In vitro antibacterial activity of antibiotics and plant essential oils against Escherichia coli MTCC443 supported through the molecular docking and pharmacokinetics study

This study has been made to compare the in vitro antibacterial activity and further validated the same through the molecular docking study of 13 antibiotics such as ciprofloxacin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, kanamycin, vancomycin, streptomycin, penicillin, nalidixic acid, trimethoprim, and polymyxin, and 10 EOs such as garlic, tulsi, neem, clove, thyme, peppermint, coriander, tea, lavender, and eucalyptus against the target protein (DNA gyrase) ofE. coli MTCC443.E. coli Microbial Type Culture Collection 443 was found to be highly sensitive to ciprofloxacin (zone of inhibition [ZOI], 2.5  cm ±0.1) and chloramphenicol (ZOI, 1.8 cm ±0.1), whereas garlic oil (ZOI, 5.5 cm ±0.1) and coriander oil (ZOI, 4.4 cm ±0.1) were found comparatively most effective. Further, the in silico investigation observed the same; ciprofloxacin (binding affinity: −7.2 kcal/mol) and chloramphenicol (binding affinity: −6.6 kcal/mol). Penicillin (binding affinity: −4.2 kcal/mol) and polymyxin (binding affinity: −0.3 kcal/mol) were found to be least effective against the tested microbe, whereas vancomycin (binding affinity: +0.8 kcal/mol) had no effect on it. Garlic (binding affinity: −7.8 kcal/mol), coriander (binding affinity: −6.8 kcal/mol), peppermint (binding affinity: −6.2 kcal/mol), and neem (binding affinity: −6.2 kcal/mol) oil exhibited the potent antibacterial activity againstE. coli MTCC443, whereas thyme (binding affinity: ...
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: ORIGINAL ARTICLE Source Type: research