tijjani Adam, Subash C. B. Gopinath Tags: REVIEW ARTICLE Source Type: research

Overproduction of bacterial cellulose from Acetobacter xylinum BPR2001 using food industries wastes
In this study, a cost-effective complex culture media containing molasses and corn steep liquor (CSL) was developed for the high production of bacterial cellulose (BC) by investigating the effect of four effective factors on BC production at three levels using Taguchi and combined methods. The predicted and actual values of BC production in optimal conditions by Taguchi and combined methods were 8.41 and 14.52  g/L, respectively. These results showed that the combined method was more suitable for predicting the optimal conditions in the optimization of BC production, the cost of developed culture medium was around 94% cos...
Source: Biotechnology and Applied Biochemistry - January 18, 2024 Category: Biochemistry Authors: Sirwan Khanchezar, Valiolah Babaeipour, Atiyeh Sadat Mostafa Tags: ORIGINAL ARTICLE Source Type: research

Construction and characterization of a promoter library with varying strengths to enhance acetoin production from xylose in Serratia marcescens
The objective of this study was to utilize a constitutive promoter screening strategy to enhance both xylose utilization and acetoin production inS. marcescens HBQA-7. By utilizing RNA-seq, we identified the endogenous constitutive promoter P6 that is the most robust, which facilitated the overexpression of the sugar transporter protein GlfL445I, α-acetyl lactate synthase, and α-acetyl lactate decarboxylase, respectively. The resultant recombinant strains exhibited enhanced xylose utilization rates and acetoin yields. Subsequently, a recombinant plasmid, denoted as pBBR1MCS-P6-glfL445IalsSalsD, was constructed, simultane...
Source: Biotechnology and Applied Biochemistry - January 16, 2024 Category: Biochemistry Authors: Jie Liu, Di Liu, Tingting Sun, Tai ‐Ping Fan, Yujie Cai Tags: ORIGINAL ARTICLE Source Type: research

Biotechnological insights into extracellular enzyme production by thermotolerant fungi from hot springs and caves: Morphology, pellets formation, and protease production
This study investigates the thermotolerant fungal biodiversity in caves and hot springs, focusing on their potential for extracellular enzyme production, specifically proteases. Samples were collected from the Cardonal region in Hidalgo, Mexico, using three different isolation methods. The study characterizes the morphological diversity of the isolated fungi and identifies various genera, includingAspergillus,Penicillium, Trichoderma, Cladosporium, andFusarium, based on morphology. The isolated fungi were screened for their ability to produce extracellular enzymes on solid media, with a particular emphasis on proteases due...
Source: Biotechnology and Applied Biochemistry - January 16, 2024 Category: Biochemistry Authors: Adriana Jazm ín Legorreta‐Castañeda, Guadalupe Guerra‐Sánchez, Karina García‐Gutiérrez, Dario Rafael Olicón‐Hernández Tags: ORIGINAL ARTICLE Source Type: research

Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches
AbstractGlobally, people are in great threat due to the highly spreading of viral infectious diseases. Every year like 100 –300 million cases of infections are found, and among them, above 80% are not recognized and irrelevant. Dengue virus (DENV) is an arbovirus infection that currently infects people most frequently. DENV encompasses four viral serotypes, and they each express comparable sign. From a mild febrile si ckness to a potentially fatal dengue hemorrhagic fever, dengue can induce a variety of symptoms. Presently, the globe is being challenged by the untimely identification of dengue infection. Therefore, this ...
Source: Biotechnology and Applied Biochemistry - January 16, 2024 Category: Biochemistry Authors: Shadan Raza, Renu Poria, Deepak Kala, Nishant Sharma, Anil K. Sharma, Nkurunziza Florien, Hardeep S. Tuli, Ankur Kaushal, Shagun Gupta Tags: REVIEW ARTICLE Source Type: research

Enzymatic properties of ornithine decarboxylase from Clostridium aceticum DSM1496
In this study, we expressed ODC derived fromC. aceticum DSM1496 inEscherichia coli BL21 (DE3) and thoroughly examined its enzymatic properties. The enzyme has a molecular weight of 55.27  kDa and uses pyridoxal-5′-phosphate (PLP) as a coenzyme with aKm = 0.31 mM. ODC exhibits optimal activity at pH 7.5, and it maintains high stability even at pH 4.5. The peak reaction temperature for ODC is 30°C. Besides, it can be influenced by certain metal ions such as Mn2+. Althoughl-ornithine serves as the preferred substrate for ODC, the enzyme also decarboxylatesl-arginine andl-lysine simultaneously. The results indicate that...
Source: Biotechnology and Applied Biochemistry - January 16, 2024 Category: Biochemistry Authors: Qian Tan, Linbo Gou, Tai ‐Ping Fan, Yujie Cai Tags: ORIGINAL ARTICLE Source Type: research